FastQCFastQC Report
Wed 25 May 2016
SRR1295347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837743
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT153940.40112117981845063No Hit
GGTATCAACGCAGAGTACTTTTTTT134130.3495022986166609No Hit
TATCAACGCAGAGTACTTTTTTTTT90920.23691007970049063No Hit
GTGGTATCAACGCAGAGTACATGGG63920.16655622849159No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61740.16087580643101948No Hit
GTACATAAGCAGTGGTATCAACGCA43010.1120710792775858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3150.015.0896023
CAGCGTC3450.013.5019132
GCAGCGT3400.011.7567541
GATTACG600.005848706211.09085752
GCGTCAG4600.010.539764
ACCGCCC750.002656814610.1297768
GTTCTAG4100.09.9816341
TAGGACC2151.0913936E-119.7275294
CGTCAGA5000.09.506455
GGTATCA55800.09.483261
TCTGTCG2750.08.9786658
CTAGCAC3400.08.9472473
GTCTTAG3750.08.8828811
GCTTATG8200.08.7049131
CCGCCCG1000.00291566768.54699817
GTCGCCC3150.08.4414811
GTCTTAC4000.08.3277011
GTACTAG3001.8189894E-128.248391
CTATACT7650.08.2016424
GTATTAG6850.08.1974451
Produced by FastQC (version 0.11.5)