FastQCFastQC Report
Wed 25 May 2016
SRR1295346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123170
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG49300.15785243838791996No Hit
GTATCAACGCAGAGTACTTTTTTTT43070.13790475702571425No Hit
GGTATCAACGCAGAGTACTTTTTTT36190.11587585690180169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1403.6379788E-1212.9004711
CGTCAGA1603.6379788E-1211.8759212
GGTATCA30600.09.5677381
AGCACCG800.00451173039.5014985
GCACCGT800.004521289.49906356
GTGGTAT15800.09.1446371
CGAATGT850.0074472188.93986515
GTCCTAG3050.08.7264611
TGGTATC15600.08.7090092
GTCTTGG4300.08.6213671
TCTACAC4100.08.3426453
GGGCGAC1150.00111229628.25992311
TATTCCG1401.0377115E-48.1441415
TACCCTA2002.3016219E-78.0762745
TAGACTG4250.08.0483275
GTCTAAG2751.9463187E-107.9501471
ATTATAC6150.07.72467143
GTATCAA51550.07.6708711
CTAGGAC3104.0017767E-117.6623763
TAGGACC2501.7391358E-87.6011984
Produced by FastQC (version 0.11.5)