##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295346_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3123170
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.78285876209108	31.0	30.0	33.0	25.0	34.0
2	29.767372893566474	31.0	30.0	33.0	25.0	34.0
3	30.141058924105955	31.0	30.0	34.0	26.0	34.0
4	33.49570820672586	35.0	33.0	37.0	27.0	37.0
5	33.69243653083245	35.0	35.0	37.0	28.0	37.0
6	33.860361107464534	35.0	35.0	37.0	28.0	37.0
7	33.772709458658994	35.0	35.0	37.0	28.0	37.0
8	33.790178248382254	35.0	35.0	37.0	28.0	37.0
9	34.764704450926466	38.0	34.0	39.0	27.0	39.0
10	34.66859633001086	38.0	34.0	39.0	27.0	39.0
11	35.06532177242993	38.0	35.0	39.0	27.0	39.0
12	35.06394176429717	38.0	35.0	39.0	27.0	39.0
13	34.95955679646001	38.0	35.0	39.0	27.0	39.0
14	35.09306345796098	38.0	33.0	40.0	24.0	41.0
15	35.71503088208455	39.0	34.0	41.0	27.0	41.0
16	35.81365727770182	39.0	34.0	41.0	27.0	41.0
17	35.63619079332857	39.0	34.0	41.0	26.0	41.0
18	35.61483108508342	39.0	34.0	41.0	26.0	41.0
19	35.67473624554539	39.0	34.0	41.0	26.0	41.0
20	35.64585245119542	39.0	34.0	41.0	26.0	41.0
21	35.63796431190105	39.0	34.0	41.0	26.0	41.0
22	35.37965464576056	39.0	34.0	40.0	25.0	41.0
23	35.058672758767536	39.0	33.0	40.0	24.0	41.0
24	35.38035777751451	39.0	34.0	40.0	25.0	41.0
25	33.98539528748035	38.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4079087190486774
1101	2	0.674109219343336
1101	3	0.5837984050698779
1101	4	0.4417466693839103
1101	5	0.384084069914465
1101	6	0.4456343301388941
1101	7	0.3347420373059933
1101	8	0.30472441585590104
1101	9	0.3583562402373346
1101	10	0.6046911765384948
1101	11	0.5445539920970361
1101	12	0.509047614214488
1101	13	0.5000373870734265
1101	14	1.0440880027635586
1101	15	0.7962558941742017
1101	16	0.632335608331978
1101	17	0.9299036896118338
1101	18	0.9124534605407604
1101	19	0.8924336999318925
1101	20	1.016059099449457
1101	21	1.0880139656496013
1101	22	0.7079823508325944
1101	23	-1.722224628042106
1101	24	0.03131269597409414
1101	25	0.5002912311114187
1102	1	0.0855771722313996
1102	2	0.11001753881645016
1102	3	0.11909632071819942
1102	4	0.36243264370307315
1102	5	-0.11980138617987279
1102	6	0.016744925091323637
1102	7	-0.043429150282946694
1102	8	0.11835600727810913
1102	9	0.17277838579736482
1102	10	0.7510776425638355
1102	11	0.3936879030945022
1102	12	0.24257984301726765
1102	13	0.3234419378623983
1102	14	0.8445018411851137
1102	15	0.5948802756816036
1102	16	0.29524269620674914
1102	17	0.657480676384921
1102	18	0.576258330842883
1102	19	0.5814784892902338
1102	20	0.7323605734821399
1102	21	0.6747440915563132
1102	22	0.619581533522279
1102	23	0.6934555072555639
1102	24	0.610067166435158
1102	25	0.38023864174333966
1103	1	-0.3237965237947016
1103	2	-1.6263253112055445
1103	3	-0.33308642538238686
1103	4	0.021125205127916047
1103	5	-0.19454965431372528
1103	6	-0.12754232395565168
1103	7	-0.07733766394623132
1103	8	0.0811905090349967
1103	9	0.7246966081068607
1103	10	0.8904312680124988
1103	11	0.42969599174490725
1103	12	0.29756718325980813
1103	13	0.1903587157700315
1103	14	-1.0515805255947797
1103	15	-0.18451519021824225
1103	16	-0.06338245979809187
1103	17	0.4040438496130747
1103	18	0.6012096648848839
1103	19	0.5253903826650514
1103	20	0.5624347194441413
1103	21	0.6763867446487453
1103	22	0.24515292190400828
1103	23	1.0460915878475205
1103	24	0.9038868257828909
1103	25	0.8588624383111423
1104	1	-0.17080570816843732
1104	2	-0.12909653927909304
1104	3	-0.6236537765036267
1104	4	-0.5212500816050678
1104	5	-0.43517933148286403
1104	6	-0.2740390192230109
1104	7	-0.2566277164071309
1104	8	-0.2532456900616822
1104	9	-2.315103793039185
1104	10	-0.7968881768226055
1104	11	-0.6808660952206864
1104	12	-0.2987487310236361
1104	13	-0.1421474911085383
1104	14	-2.0913714137575354
1104	15	-1.0168039019430637
1104	16	-0.446894401679252
1104	17	-0.13999250461841228
1104	18	-0.04286764548373867
1104	19	0.020627419453084883
1104	20	0.12173488402332566
1104	21	0.31602332124621313
1104	22	0.3888857296450112
1104	23	0.9137977766148424
1104	24	0.47968474440617825
1104	25	0.2205712628151204
1105	1	-0.2348187193411242
1105	2	-0.2714973178332123
1105	3	-1.5396027425966565
1105	4	-0.681528738636338
1105	5	-0.5746451707733513
1105	6	-0.2942025777759554
1105	7	-0.3199953667316322
1105	8	-0.26665978399821455
1105	9	0.17174847636300683
1105	10	0.4396895264845071
1105	11	-0.2631783536193453
1105	12	-0.25163998799916953
1105	13	-0.29203238566889667
1105	14	0.31974674375719303
1105	15	0.08765484724217032
1105	16	-0.3510643488683485
1105	17	0.16637717353884796
1105	18	0.11788776920561617
1105	19	-0.019482643669853417
1105	20	-0.4980152184821236
1105	21	-0.0557324190009183
1105	22	0.006956158255853495
1105	23	0.1438868327919991
1105	24	0.18789768749714852
1105	25	0.36195513162485327
1106	1	-1.7699630392173837
1106	2	-1.0563307531868986
1106	3	-1.1052391323027706
1106	4	-1.5224898536490876
1106	5	-0.9006550910512416
1106	6	-0.8332624788209557
1106	7	-0.7236915364393468
1106	8	-0.8513768572501803
1106	9	-0.3659132820888118
1106	10	-1.9585157716417143
1106	11	-1.302561527856433
1106	12	-0.9331616679677452
1106	13	-1.0835713914964415
1106	14	-0.3188835344760719
1106	15	-0.8539160238681447
1106	16	-1.3445652731443332
1106	17	-0.7719988932576101
1106	18	-0.7390335570562883
1106	19	-0.6199408416586607
1106	20	-0.37201417336584086
1106	21	-0.47892348031183474
1106	22	-0.39184785786647325
1106	23	-0.7091714626001817
1106	24	-0.9276717424656411
1106	25	-1.4609694006622505
1107	1	-0.09196024562456984
1107	2	0.08463284126160886
1107	3	-0.07186608916007131
1107	4	0.06609049555773794
1107	5	-0.19328282781228978
1107	6	-0.06978871452803048
1107	7	-0.21547029254115557
1107	8	-0.263610549478301
1107	9	0.40313361681843674
1107	10	-0.005813273825175713
1107	11	0.008441411364763951
1107	12	-0.16002602034054547
1107	13	0.04127723416945628
1107	14	-1.2185357742754235
1107	15	-0.5390310451851263
1107	16	-0.24003335252223224
1107	17	-0.023698390047798057
1107	18	0.3132046392221923
1107	19	0.14571594815863165
1107	20	0.3059071629696746
1107	21	0.3952761912710443
1107	22	-0.27439059709393376
1107	23	0.648049464932889
1107	24	0.31358099386083893
1107	25	-0.018214538431728045
1108	1	-0.37512102486317644
1108	2	0.03135872535623818
1108	3	-0.24317541688539634
1108	4	-0.4758193155151389
1108	5	-0.3889695882072033
1108	6	-0.2632953862928815
1108	7	-0.23873739843642738
1108	8	-0.42764662406872844
1108	9	0.09220302572261829
1108	10	0.16761253771830553
1108	11	-0.17250851853513183
1108	12	-0.22431908778370513
1108	13	-0.3530672073337229
1108	14	-0.10614901290009016
1108	15	-0.26754842804932366
1108	16	-0.3305237591028174
1108	17	0.13715303205285068
1108	18	0.13290239994064734
1108	19	0.16989692897178799
1108	20	0.1788330823594606
1108	21	0.14785739463977166
1108	22	0.09062295722629443
1108	23	-0.0851929343533655
1108	24	0.022570596496215956
1108	25	-0.8216498110377799
1109	1	-0.9242511477221136
1109	2	-0.6984652067275832
1109	3	-0.7057994715292253
1109	4	-0.6681914495539374
1109	5	-1.0199843701400653
1109	6	-1.2966586123114752
1109	7	-0.860786990401273
1109	8	-1.2583886352485933
1109	9	-0.9926507243147427
1109	10	-1.2723095171048797
1109	11	-1.2965586784609968
1109	12	-1.2703448552783954
1109	13	-1.315176734132514
1109	14	-0.8265481549800242
1109	15	-1.365841929379549
1109	16	-1.4627465791787486
1109	17	-1.4813494191473424
1109	18	-2.2362696474093724
1109	19	-2.1705504897948344
1109	20	-1.803184225505305
1109	21	-1.6391340560522423
1109	22	-1.8150084510181088
1109	23	-1.2237836437067813
1109	24	-2.253781673853233
1109	25	-1.260791666775681
1110	1	0.07704222523933524
1110	2	0.17984764304255307
1110	3	0.036364912031288554
1110	4	0.23748376487037604
1110	5	0.11236827915561065
1110	6	0.11005040505724395
1110	7	0.0707506490668024
1110	8	0.08471885752126695
1110	9	0.3515305354026239
1110	10	0.1818079079060837
1110	11	0.18407920562309243
1110	12	0.2785506382964371
1110	13	0.2812885911218572
1110	14	-2.4829657157200486
1110	15	-0.7172529805467178
1110	16	-0.16234131489000703
1110	17	-0.2085037605399478
1110	18	-2.894834411933914
1110	19	-0.5832112757904255
1110	20	-2.1402233793759606
1110	21	-0.3521120080953537
1110	22	-1.8999610279618153
1110	23	-0.4735849893940056
1110	24	0.10987668339557644
1110	25	0.016687505417756654
1111	1	-0.1772408764815232
1111	2	-1.302321680942022
1111	3	-0.3262840682362729
1111	4	-0.02392036469441905
1111	5	-0.040431314656395045
1111	6	0.06659746686977996
1111	7	0.07352995919200822
1111	8	0.04680709422902396
1111	9	-1.5820114828825567
1111	10	-0.24695477533195742
1111	11	-0.12828616894668698
1111	12	0.018662237143459492
1111	13	0.09151813599785896
1111	14	0.9227251094670095
1111	15	0.29290614997044884
1111	16	0.34512302251186355
1111	17	-0.1636499721979945
1111	18	0.1536027947759422
1111	19	-0.029070011532262185
1111	20	0.40857030991514875
1111	21	-0.5070688921571218
1111	22	-0.18655162206714238
1111	23	-0.03756950852030627
1111	24	-0.38670305780000547
1111	25	-1.8247589400120106
1112	1	0.2941126916119181
1112	2	0.42010530444764527
1112	3	0.1538702864534116
1112	4	0.4753492668603414
1112	5	0.25027370681195293
1112	6	0.26674292788336373
1112	7	0.19306610457397255
1112	8	0.06157751386230359
1112	9	-0.26687059223030474
1112	10	0.6552493740620378
1112	11	0.2294236106383778
1112	12	0.25860373651843105
1112	13	0.14633618620760558
1112	14	0.593419952617154
1112	15	0.22384319756643833
1112	16	0.2962797439075828
1112	17	-3.6375801370768706
1112	18	-0.8910535387566725
1112	19	-1.1552987469997476
1112	20	-0.007009829517933497
1112	21	-0.42563740557916674
1112	22	-1.5277199546511042
1112	23	-1.468315539717345
1112	24	-0.21976519989130594
1112	25	0.1242793077361597
1113	1	-0.26304712426468413
1113	2	-0.054134378989324716
1113	3	-0.05179823759837987
1113	4	-0.5891227243103501
1113	5	-0.17070586195167436
1113	6	-0.22116684088565108
1113	7	-0.33245975076533796
1113	8	-0.2742996295332105
1113	9	0.20826144362530385
1113	10	0.3579358528366967
1113	11	-0.06857929657312667
1113	12	-0.12431908778370371
1113	13	-0.3277484459493891
1113	14	-1.4376966319477091
1113	15	-1.6407822297667352
1113	16	-0.35186125585015304
1113	17	-0.2546990366434798
1113	18	-0.450310584706763
1113	19	-0.029260630226751516
1113	20	0.4447256142356011
1113	21	-1.544954210876746
1113	22	-1.902471630335512
1113	23	-0.6480409697423823
1113	24	-2.3482822528402423
1113	25	-0.6200462461145477
1114	1	0.14614872442473015
1114	2	0.2134872486475956
1114	3	-0.08986571524413023
1114	4	0.35530039590687323
1114	5	0.19022344661409107
1114	6	0.22878435591240986
1114	7	0.21058063216902667
1114	8	0.09549315627841537
1114	9	0.48998491969197744
1114	10	0.3133433878460039
1114	11	0.392067552057938
1114	12	0.20657672427880414
1114	13	0.3192620205392558
1114	14	1.0932207958694704
1114	15	0.5490541961952715
1114	16	0.4304343553901617
1114	17	-0.2846886532401598
1114	18	-2.0814548156522505E-4
1114	19	-0.5133085877980506
1114	20	-1.5573457887769138
1114	21	-0.7560615723417783
1114	22	-1.4614307310959873
1114	23	-2.7433985661006233
1114	24	-2.074054701295303
1114	25	-1.2850794417916376
1115	1	0.4349672528721591
1115	2	0.515540883067807
1115	3	0.4286043556296697
1115	4	0.5232278791202916
1115	5	0.5063502063772987
1115	6	0.39650004703196373
1115	7	0.4891952897903735
1115	8	0.5264691131352421
1115	9	0.8055200191866732
1115	10	1.0075359313181664
1115	11	0.6552055974142803
1115	12	0.5299335223512571
1115	13	0.534641815669417
1115	14	1.24609506654987
1115	15	0.6827857965496023
1115	16	0.6499108389788333
1115	17	0.67318881000039
1115	18	0.9754868579662812
1115	19	0.2801984738321579
1115	20	0.9665230068140929
1115	21	-0.13975189149892486
1115	22	0.9645523774792437
1115	23	0.8690193566236317
1115	24	0.976431686386718
1115	25	0.9710747030102596
1116	1	0.6991474777185793
1116	2	0.7742541164443999
1116	3	0.5680002381155589
1116	4	0.8271018931289618
1116	5	0.7360758315671276
1116	6	0.6765975186602589
1116	7	0.7056066444015912
1116	8	0.6812054508978065
1116	9	0.9076146723089664
1116	10	0.7988015796001164
1116	11	0.724211695654958
1116	12	0.7984194855572824
1116	13	0.13328773625013923
1116	14	1.319953014256157
1116	15	0.9889035704207458
1116	16	0.9360186008130356
1116	17	1.1124518463576862
1116	18	1.1326896441555974
1116	19	1.2946650490712344
1116	20	1.0994632219655074
1116	21	0.5247124338442077
1116	22	0.4853662159755743
1116	23	-0.4303975623893166
1116	24	0.15762270971097792
1116	25	-0.41396430711733956
1201	1	0.3145911286549463
1201	2	0.5745416204048048
1201	3	0.4223037349743528
1201	4	0.33883361033181814
1201	5	0.3479144440126518
1201	6	0.20742837584878515
1201	7	0.2033438619284098
1201	8	0.24279580663489497
1201	9	0.47193235526538047
1201	10	0.7829317748659008
1201	11	0.4598179443883481
1201	12	0.4895757238722993
1201	13	0.4113784216641463
1201	14	1.0594604398362293
1201	15	0.5924699325287364
1201	16	0.3969993396889322
1201	17	0.3767970737491311
1201	18	0.44941211327871855
1201	19	0.7300633587277758
1201	20	0.7181661787821128
1201	21	0.6063210288496776
1201	22	0.7859587781218167
1201	23	0.3795251419318788
1201	24	0.6354437308245693
1201	25	0.8237452375289038
1202	1	0.24058503649055396
1202	2	0.6026622865043585
1202	3	0.46944342512463777
1202	4	0.41783682397002053
1202	5	0.3636620015616927
1202	6	0.2992327821420204
1202	7	0.2696765336303528
1202	8	0.36507173270403115
1202	9	0.8758252397204842
1202	10	-0.17653397926500247
1202	11	0.41308322420687205
1202	12	0.3110226343973963
1202	13	0.3880932499407237
1202	14	1.0755996917631236
1202	15	0.6574867310920496
1202	16	0.6661840293666259
1202	17	-1.461125403673769
1202	18	0.14826242132398448
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2210	18	0.509846797506718
2210	19	0.646816715393939
2210	20	0.7893134450105279
2210	21	0.8903997492465123
2210	22	1.0374768326610848
2210	23	1.3625018218322396
2210	24	1.0740694151613042
2210	25	0.9655075080820268
2211	1	0.17604487202070374
2211	2	-0.5948676318798789
2211	3	-0.002222458991091969
2211	4	0.35566883109449776
2211	5	0.41731240045628226
2211	6	0.21328045548909103
2211	7	0.3361585939510974
2211	8	0.3482179589682346
2211	9	0.9089536651007251
2211	10	0.9593536140864458
2211	11	0.4824190396885797
2211	12	0.3553045880160326
2211	13	0.3372621395680184
2211	14	-0.8521582740034574
2211	15	0.045983665728236645
2211	16	0.29657654659736465
2211	17	0.2538608354689771
2211	18	0.3077449264088443
2211	19	0.6859181714992104
2211	20	0.803665374036008
2211	21	0.8725976917411558
2211	22	0.15012001403214725
2211	23	0.6935568720154421
2211	24	0.0973606449593376
2211	25	0.45895897333767977
2212	1	-0.001335220070686205
2212	2	-1.046036427225701
2212	3	-0.266127388996825
2212	4	0.27904448739231924
2212	5	0.2421879154286728
2212	6	0.15051532346748786
2212	7	0.2405925705415939
2212	8	0.17380483933220603
2212	9	-2.1373500112478396
2212	10	0.036444735035559006
2212	11	0.12346392995402766
2212	12	0.11445026974302408
2212	13	0.1433977228512262
2212	14	0.6543159890660917
2212	15	0.31189303591337136
2212	16	0.43510894567656067
2212	17	0.4431164142598405
2212	18	0.14182597789194773
2212	19	-0.6104015405274268
2212	20	0.42657181738683647
2212	21	0.45239443687835035
2212	22	-1.6805424423455193
2212	23	-0.33211149073908786
2212	24	0.3552075738195839
2212	25	-0.48329155713194893
2213	1	0.11696788662075264
2213	2	0.38763732220422753
2213	3	0.17676541235258725
2213	4	0.24266483971279484
2213	5	0.35202275772286384
2213	6	0.2048117141985557
2213	7	0.1627948277399227
2213	8	0.22552303644079075
2213	9	0.7315727653147803
2213	10	0.6489243210318634
2213	11	0.08578951004759006
2213	12	0.06423652425944937
2213	13	0.06121568257194099
2213	14	-1.7345248531531539
2213	15	-0.5146442592643723
2213	16	-0.18505406470074348
2213	17	-2.153130394485025
2213	18	-0.9966257873722384
2213	19	-0.53455217569973
2213	20	0.13168429401850545
2213	21	-0.15881237884494936
2213	22	0.3928619845097998
2213	23	0.8584310276869687
2213	24	0.579683091553548
2213	25	-0.8307012429984653
2214	1	0.4056000228982306
2214	2	0.32986921813461834
2214	3	0.3600979477668922
2214	4	0.620390301756089
2214	5	0.6539193919770554
2214	6	0.6515031294609841
2214	7	0.5669270152461152
2214	8	0.4274925704275532
2214	9	0.9039592779082923
2214	10	0.8328848996688691
2214	11	0.331726194132024
2214	12	0.26668816323524425
2214	13	0.4176094925397038
2214	14	1.2493466977647572
2214	15	0.8783631253027622
2214	16	0.7970615247020021
2214	17	0.6605432547826098
2214	18	0.5885395410647192
2214	19	-0.5683909965188079
2214	20	0.5692428128988496
2214	21	0.7938130869836542
2214	22	0.9327139539551297
2214	23	1.4652462892336473
2214	24	1.1338536601213534
2214	25	1.1082086115019578
2215	1	-0.014607178057104875
2215	2	0.4254515444686966
2215	3	0.3247651122982589
2215	4	0.4615860760732886
2215	5	0.5154648498780574
2215	6	0.3677343023566024
2215	7	0.3283984137308025
2215	8	0.3559144240740153
2215	9	0.48467565630404863
2215	10	0.8671488473979778
2215	11	0.44103030364469475
2215	12	0.37095828475211334
2215	13	0.2338341256006018
2215	14	1.14933998945655
2215	15	0.5542182086917791
2215	16	0.559552800455279
2215	17	0.5978191563187423
2215	18	0.5777179525036473
2215	19	-0.2740607575330287
2215	20	-1.4627275979482732
2215	21	-1.6201780546800464
2215	22	0.11990309164193036
2215	23	0.6819821551115908
2215	24	0.8101622435242319
2215	25	0.0991991085248074
2216	1	0.19762304993569302
2216	2	-0.10625814787707455
2216	3	0.021602252173096304
2216	4	0.3228814890075853
2216	5	0.4923062326234131
2216	6	0.2713208346247029
2216	7	0.2755102640989051
2216	8	0.12100797475710579
2216	9	0.4864110057182742
2216	10	-1.4446156640646919
2216	11	-0.30546133436465794
2216	12	-0.11557805712486413
2216	13	0.08958336887850038
2216	14	-0.4177683866769968
2216	15	-0.2625337509456074
2216	16	0.21117008738554688
2216	17	0.48636209924136864
2216	18	0.27572964499391617
2216	19	-1.152164932302398
2216	20	-0.8766083179449495
2216	21	-0.6145637838772942
2216	22	-0.43935366812704757
2216	23	-4.0579315731878935
2216	24	-1.7666091164848723
2216	25	-0.8439236749212355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	17.0
4	42.0
5	59.0
6	80.0
7	253.0
8	174.0
9	426.0
10	1191.0
11	2751.0
12	5643.0
13	9337.0
14	13428.0
15	17226.0
16	19880.0
17	22217.0
18	23169.0
19	24054.0
20	24335.0
21	25187.0
22	26416.0
23	27584.0
24	29892.0
25	32872.0
26	36944.0
27	42522.0
28	49469.0
29	59814.0
30	73112.0
31	89075.0
32	116227.0
33	151754.0
34	201493.0
35	271171.0
36	374070.0
37	535198.0
38	816086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31232349334253	21.690246818514066	14.31395489312585	22.68347479501755
2	15.575075395809987	21.61138387860322	40.39021675114435	22.423323974442447
3	17.680080558941714	27.107866091257854	30.586198259808995	24.625855089991436
4	12.589131305896753	18.397957167004225	39.0363488844593	29.97656264263972
5	13.423242761863879	37.844768488560575	35.323834113900936	13.40815463567461
6	29.559570981457895	39.30315070699798	18.042227409546435	13.095050901997682
7	24.99999199341282	33.477238393571156	24.134608024714094	17.38816158830193
8	22.952324637577174	37.07238462657108	22.02298852047435	17.9523022153774
9	24.14761452505914	17.8727479768027	22.690431994464934	35.28920550367323
10	14.980769249246478	30.434932997431734	33.85653017621703	20.727767577104757
11	30.814129821748665	26.40253985009555	24.320031873030555	18.463298455125226
12	20.6284622878433	28.127403866169388	31.564151882389336	19.67998196359798
13	25.752109673559488	25.024309127548932	27.234582120012146	21.988999078879438
14	20.876687826189738	24.378788029243	28.28074844298605	26.463775701581216
15	22.370121238328608	31.396595076794952	24.743061227758293	21.490222457118147
16	21.49831312305074	31.63683752367617	26.19008576265606	20.674763590617033
17	19.886618032607323	30.660819391448662	28.692888661995426	20.75967391394859
18	19.874621383546277	30.410896338922623	29.545935700300713	20.16854657723039
19	20.61891524902644	29.357898647263784	29.882147981143675	20.141038122566098
20	21.93131117490402	28.93556147369311	29.132960823722765	20.0001665276801
21	22.406876717426105	29.30235954052317	28.0301873113253	20.260576430725425
22	21.38860128185379	28.877034461710117	29.461820606441808	20.272543649994283
23	21.69978638979539	29.05633608859539	28.73496001614086	20.508917505468357
24	21.31029257100986	28.402535897242103	29.164451578274498	21.12271995347354
25	21.79606680671811	28.727779868363612	28.627196691076307	20.848956633841976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3814.0
1	3814.0
2	3968.0
3	4122.0
4	4122.0
5	4122.0
6	5821.5
7	7521.0
8	7521.0
9	7521.0
10	11639.0
11	15757.0
12	15757.0
13	15757.0
14	25657.0
15	35557.0
16	35557.0
17	35557.0
18	54835.0
19	74113.0
20	74113.0
21	74113.0
22	102967.5
23	131822.0
24	131822.0
25	131822.0
26	168111.0
27	204400.0
28	204400.0
29	204400.0
30	240934.0
31	277468.0
32	277468.0
33	277468.0
34	307224.5
35	336981.0
36	336981.0
37	336981.0
38	354772.0
39	372563.0
40	372563.0
41	372563.0
42	370620.0
43	368677.0
44	368677.0
45	368677.0
46	352836.0
47	336995.0
48	336995.0
49	336995.0
50	320412.5
51	303830.0
52	303830.0
53	303830.0
54	274999.0
55	246168.0
56	246168.0
57	246168.0
58	212719.0
59	179270.0
60	179270.0
61	179270.0
62	147086.5
63	114903.0
64	114903.0
65	114903.0
66	88460.5
67	62018.0
68	62018.0
69	62018.0
70	45378.5
71	28739.0
72	28739.0
73	28739.0
74	20310.0
75	11881.0
76	11881.0
77	11881.0
78	8158.5
79	4436.0
80	4436.0
81	4436.0
82	2979.5
83	1523.0
84	1523.0
85	1523.0
86	987.0
87	451.0
88	451.0
89	451.0
90	287.0
91	123.0
92	123.0
93	123.0
94	74.5
95	26.0
96	26.0
97	26.0
98	19.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08123156920692758
2	0.02078016886688845
3	0.03150645017722378
4	0.039415081471709836
5	0.04834831277195926
6	0.019243268858243387
7	0.023725893883458155
8	0.04047170022765331
9	0.04018353147603237
10	0.026479506398947222
11	0.025006643890662372
12	0.01908317510734286
13	0.028368612659573446
14	0.042552918989360165
15	0.03906287521972868
16	0.02663960014984775
17	0.0257430751448048
18	0.03032175642055988
19	0.01953143760986434
20	0.018122612601939696
21	0.012839518822222295
22	0.021164393869049714
23	0.02113237511886961
24	0.020684112616348133
25	0.1077110756058748
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3123170.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.00535949296277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5357755711172	74.31949238761818
2	8.04915805380951	13.3624651574427
3	1.3595410876659506	3.385475901814975
4	0.39656535569044665	1.316681996461607
5	0.19143797363728127	0.7945188911183432
6	0.1209474835196305	0.6023573609589672
7	0.07293907512144937	0.42380339060780853
8	0.04987238279102867	0.3311740049871988
9	0.033421351058836375	0.2496736133441303
>10	0.17355239908687467	2.5006043005414798
>50	0.010150152591366379	0.5899899097086168
>100	0.005632051978575736	0.8886338155079403
>500	5.809972688006585E-4	0.34388272592969893
>1k	4.260646631309932E-4	0.8912465439583828
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4930	0.15785243838791996	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4307	0.13790475702571425	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3619	0.11587585690180169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	3.201875018010547E-5	0.0	0.0	0.0
8	0.0	3.201875018010547E-5	0.0	0.0	0.0
9	0.0	3.201875018010547E-5	0.0	6.403750036021094E-5	0.0
10	0.0	3.201875018010547E-5	0.0	6.403750036021094E-5	0.0
11	0.0	3.201875018010547E-5	0.0	6.403750036021094E-5	0.0
12	0.0	3.201875018010547E-5	0.0	6.403750036021094E-5	0.0
13	0.0	3.201875018010547E-5	0.0	9.605625054031642E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAG	140	3.6379788E-12	12.900471	1
CGTCAGA	160	3.6379788E-12	11.875921	2
GGTATCA	3060	0.0	9.567738	1
AGCACCG	80	0.0045117303	9.501498	5
GCACCGT	80	0.00452128	9.4990635	6
GTGGTAT	1580	0.0	9.144637	1
CGAATGT	85	0.007447218	8.939865	15
GTCCTAG	305	0.0	8.726461	1
TGGTATC	1560	0.0	8.709009	2
GTCTTGG	430	0.0	8.621367	1
TCTACAC	410	0.0	8.342645	3
GGGCGAC	115	0.0011122962	8.259923	11
TATTCCG	140	1.0377115E-4	8.144141	5
TACCCTA	200	2.3016219E-7	8.076274	5
TAGACTG	425	0.0	8.048327	5
GTCTAAG	275	1.9463187E-10	7.950147	1
ATTATAC	615	0.0	7.7246714	3
GTATCAA	5155	0.0	7.670871	1
CTAGGAC	310	4.0017767E-11	7.662376	3
TAGGACC	250	1.7391358E-8	7.601198	4
>>END_MODULE