FastQCFastQC Report
Wed 25 May 2016
SRR1295346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123170
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136260.43628748995411715No Hit
GGTATCAACGCAGAGTACTTTTTTT117860.3773729896227231No Hit
TATCAACGCAGAGTACTTTTTTTTT79870.25573375768850237No Hit
GTGGTATCAACGCAGAGTACATGGG56760.18173842602227866No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54140.173349513475091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC2350.012.1355962
TTAGACG550.003054998812.0986294
AGCGTCA2500.011.0272123
GGTATCA46800.010.7988811
GCAGCGT2700.010.2226691
GTGGTAT20750.09.9534051
ACGGTGA1252.5837926E-69.8764958
GGTTTAG1752.4956535E-99.789591
TGGTATC20450.09.7618872
AAGACCG900.00110769099.5060655
GTAAGAC2700.09.1541363
TAGCGTG850.0073994258.9468855
AAGACGT850.0073994258.9468855
TCTAGTA2800.08.8272022
GTTCTAA3600.08.7245191
TAGAGGT3300.08.6418784
AACACGG1651.0411568E-68.6418785
GTATTAA5400.08.460141
TCTATAC4500.08.4499723
GGTCTAG2159.407813E-98.4109531
Produced by FastQC (version 0.11.5)