FastQCFastQC Report
Wed 25 May 2016
SRR1295345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287369
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG34060.1489047023020772No Hit
GTATCAACGCAGAGTACTTTTTTTT32780.1433087534193215No Hit
GGTATCAACGCAGAGTACTTTTTTT29340.12826964079691558No Hit
ATTCCATTCCATTCCATTCCATTCC23060.10081451659089548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG600.005886215311.0810256
CTAGCAC2450.010.4724743
CGTACAC750.002641918610.1363213
GTCGCCC1606.197297E-910.09164711
TAGCACC1554.0467057E-89.8093434
TCGCCCA1554.078356E-89.80440912
TGTCGCC1752.581146E-99.7698210
GGTATCA22900.09.7579741
TCTAGAC2054.1836756E-119.7345763
ACTTTCG800.00452459379.4980218
GTATAGA3650.09.3785761
TCTGTCG1651.06940206E-79.2102028
GTGGTAT11650.09.0599191
AATACTG3900.09.0154785
GTACGGA850.0073805098.94949151
GTATATA11250.08.8749121
GTAACAC1502.6915404E-68.8692813
GTCTAGA1951.6232661E-88.7773861
TAAGGTG1856.544542E-88.7323045
CGCTCTG1107.1246555E-48.638915
Produced by FastQC (version 0.11.5)