##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295345_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2287369
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.062470462789346	31.0	30.0	33.0	26.0	34.0
2	30.079605433141744	31.0	30.0	34.0	25.0	34.0
3	30.338037282135065	31.0	30.0	34.0	26.0	34.0
4	33.466323098721716	35.0	33.0	37.0	27.0	37.0
5	33.8760252499706	35.0	35.0	37.0	28.0	37.0
6	34.05938044976565	37.0	35.0	37.0	29.0	37.0
7	33.96751595391911	37.0	35.0	37.0	28.0	37.0
8	33.923295279423655	37.0	35.0	37.0	28.0	37.0
9	34.88626889671059	38.0	35.0	39.0	27.0	39.0
10	34.80861067890664	38.0	34.0	39.0	27.0	39.0
11	35.23821998112242	39.0	35.0	39.0	27.0	39.0
12	35.23220608480748	39.0	35.0	39.0	27.0	39.0
13	35.14981622991306	38.0	35.0	39.0	27.0	39.0
14	35.33375594405625	39.0	34.0	41.0	24.0	41.0
15	35.958574239661374	39.0	35.0	41.0	27.0	41.0
16	36.03983091490704	39.0	35.0	41.0	27.0	41.0
17	35.86438043009239	39.0	35.0	41.0	27.0	41.0
18	35.84084553038885	39.0	34.0	41.0	27.0	41.0
19	35.91505305877626	39.0	34.0	41.0	27.0	41.0
20	35.8944857607146	39.0	34.0	41.0	27.0	41.0
21	35.89413907419397	39.0	34.0	41.0	27.0	41.0
22	35.63468815044708	39.0	34.0	41.0	25.0	41.0
23	35.31982378007221	39.0	34.0	41.0	25.0	41.0
24	35.642768613197084	39.0	34.0	41.0	26.0	41.0
25	34.32244032335841	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26073154575568225
1101	2	0.5137589282610442
1101	3	0.23780813109734922
1101	4	0.2091937537557058
1101	5	0.18075078665467714
1101	6	0.1311485011723832
1101	7	0.08973490565847442
1101	8	0.1263858876014794
1101	9	0.09675395111642615
1101	10	0.49683894099295145
1101	11	0.34545233424503863
1101	12	0.2767360669898693
1101	13	0.2827735163871665
1101	14	0.6839884444308453
1101	15	0.4372081495451212
1101	16	0.17180958753200315
1101	17	0.570923571553962
1101	18	0.6555673975880509
1101	19	0.5640756781367173
1101	20	0.5640418848293649
1101	21	0.5952760725164978
1101	22	0.4894344253091276
1101	23	-1.7979426398861165
1101	24	-0.03735651036998888
1101	25	0.2928531781766708
1102	1	0.18784408330459712
1102	2	0.37837229272183137
1102	3	0.25984787550511257
1102	4	0.3116525095254161
1102	5	0.09019458261052904
1102	6	0.15303154133433594
1102	7	0.1072798772162642
1102	8	0.1300442879946928
1102	9	0.23452237738042925
1102	10	0.8320657424693678
1102	11	0.5271747721163891
1102	12	0.4459095747962678
1102	13	0.3295888920398937
1102	14	0.8789674355343422
1102	15	0.6219697108400908
1102	16	0.32314228494342956
1102	17	0.7416065047330846
1102	18	0.7193126646290153
1102	19	0.8089365542151725
1102	20	0.7821032761895026
1102	21	0.7411479506129766
1102	22	0.6580722364858502
1102	23	0.7017775260226387
1102	24	0.7617043311382119
1102	25	0.7049878342844735
1103	1	-0.5545654443916739
1103	2	-1.6257723806817772
1103	3	-0.5110797709762522
1103	4	-0.2900442037148636
1103	5	-0.32390909375914845
1103	6	-0.298943490474997
1103	7	-0.19422031933046213
1103	8	-0.10347679325552406
1103	9	0.4267952983470238
1103	10	0.5850932977494452
1103	11	0.2536769281243352
1103	12	0.04176424073140339
1103	13	-0.0333317051858657
1103	14	-1.2647239101335401
1103	15	-0.4294906958809932
1103	16	-0.31890213731881545
1103	17	0.10211043311500134
1103	18	0.2384806248403848
1103	19	0.12739382309559488
1103	20	0.20588278710197727
1103	21	0.36889701465116786
1103	22	-0.13586684213794342
1103	23	0.633689476694066
1103	24	0.4344443516247125
1103	25	0.2886560778294367
1104	1	-0.05875414726640571
1104	2	0.0921753060240249
1104	3	-0.32517888818468776
1104	4	-0.3782042785920652
1104	5	-0.30200537316468257
1104	6	-0.10964638976258811
1104	7	-0.08299514656417273
1104	8	-0.060138908649648215
1104	9	-2.191003666582155
1104	10	-0.598281376921662
1104	11	-0.42490804902432444
1104	12	-0.07965753504564788
1104	13	0.1434150279879347
1104	14	-1.8668708840200239
1104	15	-0.6682187406083457
1104	16	-0.25686022258575747
1104	17	-0.00883651596353019
1104	18	0.30875142818039336
1104	19	0.3541108685819765
1104	20	0.4893430617965109
1104	21	0.638198497681806
1104	22	0.6429903456037778
1104	23	1.0028091187040005
1104	24	0.6911530448910881
1104	25	0.5189143166349268
1105	1	-0.1923897678212647
1105	2	0.03829568433755881
1105	3	-1.2137416724070675
1105	4	-0.5698416412091305
1105	5	-0.37748009395010484
1105	6	-0.06797300787732041
1105	7	-0.2136322262788184
1105	8	-0.10504105596977809
1105	9	0.3208965037936835
1105	10	0.4388927081242784
1105	11	-0.27919178032018266
1105	12	-0.13806447881857054
1105	13	-0.24327444111190033
1105	14	0.5049150156189199
1105	15	0.23578491086454534
1105	16	-0.1944900752396066
1105	17	0.23121032554695375
1105	18	0.03240008730080035
1105	19	0.22766440648034347
1105	20	-0.38966054350296986
1105	21	0.06940699532218986
1105	22	0.13062932453080123
1105	23	0.2726699247695308
1105	24	0.2545721314655722
1105	25	0.40369040012992485
1106	1	-1.6372416826091296
1106	2	-1.1821312477577095
1106	3	-1.3518773463146054
1106	4	-1.4905664704346755
1106	5	-1.0217778490709222
1106	6	-0.8505589357074683
1106	7	-0.6147395773665636
1106	8	-0.7921982362427258
1106	9	-0.35270280681491073
1106	10	-1.9897297839159265
1106	11	-1.2811113287300842
1106	12	-0.8051983243542224
1106	13	-1.0184411812039187
1106	14	-0.32092812524317793
1106	15	-1.1326102369301978
1106	16	-1.5151006513946044
1106	17	-0.8826433433805718
1106	18	-0.9132675750458716
1106	19	-0.8116738294070629
1106	20	-0.5337170439056038
1106	21	-0.42316252073214855
1106	22	-0.37149194187089307
1106	23	-0.7375595980302521
1106	24	-0.9438869044515243
1106	25	-1.734465445576788
1107	1	-0.17397583544683215
1107	2	-0.07537401116050901
1107	3	-0.19805921969115303
1107	4	-0.08066833842661936
1107	5	-0.28378452900680173
1107	6	-0.11744644685099814
1107	7	-0.19370512823805797
1107	8	-0.3589625676104049
1107	9	0.2883546917324864
1107	10	-0.12172376469342794
1107	11	0.004844837538918512
1107	12	-0.18662070564216293
1107	13	-0.17295247308400974
1107	14	-1.3239454631314445
1107	15	-0.6069875042691137
1107	16	-0.4279900224256892
1107	17	-0.2844939246297713
1107	18	0.13034654773692722
1107	19	0.027442463015610485
1107	20	0.13610655523780224
1107	21	0.17144895644989333
1107	22	-0.5205528634905008
1107	23	0.2364881711349014
1107	24	0.053363442732063504
1107	25	-0.17785374449395874
1108	1	-0.0225746919443246
1108	2	0.24506080213800274
1108	3	-0.0016338414647592003
1108	4	-0.27494317901859944
1108	5	-0.21075330621204102
1108	6	-0.22388629532515125
1108	7	-0.06834101915827517
1108	8	-0.12683920580715125
1108	9	0.3054801711329702
1108	10	0.36127663529715903
1108	11	0.07228913296726347
1108	12	-0.05056920097168671
1108	13	-0.09909950491855568
1108	14	0.0502016157608125
1108	15	0.022821794335627033
1108	16	-0.0964307649964482
1108	17	0.20729803320651286
1108	18	0.188640470232869
1108	19	0.27167671632729906
1108	20	0.35950835754833577
1108	21	0.301549424674171
1108	22	0.24478795201972048
1108	23	0.04962374441552697
1108	24	0.1382132109728147
1108	25	-0.5344841767190829
1109	1	-0.6830519169624303
1109	2	-0.6047964245301323
1109	3	-0.7005423015294738
1109	4	-0.601345245221431
1109	5	-0.9240926438171186
1109	6	-1.1738988997123911
1109	7	-0.906330183968656
1109	8	-1.289057898800948
1109	9	-0.859973265968847
1109	10	-1.0749612071984131
1109	11	-1.186590543260408
1109	12	-1.0694899904188375
1109	13	-0.9981044458383579
1109	14	-0.5360765607739424
1109	15	-1.1553455735933156
1109	16	-1.2445210857437274
1109	17	-1.2889822975326055
1109	18	-1.8472345950807778
1109	19	-2.1370198525293205
1109	20	-1.6220566948400759
1109	21	-1.4305349284686812
1109	22	-1.4671742989863432
1109	23	-1.1104650363048023
1109	24	-1.944659947640794
1109	25	-1.0140148810064105
1110	1	0.11744250767658215
1110	2	0.2401878687251049
1110	3	0.1472636862359913
1110	4	0.45757785249063687
1110	5	0.3388076354379521
1110	6	0.3279462833309381
1110	7	0.35892974031614244
1110	8	0.14209997539728647
1110	9	0.5002108859117698
1110	10	0.4058844956409189
1110	11	0.3060632797139604
1110	12	0.4200434331444072
1110	13	0.30352782492805375
1110	14	-2.2911289562728356
1110	15	-0.5346015386466973
1110	16	0.001535994103555538
1110	17	-0.11104910662523793
1110	18	-2.3451997839003056
1110	19	-0.1008430866375889
1110	20	-1.5296430108160592
1110	21	0.006797649135073414
1110	22	-1.5624914657672662
1110	23	-0.2870883783921059
1110	24	0.2832703675361614
1110	25	0.27189928122374596
1111	1	-0.18691111031075636
1111	2	-1.3973846107472525
1111	3	-0.39177596264964976
1111	4	0.07349408698313198
1111	5	-0.03323885447932895
1111	6	0.0933696725665456
1111	7	-0.03599010413146431
1111	8	-0.06018526864598073
1111	9	-1.854928071596234
1111	10	-0.32323080771958246
1111	11	-0.12674866617611968
1111	12	-0.08021996324442426
1111	13	-0.12331102437223507
1111	14	0.8215167983168996
1111	15	0.2280543698889872
1111	16	0.3246021220553956
1111	17	-0.0862555954513482
1111	18	0.09303664239568832
1111	19	-0.23653905627880079
1111	20	0.28122489392477235
1111	21	-0.680876369796735
1111	22	-0.31549748842117253
1111	23	0.03292338199901934
1111	24	-0.5297159231212945
1111	25	-1.8458381468272123
1112	1	0.26549491615279663
1112	2	0.3729748372100481
1112	3	0.21895386396339944
1112	4	0.6539386277665216
1112	5	0.37878545378950434
1112	6	0.36999656856445995
1112	7	0.33271608532831465
1112	8	0.335571275309384
1112	9	-0.02395840216308187
1112	10	0.7767536871965248
1112	11	0.42179921714851076
1112	12	0.3791591082864656
1112	13	0.32989478671721884
1112	14	0.9025360300207055
1112	15	0.49452057613437006
1112	16	0.4892462880940158
1112	17	-3.195685309208983
1112	18	-0.678849323497758
1112	19	-0.9197190487036835
1112	20	0.25703279750629093
1112	21	-0.23845662017684077
1112	22	-1.4239385795162391
1112	23	-1.2207254425073444
1112	24	-0.09086535124597361
1112	25	0.2960018977904042
1113	1	-0.2373349049308331
1113	2	0.032562241834646954
1113	3	0.013131318171758721
1113	4	-0.3145174865614351
1113	5	-0.1391777169700461
1113	6	-0.30913767374239853
1113	7	-0.2575239507054974
1113	8	-0.2781274758972003
1113	9	0.2642987929815064
1113	10	0.4567255696263217
1113	11	-0.09085943191359291
1113	12	0.04756687177212626
1113	13	-0.2615012733090225
1113	14	-1.4570751278964025
1113	15	-1.6698323565682074
1113	16	-0.4158925842414263
1113	17	-0.29999443636789636
1113	18	-0.6267720360796503
1113	19	-0.05442151380562166
1113	20	0.30150659742690067
1113	21	-1.6253256510021927
1113	22	-2.1855831500939047
1113	23	-0.6893562268200668
1113	24	-2.360424909831764
1113	25	-0.60025668102341
1114	1	0.16827797580560144
1114	2	0.11592621151264026
1114	3	-0.17246550995165677
1114	4	0.30400494880608875
1114	5	-0.025856127127838135
1114	6	0.09822841481398825
1114	7	0.15224624987202873
1114	8	0.03375920659528475
1114	9	0.4143384860419985
1114	10	0.3422285722904945
1114	11	0.18167642508343818
1114	12	0.04047481312845491
1114	13	0.12635311133212213
1114	14	1.00179676062465
1114	15	0.3462042295398007
1114	16	0.26781832266770067
1114	17	-0.2472989407686086
1114	18	-0.2118019349596807
1114	19	-0.4681908062924123
1114	20	-1.4971607952890054
1114	21	-0.7662204802298049
1114	22	-1.4243537863463374
1114	23	-2.696826581006455
1114	24	-1.9249075547677563
1114	25	-1.397750783007318
1115	1	0.48599596536435286
1115	2	0.48752154807762693
1115	3	0.4410496065939107
1115	4	0.7911014963761858
1115	5	0.7026301913359845
1115	6	0.5368112796962237
1115	7	0.6746562364317725
1115	8	0.7016745387377696
1115	9	0.919154981048834
1115	10	1.1806382225558991
1115	11	0.8845546208873571
1115	12	0.7174809182526829
1115	13	0.5880175149221785
1115	14	1.3299739647057933
1115	15	0.9610815684841825
1115	16	0.872657506025277
1115	17	0.7948728296252838
1115	18	1.1358791889083548
1115	19	0.45783319268488043
1115	20	1.0128286436868166
1115	21	0.11858051299017802
1115	22	1.0258042835084495
1115	23	1.1806636958259773
1115	24	1.1192520376250812
1115	25	1.2083702345824818
1116	1	0.6306209169799324
1116	2	0.7186770448550703
1116	3	0.6855067487488746
1116	4	1.113684195335999
1116	5	0.8058689523261648
1116	6	0.6255281094875045
1116	7	0.6277753419804668
1116	8	0.7548350398467747
1116	9	0.9975672347490914
1116	10	0.8653947490767067
1116	11	0.8772746855227993
1116	12	0.8618603381311516
1116	13	0.4405800460264473
1116	14	1.4694025062918143
1116	15	1.0230485718449813
1116	16	1.0066120799658904
1116	17	1.242130584956783
1116	18	1.2737744051483162
1116	19	1.4636465424842768
1116	20	1.174475081057409
1116	21	0.6991223724265225
1116	22	0.7422774124905658
1116	23	-0.10800497994618041
1116	24	0.3993531380250559
1116	25	-0.17806039290969977
1201	1	0.2995596477303373
1201	2	0.4999529306188393
1201	3	0.45621182988897857
1201	4	0.24663112481376714
1201	5	0.2792642702974675
1201	6	0.1324360785375447
1201	7	0.2893978145886251
1201	8	0.2329674525882126
1201	9	0.3812624981249755
1201	10	0.854918074503118
1201	11	0.3975338255506671
1201	12	0.42396381529520255
1201	13	0.41929724170399396
1201	14	1.018038326541074
1201	15	0.6669440027723539
1201	16	0.46034480710169845
1201	17	0.5218003767411119
1201	18	0.5026551517878701
1201	19	0.719819120518089
1201	20	0.609317083768353
1201	21	0.6013271335244568
1201	22	0.75227520043439
1201	23	0.40023375268683026
1201	24	0.6238780165972955
1201	25	0.8782587926771086
1202	1	0.2997588254203478
1202	2	0.6681757954340029
1202	3	0.3775340301690022
1202	4	0.31399106044998604
1202	5	0.11252380992925737
1202	6	0.09515328017590008
1202	7	0.21640278292916548
1202	8	0.22352381791536402
1202	9	0.7031819702583846
1202	10	-0.18933855517527576
1202	11	0.2643120317237333
1202	12	0.4131814453288456
1202	13	0.2621675946599282
1202	14	1.0573481660495716
1202	15	0.6457599860962802
1202	16	0.42800618674352364
1202	17	-1.7360753050343192
1202	18	-0.05544138865891313
1202	19	0.4381400721825628
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2210	18	0.7238258204378241
2210	19	0.7424810886911075
2210	20	0.8710600714436652
2210	21	0.954947511764999
2210	22	1.1410215408036564
2210	23	1.4221791415521068
2210	24	1.1066650615045077
2210	25	1.161890503967662
2211	1	0.1328930544209932
2211	2	-0.5288219932655984
2211	3	-0.03410845112238903
2211	4	0.2886135214034127
2211	5	0.2340713904180376
2211	6	0.2302745756396405
2211	7	0.23217668098909172
2211	8	0.3244542324246922
2211	9	0.7496050275324606
2211	10	0.8225591318345096
2211	11	0.41202439121192214
2211	12	0.41285113433735177
2211	13	0.2246289107063646
2211	14	-0.7267812525874149
2211	15	-0.052144908065052675
2211	16	0.25287973105656647
2211	17	0.42233459411823304
2211	18	0.46770750250865234
2211	19	0.6431890327475216
2211	20	0.7176010769654937
2211	21	0.5581404297448529
2211	22	0.020129566192828463
2211	23	0.45675747820602197
2211	24	-0.11543726907373042
2211	25	0.37035578807848424
2212	1	0.10938739469967373
2212	2	-1.051889128271963
2212	3	-0.2952132064042985
2212	4	0.26580296123027836
2212	5	0.24395840899147458
2212	6	0.16993178986245283
2212	7	0.2015285509103748
2212	8	0.1439516247513879
2212	9	-2.155655409048258
2212	10	-0.026435291319117482
2212	11	0.09375354808797454
2212	12	-0.0015499213019580793
2212	13	0.06657851343911858
2212	14	0.7516662364217126
2212	15	0.37196586626294703
2212	16	0.4047192323608897
2212	17	0.43412293645580746
2212	18	0.04248950390034878
2212	19	-0.5584595861808808
2212	20	0.25978524954577864
2212	21	0.34966374025470515
2212	22	-1.7670438221444655
2212	23	-0.3572311677013573
2212	24	0.29881438666269133
2212	25	-0.34492429698946125
2213	1	0.3135408054614963
2213	2	0.6057386726545815
2213	3	0.28292026034741014
2213	4	0.5514990346816759
2213	5	0.4759280232112246
2213	6	0.28587722072868615
2213	7	0.32596832740913584
2213	8	0.3691300400008046
2213	9	0.9029077972702737
2213	10	0.8600171421021869
2213	11	0.22278052329735942
2213	12	0.2815347166161004
2213	13	0.3511892385959925
2213	14	-1.1652085654558206
2213	15	0.01706346664181524
2213	16	0.33168901844591403
2213	17	-1.8430286830879439
2213	18	-0.6633908855081643
2213	19	-0.1699730317740631
2213	20	0.48301463621590557
2213	21	0.11105615450347983
2213	22	0.5845586114677985
2213	23	0.9322092771623502
2213	24	0.803892585361865
2213	25	-0.5896112539429339
2214	1	0.4465020600022811
2214	2	0.28446569646503406
2214	3	0.29826419338878196
2214	4	0.8281806622865489
2214	5	0.759890001166525
2214	6	0.6262156698213133
2214	7	0.5447661369410994
2214	8	0.5257099228543254
2214	9	0.908112725823365
2214	10	0.9337645848707581
2214	11	0.21123054935583951
2214	12	0.1912179699589771
2214	13	0.3822130646031354
2214	14	1.1315883417526535
2214	15	0.8384317313026273
2214	16	0.8854657867276856
2214	17	0.7814823120348393
2214	18	0.6740965325243025
2214	19	-0.38976191919364567
2214	20	0.49619727115604917
2214	21	0.8190503516300467
2214	22	1.1130675219121926
2214	23	1.458402016569778
2214	24	1.2495776900236066
2214	25	1.4759626160135824
2215	1	-0.03334983211762932
2215	2	0.27083630639781475
2215	3	0.2676633502146508
2215	4	0.6522546898617065
2215	5	0.6269284336360101
2215	6	0.49652742830892294
2215	7	0.429850077713013
2215	8	0.330689496345812
2215	9	0.5166315340301253
2215	10	0.7819634238114475
2215	11	0.48606313233617726
2215	12	0.37603581371313766
2215	13	0.24379898776330577
2215	14	1.0578413642983975
2215	15	0.5816951893209392
2215	16	0.5950204838202708
2215	17	0.7707922927759228
2215	18	0.834355172046017
2215	19	-0.13817971222679404
2215	20	-1.5609435951689576
2215	21	-1.7390911248537293
2215	22	-0.09839392132671776
2215	23	0.6334231084777642
2215	24	0.8500982081814783
2215	25	0.22682010251099172
2216	1	0.11942516500439027
2216	2	-0.22310492277437888
2216	3	-0.04223239187841443
2216	4	0.4813540577074278
2216	5	0.4721031895083385
2216	6	0.23398569761765486
2216	7	0.3249296100289598
2216	8	0.14093372412155958
2216	9	0.5637759535042832
2216	10	-1.3638443622471286
2216	11	-0.19323431552235348
2216	12	0.17068702771963729
2216	13	0.14819503767496656
2216	14	-0.34809190163183956
2216	15	-0.3316044508065943
2216	16	0.18036631675735748
2216	17	0.4160050880189985
2216	18	0.31268023848979
2216	19	-0.9854216075884921
2216	20	-0.6921337519812596
2216	21	-0.6115278468610086
2216	22	-0.45758193091664623
2216	23	-4.3661720975604865
2216	24	-1.7763845765006607
2216	25	-0.8764405222356189
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	18.0
4	24.0
5	45.0
6	51.0
7	184.0
8	164.0
9	415.0
10	1129.0
11	2403.0
12	4483.0
13	6836.0
14	9818.0
15	12649.0
16	14480.0
17	15759.0
18	16711.0
19	17155.0
20	17128.0
21	17564.0
22	18064.0
23	19320.0
24	20442.0
25	22114.0
26	24850.0
27	28223.0
28	32798.0
29	39766.0
30	48727.0
31	59987.0
32	78523.0
33	102875.0
34	138010.0
35	188704.0
36	266776.0
37	393988.0
38	667184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.271294195272596	22.08978237413377	14.063554208696457	22.575369221897176
2	15.278931072133043	21.639300248761767	41.20918595127873	21.87258272782647
3	17.284613615153642	27.658224360617112	30.91419880017038	24.142963224058867
4	12.646551622340182	18.283029686229625	39.139796044664266	29.930622646765926
5	13.009823480037458	37.9168221245468	35.82440644249531	13.248947952920428
6	29.208186831230016	40.06080611611756	18.085993473377755	12.645013579274671
7	24.560440713719743	33.74199111785891	24.652926576282688	17.044641592138657
8	23.024295439258776	37.66243535167029	21.83134625089981	17.48192295817112
9	24.03553563665975	17.649826861295736	22.92498139163459	35.38965611040993
10	14.743762419187819	30.64207201029918	34.13772354538076	20.47644202513224
11	30.96027923565275	26.503554727231858	24.473831277926408	18.062334759188985
12	20.277215652596727	28.315236497772585	32.08933621809952	19.31821163153117
13	25.410668369221078	25.331210970980777	27.658040196610052	21.600080463188092
14	20.611272927654884	24.23679445800205	28.801857828859323	26.350074785483745
15	22.273307935769324	31.70970855486228	24.7234395957965	21.293543913571895
16	21.48134316773242	31.897500571758915	26.341796822900793	20.279359437607873
17	19.64663037698357	31.07831784724749	29.082152052839273	20.19289972292967
18	19.619006304561438	30.750490760496195	29.973932516660057	19.65657041828231
19	20.459453774990347	29.525394928491565	30.062620986272883	19.952530310245205
20	21.714809668186266	29.16385002925222	29.30110371376376	19.820236588797755
21	22.184556862402168	29.633903196187312	28.27209129465262	19.9094486467579
22	21.06485884481128	29.111729984595208	29.8957870107405	19.92762415985301
23	21.38449180627504	29.42718479290823	29.12848780440879	20.05983559640794
24	20.970365537759758	28.58021139238071	29.39724486779668	21.05217820206285
25	21.668418744578553	28.879043601793686	28.860093150440324	20.59244450318744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2485.0
1	2485.0
2	2739.5
3	2994.0
4	2994.0
5	2994.0
6	4302.0
7	5610.0
8	5610.0
9	5610.0
10	8961.0
11	12312.0
12	12312.0
13	12312.0
14	20148.5
15	27985.0
16	27985.0
17	27985.0
18	42695.0
19	57405.0
20	57405.0
21	57405.0
22	79478.5
23	101552.0
24	101552.0
25	101552.0
26	128352.0
27	155152.0
28	155152.0
29	155152.0
30	182683.5
31	210215.0
32	210215.0
33	210215.0
34	232636.5
35	255058.0
36	255058.0
37	255058.0
38	268534.5
39	282011.0
40	282011.0
41	282011.0
42	279131.0
43	276251.0
44	276251.0
45	276251.0
46	260170.0
47	244089.0
48	244089.0
49	244089.0
50	228442.0
51	212795.0
52	212795.0
53	212795.0
54	190516.5
55	168238.0
56	168238.0
57	168238.0
58	144299.5
59	120361.0
60	120361.0
61	120361.0
62	98946.0
63	77531.0
64	77531.0
65	77531.0
66	59672.0
67	41813.0
68	41813.0
69	41813.0
70	30854.0
71	19895.0
72	19895.0
73	19895.0
74	14169.0
75	8443.0
76	8443.0
77	8443.0
78	5905.5
79	3368.0
80	3368.0
81	3368.0
82	2322.5
83	1277.0
84	1277.0
85	1277.0
86	842.5
87	408.0
88	408.0
89	408.0
90	252.5
91	97.0
92	97.0
93	97.0
94	58.0
95	19.0
96	19.0
97	19.0
98	12.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08249652766999989
2	0.019323510985765742
3	0.031083747309681997
4	0.0388218953741176
5	0.04861480591894005
6	0.01871145407671434
7	0.023301880894599867
8	0.03353197494588761
9	0.033357101543301494
10	0.025575235128219364
11	0.0242636846088235
12	0.018055678817016408
13	0.026624475543736058
14	0.0362425126859724
15	0.03488724381593
16	0.024744586465935317
17	0.025575235128219364
18	0.026755630595675643
19	0.019061200881886568
20	0.015869761284689966
21	0.011760236323916255
22	0.01827427057024905
23	0.019061200881886568
24	0.0191049192325331
25	0.10767829764240051
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2287369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.4949099392787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56824522470505	75.61987478395257
2	7.282387455140604	12.160845694197954
3	1.1291623710146268	2.8283793142406597
4	0.3465020459224517	1.1572462847228366
5	0.16851943545300607	0.7035257543083416
6	0.11258440239994567	0.5640134723370585
7	0.078225567692986	0.457200571063237
8	0.05269121099160918	0.3519558333068752
9	0.039640409522355084	0.29787951807226465
>10	0.20295899417079138	2.9635752945798233
>50	0.011717439392790059	0.6799158485084305
>100	0.006263037365067669	0.9613168896444767
>500	4.7245981232933373E-4	0.2384411808587073
>1k	6.299464164315906E-4	1.015829560206725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	3406	0.1489047023020772	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3278	0.1433087534193215	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2934	0.12826964079691558	No Hit
ATTCCATTCCATTCCATTCCATTCC	2306	0.10081451659089548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.3718350646528825E-5	0.0
5	0.0	0.0	0.0	4.3718350646528825E-5	0.0
6	0.0	0.0	0.0	4.3718350646528825E-5	0.0
7	0.0	0.0	0.0	8.743670129305765E-5	0.0
8	0.0	0.0	0.0	8.743670129305765E-5	0.0
9	0.0	0.0	0.0	1.311550519395865E-4	0.0
10	0.0	0.0	0.0	1.311550519395865E-4	0.0
11	0.0	0.0	0.0	1.311550519395865E-4	0.0
12	0.0	0.0	0.0	1.311550519395865E-4	0.0
13	0.0	0.0	0.0	2.1859175323264414E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGG	60	0.0058862153	11.081025	6
CTAGCAC	245	0.0	10.472474	3
CGTACAC	75	0.0026419186	10.136321	3
GTCGCCC	160	6.197297E-9	10.091647	11
TAGCACC	155	4.0467057E-8	9.809343	4
TCGCCCA	155	4.078356E-8	9.804409	12
TGTCGCC	175	2.581146E-9	9.76982	10
GGTATCA	2290	0.0	9.757974	1
TCTAGAC	205	4.1836756E-11	9.734576	3
ACTTTCG	80	0.0045245937	9.498021	8
GTATAGA	365	0.0	9.378576	1
TCTGTCG	165	1.06940206E-7	9.210202	8
GTGGTAT	1165	0.0	9.059919	1
AATACTG	390	0.0	9.015478	5
GTACGGA	85	0.007380509	8.9494915	1
GTATATA	1125	0.0	8.874912	1
GTAACAC	150	2.6915404E-6	8.869281	3
GTCTAGA	195	1.6232661E-8	8.777386	1
TAAGGTG	185	6.544542E-8	8.732304	5
CGCTCTG	110	7.1246555E-4	8.63891	5
>>END_MODULE