FastQCFastQC Report
Wed 25 May 2016
SRR1295345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287369
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89030.3892244758060462No Hit
GGTATCAACGCAGAGTACTTTTTTT80580.3522824695097293No Hit
TATCAACGCAGAGTACTTTTTTTTT55010.2404946469065551No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37340.16324432131413863No Hit
GTGGTATCAACGCAGAGTACATGGG37170.16250110935314765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1950.011.2154293
GGCGCAT600.005895293311.07873110
GACTCGG700.001481015810.8666495
GGTATCA31300.010.5534121
GCAGCGT1951.4551915E-1110.2516191
CTAGACT1909.822543E-1110.0089744
GACCTCG1356.480295E-79.8479756
CAGCGTC2251.8189894E-129.7200382
TCTAGAC2251.8189894E-129.7200383
GGACAGT2252.7284841E-119.2852346
GTATTAG3900.09.2752741
TATACTG7500.09.2547625
CCTCGTG1554.287358E-79.189738
ACCTCGT1451.7164239E-69.1686057
TAACACC2106.566552E-109.0557384
CGAACTC1054.512207E-49.04386115
GCGCATG850.00746237868.93746311
GTCTAGT1502.6288653E-68.8847361
CTCGTGA1502.717532E-68.8629849
GTTCAGG2502.5465852E-118.7578121
Produced by FastQC (version 0.11.5)