FastQCFastQC Report
Wed 25 May 2016
SRR1295344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291301
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG39680.17317672361684475No Hit
GTATCAACGCAGAGTACTTTTTTTT36160.15781427232825368No Hit
GGTATCAACGCAGAGTACTTTTTTT31570.13778198499455113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC600.00588937611.08022617
GCGCGAT600.005891290511.07974316
GTCTAGG1951.4551915E-1110.23931
GGTATCA23800.010.2270081
CCTGCGC856.601897E-410.057768
CTTAGAC1752.564775E-99.7735963
GTGGTAT11300.09.6762041
TGGTATC10750.09.4574992
GTTCTAG2054.1654857E-109.2760211
TAAGACT2900.09.1746494
TGGGCGC1151.11191395E-49.08619410
TAGGACA2451.6370905E-118.9205414
TAGACAG3350.08.7931875
GTCTTAG2502.5465852E-118.7472881
TAAGCCT1856.549817E-88.7318564
TTAGACC1651.0464992E-68.6384684
GACTCCG1107.154663E-48.63526257
CTAGGAC2106.35373E-98.5971453
TCTAGAC2451.546141E-108.5325053
GTGTTAG3150.08.4514861
Produced by FastQC (version 0.11.5)