FastQCFastQC Report
Wed 25 May 2016
SRR1295344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291301
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112170.489547204841267No Hit
GGTATCAACGCAGAGTACTTTTTTT95590.4171865678058012No Hit
TATCAACGCAGAGTACTTTTTTTTT65540.2860383685949598No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45300.19770427368556118No Hit
GTGGTATCAACGCAGAGTACATGGG44530.1943437374661819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.011.9368231
GTGGTAT11850.011.4854061
TGGTATC11850.011.3094362
ATACCGT600.00588967711.0801516
AGCGTCA1752.0008883E-1110.8625593
GACTCCG1158.859224E-710.7355787
GCAGCGT1605.275069E-1010.707331
CAGCGTC1856.002665E-1110.27539352
TCTGGAC2151.05501385E-109.2836993
GCCTTAA1451.6643426E-69.1894331
GACTATC950.00182964438.9970247
GTATCAA55500.08.9002671
GTGTAGA3550.08.8473711
ACTATCC1401.0898932E-58.818518
GTCTTAC2303.8926373E-108.6900071
GTCCTAT1554.1039293E-68.5965661
GTCCTAG2002.4596375E-88.5658651
GCCTTAC1451.6504775E-58.5330451
TAGGACA2356.1118044E-108.4935974
TAGACAG2803.6379788E-128.4863755
Produced by FastQC (version 0.11.5)