FastQCFastQC Report
Wed 25 May 2016
SRR1295343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658485
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA708004.2689563065086515No Hit
GCTTATGTACTCTGCGTTGATACCA426572.572046174671462No Hit
CTTATGTACTCTGCGTTGATACCAC285991.7244051046587698No Hit
GAGTACATAAGCAGTGGTATCAACG205651.2399870966574915No Hit
TATGTACTCTGCGTTGATACCACTG192231.1590698740115226No Hit
GCGTTGATACCACTGCTTATGTACT151050.9107709747148753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA135070.8144179778532817No Hit
CCCATGTACTCTGCGTTGATACCAC132120.7966306599094957No Hit
CATAAGCAGTGGTATCAACGCAGAG128460.7745623264605951No Hit
ACTCTGCGTTGATACCACTGCTTAT116470.702267430817885No Hit
CTGCTTATGTACTCTGCGTTGATAC113370.6835756729786522No Hit
ACGCAGAGTACATAAGCAGTGGTAT111360.6714561783796658No Hit
GTATCAACGCAGAGTACATAAGCAG102360.6171897846528609No Hit
CATGTACTCTGCGTTGATACCACTG98250.59240813151762No Hit
GTACATGATAAGCAGTGGTATCAAC92140.5555672797764225No Hit
ATCATGTACTCTGCGTTGATACCAC91690.5528539600900823No Hit
TATCAACGCAGAGTACATAAGCAGT86440.5211985637494461No Hit
GTATCAACGCAGAGTACTTTTTTTT84940.5121541647949786No Hit
GGTATCAACGCAGAGTACATAAGCA83840.5055216055617024No Hit
GTGGTATCAACGCAGAGTACATGGG67200.40518907316014313No Hit
GTGGTATCAACGCAGAGTACATAAG65510.3949990503381098No Hit
AAGCAGTGGTATCAACGCAGAGTAC64990.39186365870056106No Hit
GTACATGGGAAGCAGTGGTATCAAC59070.3561684308269294No Hit
GGTATCAACGCAGAGTACTTTTTTT55010.3316882576568374No Hit
ACATAAGCAGTGGTATCAACGCAGA54320.32752783413778236No Hit
GTACTGGTTCACTATCGGTCAGTCA52010.3135994597479025No Hit
TATCAACGCAGAGTACTTTTTTTTT49300.2972592456368312No Hit
GCAGTGGTATCAACGCAGAGTACAT45570.27476884023672205No Hit
GATACCACTGCTTATGTACTCTGCG45480.274226176299454No Hit
GTACTCTGCGTTGATACCACTGCTT45220.27265848048067964No Hit
CCACTGCTTATGTACTCTGCGTTGA44330.26729213710102895No Hit
ATACAGGGTGACAGCCCCGTACACA38920.23467200487191625No Hit
ATACCACTGCTTATGTACTCTGCGT38710.2334057890182908No Hit
GTTGATACCACTGCTTATGTACTCT37600.22671293379198484No Hit
GAGTACATGATAAGCAGTGGTATCA36510.2201406705517385No Hit
GGATACCACGTGTCCCGCCCTACTC35080.2115183435484795No Hit
CAGTGGTATCAACGCAGAGTACATA31450.18963089807866818No Hit
GCTTATCATGTACTCTGCGTTGATA30410.18336011480357073No Hit
TGATACCACTGCTTATGTACTCTGC29140.17570252368878825No Hit
TACCACTGCTTATGTACTCTGCGTT28300.17063766027428645No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27040.16304036515253378No Hit
GCGTTGATACCACTGCTTATCATGT25760.15532247804472152No Hit
GAGTACATGGGAAGCAGTGGTATCA25490.1536944862329174No Hit
GCAGAGTACATAAGCAGTGGTATCA25410.15321211828867912No Hit
GGTATCAACGCAGAGTACATGGGGG24200.14591630313207535No Hit
GTTAATGATAGTGTGTCGAAACACA24070.14513245522268817No Hit
CCCCATGTACTCTGCGTTGATACCA23730.14308239145967555No Hit
GATAAGCAGTGGTATCAACGCAGAG23310.14054995975242465No Hit
GTATCAACGCAGAGTACATGGGGGT23300.14048966375939487No Hit
GTACATGGGATAAGCAGTGGTATCA22870.13789693605911418No Hit
GGTATCAACGCAGAGTACATGGGAA22840.13771604808002486No Hit
ACCCTGTATCGCGCGCCTTTCCAGA22810.13753516010093547No Hit
ACGCAGAGTACATGATAAGCAGTGG22790.1374145681148759No Hit
GAACAAAAAAAAAAAAAAAAAAAAA22780.13735427212184614No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22200.1338571045261187No Hit
GCTTATCCCATGTACTCTGCGTTGA22000.13265118466552306No Hit
GTATCAACGCAGAGTACATGGGAAG21730.1310231928537189No Hit
TATCAACGCAGAGTACATGATAAGC21010.1266818813555745No Hit
ATCAACGCAGAGTACATAAGCAGTG20960.1263804013904256No Hit
CCCATATTCAGACAGGATACCACGT20690.12475240957862145No Hit
TCTAAGTACCCCGAGGAAAAGAAAT20200.12179790592016207No Hit
GCGTTGATACCACTGCTTCCCATGT20170.12161701794107273No Hit
GTTCACTATCGGTCAGTCAGGAGTA19980.12047139407350685No Hit
GTATTTAGCCTTGGAGGATGGTCCC19530.1177580743871666No Hit
ATCATTAACTGAATCCATAGGTTAA19330.11655215452657094No Hit
ATGTACTCTGCGTTGATACCACTGC19060.11492416271476678No Hit
GTATCAACGCAGAGTACATGATAAG18980.11444179477052853No Hit
CAGTGGTATCAACGCAGAGTACATG18720.11287409895175415No Hit
TATCAACGCAGAGTACATGGGAAGC18300.11034166724450327No Hit
GGTTAATGAGGCGAACCGGGGGAAC18290.11028137125147347No Hit
ACTTAGATGTTTCAGTTCCCCCGGT17980.1084121954675502No Hit
GGTATCAACGCAGAGTACATGATAA17730.10690479564180562No Hit
TATTCAGACAGGATACCACGTGTCC17670.10654301968362694No Hit
CTATCGGTCAGTCAGGAGTATTTAG17020.10262378013669103No Hit
GTACACAAGCAGTGGTATCAACGCA16820.10141786027609535No Hit
ATGATAAGCAGTGGTATCAACGCAG16590.10003105243641035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG453.5316058E-516.8930722
CGTTGTA350.002166693616.289752
CGTGACT350.00217237716.28336113
CGCTTGT350.00217237716.28336114
ATGCCCG456.748432E-414.7818845
TCAGTAC400.00526957714.253963
TCGTATC400.00528436314.24794213
CCGGATA400.00528647814.24708218
CTCTAAC551.948164E-413.8282811
TTCGTTG1650.013.81618513
CCGGTTA500.001501254213.298079513
CGGGTCG500.001501595413.2976778
TCTGATC500.001501595413.2976779
GGGTCGT500.001501595413.2976779
GTCCAGG655.4156648E-513.1634591
GAGCGCC655.4618577E-513.1515489
TACACTG801.9934014E-613.066135
GTACAAA3750.012.9294421
GTCCATG604.0671384E-412.6759241
TCGTACA604.0837404E-412.6698052
Produced by FastQC (version 0.11.5)