##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295343_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1658485
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.742930445557242	31.0	30.0	33.0	25.0	34.0
2	29.60240822196161	31.0	30.0	34.0	23.0	34.0
3	30.060744595217926	31.0	30.0	34.0	25.0	34.0
4	33.18951030609261	35.0	35.0	37.0	25.0	37.0
5	33.8232742533095	35.0	35.0	37.0	28.0	37.0
6	33.81149904883071	37.0	35.0	37.0	28.0	37.0
7	33.93584928413582	37.0	35.0	37.0	28.0	37.0
8	33.809917484933536	37.0	35.0	37.0	28.0	37.0
9	34.66533372324742	39.0	35.0	39.0	25.0	39.0
10	34.6276963614383	38.0	34.0	39.0	25.0	39.0
11	35.04639113407718	39.0	35.0	39.0	27.0	39.0
12	35.074589158177496	39.0	35.0	39.0	27.0	39.0
13	34.80862413588305	38.0	35.0	39.0	26.0	39.0
14	34.997541732364176	39.0	33.0	41.0	21.0	41.0
15	35.53410130329789	39.0	34.0	41.0	25.0	41.0
16	35.549925383708626	39.0	34.0	41.0	26.0	41.0
17	35.595897460634255	39.0	34.0	41.0	26.0	41.0
18	35.52875907831545	39.0	34.0	41.0	25.0	41.0
19	35.61043241271401	39.0	34.0	41.0	25.0	41.0
20	35.63869435056694	39.0	34.0	41.0	25.0	41.0
21	35.688782231976774	39.0	34.0	41.0	25.0	41.0
22	35.45159226643593	39.0	34.0	41.0	25.0	41.0
23	35.13122518443037	39.0	33.0	41.0	24.0	41.0
24	35.48362451273301	39.0	34.0	41.0	25.0	41.0
25	34.345511114058915	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5864253938848414
1101	2	0.6943682162309841
1101	3	0.5655713267032816
1101	4	0.5555186741653699
1101	5	0.46248800958365166
1101	6	0.33212256292225106
1101	7	0.42282374309506565
1101	8	0.42750340591710767
1101	9	0.4178312346370916
1101	10	0.6436566744648147
1101	11	0.6267749725700327
1101	12	0.6774038902816315
1101	13	0.48658073076892805
1101	14	1.0774603547123789
1101	15	0.7598449786370693
1101	16	0.7061788443924968
1101	17	0.7977440575260601
1101	18	0.9209240055706047
1101	19	0.8158055007101837
1101	20	0.9181999091895179
1101	21	0.8096179500477731
1101	22	0.8153888695585394
1101	23	-1.3892581569769504
1101	24	0.20772564214096434
1101	25	0.7344449642268316
1102	1	0.4923746417782162
1102	2	0.6497078110833385
1102	3	0.5675479707544362
1102	4	0.6301748137537899
1102	5	0.48720008433556217
1102	6	0.4115826879916682
1102	7	0.1435503368448181
1102	8	0.34970604412524864
1102	9	0.4801921009480239
1102	10	0.9942357442509788
1102	11	0.6711319596365328
1102	12	0.5356038242861842
1102	13	0.6030457894002481
1102	14	1.2243863351873827
1102	15	0.8522764278809447
1102	16	0.5934658682393845
1102	17	0.8362572284462146
1102	18	0.7777573509762732
1102	19	0.9059206708094152
1102	20	0.9679620841418384
1102	21	1.0085732904474511
1102	22	0.8645329408336195
1102	23	0.8641949339438497
1102	24	0.96621048330924
1102	25	0.7639038820625004
1103	1	-0.22967253977721924
1103	2	-1.4843737855684545
1103	3	-0.48321053415692816
1103	4	-0.035873678725536706
1103	5	-0.21836858324574138
1103	6	-0.1118098626498778
1103	7	0.05329556781572364
1103	8	0.15530572910220997
1103	9	0.7522572993464891
1103	10	0.7518240121509834
1103	11	0.28047764307684275
1103	12	0.30506999902197407
1103	13	0.15591991478911638
1103	14	-1.1045146039457876
1103	15	-0.3683428666112576
1103	16	-0.2080176160424827
1103	17	0.3081818035037145
1103	18	0.4995368298338647
1103	19	0.4249941954139018
1103	20	0.4659207047622971
1103	21	0.5090253418176047
1103	22	0.10482194604470862
1103	23	0.9781187348205336
1103	24	0.7878649729309544
1103	25	0.6632309467049282
1104	1	0.1364278935098966
1104	2	0.16608745834466632
1104	3	-0.1987056984096185
1104	4	0.048351415920777185
1104	5	-0.051414904979161236
1104	6	0.09434322982221488
1104	7	0.08614091218637299
1104	8	0.10786468505856561
1104	9	-1.9711504181149948
1104	10	-0.24394351883952226
1104	11	-0.19095570116224536
1104	12	0.2395029087621907
1104	13	0.31965743592982676
1104	14	-1.5861434380520407
1104	15	-0.22018135074685574
1104	16	-0.12099874563566715
1104	17	0.4033682139026027
1104	18	0.4741243588509434
1104	19	0.5737318117635226
1104	20	0.6296081979462045
1104	21	0.7837684941328291
1104	22	0.7486538798070015
1104	23	1.1846640880196375
1104	24	0.8443584805485358
1104	25	0.6153578272973661
1105	1	-0.08716794855709153
1105	2	-0.0018956019282505565
1105	3	-1.4023100958943253
1105	4	-0.49952127946357905
1105	5	-0.36494957580946874
1105	6	-0.05067708929486514
1105	7	-0.030688450007239965
1105	8	-0.07008115914727142
1105	9	0.24848084338134413
1105	10	0.3426124977579903
1105	11	-0.13929466681224056
1105	12	-0.15753007237311323
1105	13	-0.12024161014110035
1105	14	0.6801225498848211
1105	15	0.29592165648247715
1105	16	-0.11161687456581149
1105	17	0.31231119204351643
1105	18	0.20571181660857008
1105	19	0.26245063796021384
1105	20	-0.35068097517267205
1105	21	0.04544621154737882
1105	22	0.3213732193287555
1105	23	0.37414349085148046
1105	24	0.1836201479994486
1105	25	0.5140325930928071
1106	1	-1.7250017120400152
1106	2	-1.1585038922863795
1106	3	-1.3798741293458114
1106	4	-1.5746863662647428
1106	5	-1.1180793315067632
1106	6	-0.7552666979620994
1106	7	-0.7606124542626205
1106	8	-0.6392848019815887
1106	9	-0.2633560782423743
1106	10	-1.932519385629334
1106	11	-1.2292782082234837
1106	12	-0.9318239512092745
1106	13	-1.023639139921606
1106	14	-0.005549890804630309
1106	15	-0.882896092077992
1106	16	-1.2289428548059647
1106	17	-0.6656274293469266
1106	18	-0.6441787934087486
1106	19	-0.5875714499429634
1106	20	-0.4056807127893691
1106	21	-0.37895085548439766
1106	22	-0.3344636641301264
1106	23	-0.7681787444634054
1106	24	-0.9914482863355545
1106	25	-1.742695248637581
1107	1	0.16191293527986517
1107	2	0.20639848295174446
1107	3	0.05685014597409932
1107	4	0.279958170600608
1107	5	3.9122778142086645E-4
1107	6	0.011229279530262204
1107	7	0.05912934136913606
1107	8	-0.022041052260156846
1107	9	0.42713296780311794
1107	10	0.02748328867419758
1107	11	0.06075490168350939
1107	12	-0.20651424113204087
1107	13	0.09771155005233112
1107	14	-1.2981746834826922
1107	15	-0.6629072676228276
1107	16	-0.23062679633800798
1107	17	-0.157402134131722
1107	18	0.30652687122271516
1107	19	0.28225736926506073
1107	20	0.41804517907092276
1107	21	0.2699303036123837
1107	22	-0.24441337780208272
1107	23	0.5424089971524921
1107	24	0.5065400068826236
1107	25	0.03342695326363554
1108	1	-0.25083022607461203
1108	2	0.1656829786163314
1108	3	-0.2163560864333256
1108	4	-0.43421074015611083
1108	5	-0.492621085060307
1108	6	-0.2809518250670635
1108	7	-0.3122931514661289
1108	8	-0.3656558880415304
1108	9	0.02530307901981388
1108	10	0.1572603850819334
1108	11	-0.1143294004446389
1108	12	-0.10084386724216188
1108	13	-0.3403498231740443
1108	14	0.19700867400883482
1108	15	-0.15770691909470003
1108	16	-0.18881938425225542
1108	17	0.14042716951441747
1108	18	0.12568552756666662
1108	19	-0.001454048978565936
1108	20	0.06338733048329459
1108	21	-0.08601856190068702
1108	22	-0.07661949004741331
1108	23	-0.014238433903777548
1108	24	0.1206458180261194
1108	25	-0.7369412477820632
1109	1	-0.7335270397326532
1109	2	-0.4185500694217801
1109	3	-0.5732660699996934
1109	4	-0.5233178743030749
1109	5	-0.7306383545242809
1109	6	-0.8676405838973551
1109	7	-0.7026807117674352
1109	8	-1.0208374799659836
1109	9	-0.5398072804304874
1109	10	-0.5410268198699555
1109	11	-0.759234703828497
1109	12	-0.7363936230862222
1109	13	-0.8448773517642891
1109	14	-0.2765809934585377
1109	15	-0.8710454392489311
1109	16	-0.9998220886654892
1109	17	-0.955832719117474
1109	18	-1.4871489034497216
1109	19	-1.8730203143290183
1109	20	-1.507095423236855
1109	21	-1.2120733643568826
1109	22	-1.2492906445988226
1109	23	-0.9557271197492412
1109	24	-1.8430258610285009
1109	25	-0.945977456569274
1110	1	0.15502650916272742
1110	2	0.21640514983737447
1110	3	0.04647152794976961
1110	4	0.1216622772753837
1110	5	0.04517460585837796
1110	6	-0.024044711030072108
1110	7	-0.07220715311671455
1110	8	9.539409490670892E-4
1110	9	0.2236886599232264
1110	10	0.10373096722082664
1110	11	0.08269223987323926
1110	12	-6.230075968574056E-4
1110	13	0.0827038546108767
1110	14	-2.7691685449924037
1110	15	-0.7372444829620832
1110	16	-0.2079420985403857
1110	17	-0.3976494369817729
1110	18	-3.0530850549540602
1110	19	-0.7204773791536425
1110	20	-1.7954343186711483
1110	21	-0.2727415098669397
1110	22	-1.8300050682234925
1110	23	-0.5109413577712942
1110	24	0.025141885480763904
1110	25	0.04467307313043278
1111	1	-0.17305560029924294
1111	2	-1.345630118322216
1111	3	-0.39366350275642503
1111	4	-0.04519644531582401
1111	5	-0.17504809335849103
1111	6	-0.047904347191831675
1111	7	-0.05574093394704249
1111	8	-0.0047297098855025865
1111	9	-1.8336126639109054
1111	10	-0.42058866630166847
1111	11	-0.09401754635981519
1111	12	-0.1182972116890042
1111	13	-0.1462091409770565
1111	14	0.7672605157055656
1111	15	0.14883476827945685
1111	16	0.10300032456564168
1111	17	-0.1352931476788939
1111	18	0.21412094757653222
1111	19	-0.18056601664333272
1111	20	0.1698235006817157
1111	21	-0.4002812319841027
1111	22	-0.13250894213202002
1111	23	-0.03322653686244337
1111	24	-0.470870444643424
1111	25	-1.9283051267153297
1112	1	0.11483665373377505
1112	2	0.18597093897671968
1112	3	-0.013551429898061684
1112	4	0.21726269546238086
1112	5	-0.09169754716809564
1112	6	-0.08391796086745984
1112	7	0.035134145259078764
1112	8	-0.09882690643232905
1112	9	-0.4885891782354719
1112	10	0.3117687811328551
1112	11	-0.07682433030183233
1112	12	0.00283062391606137
1112	13	-0.028754086606660678
1112	14	0.2761874810890106
1112	15	-0.04373974116059287
1112	16	-0.06625414137756991
1112	17	-3.776066885784381
1112	18	-1.1934979399544687
1112	19	-1.3105658444068453
1112	20	-0.287712240655857
1112	21	-0.43007231689396974
1112	22	-1.7920862427096864
1112	23	-1.4315805258400829
1112	24	-0.5158145849832678
1112	25	-0.016676431287585558
1113	1	-0.49613317018114955
1113	2	-0.3505115141068593
1113	3	-0.3123539048784352
1113	4	-0.7259513921463849
1113	5	-0.44959754192471024
1113	6	-0.4747803128514576
1113	7	-0.5519421280003058
1113	8	-0.6358748428071763
1113	9	-0.21518879474046315
1113	10	0.038708339900345834
1113	11	-0.34634711408525476
1113	12	-0.31761551078988504
1113	13	-0.7367489515968941
1113	14	-1.743261857604118
1113	15	-2.016861848672164
1113	16	-0.8547209917384961
1113	17	-0.4270307287225279
1113	18	-0.7278342409461231
1113	19	-0.4962172201945805
1113	20	0.020067846053251515
1113	21	-1.9044085576744507
1113	22	-2.2087244972278413
1113	23	-0.9258909460614007
1113	24	-2.559430933421332
1113	25	-0.9289799829190457
1114	1	-0.10045546287553364
1114	2	0.10660382533406221
1114	3	-0.1667157704030906
1114	4	0.13337677322829222
1114	5	-0.164135407160245
1114	6	-0.03402716419132901
1114	7	-0.08469144586590716
1114	8	-0.2610724359117498
1114	9	0.18992061781081304
1114	10	0.12107403621952528
1114	11	-0.1799431820999331
1114	12	0.01011112949489501
1114	13	-0.17410714895509471
1114	14	0.6506186288345077
1114	15	0.18091592910561616
1114	16	-0.08973300814586338
1114	17	-0.6860639951976495
1114	18	-0.418381936144975
1114	19	-0.6464682095153336
1114	20	-1.6771414562723237
1114	21	-0.8689321477794323
1114	22	-1.4825742650310971
1114	23	-3.40163587296912
1114	24	-2.177398443946977
1114	25	-1.7997430699612025
1115	1	0.20583563835227636
1115	2	0.3797463349322676
1115	3	0.31797897302583067
1115	4	0.5574783431526456
1115	5	0.4107137555370173
1115	6	0.31067570810647993
1115	7	0.3736389780341369
1115	8	0.43583643011104556
1115	9	0.607542251128649
1115	10	0.79349173142203
1115	11	0.39368606545336604
1115	12	0.35228761951948684
1115	13	0.3627544643622187
1115	14	1.08281643520953
1115	15	0.6151942580674898
1115	16	0.6745145890131283
1115	17	0.7462987277979849
1115	18	0.8694051693636453
1115	19	0.3658275526538475
1115	20	0.7918789565455526
1115	21	-0.32507806974813747
1115	22	0.8851843017148084
1115	23	0.9033347256723658
1115	24	0.8101697669123666
1115	25	1.1496690490711714
1116	1	0.39746240650059406
1116	2	0.5328302041201916
1116	3	0.3883559184822438
1116	4	0.6669163952888724
1116	5	0.5308413675948458
1116	6	0.3954024924776718
1116	7	0.31686614060335216
1116	8	0.2991034187150916
1116	9	0.4533264541712754
1116	10	0.3670253831061032
1116	11	0.29378301165397147
1116	12	0.31404452973588093
1116	13	-0.1870328785001476
1116	14	0.8837220485260815
1116	15	0.5919015735654511
1116	16	0.7169932833050581
1116	17	0.7369109085115753
1116	18	0.9244765600907243
1116	19	0.8824094294142597
1116	20	0.6952127753406501
1116	21	0.2055479116677219
1116	22	0.407002292422149
1116	23	-0.5280119946344684
1116	24	-0.11727522728756412
1116	25	-0.6416201552743814
1201	1	0.5102835057581707
1201	2	0.727007865738102
1201	3	0.5970522744175852
1201	4	0.4564602394696493
1201	5	0.42945924380282463
1201	6	0.36135336171550847
1201	7	0.37958860157203844
1201	8	0.403589568750256
1201	9	0.8295239384667639
1201	10	1.0855630559717042
1201	11	0.7491890307164937
1201	12	0.5722810064016102
1201	13	0.6747343019916201
1201	14	1.4181657600658468
1201	15	1.0720536218222563
1201	16	0.8100911443098724
1201	17	0.7136632366348437
1201	18	0.8623423103687387
1201	19	0.9666087135615911
1201	20	0.9695411593576608
1201	21	0.8242179769496403
1201	22	0.9816616160468854
1201	23	0.5281723305232902
1201	24	0.9206884983504864
1201	25	1.0921802114357604
1202	1	0.3775572127438416
1202	2	0.694825249790032
1202	3	0.5027743069529613
1202	4	0.24531046998847472
1202	5	0.346984431650057
1202	6	0.16437634870759155
1202	7	0.237312652061604
1202	8	0.2816098997023815
1202	9	0.7830515094339248
1202	10	0.052736151527433606
1202	11	0.37136225668855616
1202	12	0.3830720355391648
1202	13	0.3614004724400459
1202	14	1.0609358602446761
1202	15	0.7348334046186196
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2210	15	-0.4338103642678064
2210	16	-0.12045984112748442
2210	17	0.40116020237604033
2210	18	0.3338261213618381
2210	19	0.3426891344559877
2210	20	0.674590975144433
2210	21	0.858043444585114
2210	22	0.8881489063234156
2210	23	1.2119063658267848
2210	24	1.0080086229933443
2210	25	0.8693190717052204
2211	1	0.23912683859384032
2211	2	-0.4801041158909669
2211	3	0.03512621788763326
2211	4	0.4096009766293136
2211	5	0.35243143857450576
2211	6	0.23844562634980804
2211	7	0.40937619361034905
2211	8	0.2764564245294494
2211	9	0.7125751434073848
2211	10	0.8291206701205454
2211	11	0.5303766004783981
2211	12	0.32481731813867754
2211	13	0.3512333979010549
2211	14	-0.6260890583932408
2211	15	0.24428228208563496
2211	16	0.363592451271721
2211	17	0.41247310745534804
2211	18	0.3366326051994264
2211	19	0.6837954396636192
2211	20	0.7466277363973148
2211	21	0.7942763458896849
2211	22	0.1382061416185465
2211	23	0.7408178677758528
2211	24	0.11424682344866
2211	25	0.7217406551629395
2212	1	-0.0010982544274185102
2212	2	-1.1032113221885957
2212	3	-0.4548385918065456
2212	4	0.1675381498402615
2212	5	0.10002962411940786
2212	6	0.14068400957122407
2212	7	0.11785539683904034
2212	8	0.05318832309209398
2212	9	-2.098160435961944
2212	10	-0.01803428628800674
2212	11	-0.08580066234475936
2212	12	0.013313969693783179
2212	13	0.0017826090690533647
2212	14	0.6823496207235991
2212	15	0.21148885201055379
2212	16	0.158774566843924
2212	17	0.33029712282354495
2212	18	0.001065195555121079
2212	19	-0.7241626363006688
2212	20	0.2744263961650901
2212	21	0.2930040275954795
2212	22	-2.0132826256208816
2212	23	-0.5243583854149563
2212	24	0.3019380649547472
2212	25	-0.6393602819104345
2213	1	0.01430586885330598
2213	2	0.17561305116639403
2213	3	0.12736797176729198
2213	4	0.219040632032943
2213	5	0.1143909281169897
2213	6	0.02099212057524369
2213	7	0.02784889786577338
2213	8	0.17773900535456733
2213	9	0.5366255454543705
2213	10	0.5169085266388578
2213	11	-0.08240978088421969
2213	12	-0.03425864433336301
2213	13	-0.0027701746578330244
2213	14	-1.531746146382254
2213	15	-0.3489918125710787
2213	16	-0.2592569058286074
2213	17	-2.3556637977216397
2213	18	-0.8968539655195329
2213	19	-0.5531867136345596
2213	20	0.23183567970841779
2213	21	-0.4087791038718791
2213	22	0.16066310730374767
2213	23	0.661812551851142
2213	24	0.4736108435966031
2213	25	-1.0493095484085515
2214	1	0.024330861910790702
2214	2	-0.013551574821338619
2214	3	0.0802383865510663
2214	4	0.2858554057069256
2214	5	0.19381331078847097
2214	6	0.18552217535800963
2214	7	0.09896045778599927
2214	8	0.06606043122111771
2214	9	0.4033860412762351
2214	10	0.5123327774782709
2214	11	0.004268162111415563
2214	12	-0.1944654050816439
2214	13	-0.17602634087428726
2214	14	0.7777903460062277
2214	15	0.37404724223692654
2214	16	0.317648499235645
2214	17	0.252529633395973
2214	18	0.24684727908423554
2214	19	-0.9726686757158021
2214	20	0.2610636719328028
2214	21	0.4715495927023099
2214	22	0.5449237924669603
2214	23	1.0222957509625061
2214	24	0.8025005459520074
2214	25	0.7278016075834728
2215	1	-0.035622286923377544
2215	2	0.4013897869330556
2215	3	0.35135013599142795
2215	4	0.4980258132031068
2215	5	0.46222690852218307
2215	6	0.3551753974899867
2215	7	0.1705639958909515
2215	8	0.23848956016583145
2215	9	0.34086103087587105
2215	10	0.7802753872764043
2215	11	0.5883902722958467
2215	12	0.3703041030306409
2215	13	0.22035037023330517
2215	14	0.991754073496864
2215	15	0.5978690295148041
2215	16	0.6065255570220529
2215	17	0.7922327772775901
2215	18	0.6429446661164704
2215	19	0.02881362385431885
2215	20	-1.4422594921552374
2215	21	-1.5470071338572495
2215	22	0.16788383525702955
2215	23	0.6366670736907665
2215	24	0.5996642179740377
2215	25	0.039190006719749704
2216	1	-0.01864091451422567
2216	2	-0.2084401394940727
2216	3	-0.16570919105118875
2216	4	0.13698988274067858
2216	5	0.2071226633064569
2216	6	0.08236526033059022
2216	7	0.11071776522675947
2216	8	-0.0923009915287949
2216	9	0.09345748062825265
2216	10	-1.8285385813787016
2216	11	-0.6142683373603361
2216	12	-0.3383829982798119
2216	13	-0.08728306975691424
2216	14	-0.5287775465328934
2216	15	-0.44995537385202056
2216	16	-0.03399913370694918
2216	17	0.3352408071143458
2216	18	0.29155472990679243
2216	19	-1.2598900873976433
2216	20	-0.759467595361059
2216	21	-0.6911635933921758
2216	22	-0.6343814346799945
2216	23	-4.066165787499035
2216	24	-1.8371155933044179
2216	25	-1.0860586328954724
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	11.0
5	28.0
6	25.0
7	110.0
8	63.0
9	182.0
10	491.0
11	1269.0
12	2843.0
13	5025.0
14	8188.0
15	12087.0
16	16711.0
17	19016.0
18	18952.0
19	18117.0
20	16652.0
21	15628.0
22	14611.0
23	14054.0
24	13842.0
25	14280.0
26	15119.0
27	16795.0
28	19650.0
29	23584.0
30	29543.0
31	37096.0
32	50675.0
33	68751.0
34	95374.0
35	135306.0
36	198980.0
37	299939.0
38	475486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.12167688606234	19.60500693060285	12.56946443366686	19.70385174966795
2	12.387453398319384	25.22467105937636	38.49537028661164	23.892505255692612
3	20.433922218006952	32.271185704644104	30.178474818651225	17.116417258697712
4	12.177604542715532	21.993313078342418	32.31714562662898	33.51193675231307
5	9.595575105929136	46.16905711004083	31.77313067649869	12.462237107531344
6	27.254021743587764	34.44383067307669	23.326293724174363	14.97585385916118
7	21.53835576949751	41.02567504489846	19.060949736639916	18.375019448964107
8	21.065342588370402	38.3665438612999	23.899577057351795	16.668536492977896
9	26.927152980971698	23.78624994646839	17.480377272680663	31.806219799879244
10	14.912770571647057	30.188836273547242	27.4248623169352	27.473530837870502
11	27.143608404968877	31.48027756033488	19.428258517563425	21.947855517132826
12	25.087501778981725	30.06804755851342	23.07218685700364	21.772263805501215
13	27.44521083703379	24.02020590677806	30.111770555849393	18.422812700338756
14	27.812239875502154	27.945966486916866	22.914359475468373	21.327434162112603
15	30.766683555298208	25.50774464389114	23.627678054429644	20.097893746381008
16	23.08872955478723	27.707247409916224	28.67621826962683	20.527804765669714
17	19.571924644456587	34.834992395212545	25.420977151865376	20.1721058084655
18	19.791106275496542	26.086927677272588	35.6944950388042	18.427471008426675
19	22.141433950612818	34.74729894997359	22.882760317540722	20.228506781872873
20	20.726380015896005	30.672661640569316	29.03837595385092	19.562582389683755
21	22.591802867452675	33.19363506551145	23.670052389865504	20.54450967717037
22	20.399828730981	31.075255848173633	21.68736167312946	26.83755374771591
23	24.503666305031512	26.555699552951406	23.592321595166833	25.348312546850245
24	19.45626943736352	30.909998787850277	21.313873807374335	28.319857967411867
25	23.74211881501261	31.910484948641777	23.16618722665582	21.1812090096898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14841.0
1	14841.0
2	11532.5
3	8224.0
4	8224.0
5	8224.0
6	10855.0
7	13486.0
8	13486.0
9	13486.0
10	12960.0
11	12434.0
12	12434.0
13	12434.0
14	13888.5
15	15343.0
16	15343.0
17	15343.0
18	19030.5
19	22718.0
20	22718.0
21	22718.0
22	30221.0
23	37724.0
24	37724.0
25	37724.0
26	47391.0
27	57058.0
28	57058.0
29	57058.0
30	65842.0
31	74626.0
32	74626.0
33	74626.0
34	82830.5
35	91035.0
36	91035.0
37	91035.0
38	120023.5
39	149012.0
40	149012.0
41	149012.0
42	297957.5
43	446903.0
44	446903.0
45	446903.0
46	354594.5
47	262286.0
48	262286.0
49	262286.0
50	217395.0
51	172504.0
52	172504.0
53	172504.0
54	137087.0
55	101670.0
56	101670.0
57	101670.0
58	85353.5
59	69037.0
60	69037.0
61	69037.0
62	62217.0
63	55397.0
64	55397.0
65	55397.0
66	41118.5
67	26840.0
68	26840.0
69	26840.0
70	22078.5
71	17317.0
72	17317.0
73	17317.0
74	12112.5
75	6908.0
76	6908.0
77	6908.0
78	4668.0
79	2428.0
80	2428.0
81	2428.0
82	1432.5
83	437.0
84	437.0
85	437.0
86	314.0
87	191.0
88	191.0
89	191.0
90	122.5
91	54.0
92	54.0
93	54.0
94	31.0
95	8.0
96	8.0
97	8.0
98	6.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08091722264596907
2	0.017063766027428647
3	0.029665628570653337
4	0.03750410766452516
5	0.04660880261202242
6	0.015013702264416017
7	0.018028501915905177
8	0.03479078797818491
9	0.03533345191545296
10	0.019837381706798673
11	0.019475605748619975
12	0.014048966375939487
13	0.021465373518602823
14	0.0376849956436145
15	0.034067236061827516
16	0.020138861671947592
17	0.01923442177650084
18	0.025143429093419597
19	0.01453133432017775
20	0.012481270557165124
21	0.008984102961437698
22	0.016581398083190382
23	0.016641694076220168
24	0.016521102090160597
25	0.1019605242133634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1658485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.10905903896865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9329487853717	15.269401501280225
2	8.2558838685814	3.320361122643445
3	3.7038778771552536	2.234444967145346
4	2.175579183618233	1.7499540098932103
5	1.515765558719964	1.5240309554767528
6	1.125069156287125	1.3574449252020262
7	0.8852066131008562	1.2460470431171418
8	0.7003964235926053	1.1267450426164956
9	0.5615021037239437	1.0162151058860895
>10	4.418667044372074	17.48984562138122
>50	0.42347752056971977	5.816407334614572
>100	0.2346979366744763	9.480088583195467
>500	0.03121302482595036	4.493414366534506
>1k	0.027911647200128682	11.15830669779725
>5k	0.003901628103243795	5.766124964649822
>10k+	0.003901628103243795	16.95116775856643
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	70800	4.2689563065086515	No Hit
GCTTATGTACTCTGCGTTGATACCA	42657	2.572046174671462	No Hit
CTTATGTACTCTGCGTTGATACCAC	28599	1.7244051046587698	No Hit
GAGTACATAAGCAGTGGTATCAACG	20565	1.2399870966574915	No Hit
TATGTACTCTGCGTTGATACCACTG	19223	1.1590698740115226	No Hit
GCGTTGATACCACTGCTTATGTACT	15105	0.9107709747148753	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13507	0.8144179778532817	No Hit
CCCATGTACTCTGCGTTGATACCAC	13212	0.7966306599094957	No Hit
CATAAGCAGTGGTATCAACGCAGAG	12846	0.7745623264605951	No Hit
ACTCTGCGTTGATACCACTGCTTAT	11647	0.702267430817885	No Hit
CTGCTTATGTACTCTGCGTTGATAC	11337	0.6835756729786522	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	11136	0.6714561783796658	No Hit
GTATCAACGCAGAGTACATAAGCAG	10236	0.6171897846528609	No Hit
CATGTACTCTGCGTTGATACCACTG	9825	0.59240813151762	No Hit
GTACATGATAAGCAGTGGTATCAAC	9214	0.5555672797764225	No Hit
ATCATGTACTCTGCGTTGATACCAC	9169	0.5528539600900823	No Hit
TATCAACGCAGAGTACATAAGCAGT	8644	0.5211985637494461	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8494	0.5121541647949786	No Hit
GGTATCAACGCAGAGTACATAAGCA	8384	0.5055216055617024	No Hit
GTGGTATCAACGCAGAGTACATGGG	6720	0.40518907316014313	No Hit
GTGGTATCAACGCAGAGTACATAAG	6551	0.3949990503381098	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	6499	0.39186365870056106	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5907	0.3561684308269294	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5501	0.3316882576568374	No Hit
ACATAAGCAGTGGTATCAACGCAGA	5432	0.32752783413778236	No Hit
GTACTGGTTCACTATCGGTCAGTCA	5201	0.3135994597479025	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4930	0.2972592456368312	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4557	0.27476884023672205	No Hit
GATACCACTGCTTATGTACTCTGCG	4548	0.274226176299454	No Hit
GTACTCTGCGTTGATACCACTGCTT	4522	0.27265848048067964	No Hit
CCACTGCTTATGTACTCTGCGTTGA	4433	0.26729213710102895	No Hit
ATACAGGGTGACAGCCCCGTACACA	3892	0.23467200487191625	No Hit
ATACCACTGCTTATGTACTCTGCGT	3871	0.2334057890182908	No Hit
GTTGATACCACTGCTTATGTACTCT	3760	0.22671293379198484	No Hit
GAGTACATGATAAGCAGTGGTATCA	3651	0.2201406705517385	No Hit
GGATACCACGTGTCCCGCCCTACTC	3508	0.2115183435484795	No Hit
CAGTGGTATCAACGCAGAGTACATA	3145	0.18963089807866818	No Hit
GCTTATCATGTACTCTGCGTTGATA	3041	0.18336011480357073	No Hit
TGATACCACTGCTTATGTACTCTGC	2914	0.17570252368878825	No Hit
TACCACTGCTTATGTACTCTGCGTT	2830	0.17063766027428645	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2704	0.16304036515253378	No Hit
GCGTTGATACCACTGCTTATCATGT	2576	0.15532247804472152	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2549	0.1536944862329174	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2541	0.15321211828867912	No Hit
GGTATCAACGCAGAGTACATGGGGG	2420	0.14591630313207535	No Hit
GTTAATGATAGTGTGTCGAAACACA	2407	0.14513245522268817	No Hit
CCCCATGTACTCTGCGTTGATACCA	2373	0.14308239145967555	No Hit
GATAAGCAGTGGTATCAACGCAGAG	2331	0.14054995975242465	No Hit
GTATCAACGCAGAGTACATGGGGGT	2330	0.14048966375939487	No Hit
GTACATGGGATAAGCAGTGGTATCA	2287	0.13789693605911418	No Hit
GGTATCAACGCAGAGTACATGGGAA	2284	0.13771604808002486	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2281	0.13753516010093547	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2279	0.1374145681148759	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2278	0.13735427212184614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2220	0.1338571045261187	No Hit
GCTTATCCCATGTACTCTGCGTTGA	2200	0.13265118466552306	No Hit
GTATCAACGCAGAGTACATGGGAAG	2173	0.1310231928537189	No Hit
TATCAACGCAGAGTACATGATAAGC	2101	0.1266818813555745	No Hit
ATCAACGCAGAGTACATAAGCAGTG	2096	0.1263804013904256	No Hit
CCCATATTCAGACAGGATACCACGT	2069	0.12475240957862145	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	2020	0.12179790592016207	No Hit
GCGTTGATACCACTGCTTCCCATGT	2017	0.12161701794107273	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1998	0.12047139407350685	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1953	0.1177580743871666	No Hit
ATCATTAACTGAATCCATAGGTTAA	1933	0.11655215452657094	No Hit
ATGTACTCTGCGTTGATACCACTGC	1906	0.11492416271476678	No Hit
GTATCAACGCAGAGTACATGATAAG	1898	0.11444179477052853	No Hit
CAGTGGTATCAACGCAGAGTACATG	1872	0.11287409895175415	No Hit
TATCAACGCAGAGTACATGGGAAGC	1830	0.11034166724450327	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1829	0.11028137125147347	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1798	0.1084121954675502	No Hit
GGTATCAACGCAGAGTACATGATAA	1773	0.10690479564180562	No Hit
TATTCAGACAGGATACCACGTGTCC	1767	0.10654301968362694	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1702	0.10262378013669103	No Hit
GTACACAAGCAGTGGTATCAACGCA	1682	0.10141786027609535	No Hit
ATGATAAGCAGTGGTATCAACGCAG	1659	0.10003105243641035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.0295993029783204E-5	0.0
2	0.0	0.0	0.0	6.0295993029783204E-5	0.0
3	0.0	0.0	0.0	6.0295993029783204E-5	0.0
4	0.0	0.0	0.0	6.0295993029783204E-5	0.0
5	0.0	0.0	0.0	6.0295993029783204E-5	0.0
6	6.0295993029783204E-5	0.0	0.0	1.2059198605956641E-4	0.0
7	6.0295993029783204E-5	0.0	0.0	1.2059198605956641E-4	0.0
8	6.0295993029783204E-5	0.0	0.0	1.8088797908934963E-4	0.0
9	6.0295993029783204E-5	0.0	0.0	1.8088797908934963E-4	0.0
10	6.0295993029783204E-5	0.0	0.0	2.4118397211913282E-4	0.0
11	6.0295993029783204E-5	0.0	0.0	3.0147996514891603E-4	0.0
12	6.0295993029783204E-5	0.0	0.0	3.6177595817869925E-4	0.0
13	6.0295993029783204E-5	0.0	0.0	4.8236794423826563E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	45	3.5316058E-5	16.893072	2
CGTTGTA	35	0.0021666936	16.28975	2
CGTGACT	35	0.002172377	16.283361	13
CGCTTGT	35	0.002172377	16.283361	14
ATGCCCG	45	6.748432E-4	14.781884	5
TCAGTAC	40	0.005269577	14.25396	3
TCGTATC	40	0.005284363	14.247942	13
CCGGATA	40	0.005286478	14.247082	18
CTCTAAC	55	1.948164E-4	13.828281	1
TTCGTTG	165	0.0	13.816185	13
CCGGTTA	50	0.0015012542	13.2980795	13
CGGGTCG	50	0.0015015954	13.297677	8
TCTGATC	50	0.0015015954	13.297677	9
GGGTCGT	50	0.0015015954	13.297677	9
GTCCAGG	65	5.4156648E-5	13.163459	1
GAGCGCC	65	5.4618577E-5	13.151548	9
TACACTG	80	1.9934014E-6	13.06613	5
GTACAAA	375	0.0	12.929442	1
GTCCATG	60	4.0671384E-4	12.675924	1
TCGTACA	60	4.0837404E-4	12.669805	2
>>END_MODULE