FastQCFastQC Report
Wed 25 May 2016
SRR1295343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658485
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA749144.5170140218331785No Hit
GCTTATGTACTCTGCGTTGATACCA475262.8656273647334767No Hit
CTTATGTACTCTGCGTTGATACCAC331201.9970032891464196No Hit
GTATCAACGCAGAGTACTTTTTTTT259941.5673340428161846No Hit
GAGTACATAAGCAGTGGTATCAACG225651.360579082717058No Hit
TATGTACTCTGCGTTGATACCACTG186441.124158494047278No Hit
GGTATCAACGCAGAGTACTTTTTTT171481.0339556884747225No Hit
GCGTTGATACCACTGCTTATGTACT170901.0304585208789951No Hit
CCCATGTACTCTGCGTTGATACCAC159160.9596710250620295No Hit
CATAAGCAGTGGTATCAACGCAGAG157540.9499030741912046No Hit
TATCAACGCAGAGTACTTTTTTTTT152240.9179461978854195No Hit
ACTCTGCGTTGATACCACTGCTTAT137970.8319038158319189No Hit
CTGCTTATGTACTCTGCGTTGATAC132270.7975350998049425No Hit
ACGCAGAGTACATAAGCAGTGGTAT131420.7924099403974108No Hit
GTACTTTTTTTTTTTTTTTTTTTTT119270.7191503088662242No Hit
CATGTACTCTGCGTTGATACCACTG112320.6772445937105249No Hit
GTATCAACGCAGAGTACATAAGCAG110320.6651853951045683No Hit
ATCATGTACTCTGCGTTGATACCAC102920.6205663602625288No Hit
TATCAACGCAGAGTACATAAGCAGT98110.591563987615203No Hit
GTACATGATAAGCAGTGGTATCAAC94380.5690735822150939No Hit
GGTATCAACGCAGAGTACATAAGCA91120.5494170884873846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT87880.5298811867457348No Hit
AAGCAGTGGTATCAACGCAGAGTAC74080.44667271636463396No Hit
GTGGTATCAACGCAGAGTACATGGG70870.4273177026020735No Hit
GTGGTATCAACGCAGAGTACATAAG67590.4075406168883047No Hit
ACATAAGCAGTGGTATCAACGCAGA66470.40078746566896895No Hit
GTACTGGTTCACTATCGGTCAGTCA56200.3388634808273816No Hit
GTACATGGGAAGCAGTGGTATCAAC55920.3371751930225477No Hit
GATACCACTGCTTATGTACTCTGCG52650.3174584033018086No Hit
GCAGTGGTATCAACGCAGAGTACAT51800.312333243894277No Hit
CCACTGCTTATGTACTCTGCGTTGA50370.30371091689101803No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47460.2861647829193511No Hit
GTACTCTGCGTTGATACCACTGCTT46480.2802557756024323No Hit
ATACCACTGCTTATGTACTCTGCGT44380.2675936170661779No Hit
ATACAGGGTGACAGCCCCGTACACA43870.26451852142165894No Hit
GGATACCACGTGTCCCGCCCTACTC38970.23497348483706515No Hit
GAGTACATGATAAGCAGTGGTATCA38290.2308733573110399No Hit
CAGTGGTATCAACGCAGAGTACATA36970.22291428623110854No Hit
GTTGATACCACTGCTTATGTACTCT36920.22261280626595958No Hit
GCTTATCATGTACTCTGCGTTGATA35020.21115656759030077No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34130.2057902242106501No Hit
TGATACCACTGCTTATGTACTCTGC32870.19819292908889738No Hit
TACCACTGCTTATGTACTCTGCGTT32450.1956604973816465No Hit
GCAGAGTACATAAGCAGTGGTATCA29640.17871732334027743No Hit
GCGTTGATACCACTGCTTATCATGT29240.1763054836190861No Hit
GAGTACATGGGAAGCAGTGGTATCA28150.16973322037883973No Hit
GATAAGCAGTGGTATCAACGCAGAG27870.1680449325740058No Hit
CCCCATGTACTCTGCGTTGATACCA27590.16635664476917186No Hit
ACGCAGAGTACATGATAAGCAGTGG26610.1604476374522531No Hit
ACCCTGTATCGCGCGCCTTTCCAGA26120.15749313379379373No Hit
GGTATCAACGCAGAGTACATGGGGG25500.15375478222594716No Hit
GTTAATGATAGTGTGTCGAAACACA24990.15067968658142825No Hit
ATCAACGCAGAGTACATAAGCAGTG24810.14959435870689214No Hit
GGTATCAACGCAGAGTACATGGGAA24800.14953406271386235No Hit
GTGGTATCAACGCAGAGTACTTTTT24580.14820755086720713No Hit
CCCATATTCAGACAGGATACCACGT24330.14670015104146253No Hit
ATCAACGCAGAGTACTTTTTTTTTT24180.14579571114601578No Hit
TCTAAGTACCCCGAGGAAAAGAAAT23640.1425397275224075No Hit
GTACATGGGATAAGCAGTGGTATCA23560.14205735957816923No Hit
GTATCAACGCAGAGTACATGGGAAG23500.14169558361999052No Hit
GTATCAACGCAGAGTACATGGGGGT23140.13952492787091833No Hit
GCTTATCCCATGTACTCTGCGTTGA22950.13837930400335247No Hit
GCGTTGATACCACTGCTTCCCATGT22460.13542480034489307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22330.1346409524355059No Hit
TATCAACGCAGAGTACATGATAAGC22160.13361592055399957No Hit
CAGTGGTATCAACGCAGAGTACATG22070.13307325661673153No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21160.12758632125102126No Hit
ATCATTAACTGAATCCATAGGTTAA21140.1274657292649617No Hit
ATGTACTCTGCGTTGATACCACTGC20940.12625980940436604No Hit
GTATCAACGCAGAGTACATGATAAG20820.12553625748800865No Hit
GTATTTAGCCTTGGAGGATGGTCCC20400.12300382578075773No Hit
CTATCGGTCAGTCAGGAGTATTTAG20330.12258175382954925No Hit
ACGCAGAGTACATGGGAAGCAGTGG20220.12191849790622164No Hit
TATCAACGCAGAGTACATGGGAAGC20020.12071257804562598No Hit
GCCTTGGAGGATGGTCCCCCCATAT19660.11854192229655378No Hit
TATTCAGACAGGATACCACGTGTCC19540.1178183703801964No Hit
GTTCACTATCGGTCAGTCAGGAGTA19340.11661245051960072No Hit
ACTCTGCGTTGATACCACTGCTTCC19320.11649185853354115No Hit
ATGATAAGCAGTGGTATCAACGCAG19250.11606978658233266No Hit
GGTTAATGAGGCGAACCGGGGGAAC19190.11570801062415396No Hit
TACATAAGCAGTGGTATCAACGCAG18850.11365794686114135No Hit
GTCAGGAGTATTTAGCCTTGGAGGA18240.10997989128632456No Hit
CTGCTTATCATGTACTCTGCGTTGA18180.10961811532814586No Hit
GTACACAAGCAGTGGTATCAACGCA18010.10859308344663954No Hit
GGTATCAACGCAGAGTACATGATAA17680.1066033156766567No Hit
GCCCAGAGCCTGAATCAGTGTGTGT17310.10437236393455473No Hit
AAAAAGTACTCTGCGTTGATACCAC16970.10232230017154209No Hit
CATCTAAGTACCCCGAGGAAAAGAA16960.10226200417851232No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG16830.10147815626912514No Hit
GGATAAGCAGTGGTATCAACGCAGA16820.10141786027609535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT250.005967600719.0354751
TGAAGGC250.00601147719.0113495
CATACCG402.74871E-416.6349285
GGAAGTA350.002175188616.280218
CTTATAC1700.016.2361371
CCTCTAA601.4721354E-615.82798213
GTATAGG400.0052143714.2766051
GTCGTAC602.523681E-514.2766041
TTATGAC400.005258431714.258513
CACTACA400.005258431714.258512
ATACCGT801.2939199E-714.2451836
ACTATAT551.9503746E-413.8264343
ATTAACA551.9503746E-413.8264342
CCCGAAC551.9659064E-413.8135116
CCGTTGG707.1246377E-613.59676551
GCCTCTA707.288654E-613.56684112
TCCAAGG1351.8189894E-1213.3783552
GTAAGAC1009.988071E-913.3079433
TGACGAC500.001503445613.29550514
ACGGACT500.001503445613.2955058
Produced by FastQC (version 0.11.5)