FastQCFastQC Report
Wed 25 May 2016
SRR1295342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345378
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG56650.1303684052342512No Hit
GTATCAACGCAGAGTACTTTTTTTT53580.12330342722773485No Hit
GGTATCAACGCAGAGTACTTTTTTT43760.10070470278995292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1855.820766E-1110.2815981
CTAGGCG856.5738335E-410.0628424
CGTCAGA2002.5465852E-119.978872
AGACCGT1356.474984E-79.8492086
ACGATTC900.00111710019.497458
GGTATCA41300.09.2341441
GTCTTAG4950.08.8380191
GTTCTAG4700.08.7010761
GTCTTAC4100.08.5826261
GTCCTAG4850.08.2358781
GTGGTAT22500.08.2001461
GTCTTAA4300.08.18343451
CTAGACT4050.07.97849464
TGGTATC21700.07.92703772
GCTAGAC2053.40493E-77.8811963
TAGGACC2201.2658893E-77.77583274
GTATTAG7350.07.76365571
TAGACTG4900.07.75820065
TAGACAG5700.07.66972975
CTAGAAC4850.07.6422273
Produced by FastQC (version 0.11.5)