FastQCFastQC Report
Wed 25 May 2016
SRR1295342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345378
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144150.3317317848988051No Hit
GGTATCAACGCAGAGTACTTTTTTT118710.2731868205711908No Hit
TATCAACGCAGAGTACTTTTTTTTT84810.19517289404972363No Hit
GTGGTATCAACGCAGAGTACATGGG63390.14587913870784086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54070.12443106215385634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3650.014.586083
CAGCGTC4350.012.4574462
GCAGCGT4550.011.7165771
ACCGGGC700.001497222310.8528898
GGTATCA50550.010.640241
CTAGAAC4650.09.4047733
TATTCCG2501.8189894E-129.1264025
GGACCGC1252.7682092E-59.1167416
CTAATAC6900.09.0936513
CTGTGCG1804.2504325E-88.9689139
CTACACT5200.08.9584144
TTAGACG850.00739514368.94765954
ATACCGT1606.7839574E-78.9030686
GTCCTAG4750.08.8182661
TAGACTG5500.08.8152755
CCTATAC4950.08.6427263
TACCGCA1107.1767584E-48.632987
TGGACCG1000.0028881688.5560035
GTGGTAT23350.08.5208621
GCGTCAG6300.08.4505674
Produced by FastQC (version 0.11.5)