FastQCFastQC Report
Wed 25 May 2016
SRR1295341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421440
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA773375.440750225123818No Hit
GCTTATGTACTCTGCGTTGATACCA447113.1454721972084645No Hit
CTTATGTACTCTGCGTTGATACCAC298322.09871679423683No Hit
GAGTACATAAGCAGTGGTATCAACG205441.445294912201711No Hit
TATGTACTCTGCGTTGATACCACTG187831.3214064610535794No Hit
GCGTTGATACCACTGCTTATGTACT151481.0656798739306619No Hit
CCCATGTACTCTGCGTTGATACCAC146721.0321927059882936No Hit
CATAAGCAGTGGTATCAACGCAGAG137780.9692987393066186No Hit
CTGCTTATGTACTCTGCGTTGATAC114860.8080538045925258No Hit
ACGCAGAGTACATAAGCAGTGGTAT114040.8022850067537146No Hit
ACTCTGCGTTGATACCACTGCTTAT113710.7999634173795588No Hit
CATGTACTCTGCGTTGATACCACTG102830.7234213192255741No Hit
GTATCAACGCAGAGTACATAAGCAG100170.7047079018460153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA95870.6744568887888338No Hit
TATCAACGCAGAGTACATAAGCAGT87760.6174020711391265No Hit
GGTATCAACGCAGAGTACATAAGCA84120.5917942368302567No Hit
GTACATGATAAGCAGTGGTATCAAC76630.539101193156236No Hit
ATCATGTACTCTGCGTTGATACCAC73860.5196139126519586No Hit
AAGCAGTGGTATCAACGCAGAGTAC67390.4740966906798739No Hit
GTACATGGGAAGCAGTGGTATCAAC66640.46882035119315624No Hit
GTGGTATCAACGCAGAGTACATAAG62740.44138338586222425No Hit
GTATCAACGCAGAGTACTTTTTTTT56090.39459984241332735No Hit
ACATAAGCAGTGGTATCAACGCAGA55270.388831044574516No Hit
GTACTCTGCGTTGATACCACTGCTT46350.3260777802791535No Hit
GCAGTGGTATCAACGCAGAGTACAT46270.3255149707339036No Hit
GTGGTATCAACGCAGAGTACATGGG45770.32199741107609187No Hit
CCACTGCTTATGTACTCTGCGTTGA43700.30743471409275097No Hit
GATACCACTGCTTATGTACTCTGCG42960.3022287257991895No Hit
GTTGATACCACTGCTTATGTACTCT38820.2731033318325079No Hit
GGTATCAACGCAGAGTACTTTTTTT37970.2671234804142278No Hit
ATACCACTGCTTATGTACTCTGCGT37480.26367627194957227No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36560.25720396217919855No Hit
TATCAACGCAGAGTACTTTTTTTTT34480.2425709140027015No Hit
GTACTGGTTCACTATCGGTCAGTCA30530.2147821927059883No Hit
CAGTGGTATCAACGCAGAGTACATA30510.21464149031967583No Hit
GAGTACATGATAAGCAGTGGTATCA29480.2073953174245835No Hit
GTACATGGGATAAGCAGTGGTATCA29340.2064104007203962No Hit
GAGTACATGGGAAGCAGTGGTATCA29180.20528478162989647No Hit
TACCACTGCTTATGTACTCTGCGTT28320.19923457901846017No Hit
TGATACCACTGCTTATGTACTCTGC27860.19599842413327329No Hit
GCTTATCCCATGTACTCTGCGTTGA26150.18396837010355696No Hit
GCAGAGTACATAAGCAGTGGTATCA25830.18171713192255742No Hit
GAACAAAAAAAAAAAAAAAAAAAAA25600.18009905447996397No Hit
GCTTATCATGTACTCTGCGTTGATA24540.17264182800540295No Hit
GATAAGCAGTGGTATCAACGCAGAG23480.16518460153084197No Hit
GTATCAACGCAGAGTACATGGGAAG22560.15871229176046825No Hit
CCCCATGTACTCTGCGTTGATACCA22280.15674245835209363No Hit
GCGTTGATACCACTGCTTCCCATGT22210.15625No Hit
ATCAACGCAGAGTACATAAGCAGTG20970.14752645204862674No Hit
GCGTTGATACCACTGCTTATCATGT20830.14654153534443945No Hit
GGATACCACGTGTCCCGCCCTACTC20590.14485310670868978No Hit
GGTATCAACGCAGAGTACATGGGAA20560.14464205312922107No Hit
GTACACAAGCAGTGGTATCAACGCA20170.14189835659612787No Hit
TATCAACGCAGAGTACATGGGAAGC19440.13676271949572266No Hit
ATACAGGGTGACAGCCCCGTACACA19230.13528534443944168No Hit
GGATAAGCAGTGGTATCAACGCAGA18670.13134567762269247No Hit
CAGTGGTATCAACGCAGAGTACATG18490.13007935614588023No Hit
ACGCAGAGTACATGGGAAGCAGTGG18110.12740601080594327No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17870.1257175821701936No Hit
GAGTAAGCAGTGGTATCAACGCAGA17770.12501407023863123No Hit
ACTCTGCGTTGATACCACTGCTTCC17170.12079299864925709No Hit
ACGCAGAGTACATGATAAGCAGTGG17010.11966737955875731No Hit
GAGTACATGTAAGCAGTGGTATCAA16970.11938597478613237No Hit
AAAAAGTACTCTGCGTTGATACCAC16890.11882316524088248No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA16710.11755684376407025No Hit
ATGTACTCTGCGTTGATACCACTGC16620.11692368302566411No Hit
TATCAACGCAGAGTACATGATAAGC16550.11643122467357046No Hit
GTACATAAGCAGTTGTATCAACGCA16290.11460209365150834No Hit
GGGATAAGCAGTGGTATCAACGCAG16220.11410963529941467No Hit
CATGTAAGCAGTGGTATCAACGCAG15860.11157699234579019No Hit
GCTTACATGTACTCTGCGTTGATAC15230.10714486717694732No Hit
TACATAAGCAGTGGTATCAACGCAG15110.10630065285907248No Hit
GTATCAACGCAGAGTACATGATAAG14710.10348660513282304No Hit
GGTATCAACGCAGAGTACATGATAA14250.1002504502476362No Hit
GAGTACATGGGATAAGCAGTGGTAT14230.10010974786132372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT350.002173354416.2820349
GTCTTAC508.636025E-515.215841
CTAGACT400.005267011514.2548064
TTAGCAC400.005267011514.2548063
TCCCACA400.005268241714.2543042
AAATTGT400.005285493614.2472826
CGCACCT551.9637257E-413.8150612
GTATCTA707.278464E-613.56836215
TACAGTA852.6885573E-713.4158142
GAACAAA12100.013.2038291
GTATATA655.3988202E-513.1675551
CGAACTC655.4634154E-513.15087415
CTCCAAA1155.2405085E-912.4042181
CGCAAAA2000.012.3537312
ACATAAT1405.2750693E-1112.2184063
CCACAGT701.08734486E-412.2184064
ATGTGAG701.09271765E-412.2119557
CATAAGG1251.4024408E-912.1641014
GCTGCGT1251.4151738E-912.15725310
CCCACAG550.00306048312.0949873
Produced by FastQC (version 0.11.5)