FastQCFastQC Report
Wed 25 May 2016
SRR1295341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421440
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA798015.614095565060784No Hit
GCTTATGTACTCTGCGTTGATACCA516473.633428072940117No Hit
CTTATGTACTCTGCGTTGATACCAC358292.520612899594777No Hit
GAGTACATAAGCAGTGGTATCAACG226241.5916253939666818No Hit
TATGTACTCTGCGTTGATACCACTG182651.2849645429986492No Hit
CCCATGTACTCTGCGTTGATACCAC181991.2803213642503377No Hit
GCGTTGATACCACTGCTTATGTACT177611.2495075416479062No Hit
CATAAGCAGTGGTATCAACGCAGAG163921.1531967582170193No Hit
GTATCAACGCAGAGTACTTTTTTTT157831.1103528815848716No Hit
CTGCTTATGTACTCTGCGTTGATAC135240.9514295362449348No Hit
ACTCTGCGTTGATACCACTGCTTAT133830.9415100180099054No Hit
ACGCAGAGTACATAAGCAGTGGTAT130500.9180830706888788No Hit
CATGTACTCTGCGTTGATACCACTG120960.8509680324178297No Hit
GGTATCAACGCAGAGTACTTTTTTT109900.7731596127870328No Hit
GTACTTTTTTTTTTTTTTTTTTTTT106430.748747748761819No Hit
GTATCAACGCAGAGTACATAAGCAG106310.7479035344439442No Hit
TATCAACGCAGAGTACTTTTTTTTT105070.7391799864925709No Hit
TATCAACGCAGAGTACATAAGCAGT97920.6888788833858622No Hit
GGTATCAACGCAGAGTACATAAGCA88160.620216118865376No Hit
ATCATGTACTCTGCGTTGATACCAC84850.5969298739306619No Hit
AAGCAGTGGTATCAACGCAGAGTAC76060.5350911751463304No Hit
GTACATGATAAGCAGTGGTATCAAC74500.5241163890139576No Hit
GTGGTATCAACGCAGAGTACATAAG66550.4681871904547501No Hit
ACATAAGCAGTGGTATCAACGCAGA66450.4674836785231878No Hit
GTACATGGGAAGCAGTGGTATCAAC64690.45510186852769025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63110.4439863800090049No Hit
GCAGTGGTATCAACGCAGAGTACAT53090.37349448446645656No Hit
CCACTGCTTATGTACTCTGCGTTGA52130.36674076992345794No Hit
GATACCACTGCTTATGTACTCTGCG50190.35309263845114813No Hit
GTACTCTGCGTTGATACCACTGCTT49830.35055999549752365No Hit
GTGGTATCAACGCAGAGTACATGGG47510.33423851868527693No Hit
ATACCACTGCTTATGTACTCTGCGT42620.29983678523187757No Hit
GTTGATACCACTGCTTATGTACTCT39650.27894248086447543No Hit
CAGTGGTATCAACGCAGAGTACATA36800.2588923908149482No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36470.2565708014407924No Hit
CTTATACACATCTCCGAGCCCACGA35150.24728444394416926No Hit
TACCACTGCTTATGTACTCTGCGTT33680.23694281855020263No Hit
GTACTGGTTCACTATCGGTCAGTCA32700.23004840162089152No Hit
GAGTACATGATAAGCAGTGGTATCA32450.2282896217919856No Hit
TGATACCACTGCTTATGTACTCTGC32130.22603838361098605No Hit
GAGTACATGGGAAGCAGTGGTATCA30940.21766659162539398No Hit
GTACATGGGATAAGCAGTGGTATCA29870.2101390139576767No Hit
GCTTATCCCATGTACTCTGCGTTGA29470.2073249662314273No Hit
GCAGAGTACATAAGCAGTGGTATCA29020.20415916253939667No Hit
GCTTATCATGTACTCTGCGTTGATA28810.2026817874831157No Hit
CCCCATGTACTCTGCGTTGATACCA28690.2018375731652409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27030.19015927510130573No Hit
GCGTTGATACCACTGCTTCCCATGT26560.18685276902296263No Hit
GCGTTGATACCACTGCTTATCATGT24970.1756669293111211No Hit
GATAAGCAGTGGTATCAACGCAGAG24720.17390814948221522No Hit
ATCAACGCAGAGTACATAAGCAGTG24320.1710941017559658No Hit
GCAGAGTACTTTTTTTTTTTTTTTT24240.1705312922107159No Hit
GTATCAACGCAGAGTACATGGGAAG23780.16729513732552903No Hit
ACGCAGAGTACATGGGAAGCAGTGG22540.15857158937415577No Hit
ATACAGGGTGACAGCCCCGTACACA22500.15829018460153085No Hit
GGATACCACGTGTCCCGCCCTACTC22010.15484297613687528No Hit
CAGTGGTATCAACGCAGAGTACATG22010.15484297613687528No Hit
GGTATCAACGCAGAGTACATGGGAA21980.15463192255740657No Hit
GGATAAGCAGTGGTATCAACGCAGA21380.15041085096803242No Hit
ACGCAGAGTACATGATAAGCAGTGG21290.1497776902296263No Hit
ACTCTGCGTTGATACCACTGCTTCC20770.14611942818550203No Hit
TATCAACGCAGAGTACATGGGAAGC20420.14365713642503378No Hit
GGGATAAGCAGTGGTATCAACGCAG19980.1405616839261594No Hit
AAAAAGTACTCTGCGTTGATACCAC19380.13634061233678524No Hit
GTACACAAGCAGTGGTATCAACGCA19190.13500393966681673No Hit
GAGTAAGCAGTGGTATCAACGCAGA19020.13380796938316075No Hit
ATGTACTCTGCGTTGATACCACTGC18420.12958689779378657No Hit
CATGTAAGCAGTGGTATCAACGCAG18360.12916479063484917No Hit
TACATAAGCAGTGGTATCAACGCAG18220.12817987393066185No Hit
TATCAACGCAGAGTACATGATAAGC18170.1278281179648807No Hit
GCTTACATGTACTCTGCGTTGATAC17990.12656179648806845No Hit
GAGTACATGTAAGCAGTGGTATCAA17500.12311458802341288No Hit
GTGGTATCAACGCAGAGTACTTTTT17220.12114475461503828No Hit
GAGTACATGGGATAAGCAGTGGTAT16810.1182603556956326No Hit
GTATCAACGCAGAGTACATGATAAG16070.11305436740207113No Hit
ATACACATCTCCGAGCCCACGAGAC15910.11192874831157137No Hit
TACATGTACTCTGCGTTGATACCAC15730.11066242683475912No Hit
ATCAACGCAGAGTACTTTTTTTTTT15350.10798908149482216No Hit
ATGATAAGCAGTGGTATCAACGCAG14890.1047529266096353No Hit
CTGCTTATCATGTACTCTGCGTTGA14830.10433081945069789No Hit
AAAGTACTCTGCGTTGATACCACTG14690.10334590274651058No Hit
CCCATATTCAGACAGGATACCACGT14490.10193887888338586No Hit
TCCATGTACTCTGCGTTGATACCAC14430.10151677172444845No Hit
GGTATCAACGCAGAGTACATGATAA14400.10130571814497974No Hit
CCATGTACTCTGCGTTGATACCACT14240.10018009905447996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGT451.635919E-619.0388221
GACTTGA250.00604416518.9932813
CTTATAC3800.017.5357571
TATTAAG555.8676414E-717.2842522
CTCGCAT402.768367E-416.61912211
TCCCTAT350.002140686616.318991
TCGGGCA350.002160429716.296584
CGTGGTC350.002175209816.27995514
TCGCATT350.002175209816.27995512
ACCGGTC350.002175209816.2799558
TTATACA4400.015.7718792
TATACAC4350.015.7346293
GTGTTAT508.562704E-515.2310581
GAGACCG1200.015.03952119
TTTACTC456.727715E-414.7876383
TTAGGAT602.5518542E-514.2595073
TAGACTG400.00525550114.2595075
CGCAAAA602.5518542E-514.2595072
ACAAGGC400.00525550114.2595073
GGACCGC400.00528997514.2454626
Produced by FastQC (version 0.11.5)