FastQCFastQC Report
Wed 25 May 2016
SRR1295340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340705
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA915292.7398109081765676No Hit
GCTTATGTACTCTGCGTTGATACCA546151.6348345633631225No Hit
CTTATGTACTCTGCGTTGATACCAC345241.0334345594717282No Hit
GAGTACATAAGCAGTGGTATCAACG236940.709251490329137No Hit
TATGTACTCTGCGTTGATACCACTG224620.672373047006545No Hit
CCCATGTACTCTGCGTTGATACCAC201200.6022680841319422No Hit
GCGTTGATACCACTGCTTATGTACT183030.5478783669913986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA168700.5049832295877666No Hit
GTATCAACGCAGAGTACTTTTTTTT167720.5020497170507423No Hit
CATAAGCAGTGGTATCAACGCAGAG152400.4561911333086878No Hit
CATGTACTCTGCGTTGATACCACTG147640.4419426438431409No Hit
ACTCTGCGTTGATACCACTGCTTAT145800.4364348243858706No Hit
GTACATGATAAGCAGTGGTATCAAC145360.4351177371243495No Hit
ATCATGTACTCTGCGTTGATACCAC137570.41179930583514557No Hit
CTGCTTATGTACTCTGCGTTGATAC130010.38916935197810043No Hit
ACGCAGAGTACATAAGCAGTGGTAT127790.3825240480676983No Hit
GTATCAACGCAGAGTACATAAGCAG125290.37504059771814635No Hit
GTGGTATCAACGCAGAGTACATGGG112790.3376233459703865No Hit
GGTATCAACGCAGAGTACTTTTTTT107950.3231353860936539No Hit
TATCAACGCAGAGTACATAAGCAGT102330.30631258970786107No Hit
GGTATCAACGCAGAGTACATAAGCA96700.28945985952067005No Hit
TATCAACGCAGAGTACTTTTTTTTT94830.2838622386592052No Hit
GTACATGGGAAGCAGTGGTATCAAC87080.2606635425755941No Hit
AAGCAGTGGTATCAACGCAGAGTAC85760.2567122807910306No Hit
GTACTGGTTCACTATCGGTCAGTCA80140.23988948440523783No Hit
GTGGTATCAACGCAGAGTACATAAG72360.2166009869174321No Hit
GCAGTGGTATCAACGCAGAGTACAT68750.20579488461267906No Hit
GTACTCTGCGTTGATACCACTGCTT63530.19016944028281457No Hit
ATACAGGGTGACAGCCCCGTACACA58870.17622028883124968No Hit
GTACATGGGATAAGCAGTGGTATCA58530.17520253958371063No Hit
ACATAAGCAGTGGTATCAACGCAGA57920.17337657769841994No Hit
GGATACCACGTGTCCCGCCCTACTC55120.16499511330692176No Hit
GAGTACATGATAAGCAGTGGTATCA54620.16349842323701136No Hit
GCTTATCCCATGTACTCTGCGTTGA51780.1549972236399203No Hit
CCACTGCTTATGTACTCTGCGTTGA50440.15098609425256046No Hit
GATACCACTGCTTATGTACTCTGCG49240.14739403808477552No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47870.14329310729322103No Hit
GCTTATCATGTACTCTGCGTTGATA46120.13805469204853466No Hit
ATACCACTGCTTATGTACTCTGCGT45660.1366777371842171No Hit
GTTGATACCACTGCTTATGTACTCT45590.13646820057442965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40860.12230951251307734No Hit
GCGTTGATACCACTGCTTATCATGT40390.12090262384736156No Hit
GATAAGCAGTGGTATCAACGCAGAG39810.11916646336626549No Hit
CAGTGGTATCAACGCAGAGTACATA37750.11300010027823469No Hit
GAGTACATGGGAAGCAGTGGTATCA36170.10827055965731784No Hit
GTATCAACGCAGAGTACATGGGAAG35930.10755214842376086No Hit
ACCCTGTATCGCGCGCCTTTCCAGA35610.10659426677901819No Hit
GTATTTAGCCTTGGAGGATGGTCCC35410.10599559075105405No Hit
GGTATCAACGCAGAGTACATGGGAA34740.10399002605737412No Hit
CCCATATTCAGACAGGATACCACGT34350.10282260780284402No Hit
GGATAAGCAGTGGTATCAACGCAGA34120.10213413037068522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG456.7740673E-414.7752279
TATGACG400.005271114.25394254
TAACGCG400.005289986714.2462617
TACGCTG1101.8735591E-1013.8160047
ACGCTGT551.9648347E-413.81517712
CTTCGTT2550.013.78131612
GCCACGA655.468828E-513.15059315
TTCGTTG2750.012.77922913
GCTGCGT1052.0012521E-812.6644810
AGTTGTA3600.012.4000811
CTGTCGT701.0933851E-412.2121769
ACAGTGC1900.011.9981088
GCGTTAC951.0434997E-611.9975713
ACGCTGC1353.7289283E-1011.9610778
TAGGACT2250.011.8254954
TCGTTGA3150.011.75952214
GAGCGGT658.044337E-411.68941618
TTAGGAC1406.7848305E-1011.5389063
CTCTTCG3150.011.4583410
CCCGTAC15100.011.44732716
Produced by FastQC (version 0.11.5)