##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295340_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3340705
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.015356638793307	31.0	30.0	33.0	26.0	34.0
2	29.79693148601867	31.0	30.0	34.0	25.0	34.0
3	30.366692659184213	31.0	30.0	34.0	26.0	34.0
4	33.41818328765934	35.0	35.0	37.0	27.0	37.0
5	34.142522910583246	37.0	35.0	37.0	30.0	37.0
6	34.21029513231488	37.0	35.0	37.0	30.0	37.0
7	34.166198152785114	37.0	35.0	37.0	30.0	37.0
8	34.118679141079504	37.0	35.0	37.0	29.0	37.0
9	35.044630998546715	39.0	35.0	39.0	27.0	39.0
10	35.00963838471221	39.0	35.0	39.0	27.0	39.0
11	35.44280084592923	39.0	35.0	39.0	28.0	39.0
12	35.46792189073863	39.0	35.0	39.0	28.0	39.0
13	35.27001007272417	39.0	35.0	39.0	27.0	39.0
14	35.53382654260104	39.0	34.0	41.0	25.0	41.0
15	36.105288853700046	39.0	36.0	41.0	27.0	41.0
16	36.175289048269754	39.0	36.0	41.0	27.0	41.0
17	36.133723270986216	39.0	36.0	41.0	27.0	41.0
18	36.09332580997125	39.0	36.0	41.0	27.0	41.0
19	36.15503224618756	39.0	36.0	41.0	27.0	41.0
20	36.195971209669814	39.0	36.0	41.0	27.0	41.0
21	36.22059325800991	39.0	36.0	41.0	27.0	41.0
22	36.01695749849208	39.0	35.0	41.0	27.0	41.0
23	35.72206076262346	39.0	34.0	41.0	25.0	41.0
24	36.058235911282196	39.0	35.0	41.0	27.0	41.0
25	35.0745429482699	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5369609712160788
1101	2	0.6719084424603885
1101	3	0.49617254664524424
1101	4	0.43724416014880774
1101	5	0.4418667122400137
1101	6	0.41128466879223424
1101	7	0.2720981193611749
1101	8	0.3953298833233987
1101	9	0.4380193515330433
1101	10	0.7199717719851222
1101	11	0.5957017948243006
1101	12	0.5811820487835178
1101	13	0.6194262713539871
1101	14	1.2461647328993095
1101	15	0.8942062231026568
1101	16	0.6332039251439099
1101	17	0.907340441030783
1101	18	0.912393479765754
1101	19	0.7685489292647318
1101	20	0.8912469506099399
1101	21	0.9494990095553959
1101	22	0.8458608467789546
1101	23	-1.2468437959234464
1101	24	0.23624170368352537
1101	25	0.7347953002323209
1102	1	0.42105965522730315
1102	2	0.4356467190076465
1102	3	0.49193028084221524
1102	4	0.5921397827573216
1102	5	0.4770729743225104
1102	6	0.40721098284556234
1102	7	0.40362303869903116
1102	8	0.5147517413707448
1102	9	0.7597704165874788
1102	10	1.120319104459007
1102	11	0.7939237495128779
1102	12	0.6683608354349104
1102	13	0.5981284551690464
1102	14	1.2938395463768728
1102	15	0.9971201581772533
1102	16	0.8616858238000162
1102	17	1.150452602828551
1102	18	1.1322834157259791
1102	19	1.0289164501815335
1102	20	1.17875105991925
1102	21	1.1430007282658323
1102	22	0.9838642628819159
1102	23	1.1272084449450972
1102	24	1.0155053154550586
1102	25	0.9733066250960789
1103	1	-0.170013691616564
1103	2	-1.254483529241579
1103	3	-0.161322065048914
1103	4	0.08443615180421915
1103	5	0.01672777374357537
1103	6	0.1883274753527857
1103	7	0.1594742945243226
1103	8	0.2765562482878039
1103	9	0.9164003636518387
1103	10	1.022999355863135
1103	11	0.6848978640744292
1103	12	0.5289603481307239
1103	13	0.2951726303869222
1103	14	-0.7960355275476516
1103	15	0.11657650479106962
1103	16	0.1606015190785257
1103	17	0.6192826142449661
1103	18	0.8039961210245181
1103	19	0.7442587329708132
1103	20	0.6956667936051986
1103	21	0.7652307386632771
1103	22	0.45281635508798956
1103	23	1.1761230624638301
1103	24	1.1348158705640472
1103	25	0.8998428932803364
1104	1	0.1463632827279575
1104	2	0.1056189157069376
1104	3	-0.08529124009565336
1104	4	-0.037773095781183486
1104	5	0.0876273038231048
1104	6	0.17057832131401796
1104	7	0.16659031114180323
1104	8	0.25369449080613293
1104	9	-1.804841105275898
1104	10	-0.16640906532241218
1104	11	-0.12898663779645148
1104	12	0.29942167352218974
1104	13	0.3813711463071954
1104	14	-1.4236542541740462
1104	15	-0.163376637382612
1104	16	0.08694465497368498
1104	17	0.4447246839828978
1104	18	0.6802813681664972
1104	19	0.6502515238450144
1104	20	0.7366147474154943
1104	21	0.7582997473264541
1104	22	0.9369404204543059
1104	23	1.279220272320373
1104	24	0.9618880716838447
1104	25	0.9188972162453979
1105	1	-0.06775317146601267
1105	2	-0.005563055643154513
1105	3	-1.3985562432269951
1105	4	-0.3676130577394119
1105	5	-0.324112739917247
1105	6	-0.006063148200595947
1105	7	0.01517547077057202
1105	8	-0.025980070584104453
1105	9	0.38834056506220094
1105	10	0.5312400406311681
1105	11	0.020868221134783482
1105	12	0.10995704519158522
1105	13	0.006136319747952257
1105	14	0.7310351837856786
1105	15	0.3572984936260113
1105	16	-0.004825259525951253
1105	17	0.44816716656848854
1105	18	0.29105274859932706
1105	19	0.3678525879007992
1105	20	-0.2655508106196933
1105	21	0.221722829761255
1105	22	0.3725140847376025
1105	23	0.4858144512669611
1105	24	0.3865715830199221
1105	25	0.5730166757584456
1106	1	-1.4838430987397935
1106	2	-0.9732032535710324
1106	3	-0.9933786172990828
1106	4	-1.3218925942264335
1106	5	-0.7722447121317302
1106	6	-0.6466224418921627
1106	7	-0.6418487745768076
1106	8	-0.6597601683529248
1106	9	-0.33991548002423855
1106	10	-1.8690549556479468
1106	11	-1.2277190092659822
1106	12	-0.7736550331586116
1106	13	-0.97609833620551
1106	14	-0.2711206175909666
1106	15	-0.856981868751852
1106	16	-1.2733787353667694
1106	17	-0.6131081744856033
1106	18	-0.6484824250169723
1106	19	-0.6374870072553236
1106	20	-0.25386322095641134
1106	21	-0.3376361719784171
1106	22	-0.370120740528634
1106	23	-0.6646003788939367
1106	24	-0.7092731523810514
1106	25	-1.5992968609987628
1107	1	0.09037702476150145
1107	2	0.28440009052771487
1107	3	0.06247144826109263
1107	4	0.22128357911707752
1107	5	0.10121305800291935
1107	6	0.08264513239073068
1107	7	-0.03452298700119627
1107	8	0.009631622980890597
1107	9	0.4605342234657712
1107	10	0.11400469033887362
1107	11	0.1257849932128039
1107	12	0.012511004433299888
1107	13	0.14358495010515782
1107	14	-0.8842400929777057
1107	15	-0.3153883220675695
1107	16	-0.104661099008311
1107	17	0.2561099983385091
1107	18	0.4206883759002409
1107	19	0.3586810481161251
1107	20	0.4271064339467827
1107	21	0.538957373528639
1107	22	-0.021534328119450663
1107	23	0.7708744926874687
1107	24	0.5324400719388152
1107	25	0.26571651355974524
1108	1	-0.10005862654371711
1108	2	0.1799248556176991
1108	3	-0.09208993238724261
1108	4	-0.245041400251381
1108	5	-0.29031135960845234
1108	6	-0.20774274060583764
1108	7	-0.18375925797659676
1108	8	-0.14948172743680743
1108	9	0.3105363952070732
1108	10	0.24778974586305935
1108	11	0.028574956872333246
1108	12	-0.1443408393750616
1108	13	-0.13141541185172656
1108	14	0.16335289374571715
1108	15	-0.06355609632229431
1108	16	-0.08596095856903929
1108	17	0.14129148351999277
1108	18	0.15060194059640963
1108	19	0.2159049836009288
1108	20	0.3171757848856984
1108	21	0.1947595423742854
1108	22	0.21752226745888237
1108	23	0.06223820144248293
1108	24	0.08176900387644537
1108	25	-0.7158960767484999
1109	1	-0.6422510706572915
1109	2	-0.6007690039437037
1109	3	-0.5346580372404865
1109	4	-0.42844085665387155
1109	5	-0.6969412424769601
1109	6	-0.899797231446982
1109	7	-0.5860278129246268
1109	8	-0.9696459469943548
1109	9	-0.6755224846839667
1109	10	-0.831065226824073
1109	11	-0.9131698503932029
1109	12	-0.8770304618523497
1109	13	-0.88551514876238
1109	14	-0.4412835663002497
1109	15	-1.020852367066496
1109	16	-1.1880588382200017
1109	17	-1.0743017088173872
1109	18	-1.6117799309866712
1109	19	-1.7174784512991863
1109	20	-1.5957322261011768
1109	21	-1.3288136151725851
1109	22	-1.4552293153028089
1109	23	-0.9843609695247366
1109	24	-1.7922796527809055
1109	25	-1.0222616581393567
1110	1	0.011953857144696656
1110	2	0.15242144565823068
1110	3	-0.013984076466510231
1110	4	0.20753948813766243
1110	5	0.1651635160290752
1110	6	0.10973674620752405
1110	7	0.084764454392662
1110	8	-0.025812143346030325
1110	9	0.3451664681638391
1110	10	0.20632860339783576
1110	11	0.13559156988718257
1110	12	0.19023041429752396
1110	13	0.2557640491191435
1110	14	-2.4949646764943907
1110	15	-0.7443795539112372
1110	16	-0.18220549646493822
1110	17	-0.18909727199218906
1110	18	-2.7288924510256223
1110	19	-0.42108122654466484
1110	20	-1.7475999713685084
1110	21	-0.30767969757360447
1110	22	-1.6339697259635173
1110	23	-0.44461687277647144
1110	24	0.16465711435882469
1110	25	0.10476994504323756
1111	1	-0.3762762212368358
1111	2	-1.4728175029619202
1111	3	-0.5343855989737882
1111	4	-0.0247670956489614
1111	5	-0.1621107718196484
1111	6	0.02019775608944485
1111	7	-0.09551816310972328
1111	8	-0.10077912496134189
1111	9	-1.7955223144547503
1111	10	-0.42082144070892014
1111	11	-0.2640407981243271
1111	12	-0.19988944146840737
1111	13	-0.11127731609212077
1111	14	0.6886444532065923
1111	15	0.14418585690207664
1111	16	0.2624712648494949
1111	17	-0.20864363593653934
1111	18	0.053618921425240273
1111	19	-0.10990628394727509
1111	20	0.1765222574180214
1111	21	-0.6673632579809095
1111	22	-0.2825196903642606
1111	23	-0.05946745755372973
1111	24	-0.6257913691641619
1111	25	-2.038678084285735
1112	1	0.15163567019280677
1112	2	0.4176253784292605
1112	3	0.04097448000153392
1112	4	0.30468866324158483
1112	5	0.19256151769670282
1112	6	0.16201204844828965
1112	7	0.19671987086036324
1112	8	0.11120774470089856
1112	9	-0.24870881167655057
1112	10	0.46655070547481614
1112	11	0.13760506762280755
1112	12	0.13300250334206254
1112	13	0.143358557792439
1112	14	0.5110638836212402
1112	15	0.11268935565039584
1112	16	0.17823897453924786
1112	17	-3.6690420678207545
1112	18	-1.0066308276779594
1112	19	-1.152222197319496
1112	20	-0.0632207448696036
1112	21	-0.49033755216916575
1112	22	-1.5173306181603436
1112	23	-1.4364551081673156
1112	24	-0.3190362694655846
1112	25	0.042427811853777087
1113	1	-0.2818216238214042
1113	2	-0.12089024955248462
1113	3	-0.1300416090618377
1113	4	-0.5006898110684546
1113	5	-0.21554719975133452
1113	6	-0.26012520601003075
1113	7	-0.34389713520123166
1113	8	-0.278020051562045
1113	9	0.19975917463201398
1113	10	0.25926863253410204
1113	11	-0.1200518583945609
1113	12	-0.19901186229303391
1113	13	-0.4520812249472712
1113	14	-1.5961271385538751
1113	15	-1.7020878133701274
1113	16	-0.5017582646845256
1113	17	-0.30070086924621364
1113	18	-0.5302591755476485
1113	19	-0.17730458066469623
1113	20	0.1683814697848618
1113	21	-1.5067785116668873
1113	22	-1.9566654744914231
1113	23	-0.6984339173814504
1113	24	-2.4364042258581193
1113	25	-0.7501647032887888
1114	1	5.015599631832401E-4
1114	2	0.09936277929257997
1114	3	-0.07922171321443017
1114	4	0.09707405468017072
1114	5	-0.0981751127024495
1114	6	-0.08021513548821702
1114	7	-0.05798057537676726
1114	8	-0.1462807446579646
1114	9	0.25098226819349634
1114	10	0.032864651232287656
1114	11	-0.002168521519990918
1114	12	-0.02720708160604346
1114	13	-0.10462404408401937
1114	14	0.6975770828625372
1114	15	0.1912118505387781
1114	16	0.03408480670202607
1114	17	-0.6494327616463522
1114	18	-0.28951611832244595
1114	19	-0.7198694739620848
1114	20	-1.7398848336779125
1114	21	-0.9645266688217404
1114	22	-1.6383509693310927
1114	23	-3.087843109346686
1114	24	-2.146686907951519
1114	25	-1.7636843276000747
1115	1	0.31585155023602596
1115	2	0.4217627278903535
1115	3	0.18950156100549265
1115	4	0.4994482715674806
1115	5	0.21612671577371856
1115	6	0.17062857751702865
1115	7	0.33767624234089055
1115	8	0.33433549054190337
1115	9	0.574848480637165
1115	10	0.7125204602832085
1115	11	0.3886506989356491
1115	12	0.2301933221156176
1115	13	0.2301412509471774
1115	14	0.92721987562512
1115	15	0.38391237723313054
1115	16	0.42663617514610763
1115	17	0.40464717544710993
1115	18	0.6062028614147223
1115	19	0.05792466164545118
1115	20	0.5422056204745402
1115	21	-0.4172721112722755
1115	22	0.6382344745064898
1115	23	0.6839899486600629
1115	24	0.7217793041351896
1115	25	0.7733374640378372
1116	1	0.2722842410932138
1116	2	0.379019304471111
1116	3	0.15962434815950388
1116	4	0.4795302954419327
1116	5	0.17424182437910218
1116	6	0.06742023057659452
1116	7	0.054455827215207364
1116	8	0.022462087131316366
1116	9	0.1338919111845982
1116	10	0.21329842722500558
1116	11	-0.0024074057293290707
1116	12	-0.01323705954074228
1116	13	-0.4402472683627465
1116	14	0.509004044089032
1116	15	0.16228015517852157
1116	16	0.2194728451889958
1116	17	0.34308616894720245
1116	18	0.4147747541471247
1116	19	0.5465193977284954
1116	20	0.34245676471422115
1116	21	-0.1071285363376333
1116	22	-0.03351685456871678
1116	23	-1.062256895459761
1116	24	-0.4314937766835243
1116	25	-1.089579721356344
1201	1	0.4252509946225267
1201	2	0.64450353324548
1201	3	0.5627656751730932
1201	4	0.4395568071832301
1201	5	0.4339250397226664
1201	6	0.2620713575891713
1201	7	0.289978137413641
1201	8	0.4108733493304726
1201	9	0.6407789571617499
1201	10	0.9327613435217543
1201	11	0.530363632864784
1201	12	0.6521827218444471
1201	13	0.6265075495074868
1201	14	1.270454177376621
1201	15	0.8523704075548366
1201	16	0.5813481893737134
1201	17	0.7656439070527412
1201	18	0.6477444518346545
1201	19	0.8451204930542744
1201	20	0.926688129214412
1201	21	0.8568533257213957
1201	22	0.8829198458342873
1201	23	0.5613982053854656
1201	24	0.9331117471143742
1201	25	0.9998283896043176
1202	1	0.49138016938097806
1202	2	0.76831777146419
1202	3	0.6108335635707114
1202	4	0.45814423412419814
1202	5	0.35271588656343056
1202	6	0.27985496511578845
1202	7	0.25654679868945607
1202	8	0.30433134969617015
1202	9	0.8859499829061548
1202	10	0.11221978265636068
1202	11	0.39920476851203546
1202	12	0.4206108760458136
1202	13	0.4479485493488653
1202	14	1.1444638019607964
1202	15	0.8508321068877933
1202	16	0.7136069582746529
1202	17	-1.366492588100762
1202	18	0.22204826034501934
1202	19	0.6590081999014785
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2210	21	0.5326682190380438
2210	22	0.748671964388997
2210	23	1.095826758646929
2210	24	0.878937219874814
2210	25	0.6995680337421959
2211	1	0.04421055926485451
2211	2	-0.6742801080505529
2211	3	-0.12079475768440062
2211	4	0.2197755131022774
2211	5	0.10657915168354037
2211	6	0.0658616246716548
2211	7	0.13983112630450023
2211	8	0.20612900983139326
2211	9	0.6081628598457698
2211	10	0.6331916191726847
2211	11	0.3748251412700583
2211	12	0.19201140937964567
2211	13	0.17535478202060517
2211	14	-1.027644886218681
2211	15	-0.15567366468272326
2211	16	0.06050020492256181
2211	17	0.2350427146938614
2211	18	0.17426432306919537
2211	19	0.35629748337362344
2211	20	0.5052494776522565
2211	21	0.5667736780832016
2211	22	-0.17939623323290732
2211	23	0.35558704987909096
2211	24	-0.22191540149201217
2211	25	0.23447200270111068
2212	1	-0.10841706665907935
2212	2	-1.1991258809968741
2212	3	-0.4654733405022071
2212	4	0.04639536941622424
2212	5	0.02386340023537059
2212	6	-0.03284362682104813
2212	7	-0.007491203381604805
2212	8	-0.013374416050012883
2212	9	-2.278397678684925
2212	10	-0.19392902678151813
2212	11	-0.10041288074884136
2212	12	-0.1827158719539952
2212	13	-0.09017593587468298
2212	14	0.48053169196330714
2212	15	0.15178863842660917
2212	16	0.23317623202535742
2212	17	0.19646292808877774
2212	18	0.013720210237543995
2212	19	-0.5966377825955576
2212	20	0.16270131739177884
2212	21	0.20504338559980084
2212	22	-1.7954587977096068
2212	23	-0.34530999628238135
2212	24	0.24133425149436505
2212	25	-0.5463731408714736
2213	1	0.05845140115097536
2213	2	0.34949254730507207
2213	3	-0.04991769432498572
2213	4	0.17642734126302884
2213	5	0.07789807976453034
2213	6	0.10065032194953005
2213	7	0.03796513756569908
2213	8	0.006797695420488026
2213	9	0.4776951806704872
2213	10	0.5805451520653264
2213	11	-0.07238794981790875
2213	12	-0.00755164626392002
2213	13	-0.08221769364059384
2213	14	-1.9646113628456874
2213	15	-0.6060508324237333
2213	16	-0.31955167888794733
2213	17	-2.3833188441537914
2213	18	-1.047840469433858
2213	19	-0.718697153550913
2213	20	-0.009595594877779945
2213	21	-0.39240273244853796
2213	22	0.14491891229440057
2213	23	0.6408920025299238
2213	24	0.3818025256776352
2213	25	-1.141362184547404
2214	1	0.1619684721601935
2214	2	0.13223624060939798
2214	3	0.12485937994913954
2214	4	0.3972134679464929
2214	5	0.18913271087913586
2214	6	0.1540734254548184
2214	7	0.2697501206484034
2214	8	0.1709354560106746
2214	9	0.5106683542096349
2214	10	0.4682540469484948
2214	11	-0.13458892233239794
2214	12	-0.21662101745400975
2214	13	-0.10107384954462617
2214	14	0.81349960381133
2214	15	0.3457781135292919
2214	16	0.26423723753467243
2214	17	0.286229755650524
2214	18	0.29155781133285785
2214	19	-0.8583303620439509
2214	20	0.15983736471082466
2214	21	0.4104386868391572
2214	22	0.5874216396957195
2214	23	0.9702099547995218
2214	24	0.815755740043862
2214	25	0.7995840556226597
2215	1	-0.019796143451262793
2215	2	0.3151102462982287
2215	3	0.1536611534536938
2215	4	0.4385917497811178
2215	5	0.2668876538078848
2215	6	0.1469867781906089
2215	7	0.16453914513674306
2215	8	0.1416098009271991
2215	9	0.28311733808171624
2215	10	0.5890676605249681
2215	11	0.3232615227994273
2215	12	0.23765923460325666
2215	13	0.07923886644473299
2215	14	0.8304849695020664
2215	15	0.3331708445022201
2215	16	0.25874922316589277
2215	17	0.46170887395186355
2215	18	0.3629654453622422
2215	19	-0.4049250222982508
2215	20	-1.5441991958812977
2215	21	-1.8284673794103625
2215	22	-0.21688608725682457
2215	23	0.4083452466236963
2215	24	0.5876020675160731
2215	25	-0.13347354473219752
2216	1	0.0031240902056595132
2216	2	-0.23059052819734305
2216	3	-0.18981295091127137
2216	4	0.14775931638119744
2216	5	0.07027325859839095
2216	6	-0.11667059721546025
2216	7	0.025232717567782004
2216	8	-0.15025342819046728
2216	9	0.1639367683620918
2216	10	-1.7464025833464092
2216	11	-0.6296304227835279
2216	12	-0.3482321098367862
2216	13	-0.1591227924366052
2216	14	-0.5008278083206079
2216	15	-0.631064899984807
2216	16	-0.10664823128589518
2216	17	0.10932989969118978
2216	18	-0.050631114506387576
2216	19	-1.4860540845858097
2216	20	-1.033035606278247
2216	21	-0.975230175513687
2216	22	-0.9218136707224431
2216	23	-4.711384818617489
2216	24	-2.191660201539051
2216	25	-1.353799209690422
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	18.0
5	47.0
6	60.0
7	278.0
8	181.0
9	396.0
10	951.0
11	2213.0
12	4683.0
13	8515.0
14	13848.0
15	20496.0
16	27783.0
17	31192.0
18	31545.0
19	29967.0
20	28734.0
21	27267.0
22	24831.0
23	24286.0
24	24199.0
25	24495.0
26	26093.0
27	29615.0
28	34382.0
29	43167.0
30	53426.0
31	68309.0
32	94612.0
33	131378.0
34	186942.0
35	271275.0
36	407892.0
37	640512.0
38	1027107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.21100952611488	19.633119651334248	12.56414803914461	20.59172278340626
2	14.102010993113662	23.70474337375838	38.87334207361416	23.319903559513797
3	19.410174919798234	30.703667572735533	29.901649907686373	19.98450759977986
4	12.396469752952777	20.377256000404852	34.38538240799549	32.84089183864688
5	10.636947672816746	43.70437453431178	32.83793690838131	12.820740884490156
6	28.007557739887286	36.267600995403804	21.55099528404695	14.173845980661959
7	23.61723512594188	38.08927144458087	20.3964499326795	17.89704349679775
8	22.443336477563673	36.95090508916139	23.044812768569674	17.560945664705265
9	26.435155281697835	21.31697254066317	18.887318768646423	33.360553408992565
10	14.87230478941623	30.168932898111507	29.63263037941786	25.326131933054402
11	28.1864907695638	29.670570179548818	20.98118463477209	21.16175441611529
12	24.107104364362733	29.917991559645333	25.72164553384525	20.253258542146686
13	27.306811230699534	24.00310070927677	28.64347562083799	20.046612439185708
14	25.596764432231943	26.649982945951393	24.623321134473045	23.12993148734362
15	28.35254939315421	27.130173820422993	24.119765915721636	20.397510870701165
16	22.21091119875158	28.947893175500646	27.729656026453426	21.111539599294353
17	19.536322122380348	32.69697612058631	26.467418055610448	21.299283701422898
18	19.757323806897446	27.085008229635733	33.65859632386648	19.499071639600345
19	21.779103083431384	32.25989563217978	25.41009128441878	20.55090999997006
20	21.88281575110547	29.69142658695112	29.01976223051844	19.405995431424973
21	23.200008861421146	31.039133353012076	25.31142207248283	20.44943571308395
22	21.10116711246998	29.727086937334246	24.40960252548025	24.76214342471553
23	23.485939244458375	26.937274123281718	25.545404981555613	24.031381650704287
24	20.44629443229238	29.89549358369046	23.978602920527102	25.679609063490055
25	22.6316043394005	30.9097632316026	25.058141050593274	21.400491378403622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18803.0
1	18803.0
2	15036.0
3	11269.0
4	11269.0
5	11269.0
6	15486.0
7	19703.0
8	19703.0
9	19703.0
10	20283.5
11	20864.0
12	20864.0
13	20864.0
14	25948.0
15	31032.0
16	31032.0
17	31032.0
18	44286.5
19	57541.0
20	57541.0
21	57541.0
22	75594.5
23	93648.0
24	93648.0
25	93648.0
26	121416.0
27	149184.0
28	149184.0
29	149184.0
30	171188.0
31	193192.0
32	193192.0
33	193192.0
34	212381.0
35	231570.0
36	231570.0
37	231570.0
38	274367.5
39	317165.0
40	317165.0
41	317165.0
42	510348.5
43	703532.0
44	703532.0
45	703532.0
46	596196.5
47	488861.0
48	488861.0
49	488861.0
50	422364.0
51	355867.0
52	355867.0
53	355867.0
54	299686.0
55	243505.0
56	243505.0
57	243505.0
58	208092.0
59	172679.0
60	172679.0
61	172679.0
62	148105.5
63	123532.0
64	123532.0
65	123532.0
66	91862.5
67	60193.0
68	60193.0
69	60193.0
70	45397.0
71	30601.0
72	30601.0
73	30601.0
74	21714.0
75	12827.0
76	12827.0
77	12827.0
78	8332.0
79	3837.0
80	3837.0
81	3837.0
82	2365.5
83	894.0
84	894.0
85	894.0
86	587.0
87	280.0
88	280.0
89	280.0
90	187.5
91	95.0
92	95.0
93	95.0
94	58.0
95	21.0
96	21.0
97	21.0
98	15.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08150974120732
2	0.01837935405849963
3	0.030023602802402488
4	0.03657910530861001
5	0.04672666398260248
6	0.016553392173208947
7	0.021312866595523998
8	0.03756692075475087
9	0.03765672215894549
10	0.023917107317168083
11	0.021612204609506077
12	0.01679286258439461
13	0.02466545235212328
14	0.03957248544843079
15	0.03663897291140643
16	0.02385723971437167
17	0.022659887658443352
18	0.02831737612270464
19	0.01781061183193368
20	0.014817231692112893
21	0.011464645935513612
22	0.019965845532604644
23	0.01987604412841002
24	0.018648758271083497
25	0.1066242005804164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3340705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.376397014933577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9969415452632	13.950413232419853
2	8.742114276137558	3.3878135392871997
3	3.8908259360580266	2.261705641591827
4	2.459218470965058	1.9060317375950746
5	1.7279757608677022	1.6740972187377259
6	1.3219856935025922	1.5369191787221115
7	1.0843928771598714	1.4708138835611038
8	0.8308262820298512	1.2878735912841257
9	0.7312123157059562	1.2751434118194802
>10	6.2561603255965	24.65184666954751
>50	0.6393109499562525	8.461110864498464
>100	0.26584118860401945	9.14478389692787
>500	0.02581491880975299	3.5286844530405017
>1k	0.021903567474941932	8.909928243927268
>5k	0.0023468108008866356	3.1419539756570165
>10k+	0.0031290810678488478	13.410880461382845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	91529	2.7398109081765676	No Hit
GCTTATGTACTCTGCGTTGATACCA	54615	1.6348345633631225	No Hit
CTTATGTACTCTGCGTTGATACCAC	34524	1.0334345594717282	No Hit
GAGTACATAAGCAGTGGTATCAACG	23694	0.709251490329137	No Hit
TATGTACTCTGCGTTGATACCACTG	22462	0.672373047006545	No Hit
CCCATGTACTCTGCGTTGATACCAC	20120	0.6022680841319422	No Hit
GCGTTGATACCACTGCTTATGTACT	18303	0.5478783669913986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	16870	0.5049832295877666	No Hit
GTATCAACGCAGAGTACTTTTTTTT	16772	0.5020497170507423	No Hit
CATAAGCAGTGGTATCAACGCAGAG	15240	0.4561911333086878	No Hit
CATGTACTCTGCGTTGATACCACTG	14764	0.4419426438431409	No Hit
ACTCTGCGTTGATACCACTGCTTAT	14580	0.4364348243858706	No Hit
GTACATGATAAGCAGTGGTATCAAC	14536	0.4351177371243495	No Hit
ATCATGTACTCTGCGTTGATACCAC	13757	0.41179930583514557	No Hit
CTGCTTATGTACTCTGCGTTGATAC	13001	0.38916935197810043	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	12779	0.3825240480676983	No Hit
GTATCAACGCAGAGTACATAAGCAG	12529	0.37504059771814635	No Hit
GTGGTATCAACGCAGAGTACATGGG	11279	0.3376233459703865	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10795	0.3231353860936539	No Hit
TATCAACGCAGAGTACATAAGCAGT	10233	0.30631258970786107	No Hit
GGTATCAACGCAGAGTACATAAGCA	9670	0.28945985952067005	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9483	0.2838622386592052	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8708	0.2606635425755941	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8576	0.2567122807910306	No Hit
GTACTGGTTCACTATCGGTCAGTCA	8014	0.23988948440523783	No Hit
GTGGTATCAACGCAGAGTACATAAG	7236	0.2166009869174321	No Hit
GCAGTGGTATCAACGCAGAGTACAT	6875	0.20579488461267906	No Hit
GTACTCTGCGTTGATACCACTGCTT	6353	0.19016944028281457	No Hit
ATACAGGGTGACAGCCCCGTACACA	5887	0.17622028883124968	No Hit
GTACATGGGATAAGCAGTGGTATCA	5853	0.17520253958371063	No Hit
ACATAAGCAGTGGTATCAACGCAGA	5792	0.17337657769841994	No Hit
GGATACCACGTGTCCCGCCCTACTC	5512	0.16499511330692176	No Hit
GAGTACATGATAAGCAGTGGTATCA	5462	0.16349842323701136	No Hit
GCTTATCCCATGTACTCTGCGTTGA	5178	0.1549972236399203	No Hit
CCACTGCTTATGTACTCTGCGTTGA	5044	0.15098609425256046	No Hit
GATACCACTGCTTATGTACTCTGCG	4924	0.14739403808477552	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4787	0.14329310729322103	No Hit
GCTTATCATGTACTCTGCGTTGATA	4612	0.13805469204853466	No Hit
ATACCACTGCTTATGTACTCTGCGT	4566	0.1366777371842171	No Hit
GTTGATACCACTGCTTATGTACTCT	4559	0.13646820057442965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4086	0.12230951251307734	No Hit
GCGTTGATACCACTGCTTATCATGT	4039	0.12090262384736156	No Hit
GATAAGCAGTGGTATCAACGCAGAG	3981	0.11916646336626549	No Hit
CAGTGGTATCAACGCAGAGTACATA	3775	0.11300010027823469	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3617	0.10827055965731784	No Hit
GTATCAACGCAGAGTACATGGGAAG	3593	0.10755214842376086	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	3561	0.10659426677901819	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	3541	0.10599559075105405	No Hit
GGTATCAACGCAGAGTACATGGGAA	3474	0.10399002605737412	No Hit
CCCATATTCAGACAGGATACCACGT	3435	0.10282260780284402	No Hit
GGATAAGCAGTGGTATCAACGCAGA	3412	0.10213413037068522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.993380139820786E-5	0.0	0.0	0.0	0.0
6	2.993380139820786E-5	0.0	0.0	0.0	0.0
7	2.993380139820786E-5	0.0	0.0	0.0	0.0
8	8.980140419462359E-5	0.0	0.0	0.0	0.0
9	8.980140419462359E-5	0.0	0.0	0.0	0.0
10	8.980140419462359E-5	0.0	0.0	0.0	0.0
11	8.980140419462359E-5	0.0	0.0	0.0	0.0
12	8.980140419462359E-5	0.0	0.0	0.0	0.0
13	1.1973520559283145E-4	0.0	0.0	2.993380139820786E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATGCG	45	6.7740673E-4	14.775227	9
TATGACG	40	0.0052711	14.2539425	4
TAACGCG	40	0.0052899867	14.24626	17
TACGCTG	110	1.8735591E-10	13.816004	7
ACGCTGT	55	1.9648347E-4	13.815177	12
CTTCGTT	255	0.0	13.781316	12
GCCACGA	65	5.468828E-5	13.150593	15
TTCGTTG	275	0.0	12.779229	13
GCTGCGT	105	2.0012521E-8	12.66448	10
AGTTGTA	360	0.0	12.40008	11
CTGTCGT	70	1.0933851E-4	12.212176	9
ACAGTGC	190	0.0	11.998108	8
GCGTTAC	95	1.0434997E-6	11.99757	13
ACGCTGC	135	3.7289283E-10	11.961077	8
TAGGACT	225	0.0	11.825495	4
TCGTTGA	315	0.0	11.759522	14
GAGCGGT	65	8.044337E-4	11.689416	18
TTAGGAC	140	6.7848305E-10	11.538906	3
CTCTTCG	315	0.0	11.45834	10
CCCGTAC	1510	0.0	11.447327	16
>>END_MODULE