FastQCFastQC Report
Wed 25 May 2016
SRR1295340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340705
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA948372.8388319232018393No Hit
GCTTATGTACTCTGCGTTGATACCA606731.8161735322334658No Hit
GTATCAACGCAGAGTACTTTTTTTT466161.3953940859788576No Hit
CTTATGTACTCTGCGTTGATACCAC397221.1890304591396128No Hit
GGTATCAACGCAGAGTACTTTTTTT307000.9189677029249814No Hit
TATCAACGCAGAGTACTTTTTTTTT292050.8742166698346607No Hit
GAGTACATAAGCAGTGGTATCAACG260210.7789074461827669No Hit
CCCATGTACTCTGCGTTGATACCAC241040.7215243489024024No Hit
TATGTACTCTGCGTTGATACCACTG217710.6516887902403834No Hit
GCGTTGATACCACTGCTTATGTACT206920.619390218531717No Hit
GTACTTTTTTTTTTTTTTTTTTTTT196140.5871215806244491No Hit
CATAAGCAGTGGTATCAACGCAGAG182470.5462020741130988No Hit
CATGTACTCTGCGTTGATACCACTG170890.5115387320939742No Hit
ACTCTGCGTTGATACCACTGCTTAT168440.5042049507514133No Hit
ACGCAGAGTACTTTTTTTTTTTTTT154160.46145948235477247No Hit
ATCATGTACTCTGCGTTGATACCAC153490.45945391766109245No Hit
ACGCAGAGTACATAAGCAGTGGTAT150230.44969549840527673No Hit
CTGCTTATGTACTCTGCGTTGATAC148580.4447564211745724No Hit
GTACATGATAAGCAGTGGTATCAAC141730.4242517672168No Hit
GTATCAACGCAGAGTACATAAGCAG133460.39949651346048215No Hit
GTGGTATCAACGCAGAGTACATGGG113340.33926970504728793No Hit
TATCAACGCAGAGTACATAAGCAGT112940.3380723529913596No Hit
GGTATCAACGCAGAGTACATAAGCA99800.29873933795411445No Hit
AAGCAGTGGTATCAACGCAGAGTAC99050.2964943028492489No Hit
GAGTACTTTTTTTTTTTTTTTTTTT86220.2580892356553482No Hit
GTACTGGTTCACTATCGGTCAGTCA85960.2573109568189948No Hit
GTACATGGGAAGCAGTGGTATCAAC82260.2462354503016579No Hit
GTGGTATCAACGCAGAGTACATAAG76820.2299514623410328No Hit
GCAGTGGTATCAACGCAGAGTACAT74460.22288708521105574No Hit
ACATAAGCAGTGGTATCAACGCAGA70600.2113326378713475No Hit
ATACAGGGTGACAGCCCCGTACACA66710.19968838912744466No Hit
GTACTCTGCGTTGATACCACTGCTT64780.19391116545759052No Hit
GCAGAGTACTTTTTTTTTTTTTTTT60790.18196757869970562No Hit
GGATACCACGTGTCCCGCCCTACTC60770.18190771109690917No Hit
GAGTACATGATAAGCAGTGGTATCA59640.1785251915389117No Hit
CCACTGCTTATGTACTCTGCGTTGA58390.17478346636413572No Hit
GTACATGGGATAAGCAGTGGTATCA57400.17182002002571314No Hit
GATACCACTGCTTATGTACTCTGCG56120.16798849344674252No Hit
GCTTATCCCATGTACTCTGCGTTGA55690.1667013399866196No Hit
GCTTATCATGTACTCTGCGTTGATA53320.15960702905524432No Hit
ATACCACTGCTTATGTACTCTGCGT50590.15143510127353357No Hit
GTTGATACCACTGCTTATGTACTCT46540.1393119117072594No Hit
GCGTTGATACCACTGCTTATCATGT45160.1351810471143067No Hit
ATCAACGCAGAGTACTTTTTTTTTT44760.1339836950583784No Hit
CAGTGGTATCAACGCAGAGTACATA44760.1339836950583784No Hit
GATAAGCAGTGGTATCAACGCAGAG43920.13146925574092894No Hit
ACCCTGTATCGCGCGCCTTTCCAGA42190.12629070809903897No Hit
GTGGTATCAACGCAGAGTACTTTTT42050.12587163487946407No Hit
GGATAAGCAGTGGTATCAACGCAGA40250.12048355062778665No Hit
CCCATATTCAGACAGGATACCACGT40070.11994474220261891No Hit
GAGTACATGGGAAGCAGTGGTATCA39640.11865758874249598No Hit
TACCACTGCTTATGTACTCTGCGTT38970.11665202404881606No Hit
ACGCAGAGTACATGATAAGCAGTGG38540.11536487058869312No Hit
TGATACCACTGCTTATGTACTCTGC37640.1126708284628544No Hit
GTATCAACGCAGAGTACATGGGAAG37610.11258102705865977No Hit
CCCCATGTACTCTGCGTTGATACCA36270.10856989767129993No Hit
GGTATCAACGCAGAGTACATGGGAA35900.10746234701956624No Hit
GCAGAGTACATAAGCAGTGGTATCA35060.10494790770211677No Hit
GGGATAAGCAGTGGTATCAACGCAG34820.10422949646855977No Hit
CAGTGGTATCAACGCAGAGTACATG34640.10369068804339204No Hit
GCGTTGATACCACTGCTTCCCATGT34430.10306207821402967No Hit
GTATTTAGCCTTGGAGGATGGTCCC34330.10276274020004761No Hit
TATCAACGCAGAGTACATGATAAGC33930.10156538814411929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT350.002176439416.27950510
TCTTACG602.5510075E-514.2612162
GCTCTCG400.00529468614.24435418
AGTCCCG551.9675685E-413.81291311
TTCGTTT655.4764732E-513.14863417
TTTACGC751.4678657E-512.6766372
GGACCCT604.1068863E-412.6620276
ATAGGGC1001.4296165E-712.359723
AAGTCCC1001.4515354E-712.34529110
TATCCGT853.963347E-612.2896156
ACGTTAC550.003078495612.08629912
TGCGTTT550.003078495612.08629912
GCGCTTA550.00307884112.08611816
AGGGTGA12800.011.5872375
TAGGACC1406.730261E-1011.5447944
GTCTAAA3200.011.3024931
ACAGGGT14900.011.2941173
CCCTAGG855.2257685E-511.1975161
TCGGCAC855.284357E-511.1852673
GATCGTC600.00589541811.0789427
Produced by FastQC (version 0.11.5)