FastQCFastQC Report
Wed 25 May 2016
SRR1295339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG54410.13994057219516373No Hit
GTATCAACGCAGAGTACTTTTTTTT49150.12641204049609073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG600.00584632811.0914691
GGTATCA33450.09.9759271
GCGTCAG1804.136382E-99.5069731
TATCTCG900.00111142349.502695
TATACCG1002.7493987E-49.502695
CGTCAGA1804.1727617E-99.5020782
CTAGACT2950.09.3416284
GTATTAG5200.09.1413211
ACTAGAC3250.09.0641053
TAGACAG5900.09.0195035
GTCTAAC1804.1743988E-88.9788081
GTCCTAC2553.6379788E-128.947741
TGGTATC15950.08.876552
ACCGTGT2054.1582098E-98.8035188
CGCAGAT1304.3800286E-58.7678788
TAGCACC2950.08.6973784
GTGGTAT16750.08.5137071
GTCTTAA4700.08.4955931
GTTATAC3700.08.4753723
ACGCAGG1804.04787E-78.4431418
Produced by FastQC (version 0.11.5)