FastQCFastQC Report
Wed 25 May 2016
SRR1295339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT128670.33093463378701926No Hit
GGTATCAACGCAGAGTACTTTTTTT105330.27090498932763457No Hit
TATCAACGCAGAGTACTTTTTTTTT76380.19644662569870622No Hit
GTGGTATCAACGCAGAGTACATGGG59100.15200308429947026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49510.12733794760857484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC508.743908E-515.194988
AGCGTCA3350.011.0672923
GGTATCA43600.010.7387381
GCAGCGT3550.010.1866151
AGGACCG856.5569795E-410.06559855
GAACCGC856.616972E-410.0556316
GTGGTAT19850.09.5883551
TCTAGAC4000.09.2688573
CTTAGAC3100.09.1998593
TGGTATC20100.09.1281512
CGAAAAA1054.5101778E-49.0446314
TACACGG850.0073979828.9471985
CAGCGTC4300.08.8432742
GACTGCG1201.7713165E-48.7054587
GTATTAG5450.08.5560681
GTCCTAC2605.820766E-118.4183921
TGCACTG6650.08.2912945
CTAGCAC3450.08.2665393
CTACACT5900.08.2175014
GTCTAGC2105.6470526E-88.1569521
Produced by FastQC (version 0.11.5)