FastQCFastQC Report
Wed 25 May 2016
SRR1295338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616315
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA615123.8056938158712876No Hit
GCTTATGTACTCTGCGTTGATACCA359852.226360579466255No Hit
CTTATGTACTCTGCGTTGATACCAC236721.4645660035327273No Hit
GAGTACATAAGCAGTGGTATCAACG164671.0187989346136117No Hit
TATGTACTCTGCGTTGATACCACTG151280.9359561719095598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA132010.816734361804475No Hit
CCCATGTACTCTGCGTTGATACCAC127390.7881508245608065No Hit
GCGTTGATACCACTGCTTATGTACT124630.7710749451684851No Hit
GTACATGATAAGCAGTGGTATCAAC111380.6890983502596957No Hit
CATAAGCAGTGGTATCAACGCAGAG107910.6676297627628278No Hit
ATCATGTACTCTGCGTTGATACCAC105190.6508013598834386No Hit
ACTCTGCGTTGATACCACTGCTTAT102020.6311888462335621No Hit
CATGTACTCTGCGTTGATACCACTG98230.6077404466332368No Hit
GTATCAACGCAGAGTACTTTTTTTT90490.5598537413808571No Hit
CTGCTTATGTACTCTGCGTTGATAC90230.5582451440467978No Hit
ACGCAGAGTACATAAGCAGTGGTAT90150.5577501910209335No Hit
GTATCAACGCAGAGTACATAAGCAG83270.5151842307965959No Hit
TATCAACGCAGAGTACATAAGCAGT69560.43036165598908627No Hit
GGTATCAACGCAGAGTACATAAGCA67970.4205244646000315No Hit
GGTATCAACGCAGAGTACTTTTTTT61070.377834766119228No Hit
AAGCAGTGGTATCAACGCAGAGTAC58830.36397608139502513No Hit
GTGGTATCAACGCAGAGTACATGGG57000.3526540309283772No Hit
GTACATGGGAAGCAGTGGTATCAAC54850.33935216835827176No Hit
TATCAACGCAGAGTACTTTTTTTTT54250.33564002066428883No Hit
GTGGTATCAACGCAGAGTACATAAG50070.3097787250628745No Hit
GTACTGGTTCACTATCGGTCAGTCA48360.29919910413502315No Hit
GCAGTGGTATCAACGCAGAGTACAT45550.2818138791015365No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45260.2800196743827781No Hit
ACATAAGCAGTGGTATCAACGCAGA44430.2748845367394351No Hit
GAGTACATGATAAGCAGTGGTATCA44300.27408023807240545No Hit
GTACTCTGCGTTGATACCACTGCTT41810.25867482514237633No Hit
CCACTGCTTATGTACTCTGCGTTGA36230.22415185158833517No Hit
GCTTATCATGTACTCTGCGTTGATA36130.2235331603060047No Hit
GATACCACTGCTTATGTACTCTGCG34810.2153664353792423No Hit
ATACAGGGTGACAGCCCCGTACACA32690.20225018019383598No Hit
GGATACCACGTGTCCCGCCCTACTC32270.19965167680804793No Hit
GCGTTGATACCACTGCTTATCATGT32250.19952793855158185No Hit
ATACCACTGCTTATGTACTCTGCGT31650.1958157908575989No Hit
GTTGATACCACTGCTTATGTACTCT31570.19532083783173454No Hit
GATAAGCAGTGGTATCAACGCAGAG28990.17935860274760798No Hit
GTACATGGGATAAGCAGTGGTATCA28860.17855430408057835No Hit
ACGCAGAGTACATGATAAGCAGTGG27730.17156309259024385No Hit
GCTTATCCCATGTACTCTGCGTTGA26240.16234459248351962No Hit
GTTAATGATAGTGTGTCGAAACACA26210.16215898509882046No Hit
GGTATCAACGCAGAGTACATGGGGG25410.1572094548401766No Hit
CAGTGGTATCAACGCAGAGTACATA25000.15467282058262158No Hit
TATCAACGCAGAGTACATGATAAGC24990.15461095145438852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24440.15120814940157085No Hit
TACCACTGCTTATGTACTCTGCGTT24310.15040385073454124No Hit
GAACAAAAAAAAAAAAAAAAAAAAA24100.1491045990416472No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA23650.14632048827116No Hit
GAGTACATGGGAAGCAGTGGTATCA23070.1427320788336432No Hit
TGATACCACTGCTTATGTACTCTGC22830.14124721975605No Hit
GTATCAACGCAGAGTACATGATAAG22810.14112348149958395No Hit
ATCATTAACTGAATCCATAGGTTAA22770.14087600498665173No Hit
CCCCATGTACTCTGCGTTGATACCA22500.13920553852435943No Hit
GCAGAGTACATAAGCAGTGGTATCA21500.13301862570105455No Hit
GGTATCAACGCAGAGTACATGATAA21110.13060572969996567No Hit
ATGATAAGCAGTGGTATCAACGCAG20620.1275741424165463No Hit
GGTATCAACGCAGAGTACATGGGAA19860.12287208867083459No Hit
GTATCAACGCAGAGTACATGGGAAG19750.12219152826027103No Hit
GTATCAACGCAGAGTACATGGGGGT19690.12182031349087276No Hit
GCGTTGATACCACTGCTTCCCATGT19290.1193455483615508No Hit
GTATTTAGCCTTGGAGGATGGTCCC18990.11748947451455935No Hit
CCCATATTCAGACAGGATACCACGT18870.11674704497576277No Hit
GGTTAATGAGGCGAACCGGGGGAAC18820.11643769933459754No Hit
ACTTAGATGTTTCAGTTCCCCCGGT18680.11557153153933485No Hit
CTGCTTATCATGTACTCTGCGTTGA18360.1135917194358773No Hit
CAGTGGTATCAACGCAGAGTACATG18250.11291115902531375No Hit
ATCAACGCAGAGTACATAAGCAGTG17840.11037452476775876No Hit
GGATAAGCAGTGGTATCAACGCAGA17620.1090134039466317No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17440.1078997596384368No Hit
TCTAAGTACCCCGAGGAAAAGAAAT16690.10325957502095816No Hit
GTACATGGGGGTTAAGCGACTAAGC16620.10282649112332681No Hit
TATCAACGCAGAGTACATGGGAAGC16460.10183658507159805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAG402.7434694E-416.6390461
CCCTATG402.7545146E-416.6302852
AGTTGTA2900.015.0662911
AGATTAC1101.2732926E-1114.67921211
GTACAAA5450.014.4801131
ATATAGC400.00524972514.2620391
TAGAACC400.005268101614.254534
TCAACTA400.005268101614.254535
ATCACGG400.00528436114.24791056
TTGTATC3050.014.01390513
ACTCTTC1850.013.8628319
TACATCC551.9626664E-413.8161567
TGTATCA3050.013.39106514
CCTAGAG500.00149600413.3042292
GCGTCCG500.001501600113.29763813
GTACTAG655.4097065E-513.164961
GTATATC655.4097065E-513.164961
CTTACTC801.9924173E-613.0666523
GCAGGAC957.356721E-813.0041323
AGCAGTT3100.012.8690818
Produced by FastQC (version 0.11.5)