##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295338_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616315
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.698154134559168	31.0	30.0	33.0	25.0	34.0
2	29.40845565375561	31.0	30.0	34.0	22.0	34.0
3	29.815152368195555	31.0	30.0	34.0	25.0	34.0
4	32.667011690171776	35.0	33.0	37.0	20.0	37.0
5	33.49022498708482	35.0	33.0	37.0	27.0	37.0
6	33.478940676786394	36.0	35.0	37.0	27.0	37.0
7	33.55082703557166	36.0	35.0	37.0	27.0	37.0
8	33.43263039692139	36.0	35.0	37.0	26.0	37.0
9	34.187006864379775	38.0	34.0	39.0	22.0	39.0
10	34.10684489100206	38.0	33.0	39.0	22.0	39.0
11	34.62062036174879	38.0	34.0	39.0	26.0	39.0
12	34.63426807274572	38.0	34.0	39.0	25.0	39.0
13	34.36342792091888	38.0	34.0	39.0	24.0	39.0
14	34.54146004955717	38.0	32.0	40.0	18.0	41.0
15	35.090553512155736	39.0	33.0	41.0	24.0	41.0
16	35.110757494671525	38.0	34.0	40.0	24.0	41.0
17	35.17694261329011	39.0	34.0	40.0	24.0	41.0
18	35.10864899478134	39.0	33.0	40.0	23.0	41.0
19	35.16284758849606	39.0	34.0	41.0	23.0	41.0
20	35.18229429288227	39.0	34.0	41.0	24.0	41.0
21	35.19119416697859	39.0	33.0	41.0	24.0	41.0
22	34.96857976322685	39.0	33.0	40.0	23.0	41.0
23	34.65683112512103	38.0	32.0	40.0	21.0	41.0
24	34.978869836634566	39.0	33.0	40.0	24.0	41.0
25	33.8457429399591	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4573838901367502
1101	2	0.6091926819297875
1101	3	0.39673395610287443
1101	4	0.09232260345621057
1101	5	0.16555067365639076
1101	6	0.13536741577639333
1101	7	0.1198124208038962
1101	8	0.04674761477264866
1101	9	0.11305785894192866
1101	10	0.5448998166429675
1101	11	0.46713524166404596
1101	12	0.3469086843277225
1101	13	0.3083589811618239
1101	14	0.9530748750495732
1101	15	0.620785962408462
1101	16	0.4412223462408136
1101	17	0.8038837483944334
1101	18	0.6659300364664773
1101	19	0.6156788550794587
1101	20	0.6442826905204484
1101	21	0.5086939105961221
1101	22	0.5400073507111003
1101	23	-1.75445798635441
1101	24	0.0230245423837232
1101	25	0.44726065278246807
1102	1	0.32589412354851177
1102	2	0.4825205213482313
1102	3	0.15375068069451459
1102	4	0.28056562558713694
1102	5	0.0902349925104744
1102	6	0.050408241933794784
1102	7	0.04696645345686079
1102	8	0.1643293367566656
1102	9	0.33073727706468503
1102	10	0.6755186782878795
1102	11	0.3349145455292941
1102	12	0.2700500889386319
1102	13	0.23018296090599932
1102	14	0.8696917381834126
1102	15	0.4269206747940544
1102	16	0.3002016724002061
1102	17	0.5496962307283084
1102	18	0.6036702857628171
1102	19	0.7078288469182112
1102	20	0.8348011488801532
1102	21	0.6349375458161006
1102	22	0.48393008556600847
1102	23	0.6760141320275181
1102	24	0.6825212520064312
1102	25	0.3919296045992411
1103	1	-0.17990058715179202
1103	2	-1.390523950873078
1103	3	-0.2828091364167662
1103	4	0.139066168332441
1103	5	0.040240255495241684
1103	6	0.16602434008846245
1103	7	0.10064330619196227
1103	8	0.1969310603841805
1103	9	0.8832143337404474
1103	10	0.935798603290344
1103	11	0.5480904279015846
1103	12	0.3689445332003345
1103	13	0.3635058779153084
1103	14	-0.8008172911248366
1103	15	-0.037805156593073264
1103	16	-0.006975558611927113
1103	17	0.5208912572077011
1103	18	0.7205971960618243
1103	19	0.4952292613782632
1103	20	0.7997330590146916
1103	21	0.7369085336125494
1103	22	0.2761610977037634
1103	23	1.1167233192253079
1103	24	0.9754844769003483
1103	25	0.80077701093456
1104	1	0.1495341791045206
1104	2	0.15135961198411962
1104	3	-0.41306370213835564
1104	4	-0.4110814800586198
1104	5	-0.26752181188202684
1104	6	-0.11400033838280876
1104	7	-0.2000586198462102
1104	8	-0.17542326032420164
1104	9	-2.274407988897515
1104	10	-0.5149078196531391
1104	11	-0.72905657184495
1104	12	-0.1285637724475066
1104	13	0.055998635172358036
1104	14	-1.533717808677757
1104	15	-0.5403363692040273
1104	16	-0.3069773982550146
1104	17	0.15149773983738157
1104	18	0.09758332924107549
1104	19	0.23865219668550708
1104	20	0.21273940373927758
1104	21	0.3371718069780627
1104	22	0.4079228080540034
1104	23	0.7282806384156473
1104	24	0.5770460613372421
1104	25	0.662933917499771
1105	1	-0.14061418216771315
1105	2	-0.06703588959431883
1105	3	-1.354511397072514
1105	4	-0.6383450596601854
1105	5	-0.31754253368289653
1105	6	-0.17082804385694317
1105	7	-0.10854125531817971
1105	8	-0.10914794860180166
1105	9	0.3410639479854112
1105	10	0.5133795704061939
1105	11	-0.13196427540893296
1105	12	-0.04145358533732235
1105	13	-0.009812098891039511
1105	14	0.67705428732814
1105	15	0.3185844939826268
1105	16	-0.1183100914580919
1105	17	0.39492790566295355
1105	18	0.2795954416879738
1105	19	0.21703944365356165
1105	20	-0.4913650477810876
1105	21	0.08898995313582958
1105	22	0.047336355506928385
1105	23	0.3434602529389821
1105	24	0.1463116227274952
1105	25	0.3055196912586382
1106	1	-1.5902194559187741
1106	2	-0.9708410790487108
1106	3	-1.0365765707094425
1106	4	-1.3301157158955341
1106	5	-0.6696459249662396
1106	6	-0.6796379927580105
1106	7	-0.6899306348852363
1106	8	-0.7121902058581853
1106	9	-0.3495369735950078
1106	10	-2.17270342866086
1106	11	-1.265458940566468
1106	12	-0.94291456971402
1106	13	-1.024152951045103
1106	14	-0.22133602518518103
1106	15	-0.8645410180722735
1106	16	-1.416054445238892
1106	17	-0.772444548269867
1106	18	-0.5938695775629199
1106	19	-0.4497831876337628
1106	20	-0.12986021019424498
1106	21	-0.07736809932700339
1106	22	-0.18897277647418065
1106	23	-0.37418095581839594
1106	24	-0.9426980808607937
1106	25	-1.7434888757453706
1107	1	0.01768309191903228
1107	2	0.30021518555149385
1107	3	-0.14798807212813614
1107	4	0.07745005087176793
1107	5	-0.12286968637846485
1107	6	8.194409484332255E-4
1107	7	-0.054741190991798305
1107	8	-0.25429147269269947
1107	9	0.3133270097221583
1107	10	-0.052136350372883555
1107	11	-0.025918590415876963
1107	12	-0.051211300358190215
1107	13	-0.05090488472066568
1107	14	-1.0643891435889756
1107	15	-0.3415944925121508
1107	16	-0.24900703794450862
1107	17	-0.11571346813631322
1107	18	0.21872610713442953
1107	19	0.13553377530578814
1107	20	0.2558859942067926
1107	21	0.3653533242360467
1107	22	-0.23601985679665205
1107	23	0.5833324954428036
1107	24	0.2535375641639632
1107	25	0.10478889052783558
1108	1	-0.04148483825658644
1108	2	0.305433704507152
1108	3	0.09337981628359415
1108	4	-0.4188087520783341
1108	5	-0.19351723850010671
1108	6	-0.1628173766432397
1108	7	-0.1811816668580235
1108	8	-0.10601090409853953
1108	9	0.3652979323711989
1108	10	0.34330457642228396
1108	11	0.18814918208592957
1108	12	0.012422944820045245
1108	13	0.03363233810178201
1108	14	0.25246288816368434
1108	15	0.14972376345206584
1108	16	-0.032855610557689374
1108	17	0.26634379681318876
1108	18	0.3069719935427031
1108	19	0.2748065086312508
1108	20	0.34276214611840317
1108	21	0.3549262733416043
1108	22	0.4290308802754552
1108	23	0.17205185724215966
1108	24	0.2632115031947393
1108	25	-0.5313792424144879
1109	1	-0.8429533163849356
1109	2	-0.39996598100876213
1109	3	-0.676290913250714
1109	4	-0.6432677050276396
1109	5	-0.8437037094322051
1109	6	-1.0689290278722652
1109	7	-0.7999913164552268
1109	8	-0.7971106293157177
1109	9	-0.716808517053444
1109	10	-1.1809840049293783
1109	11	-1.0866389475465397
1109	12	-1.0313644314260912
1109	13	-0.9653526223063551
1109	14	-0.3532092356042469
1109	15	-1.185573167670917
1109	16	-1.3873721772417582
1109	17	-1.2026346613394807
1109	18	-2.06685689710374
1109	19	-2.0340224914281464
1109	20	-1.6456514911120124
1109	21	-1.4914160516523793
1109	22	-1.3653174718091705
1109	23	-0.9740299086203734
1109	24	-2.0491978012827516
1109	25	-1.2085753948298574
1110	1	0.05834591836386949
1110	2	0.24372336451487797
1110	3	0.10038127016204612
1110	4	0.23111608116929716
1110	5	0.20249772357468743
1110	6	0.17779470691222343
1110	7	-0.017810385284896313
1110	8	0.08196023361408322
1110	9	0.40272464751502923
1110	10	0.26906455338132673
1110	11	0.27504122051951896
1110	12	0.15871104549039927
1110	13	0.08239901424763474
1110	14	-2.3618892704348866
1110	15	-0.503441931416738
1110	16	-0.1706819786325866
1110	17	-0.12452666108942623
1110	18	-2.6205995494230763
1110	19	-0.29161769949956096
1110	20	-2.031200847256599
1110	21	-0.1150038983091477
1110	22	-1.6369570188592704
1110	23	-0.5066020215004485
1110	24	0.29237518488918113
1110	25	0.23208717037498872
1111	1	-0.19909838031371052
1111	2	-1.3844804908206285
1111	3	-0.47789961116018986
1111	4	-9.8550416073806E-4
1111	5	-0.13619890068668639
1111	6	0.046144347442883316
1111	7	0.022016719436571464
1111	8	-0.057708547277698585
1111	9	-1.5996058710087695
1111	10	-0.3310412413474353
1111	11	-0.10997735027058297
1111	12	-0.15431319596787318
1111	13	0.0032661185684261795
1111	14	0.7439505337785732
1111	15	0.34667866750339726
1111	16	0.185741965092042
1111	17	-0.11211912836332516
1111	18	0.08239434417941993
1111	19	-0.251244828320182
1111	20	0.3607942539554614
1111	21	-0.4945323973350213
1111	22	-0.2879229444718163
1111	23	-0.0811566657594085
1111	24	-0.6884690753122342
1111	25	-2.0528986691581856
1112	1	-0.09772013795111434
1112	2	0.2465714157837411
1112	3	-0.1350750953437796
1112	4	0.2700528255929555
1112	5	0.02209537472797507
1112	6	-0.026748769873186973
1112	7	-0.025745952243667602
1112	8	-0.12480721833895814
1112	9	-0.28924380847958275
1112	10	0.1960461006112979
1112	11	0.1028186055899667
1112	12	-0.11350131836962163
1112	13	-0.08952662945053902
1112	14	0.3778160789055107
1112	15	0.01977843081212427
1112	16	0.015005725913567858
1112	17	-3.4818341669844983
1112	18	-0.9088058347368175
1112	19	-1.265779231009347
1112	20	-0.3084524284981569
1112	21	-0.45502455252452023
1112	22	-1.7920999156707111
1112	23	-1.551894147411744
1112	24	-0.5187030952629783
1112	25	-0.04290776745537528
1113	1	-0.3256308308405913
1113	2	-0.20179005100543534
1113	3	-0.023638140489133264
1113	4	-0.2797474851121322
1113	5	-0.21964667154814066
1113	6	-0.09023514214241146
1113	7	-0.2326853893676315
1113	8	-0.24109205024728908
1113	9	0.18639800409878404
1113	10	0.40875413147631434
1113	11	0.0998830686015566
1113	12	-0.06592721017511138
1113	13	-0.5197849492134878
1113	14	-1.45959702026272
1113	15	-1.5364192688142921
1113	16	-0.387197893485002
1113	17	-0.16624775259937508
1113	18	-0.4048075001075375
1113	19	0.044357803528363604
1113	20	0.4644259496705061
1113	21	-1.4766706259260545
1113	22	-1.9187472921499165
1113	23	-0.6252269170591873
1113	24	-2.294687721622175
1113	25	-0.6852969643795745
1114	1	0.030734261169012456
1114	2	0.24192102382982128
1114	3	0.04178400544771321
1114	4	0.49994778350266955
1114	5	0.10432390927472568
1114	6	0.15930941692862888
1114	7	0.08791355512418164
1114	8	0.09791548189626553
1114	9	0.5050319720047014
1114	10	0.3796459156179779
1114	11	0.31431700573137533
1114	12	0.33555193608416545
1114	13	0.3482494581452542
1114	14	1.1008292100411836
1114	15	0.49987012572596257
1114	16	0.5664816771857488
1114	17	-0.1937162210990806
1114	18	0.2118431162278469
1114	19	-0.3016676926295574
1114	20	-1.4815587961586516
1114	21	-0.5526225181361042
1114	22	-1.264240207113204
1114	23	-2.557712316413742
1114	24	-1.7344007038288467
1114	25	-1.3479701166068594
1115	1	0.21487537332184203
1115	2	0.10368978113135086
1115	3	0.27322843066731295
1115	4	0.5615361402210155
1115	5	0.48878357264321437
1115	6	0.2415865685576648
1115	7	0.3636001168231928
1115	8	0.3552516157977621
1115	9	0.5873266942481621
1115	10	0.895569452596078
1115	11	0.4681347246900671
1115	12	0.44176589788216347
1115	13	0.3397176506387112
1115	14	1.0373354476093013
1115	15	0.4033780787762069
1115	16	0.571482998335
1115	17	0.5934528041878551
1115	18	0.7214724835650159
1115	19	0.19718002379413946
1115	20	0.7498288934128539
1115	21	-0.3483627842169028
1115	22	0.8605528687234454
1115	23	0.8632008726796059
1115	24	0.8503013267172008
1115	25	1.0441495298884789
1116	1	0.2949466681472366
1116	2	0.5040481659159646
1116	3	0.4082647058675235
1116	4	0.6688755221505644
1116	5	0.6497856852846695
1116	6	0.5374693742765402
1116	7	0.31121561607803727
1116	8	0.398138783275094
1116	9	0.6507979224734868
1116	10	0.6188966718249347
1116	11	0.4537922365770868
1116	12	0.4619727402201548
1116	13	0.013034770677144536
1116	14	1.0788900678524413
1116	15	0.6582551688992027
1116	16	0.7649613778789544
1116	17	0.9067523461759208
1116	18	1.023521254466246
1116	19	1.0586715377446723
1116	20	1.0569488226639265
1116	21	0.40735008215170154
1116	22	0.45491015078564345
1116	23	-0.6287364249954024
1116	24	-0.06967732850735331
1116	25	-0.42961250043092747
1201	1	0.5487912566353152
1201	2	0.7782738316190141
1201	3	0.626615605741236
1201	4	0.4428504082822755
1201	5	0.46639070446720865
1201	6	0.3583481202589809
1201	7	0.27364027746576625
1201	8	0.32649483041446814
1201	9	0.6645366329038822
1201	10	0.9132092425250207
1201	11	0.3983699513108334
1201	12	0.59071333598623
1201	13	0.5196020232941692
1201	14	0.9480375968656674
1201	15	0.7046844029741024
1201	16	0.5815496385221195
1201	17	0.5284190427685473
1201	18	0.5264815307427355
1201	19	0.9761949847368143
1201	20	0.8308518864840408
1201	21	0.821918340281087
1201	22	0.8141165740943777
1201	23	0.5308075631778593
1201	24	0.7423089175199706
1201	25	0.9497323005314868
1202	1	0.29463636049765896
1202	2	0.668123410294438
1202	3	0.3299660817937422
1202	4	0.20261169792983935
1202	5	0.10619642031657861
1202	6	-0.04689368866006305
1202	7	-0.11716140622289828
1202	8	0.10453846789022236
1202	9	0.5286166000671244
1202	10	-0.4138545235613691
1202	11	0.08933903807874799
1202	12	0.010714784666724597
1202	13	0.210424632367733
1202	14	0.6949128735233998
1202	15	0.42977152500933613
1202	16	0.2543579056588072
1202	17	-1.7956161762638914
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2210	18	0.7037244025003488
2210	19	0.898956304464896
2210	20	1.0012894931146548
2210	21	1.001141145689033
2210	22	1.2872302114811873
2210	23	1.5688407938974152
2210	24	1.2996395931408955
2210	25	1.1441348042456099
2211	1	0.24684639855129475
2211	2	-0.6808584744358903
2211	3	0.012528727425959119
2211	4	0.4199137176997141
2211	5	0.4174708094668276
2211	6	0.4419493208770291
2211	7	0.3516280129874687
2211	8	0.46914633531045524
2211	9	0.9221915065635713
2211	10	1.0161001552654838
2211	11	0.4357257285460818
2211	12	0.44117663824602005
2211	13	0.3258646561580534
2211	14	-0.5352404202784626
2211	15	0.23117844578618474
2211	16	0.48435886068780576
2211	17	0.529536725432564
2211	18	0.4691952760766469
2211	19	0.8487158313934913
2211	20	0.8401222885105852
2211	21	0.8159792020933097
2211	22	0.16922145701233404
2211	23	0.7010830860364408
2211	24	0.22587884231796096
2211	25	0.7802729054151811
2212	1	-0.3277156679239077
2212	2	-1.4450823734703988
2212	3	-0.6587464703658839
2212	4	-0.21623616221496178
2212	5	-7.876083795821387E-5
2212	6	-0.12024427623281753
2212	7	-0.2490306754040077
2212	8	-0.2765163173154619
2212	9	-2.5366150590657917
2212	10	-0.4215801392171912
2212	11	-0.3057388606184972
2212	12	-0.27430368276140626
2212	13	-0.40102217754506597
2212	14	0.21084848166044878
2212	15	0.0012599428015747094
2212	16	0.017882413568493405
2212	17	-0.008204581774464259
2212	18	-0.16863165743598785
2212	19	-0.8618378641475175
2212	20	-0.1912517747978626
2212	21	-0.1032207812273711
2212	22	-1.774823040867716
2212	23	-0.716114654842201
2212	24	-0.152558444304006
2212	25	-0.7542612521545919
2213	1	0.09348908844230408
2213	2	0.3744579684662561
2213	3	0.11117182160920436
2213	4	0.4735540323738405
2213	5	0.35515505723058993
2213	6	0.14276043217530798
2213	7	0.2117260538546759
2213	8	0.3150225545042318
2213	9	0.6417895726990466
2213	10	0.7569656775605154
2213	11	0.1130836787844487
2213	12	0.10232078527363342
2213	13	0.17006895850126114
2213	14	-1.8119489761129017
2213	15	-0.33171426516170754
2213	16	-0.23442640959839878
2213	17	-2.0455900370289015
2213	18	-0.9440208686599689
2213	19	-0.43142078569122333
2213	20	0.28732003795047234
2213	21	-0.20943224948427286
2213	22	0.34226695673438456
2213	23	0.8612754617586873
2213	24	0.6782313177656292
2213	25	-0.8837054986412127
2214	1	-0.03653860643358442
2214	2	-0.09813047523776319
2214	3	-0.007786623166907702
2214	4	0.32666917266966067
2214	5	0.3909180162602439
2214	6	0.3101882775936673
2214	7	0.3381789262578607
2214	8	0.15581841453003875
2214	9	0.5510355300273773
2214	10	0.5092885052892981
2214	11	0.03926734846215396
2214	12	-0.05611899521051811
2214	13	0.05603303374555679
2214	14	0.793349507180082
2214	15	0.3589501368192529
2214	16	0.45499399626007175
2214	17	0.2560353109111304
2214	18	0.21082865341538337
2214	19	-0.7875321815096044
2214	20	0.16962186719309358
2214	21	0.48434656309371604
2214	22	0.7009809917049168
2214	23	1.257181121807733
2214	24	0.9283445614058863
2214	25	0.9849381933652737
2215	1	-0.25007673701285427
2215	2	0.11227126121679021
2215	3	0.17626418119646914
2215	4	0.47078256661783513
2215	5	0.38733963399610616
2215	6	0.0816979084051539
2215	7	0.11569208817638099
2215	8	0.11644407329628592
2215	9	0.22453372644199732
2215	10	0.5438969386544343
2215	11	0.1921704075648023
2215	12	0.12944602777865555
2215	13	-0.07344789973000587
2215	14	0.913734686013818
2215	15	0.5522574316133486
2215	16	0.5483819129647856
2215	17	0.6124549981240861
2215	18	0.6458715673308504
2215	19	-0.2863519235900469
2215	20	-1.4594349298351403
2215	21	-1.8379343384322624
2215	22	-0.11820267652286987
2215	23	0.4099674745913262
2215	24	0.5737223208505924
2215	25	-0.1738436466798987
2216	1	0.09537256093199886
2216	2	-0.20344673646541622
2216	3	-0.06205218829531489
2216	4	0.5147419302468919
2216	5	0.37463119782137966
2216	6	0.20529482682659506
2216	7	0.27189224310695437
2216	8	0.08985912822954134
2216	9	0.4764235109600392
2216	10	-1.5142041768428243
2216	11	-0.38591805655936895
2216	12	-0.04393083216603344
2216	13	0.22775987340896364
2216	14	-0.3131963079433788
2216	15	-0.3381577912115432
2216	16	0.06502404585094013
2216	17	0.3567161795851206
2216	18	0.47140706780922415
2216	19	-1.0939327685738789
2216	20	-0.5624960201672948
2216	21	-0.6309163533813518
2216	22	-0.4472451909574602
2216	23	-4.067607092019575
2216	24	-1.8587341287617676
2216	25	-1.043049324255982
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	22.0
5	32.0
6	48.0
7	171.0
8	226.0
9	525.0
10	1071.0
11	1986.0
12	3666.0
13	6110.0
14	9417.0
15	13369.0
16	17627.0
17	19561.0
18	19755.0
19	18693.0
20	17754.0
21	17235.0
22	16204.0
23	16122.0
24	16199.0
25	16261.0
26	17178.0
27	18904.0
28	21696.0
29	25852.0
30	31036.0
31	38837.0
32	51744.0
33	70333.0
34	96172.0
35	134095.0
36	192822.0
37	285026.0
38	420555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.17742994068332	19.151548549279905	12.078808217651357	19.59221329238542
2	13.317149179694848	24.44485836493431	38.42278141367736	23.81521104169348
3	20.644730465952925	32.10649539208581	29.65508875750631	17.59368538445495
4	12.958162818160886	21.962911247935867	31.65102012017142	33.427905813731826
5	10.18239283353265	46.03521829449764	31.379787610707115	12.4026012612626
6	27.88675149413423	34.18581596622743	22.96591678393253	14.961515755705804
7	22.595244018511025	40.34273118048001	18.80052131874334	18.26150348226563
8	22.241093207745	38.01630180812788	23.130890434741705	16.611714549385418
9	27.268495909673003	23.34664693456034	17.451711116516602	31.933146039250055
10	15.880531655299132	30.42456055881113	26.99288737445532	26.70202041143441
11	27.694369906825226	31.030059013991067	19.389710177933125	21.88586090125058
12	25.451863146482374	30.170497581647254	23.314950222033	21.062689049837374
13	28.112293713339366	23.83772095400889	29.253685712765048	18.796299619886696
14	27.712648814866075	27.89530563915033	22.893504448507613	21.498541097475982
15	31.038394906839756	25.80987177352619	23.18920414419645	19.962529175437606
16	22.763847768613044	27.78774841322494	28.779049618282816	20.669354199879198
17	20.16368789761616	34.15408322400178	25.396997301779834	20.28523157660222
18	20.550195260522717	25.970949195130554	34.654936594483196	18.823918949863533
19	22.45426867001658	34.20251738904428	23.206762543627317	20.136451397311813
20	21.456073128531862	30.574181747074885	28.488295821049945	19.48144930334331
21	22.722283069421497	32.37062750994697	24.322090008477357	20.584999412154176
22	21.088474945682073	30.607030296129395	21.452224049150065	26.852270709038468
23	24.38508802491146	26.53575607857358	23.835384844826272	25.243771051688686
24	20.004962997204757	30.93970824754836	21.48253761388748	27.572791141359403
25	23.656503999583816	31.728503940128867	23.035942992615198	21.579049067672116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14799.0
1	14799.0
2	12272.0
3	9745.0
4	9745.0
5	9745.0
6	13643.5
7	17542.0
8	17542.0
9	17542.0
10	16590.0
11	15638.0
12	15638.0
13	15638.0
14	16412.5
15	17187.0
16	17187.0
17	17187.0
18	20822.0
19	24457.0
20	24457.0
21	24457.0
22	29717.5
23	34978.0
24	34978.0
25	34978.0
26	43839.5
27	52701.0
28	52701.0
29	52701.0
30	59161.5
31	65622.0
32	65622.0
33	65622.0
34	72525.0
35	79428.0
36	79428.0
37	79428.0
38	110438.0
39	141448.0
40	141448.0
41	141448.0
42	270023.0
43	398598.0
44	398598.0
45	398598.0
46	328325.5
47	258053.0
48	258053.0
49	258053.0
50	213001.5
51	167950.0
52	167950.0
53	167950.0
54	137215.5
55	106481.0
56	106481.0
57	106481.0
58	91570.0
59	76659.0
60	76659.0
61	76659.0
62	69722.5
63	62786.0
64	62786.0
65	62786.0
66	49182.5
67	35579.0
68	35579.0
69	35579.0
70	28163.5
71	20748.0
72	20748.0
73	20748.0
74	15392.0
75	10036.0
76	10036.0
77	10036.0
78	7017.5
79	3999.0
80	3999.0
81	3999.0
82	2649.0
83	1299.0
84	1299.0
85	1299.0
86	857.0
87	415.0
88	415.0
89	415.0
90	271.0
91	127.0
92	127.0
93	127.0
94	78.5
95	30.0
96	30.0
97	30.0
98	20.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07764575593247604
2	0.020478681445139098
3	0.0314295171423887
4	0.0385444668891893
5	0.04893848043234147
6	0.020354943188672998
7	0.025551949960249083
8	0.04120483940321039
9	0.041699792429074775
10	0.028212322474270177
11	0.026418117755511762
12	0.020973634471003488
13	0.02975905068009639
14	0.044360164943095866
15	0.040709886377345995
16	0.028397929858969325
17	0.026913070781376155
18	0.03248129232235053
19	0.021963540522732264
20	0.019241298880478125
21	0.015157936417096915
22	0.0226441009332958
23	0.02177793313803312
24	0.022025409650965312
25	0.1018984541998311
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1616315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.447874618678483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9318527771478	17.269565853240458
2	8.241232229095917	3.699962955643147
3	3.54059640153825	2.384365922912245
4	2.0692831834510312	1.8580403781059445
5	1.4457021505585037	1.6226470305845564
6	1.1181548198686693	1.5060119520409747
7	0.8153672605440899	1.2812283423008357
8	0.6674789253566298	1.19867865816127
9	0.5534981574508464	1.1182371516113545
>10	4.02742143015703	17.41885610450294
>50	0.3281302252188113	5.040137808277765
>100	0.19942895338017322	9.027107910817797
>500	0.02884646891549883	4.442822453515076
>1k	0.02607276998131625	11.808611764016561
>5k	0.0036058086144373537	5.765426819818309
>10k+	0.0033284387210190955	14.558298894450767
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	61512	3.8056938158712876	No Hit
GCTTATGTACTCTGCGTTGATACCA	35985	2.226360579466255	No Hit
CTTATGTACTCTGCGTTGATACCAC	23672	1.4645660035327273	No Hit
GAGTACATAAGCAGTGGTATCAACG	16467	1.0187989346136117	No Hit
TATGTACTCTGCGTTGATACCACTG	15128	0.9359561719095598	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13201	0.816734361804475	No Hit
CCCATGTACTCTGCGTTGATACCAC	12739	0.7881508245608065	No Hit
GCGTTGATACCACTGCTTATGTACT	12463	0.7710749451684851	No Hit
GTACATGATAAGCAGTGGTATCAAC	11138	0.6890983502596957	No Hit
CATAAGCAGTGGTATCAACGCAGAG	10791	0.6676297627628278	No Hit
ATCATGTACTCTGCGTTGATACCAC	10519	0.6508013598834386	No Hit
ACTCTGCGTTGATACCACTGCTTAT	10202	0.6311888462335621	No Hit
CATGTACTCTGCGTTGATACCACTG	9823	0.6077404466332368	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9049	0.5598537413808571	No Hit
CTGCTTATGTACTCTGCGTTGATAC	9023	0.5582451440467978	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	9015	0.5577501910209335	No Hit
GTATCAACGCAGAGTACATAAGCAG	8327	0.5151842307965959	No Hit
TATCAACGCAGAGTACATAAGCAGT	6956	0.43036165598908627	No Hit
GGTATCAACGCAGAGTACATAAGCA	6797	0.4205244646000315	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6107	0.377834766119228	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5883	0.36397608139502513	No Hit
GTGGTATCAACGCAGAGTACATGGG	5700	0.3526540309283772	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5485	0.33935216835827176	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5425	0.33564002066428883	No Hit
GTGGTATCAACGCAGAGTACATAAG	5007	0.3097787250628745	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4836	0.29919910413502315	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4555	0.2818138791015365	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4526	0.2800196743827781	No Hit
ACATAAGCAGTGGTATCAACGCAGA	4443	0.2748845367394351	No Hit
GAGTACATGATAAGCAGTGGTATCA	4430	0.27408023807240545	No Hit
GTACTCTGCGTTGATACCACTGCTT	4181	0.25867482514237633	No Hit
CCACTGCTTATGTACTCTGCGTTGA	3623	0.22415185158833517	No Hit
GCTTATCATGTACTCTGCGTTGATA	3613	0.2235331603060047	No Hit
GATACCACTGCTTATGTACTCTGCG	3481	0.2153664353792423	No Hit
ATACAGGGTGACAGCCCCGTACACA	3269	0.20225018019383598	No Hit
GGATACCACGTGTCCCGCCCTACTC	3227	0.19965167680804793	No Hit
GCGTTGATACCACTGCTTATCATGT	3225	0.19952793855158185	No Hit
ATACCACTGCTTATGTACTCTGCGT	3165	0.1958157908575989	No Hit
GTTGATACCACTGCTTATGTACTCT	3157	0.19532083783173454	No Hit
GATAAGCAGTGGTATCAACGCAGAG	2899	0.17935860274760798	No Hit
GTACATGGGATAAGCAGTGGTATCA	2886	0.17855430408057835	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2773	0.17156309259024385	No Hit
GCTTATCCCATGTACTCTGCGTTGA	2624	0.16234459248351962	No Hit
GTTAATGATAGTGTGTCGAAACACA	2621	0.16215898509882046	No Hit
GGTATCAACGCAGAGTACATGGGGG	2541	0.1572094548401766	No Hit
CAGTGGTATCAACGCAGAGTACATA	2500	0.15467282058262158	No Hit
TATCAACGCAGAGTACATGATAAGC	2499	0.15461095145438852	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2444	0.15120814940157085	No Hit
TACCACTGCTTATGTACTCTGCGTT	2431	0.15040385073454124	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2410	0.1491045990416472	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2365	0.14632048827116	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2307	0.1427320788336432	No Hit
TGATACCACTGCTTATGTACTCTGC	2283	0.14124721975605	No Hit
GTATCAACGCAGAGTACATGATAAG	2281	0.14112348149958395	No Hit
ATCATTAACTGAATCCATAGGTTAA	2277	0.14087600498665173	No Hit
CCCCATGTACTCTGCGTTGATACCA	2250	0.13920553852435943	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2150	0.13301862570105455	No Hit
GGTATCAACGCAGAGTACATGATAA	2111	0.13060572969996567	No Hit
ATGATAAGCAGTGGTATCAACGCAG	2062	0.1275741424165463	No Hit
GGTATCAACGCAGAGTACATGGGAA	1986	0.12287208867083459	No Hit
GTATCAACGCAGAGTACATGGGAAG	1975	0.12219152826027103	No Hit
GTATCAACGCAGAGTACATGGGGGT	1969	0.12182031349087276	No Hit
GCGTTGATACCACTGCTTCCCATGT	1929	0.1193455483615508	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1899	0.11748947451455935	No Hit
CCCATATTCAGACAGGATACCACGT	1887	0.11674704497576277	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1882	0.11643769933459754	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1868	0.11557153153933485	No Hit
CTGCTTATCATGTACTCTGCGTTGA	1836	0.1135917194358773	No Hit
CAGTGGTATCAACGCAGAGTACATG	1825	0.11291115902531375	No Hit
ATCAACGCAGAGTACATAAGCAGTG	1784	0.11037452476775876	No Hit
GGATAAGCAGTGGTATCAACGCAGA	1762	0.1090134039466317	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1744	0.1078997596384368	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1669	0.10325957502095816	No Hit
GTACATGGGGGTTAAGCGACTAAGC	1662	0.10282649112332681	No Hit
TATCAACGCAGAGTACATGGGAAGC	1646	0.10183658507159805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.186912823304864E-5	0.0	0.0	0.0	0.0
6	6.186912823304864E-5	0.0	0.0	0.0	0.0
7	6.186912823304864E-5	0.0	0.0	0.0	0.0
8	6.186912823304864E-5	0.0	0.0	0.0	0.0
9	6.186912823304864E-5	0.0	0.0	0.0	0.0
10	6.186912823304864E-5	0.0	0.0	0.0	0.0
11	6.186912823304864E-5	0.0	0.0	0.0	0.0
12	6.186912823304864E-5	0.0	0.0	6.186912823304864E-5	0.0
13	6.186912823304864E-5	0.0	0.0	6.186912823304864E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTTAG	40	2.7434694E-4	16.639046	1
CCCTATG	40	2.7545146E-4	16.630285	2
AGTTGTA	290	0.0	15.06629	11
AGATTAC	110	1.2732926E-11	14.679212	11
GTACAAA	545	0.0	14.480113	1
ATATAGC	40	0.005249725	14.262039	1
TAGAACC	40	0.0052681016	14.25453	4
TCAACTA	40	0.0052681016	14.25453	5
ATCACGG	40	0.005284361	14.2479105	6
TTGTATC	305	0.0	14.013905	13
ACTCTTC	185	0.0	13.862831	9
TACATCC	55	1.9626664E-4	13.816156	7
TGTATCA	305	0.0	13.391065	14
CCTAGAG	50	0.001496004	13.304229	2
GCGTCCG	50	0.0015016001	13.297638	13
GTACTAG	65	5.4097065E-5	13.16496	1
GTATATC	65	5.4097065E-5	13.16496	1
CTTACTC	80	1.9924173E-6	13.066652	3
GCAGGAC	95	7.356721E-8	13.004132	3
AGCAGTT	310	0.0	12.869081	8
>>END_MODULE