FastQCFastQC Report
Wed 25 May 2016
SRR1295338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616315
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA647524.006149791346365No Hit
GCTTATGTACTCTGCGTTGATACCA408842.52945743867996No Hit
CTTATGTACTCTGCGTTGATACCAC277741.7183531675446926No Hit
GTATCAACGCAGAGTACTTTTTTTT234721.4521921778861175No Hit
GAGTACATAAGCAGTGGTATCAACG182231.127441123790845No Hit
GGTATCAACGCAGAGTACTTTTTTT164041.0149011795349299No Hit
CCCATGTACTCTGCGTTGATACCAC155060.9593427023816522No Hit
TATCAACGCAGAGTACTTTTTTTTT148790.9205507589795306No Hit
GCGTTGATACCACTGCTTATGTACT145520.9003195540473238No Hit
TATGTACTCTGCGTTGATACCACTG145190.8982778728156331No Hit
CATAAGCAGTGGTATCAACGCAGAG131540.8138265127775218No Hit
ACTCTGCGTTGATACCACTGCTTAT122250.7563500926490195No Hit
GTACTTTTTTTTTTTTTTTTTTTTT122220.7561644852643203No Hit
ATCATGTACTCTGCGTTGATACCAC120980.7484927133634223No Hit
CATGTACTCTGCGTTGATACCACTG115920.7171869344774997No Hit
GTACATGATAAGCAGTGGTATCAAC111730.6912637697478524No Hit
ACGCAGAGTACATAAGCAGTGGTAT109300.6762295715872215No Hit
CTGCTTATGTACTCTGCGTTGATAC104650.6474604269588539No Hit
GTATCAACGCAGAGTACATAAGCAG87080.5387563686533875No Hit
ACGCAGAGTACTTTTTTTTTTTTTT86330.5341161840359089No Hit
TATCAACGCAGAGTACATAAGCAGT78530.4858582640141309No Hit
GGTATCAACGCAGAGTACATAAGCA74760.46253360267027155No Hit
AAGCAGTGGTATCAACGCAGAGTAC68660.4247934344481119No Hit
GTGGTATCAACGCAGAGTACATGGG62800.38853812530354537No Hit
ACATAAGCAGTGGTATCAACGCAGA54460.33693927235718285No Hit
GTACATGGGAAGCAGTGGTATCAAC54430.3367536649724837No Hit
GTACTGGTTCACTATCGGTCAGTCA53110.3285869400457213No Hit
GTGGTATCAACGCAGAGTACATAAG52800.3266689970704968No Hit
GCAGTGGTATCAACGCAGAGTACAT50960.3152850774756158No Hit
GAGTACATGATAAGCAGTGGTATCA48470.29987966454558673No Hit
GAGTACTTTTTTTTTTTTTTTTTTT45340.2805146274086425No Hit
GTACTCTGCGTTGATACCACTGCTT43330.2680789326337997No Hit
CCACTGCTTATGTACTCTGCGTTGA42000.25985033857880424No Hit
GCTTATCATGTACTCTGCGTTGATA41090.2542202479095968No Hit
GATACCACTGCTTATGTACTCTGCG40690.25174548278027487No Hit
ATACAGGGTGACAGCCCCGTACACA37920.23460773425972042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA37830.23405091210562298No Hit
GCGTTGATACCACTGCTTATCATGT37060.22928698923167823No Hit
GGATACCACGTGTCCCGCCCTACTC36840.22792586841055115No Hit
ATACCACTGCTTATGTACTCTGCGT34200.21159241855702632No Hit
ACGCAGAGTACATGATAAGCAGTGG32980.20404438491259438No Hit
GATAAGCAGTGGTATCAACGCAGAG32870.20336382450203086No Hit
GTTGATACCACTGCTTATGTACTCT32650.20200270368090378No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32520.20119840501387412No Hit
CAGTGGTATCAACGCAGAGTACATA30650.18962887803429404No Hit
GTACATGGGATAAGCAGTGGTATCA29560.18288514305689177No Hit
CTTATACACATCTCCGAGCCCACGA29130.18022477054287067No Hit
GCTTATCCCATGTACTCTGCGTTGA28320.17521337115599372No Hit
TACCACTGCTTATGTACTCTGCGTT27050.16735599187039657No Hit
CCCCATGTACTCTGCGTTGATACCA26760.16556178715163816No Hit
TATCAACGCAGAGTACATGATAAGC26700.16519057238223986No Hit
GTTAATGATAGTGTGTCGAAACACA26540.16420066633051106No Hit
TGATACCACTGCTTATGTACTCTGC26130.16166403207295607No Hit
GGTATCAACGCAGAGTACATGGGGG26050.16116907904709168No Hit
ATCATTAACTGAATCCATAGGTTAA25840.15986982735419766No Hit
GAGTACATGGGAAGCAGTGGTATCA25640.1586324447895367No Hit
GCAGAGTACATAAGCAGTGGTATCA25260.15628141791668085No Hit
ATGATAAGCAGTGGTATCAACGCAG24270.15015637422160902No Hit
GTATCAACGCAGAGTACATGATAAG23390.14471189093710077No Hit
GTGGTATCAACGCAGAGTACTTTTT23220.14366011575713894No Hit
CCCATATTCAGACAGGATACCACGT23060.14267020970541014No Hit
CAGTGGTATCAACGCAGAGTACATG22380.13846310898556285No Hit
GGTATCAACGCAGAGTACATGATAA21980.1359883438562409No Hit
ATCAACGCAGAGTACTTTTTTTTTT21950.13580273647154176No Hit
GCGTTGATACCACTGCTTCCCATGT21810.13493656867627907No Hit
GTATCAACGCAGAGTACATGGGAAG21340.13202871964932578No Hit
GGATAAGCAGTGGTATCAACGCAGA21320.1319049813928597No Hit
GGTATCAACGCAGAGTACATGGGAA21050.13023451493056737No Hit
CTGCTTATCATGTACTCTGCGTTGA20970.12973956190470298No Hit
ACTTAGATGTTTCAGTTCCCCCGGT20830.1288733941094403No Hit
GTATCAACGCAGAGTACATGGGGGT20790.12862591759650813No Hit
ATCAACGCAGAGTACATAAGCAGTG20710.1281309645706437No Hit
ACCCTGTATCGCGCGCCTTTCCAGA19930.12330517256846593No Hit
GTATTTAGCCTTGGAGGATGGTCCC19120.11829377318158898No Hit
CATGATAAGCAGTGGTATCAACGCA19000.11755134364279239No Hit
ACGCAGAGTACATGGGAAGCAGTGG18840.11656143759106363No Hit
TATCAACGCAGAGTACATGGGAAGC18740.11594274630873312No Hit
CTATCGGTCAGTCAGGAGTATTTAG18580.11495284025700436No Hit
GGTTAATGAGGCGAACCGGGGGAAC18380.1137154576923434No Hit
GCCTTGGAGGATGGTCCCCCCATAT18330.11340611205117813No Hit
GGGATAAGCAGTGGTATCAACGCAG18270.11303489728177986No Hit
TATTCAGACAGGATACCACGTGTCC18230.11278742076884765No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG18030.11155003820418669No Hit
TCTAAGTACCCCGAGGAAAAGAAAT17700.10950835697249607No Hit
AAAAAGTACTCTGCGTTGATACCAC17540.10851845092076731No Hit
GGTATCAACGCAGAGTACATGGGGA17130.10598181666321231No Hit
GCCCAGAGCCTGAATCAGTGTGTGT16880.10443508845738608No Hit
ACTCTGCGTTGATACCACTGCTTCC16780.1038163971750556No Hit
GTACACAAGCAGTGGTATCAACGCA16740.1035689206621234No Hit
AGCGTACACGGTGGATGCCCTGGCA16730.10350705153389037No Hit
GTCAGGAGTATTTAGCCTTGGAGGA16570.10251714548216158No Hit
CACACACACTGATTCAGGCTCTGGG16560.10245527635392854No Hit
TCATTAACCTATGGATTCAGTTAAT16190.10016611860930574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGTG359.923431E-519.00411819
ACTGTTC250.00604837218.9911848
TATACAC3850.016.5457823
CTAGTTG350.002176988916.27815817
CTTATAC4200.015.6405431
TTAGACA1101.0913936E-1114.6931884
TTATACA4650.014.31259252
CTATAGT400.005204871814.2804971
GTTGTAA400.005204871814.2804971
CTAGATG400.005252181614.2610344
GTTAGAC1257.2759576E-1213.6910173
CCTCTAT707.104334E-613.6004721
GTAGGAT500.001490522513.3107113
AAGTGGG500.001504846513.2938287
GGATTAG655.3444564E-513.1819961
GTTAGTT655.4117154E-513.164443
TAAGGGT655.4132885E-513.1640324
AATCGCC1750.013.02252618
GCTATCG1102.763045E-912.94933511
ATTAGAT604.0644853E-412.67686753
Produced by FastQC (version 0.11.5)