FastQCFastQC Report
Wed 25 May 2016
SRR1295337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121097
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG48150.15427268040692102No Hit
ATTCCATTCCATTCCATTCCATTCC32420.10387373413899023No Hit
GTATCAACGCAGAGTACTTTTTTTT32380.1037455740721932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG456.7557336E-414.7804585
GTCTTAC3050.010.2878111
AGGATCG856.585916E-410.0606495
GGTATCA27150.09.9812261
GCGTCAG1356.370974E-79.8605961
CCTAGAC3350.09.643543
GTGGTAT13500.09.29713251
TATTCCG1651.0636904E-79.2137935
TGGTATC13200.09.1416642
TCTATAC3800.09.0016323
CCGAGAC850.0074272018.9427993
TCCGTAC1401.0885973E-58.8196358
GTATTAA5550.08.7374771
GTCCTAA3400.08.6694531
GTGTAGG4800.08.3198771
GTGTAGA4200.08.15008451
CTAGCAC2701.3278623E-108.0940613
GTCCAGT2854.9112714E-118.00711
GTATAGC2502.0390871E-97.9870821
GTCTTGC5600.07.9802911
Produced by FastQC (version 0.11.5)