##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295337_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3121097
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.688202257091017	31.0	30.0	33.0	27.0	34.0
2	30.525391873434245	31.0	31.0	34.0	27.0	34.0
3	31.01468393965327	31.0	31.0	34.0	27.0	34.0
4	34.24052120135965	37.0	35.0	37.0	30.0	37.0
5	34.49910912733568	37.0	35.0	37.0	31.0	37.0
6	34.64848417078995	37.0	35.0	37.0	32.0	37.0
7	34.69655412824401	37.0	35.0	37.0	32.0	37.0
8	34.761571972931314	37.0	35.0	37.0	32.0	37.0
9	35.815444697809774	39.0	35.0	39.0	30.0	39.0
10	35.769147514479684	39.0	35.0	39.0	30.0	39.0
11	36.1385243713989	39.0	35.0	39.0	31.0	39.0
12	36.15342874636706	39.0	37.0	39.0	31.0	39.0
13	36.068934736728785	39.0	35.0	39.0	30.0	39.0
14	36.36847396924863	40.0	36.0	41.0	27.0	41.0
15	36.99317868044473	40.0	36.0	41.0	30.0	41.0
16	37.08781271456799	40.0	37.0	41.0	31.0	41.0
17	36.88147821102644	40.0	36.0	41.0	30.0	41.0
18	36.85009821867119	40.0	36.0	41.0	30.0	41.0
19	36.92622049234612	40.0	36.0	41.0	30.0	41.0
20	36.915875091354096	40.0	36.0	41.0	30.0	41.0
21	36.91621439513094	40.0	36.0	41.0	30.0	41.0
22	36.66823395748354	40.0	36.0	41.0	28.0	41.0
23	36.36162253207766	39.0	36.0	41.0	27.0	41.0
24	36.69171095931975	39.0	36.0	41.0	29.0	41.0
25	35.58574437128997	39.0	34.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3966289944996042
1101	2	0.6086694891486388
1101	3	0.4487092066508893
1101	4	0.38923821610867293
1101	5	0.34585614438559276
1101	6	0.2707187369693145
1101	7	0.3794088226596557
1101	8	0.4171251787511423
1101	9	0.4532914418810563
1101	10	0.6800556408102096
1101	11	0.5576052611258504
1101	12	0.5553533930273247
1101	13	0.5563122063568642
1101	14	1.1908593254590514
1101	15	0.8311646132834767
1101	16	0.6873749933457489
1101	17	1.0184431457160983
1101	18	1.0411290590616318
1101	19	1.0285341175923008
1101	20	1.0867468332773669
1101	21	1.0609441076480337
1101	22	0.8248344781309527
1101	23	-1.2308882105775396
1101	24	0.3002993488283394
1101	25	0.7805850646436525
1102	1	0.4260327875590626
1102	2	0.45691373284352466
1102	3	0.4814093203447882
1102	4	0.5193325972873168
1102	5	0.25162019224134013
1102	6	0.20765737837137976
1102	7	0.32452002523939427
1102	8	0.27856184595538735
1102	9	0.45243669165058265
1102	10	0.9621971941239806
1102	11	0.6685372862973722
1102	12	0.5542137260533551
1102	13	0.5211100245894471
1102	14	1.223003085252813
1102	15	0.8683913006188462
1102	16	0.7190234585583823
1102	17	0.9447696614274363
1102	18	0.9728500572892287
1102	19	0.9432272684377025
1102	20	0.988326271772344
1102	21	0.9039691034557222
1102	22	0.779984843724634
1102	23	0.9311308871085444
1102	24	0.9333361312509609
1102	25	0.8819375165243315
1103	1	-0.19665108660210961
1103	2	-1.5097478259970245
1103	3	-0.24431396758493307
1103	4	0.08917954715722232
1103	5	-0.16391083288742436
1103	6	0.02902089785466444
1103	7	0.07784339647444938
1103	8	0.25391346144778737
1103	9	0.9344423530695067
1103	10	0.9540492468591566
1103	11	0.6962845870730945
1103	12	0.6149923553498482
1103	13	0.5068312161056383
1103	14	-0.5321671417799507
1103	15	0.18722525189808437
1103	16	0.2536909909832019
1103	17	0.646994009460748
1103	18	0.8725127386572495
1103	19	0.7940138415062137
1103	20	0.8214295232619477
1103	21	0.8374180335085413
1103	22	0.49803403631604226
1103	23	1.2214516852302637
1103	24	1.0898263271186366
1103	25	0.929528124628483
1104	1	0.06760094492360835
1104	2	0.19464470115124755
1104	3	-0.18086243935911028
1104	4	-0.14024645447188533
1104	5	-0.019223220693767473
1104	6	0.05671569282928601
1104	7	0.13305091876750907
1104	8	0.1217348765115247
1104	9	-1.851948827742774
1104	10	-0.34653709674143585
1104	11	-0.29055738292720434
1104	12	0.148656284960353
1104	13	0.25711672907919336
1104	14	-1.3532372172676261
1104	15	-0.37905499967585854
1104	16	0.021121459968924228
1104	17	0.44268754667282906
1104	18	0.4826163389050748
1104	19	0.4738700601885171
1104	20	0.6656987793526028
1104	21	0.7047797240863929
1104	22	0.8701921645845303
1104	23	1.2589139203204809
1104	24	0.9265441846622195
1104	25	0.8270082001078833
1105	1	-0.14505184132001503
1105	2	-0.07413204646672966
1105	3	-1.2676583572172326
1105	4	-0.4667037384858759
1105	5	-0.29761888047725904
1105	6	-0.04015098167943165
1105	7	0.011492917919142087
1105	8	0.06734386492311728
1105	9	0.3956113332330631
1105	10	0.5575394131199403
1105	11	0.03829928227798973
1105	12	-0.011462343822095988
1105	13	-0.07646826266883977
1105	14	0.7280267781126923
1105	15	0.41873254501489754
1105	16	-0.0380122790121078
1105	17	0.4189840330793544
1105	18	0.3500071829588478
1105	19	0.23624106742815343
1105	20	-0.2548916554888976
1105	21	0.07356010756248565
1105	22	0.29695108223947386
1105	23	0.5036766092870764
1105	24	0.3733482297325992
1105	25	0.6370967795923761
1106	1	-1.2730471984675304
1106	2	-0.800674816788991
1106	3	-0.8605099293966347
1106	4	-1.0624690918512911
1106	5	-0.7275167638060296
1106	6	-0.5165788544444041
1106	7	-0.32884538430968746
1106	8	-0.3972100430284229
1106	9	-0.07080936658565662
1106	10	-1.5782856713251974
1106	11	-0.9059077159092084
1106	12	-0.6341398090333072
1106	13	-0.6553524130659554
1106	14	0.09338327354156917
1106	15	-0.7204520866217123
1106	16	-1.0309128880604561
1106	17	-0.3292040806966199
1106	18	-0.3274864592024258
1106	19	-0.1941930914460812
1106	20	0.005955273838019082
1106	21	-0.010846428947814957
1106	22	-0.09240113550509221
1106	23	-0.36184160347554695
1106	24	-0.512163462169795
1106	25	-1.1028098416891083
1107	1	0.02448661219277426
1107	2	0.21292900182676178
1107	3	0.05504297434242389
1107	4	0.2159716400500642
1107	5	0.001122854332400891
1107	6	0.07562480506235403
1107	7	-0.039028359647794275
1107	8	-0.06705888948824423
1107	9	0.5091031234700267
1107	10	0.04955840186493532
1107	11	0.1325171050931786
1107	12	0.07063346065643117
1107	13	0.16795705391892568
1107	14	-0.9286135442786332
1107	15	-0.3152014899630018
1107	16	-0.09125497238822078
1107	17	0.1729836679111827
1107	18	0.3834522838365544
1107	19	0.29526593798907896
1107	20	0.4221446135357141
1107	21	0.46469056389228314
1107	22	-0.07151808061248488
1107	23	0.6401074816477603
1107	24	0.5404220589121493
1107	25	0.1856945338081104
1108	1	-0.2323050393722177
1108	2	0.09098107924764776
1108	3	-0.30819384502784075
1108	4	-0.4140067275099426
1108	5	-0.4336465630050128
1108	6	-0.4115033232642844
1108	7	-0.33583387056317093
1108	8	-0.3653271545392016
1108	9	0.1182374155603938
1108	10	0.09320008294942994
1108	11	-0.15947045796345094
1108	12	-0.19205388561989167
1108	13	-0.36121938609721127
1108	14	-0.18661709117817793
1108	15	-0.3361261457017477
1108	16	-0.32238824782228903
1108	17	-0.012564654036061995
1108	18	-0.05535834227327996
1108	19	-0.05066766478096696
1108	20	0.12760572187343655
1108	21	0.09923784410874248
1108	22	-0.050473409281970305
1108	23	-0.30248369259026475
1108	24	-0.08776439494054955
1108	25	-1.0054873405322624
1109	1	-0.7651366428074411
1109	2	-0.726046428708301
1109	3	-0.66806694620211
1109	4	-0.5638218483650235
1109	5	-0.8458666187263546
1109	6	-1.0306332617964316
1109	7	-0.7849966632884886
1109	8	-1.109563601689132
1109	9	-0.865269105739344
1109	10	-1.0385422593286506
1109	11	-1.1231192153298792
1109	12	-1.0535572126518815
1109	13	-1.1141017569889513
1109	14	-0.4947741651337978
1109	15	-1.0883914601548241
1109	16	-1.2126298787218772
1109	17	-1.2999643377797696
1109	18	-2.047580736230323
1109	19	-2.071083831104822
1109	20	-1.828008223522815
1109	21	-1.673372300663715
1109	22	-1.7170681454774623
1109	23	-1.2272240097184337
1109	24	-2.049868331499063
1109	25	-1.3992392973184948
1110	1	0.02557883826818852
1110	2	0.12590822141671865
1110	3	0.05495518249420783
1110	4	0.31535102708487983
1110	5	0.18745974495959672
1110	6	0.15236306471192052
1110	7	0.06385603262118877
1110	8	0.03198183395598875
1110	9	0.4210870782878047
1110	10	0.3035111568921067
1110	11	0.1124280416461545
1110	12	0.20372052213103586
1110	13	0.1701348373752012
1110	14	-2.5152109762552968
1110	15	-0.6924377950030305
1110	16	-0.13599611564193026
1110	17	-0.23089850504656795
1110	18	-2.594378664150206
1110	19	-0.5083549888594305
1110	20	-1.8946475913059757
1110	21	-0.2940507306195457
1110	22	-1.683916009800953
1110	23	-0.39274231828382256
1110	24	0.12930082856225056
1110	25	0.021133166434729844
1111	1	-0.18703198637254914
1111	2	-1.3253845594013
1111	3	-0.5077889403023015
1111	4	-0.020459213234367724
1111	5	-0.04924277591811688
1111	6	0.07986860578333221
1111	7	0.022524113586307237
1111	8	-0.08528206613954836
1111	9	-1.761732059210182
1111	10	-0.4258436365637621
1111	11	-0.2384146575716528
1111	12	-0.16553231318772532
1111	13	-0.019034030734324858
1111	14	0.6706125970002716
1111	15	0.13997540111970608
1111	16	0.18154691882863716
1111	17	-0.1294385127221389
1111	18	-0.0025183676226205876
1111	19	-0.18696649543864652
1111	20	0.19498935594027955
1111	21	-0.6713882120754207
1111	22	-0.19223965015260802
1111	23	-0.1651697839343953
1111	24	-0.5546485520262081
1111	25	-2.0405288829324917
1112	1	0.14459312596708784
1112	2	0.3121109908122328
1112	3	-0.035535665352650625
1112	4	0.2672045808427441
1112	5	0.13729629851884084
1112	6	0.14597452169755343
1112	7	0.06403902777951487
1112	8	0.01565008724399064
1112	9	-0.36320444928693973
1112	10	0.3937943940418194
1112	11	0.1210118632675119
1112	12	0.11259807198579352
1112	13	0.10977963662405443
1112	14	0.5331025092644595
1112	15	0.19077617630512833
1112	16	0.1260339735378082
1112	17	-3.8677147651576362
1112	18	-1.0718870999469488
1112	19	-1.1198020987998731
1112	20	-0.08037304771601583
1112	21	-0.4843741706926892
1112	22	-1.7020192277667618
1112	23	-1.406436591080741
1112	24	-0.28234022087039534
1112	25	0.022054554058343
1113	1	-0.41168268562787347
1113	2	-0.15894342917835758
1113	3	-0.24845845274352385
1113	4	-0.6704921100454371
1113	5	-0.2509658514454358
1113	6	-0.27192151747507864
1113	7	-0.4549705039959733
1113	8	-0.42568680090140987
1113	9	0.018784502914776624
1113	10	0.1398797443824904
1113	11	-0.16641908799322636
1113	12	-0.21285891304566462
1113	13	-0.6286611252209582
1113	14	-1.7600143987460655
1113	15	-1.9518188365026532
1113	16	-0.8010801812359247
1113	17	-0.577166383119895
1113	18	-0.8695137401267772
1113	19	-0.4087386175236247
1113	20	0.007237386702925619
1113	21	-1.7946709277979522
1113	22	-2.163662108558576
1113	23	-0.9271747373508035
1113	24	-2.5625413117097793
1113	25	-0.8100433078267528
1114	1	-0.0037658901598049965
1114	2	0.1199083913282557
1114	3	-0.22238826800966294
1114	4	0.19783696823667896
1114	5	-0.022682102324999676
1114	6	0.04949298368142507
1114	7	0.023934366499872795
1114	8	-0.06477372484935984
1114	9	0.30461922920105877
1114	10	0.006704846538880815
1114	11	-0.014095064196197882
1114	12	-0.037815717828735274
1114	13	-0.03438380349967929
1114	14	0.8045954533022979
1114	15	0.2586475066171303
1114	16	0.18620546002312466
1114	17	-0.6133788059046452
1114	18	-0.2683449434784819
1114	19	-0.7050251517685027
1114	20	-1.8223368435949823
1114	21	-0.9288673058635268
1114	22	-1.6253828907913856
1114	23	-3.090950949900858
1114	24	-2.1316883221385936
1114	25	-1.7186277494661297
1115	1	0.15343164039262192
1115	2	0.22221915082089794
1115	3	0.13777195338585457
1115	4	0.3612676460369926
1115	5	0.2642617594503207
1115	6	0.11143229401282184
1115	7	0.20763086976413092
1115	8	0.21163336022098633
1115	9	0.4325825041917284
1115	10	0.5486471525376189
1115	11	0.25540745307409196
1115	12	0.19376001263035647
1115	13	0.0889458128053846
1115	14	0.8365852622956638
1115	15	0.27090901916565713
1115	16	0.2939522459563122
1115	17	0.2709436280086379
1115	18	0.5618637570704053
1115	19	-0.23140055697724193
1115	20	0.4119353649505797
1115	21	-0.6685158426653715
1115	22	0.6125151095289425
1115	23	0.4958915249929703
1115	24	0.6273194506291659
1115	25	0.7042810578675827
1116	1	0.26307749793951274
1116	2	0.31622312872125136
1116	3	0.15820379560183184
1116	4	0.580903984418562
1116	5	0.426338042144792
1116	6	0.3168350159230968
1116	7	0.21329811415731825
1116	8	0.15454802397047018
1116	9	0.38342133084415764
1116	10	0.32954507364080854
1116	11	0.2504749298259341
1116	12	0.22139078794356237
1116	13	-0.21948939963651526
1116	14	0.7066778729906957
1116	15	0.38683433154984215
1116	16	0.2921426782822394
1116	17	0.5461858353617686
1116	18	0.4747977576131248
1116	19	0.654428008165155
1116	20	0.440628712852039
1116	21	-0.11198388199061782
1116	22	-0.02154367342291863
1116	23	-0.9656190929378141
1116	24	-0.4050452921633507
1116	25	-1.2258077189362595
1201	1	0.4123614518661114
1201	2	0.6478992906582839
1201	3	0.5284634054853257
1201	4	0.40902757547728896
1201	5	0.39015089073513565
1201	6	0.29226827353841855
1201	7	0.3374231664372118
1201	8	0.3675724809596943
1201	9	0.5955663391950949
1201	10	0.8998933862350356
1201	11	0.5673862335780555
1201	12	0.6232583843178361
1201	13	0.5401754566095462
1201	14	1.1198377345255253
1201	15	0.7488690712521233
1201	16	0.5397620387856321
1201	17	0.7031897103061056
1201	18	0.5936542735731507
1201	19	0.9205100966035076
1201	20	0.8995874173508298
1201	21	0.880181865975814
1201	22	0.8085853652086499
1201	23	0.42212785359312477
1201	24	0.7932462747009055
1201	25	0.9751050749660095
1202	1	0.3860423876550634
1202	2	0.7342277244708342
1202	3	0.6038746206280408
1202	4	0.5642676264695439
1202	5	0.38305217883011267
1202	6	0.27368476133428743
1202	7	0.3618187595712783
1202	8	0.4306342910495857
1202	9	0.930754542055972
1202	10	0.1856586304190131
1202	11	0.5382208150654364
1202	12	0.4835606665815888
1202	13	0.4486379973481647
1202	14	1.1308512393646097
1202	15	0.8798962476010885
1202	16	0.8055574751590555
1202	17	-1.350950144510115
1202	18	0.21965542450256237
1202	19	0.6364267634578624
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2210	25	0.8862731975037619
2211	1	0.2153298663705563
2211	2	-0.46224107817361215
2211	3	0.026974200772350798
2211	4	0.36776278955384356
2211	5	0.3037596516890275
2211	6	0.29468854956805046
2211	7	0.2859691176064203
2211	8	0.32753375160364584
2211	9	0.640351730093002
2211	10	0.7902302266666936
2211	11	0.4151427789009716
2211	12	0.29201719258360015
2211	13	0.30708026482282236
2211	14	-0.7406219315283238
2211	15	0.09483293671662096
2211	16	0.25752683474258475
2211	17	0.36697120202691735
2211	18	0.4003468189825057
2211	19	0.6771327933336622
2211	20	0.6494647769627022
2211	21	0.7084773343787418
2211	22	0.07691852102600905
2211	23	0.6918572111859476
2211	24	0.08930677060337189
2211	25	0.41272229339865163
2212	1	-0.1412197770142214
2212	2	-1.1779482400558834
2212	3	-0.47658345205316266
2212	4	0.0014813526972687896
2212	5	-0.0026661462424826254
2212	6	0.051378716084187204
2212	7	-0.013433847960499179
2212	8	-0.02313643418531086
2212	9	-2.308071218845356
2212	10	-0.16303944817713756
2212	11	-0.05332454814885779
2212	12	-0.17915909557343923
2212	13	-0.1221582924579181
2212	14	0.4412695475947359
2212	15	0.1314540580232162
2212	16	0.20565910478422467
2212	17	0.16625370300623388
2212	18	-0.03222277868692203
2212	19	-0.774315898158001
2212	20	0.10276380300896903
2212	21	0.12512528351540908
2212	22	-1.9291674149128255
2212	23	-0.5274952416037593
2212	24	0.03860236063242439
2212	25	-0.6816055583993119
2213	1	0.022221646187837507
2213	2	0.27960872828850114
2213	3	-0.05879973292669405
2213	4	0.2642695108376003
2213	5	0.16707643444772202
2213	6	0.15381438825301785
2213	7	0.10067239246285453
2213	8	0.06746631708090689
2213	9	0.46232036749299965
2213	10	0.4880112059240602
2213	11	-0.07681611394753673
2213	12	-0.09257290382738859
2213	13	-0.03223764728274148
2213	14	-1.7643421698120747
2213	15	-0.5455271260922601
2213	16	-0.35010216828250407
2213	17	-2.3107736972464394
2213	18	-1.0285105272711377
2213	19	-0.7783260460608901
2213	20	0.05488683197095412
2213	21	-0.4945599638181619
2213	22	-0.03924528494206925
2213	23	0.5564154309943916
2213	24	0.4008159553757693
2213	25	-1.0747530693157472
2214	1	0.14532315506590976
2214	2	0.2012537600180444
2214	3	0.11698545564758689
2214	4	0.5176770339944738
2214	5	0.444790695374941
2214	6	0.3378181332417185
2214	7	0.2395931354061247
2214	8	0.10480688089251089
2214	9	0.5706765953900046
2214	10	0.44238105239995207
2214	11	-0.15689171755174414
2214	12	-0.14207519450780381
2214	13	0.062168283496546906
2214	14	0.8473431558140163
2214	15	0.4631087557299054
2214	16	0.3893899241277978
2214	17	0.2924455455936865
2214	18	0.3124075710301497
2214	19	-0.9685744862641457
2214	20	0.04248224543084689
2214	21	0.3701578658724429
2214	22	0.5919513231777884
2214	23	1.045789220640735
2214	24	0.8331288152448195
2214	25	0.7546467903542862
2215	1	-0.14149302888025161
2215	2	0.2070150924771248
2215	3	0.11209896289442511
2215	4	0.4220844593654931
2215	5	0.3903666721698471
2215	6	0.31767683012684955
2215	7	0.19011144412832692
2215	8	0.13880849574739784
2215	9	0.2942803208643596
2215	10	0.6169039947215111
2215	11	0.21975551373118662
2215	12	0.22171067344279294
2215	13	-0.007671871899233906
2215	14	0.8855727274535141
2215	15	0.37903518315629725
2215	16	0.2966968560917209
2215	17	0.568281908679559
2215	18	0.41183820239040614
2215	19	-0.20149916490736075
2215	20	-1.4820813215459836
2215	21	-1.8062165756530106
2215	22	-0.19651659756737416
2215	23	0.49594414609816084
2215	24	0.637211831769747
2215	25	-0.059440049965587605
2216	1	-0.11828272513709592
2216	2	-0.2918803068552691
2216	3	-0.26431469858822965
2216	4	0.22803340076664114
2216	5	0.293500322944098
2216	6	0.016892075129241846
2216	7	0.030457109446317077
2216	8	-0.10629286490048173
2216	9	0.13681634192433023
2216	10	-1.637724544215203
2216	11	-0.49164149113116906
2216	12	-0.15590408841705994
2216	13	-0.0856072893550035
2216	14	-0.5483803229981135
2216	15	-0.5582809364989174
2216	16	-0.1313803991459963
2216	17	0.3213592419467304
2216	18	0.015632677644738635
2216	19	-1.3660014000460805
2216	20	-0.9998226301816118
2216	21	-0.7957823198482998
2216	22	-0.8017163140379893
2216	23	-4.6545452249674995
2216	24	-2.1546347981304947
2216	25	-1.2436535903702435
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	41.0
5	49.0
6	78.0
7	273.0
8	145.0
9	317.0
10	937.0
11	1918.0
12	3439.0
13	5492.0
14	8034.0
15	10209.0
16	12101.0
17	13399.0
18	14119.0
19	14713.0
20	14696.0
21	15333.0
22	15875.0
23	16895.0
24	18517.0
25	20859.0
26	23872.0
27	28104.0
28	33573.0
29	42350.0
30	53220.0
31	67368.0
32	91604.0
33	126870.0
34	179168.0
35	257213.0
36	381632.0
37	596516.0
38	1052157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59613462499828	21.628611163040365	14.072076237775203	22.703177974186154
2	15.37080804957033	21.708907308803475	40.91003920952822	22.010245432097975
3	17.63981907877868	26.8884421698239	30.80965195228767	24.662086799109755
4	12.66269461972266	17.970916103009102	39.37589629085436	29.99049298641388
5	13.336842449448532	37.49537585262225	35.663187683643564	13.50459401428565
6	29.842565836847484	39.13386374335479	18.197334447692164	12.826235972105565
7	25.146762758697605	33.01352864525747	24.756741408473815	17.082967187571104
8	22.804035646199548	37.42290054218018	22.144804031800042	17.62825977982023
9	23.89970791357729	17.671276651323556	23.063352913668638	35.365662521430515
10	14.601257273066699	30.233587426628354	34.437872667050186	20.72728263325476
11	30.9328795286328	26.467293852965355	24.391810363476413	18.208016254925433
12	20.28729600461468	27.95314815853356	32.33017409208534	19.42938174476642
13	25.47708868617572	24.997988928369637	27.56023840573138	21.964683979723272
14	20.3935009267952	24.04725036868275	28.78196706231083	26.77728164221122
15	22.139770784861685	31.374435047217087	24.906673213778035	21.579120954143193
16	21.513106616892973	31.866904510399145	26.17852509537679	20.44146377733109
17	19.64521041755334	30.667454632160272	29.191333319659314	20.496001630627074
18	19.73035130991267	30.620458636090923	29.84897160702107	19.80021844697533
19	20.466766908811696	29.30297739680635	30.058254530445026	20.172001163936926
20	21.77525001986828	29.01890434769233	29.231494940638708	19.974350691800684
21	22.261968049738147	29.526898610968246	28.020808398728093	20.190324940565514
22	20.973112798956315	29.106410197658626	29.836233580656312	20.08424342272874
23	21.34275428131195	29.410829689061284	28.93368268779031	20.312733341836456
24	20.935010414997596	28.540041659990383	29.37333760615286	21.151610318859156
25	21.733440634414972	28.864546925031764	28.663116835546127	20.73889560500714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1999.0
1	1999.0
2	2290.5
3	2582.0
4	2582.0
5	2582.0
6	3953.0
7	5324.0
8	5324.0
9	5324.0
10	9220.0
11	13116.0
12	13116.0
13	13116.0
14	22883.0
15	32650.0
16	32650.0
17	32650.0
18	51791.5
19	70933.0
20	70933.0
21	70933.0
22	101138.0
23	131343.0
24	131343.0
25	131343.0
26	169261.5
27	207180.0
28	207180.0
29	207180.0
30	246734.5
31	286289.0
32	286289.0
33	286289.0
34	318996.5
35	351704.0
36	351704.0
37	351704.0
38	371750.5
39	391797.0
40	391797.0
41	391797.0
42	386360.5
43	380924.0
44	380924.0
45	380924.0
46	361029.5
47	341135.0
48	341135.0
49	341135.0
50	320673.0
51	300211.0
52	300211.0
53	300211.0
54	267826.0
55	235441.0
56	235441.0
57	235441.0
58	201450.5
59	167460.0
60	167460.0
61	167460.0
62	136480.5
63	105501.0
64	105501.0
65	105501.0
66	80317.5
67	55134.0
68	55134.0
69	55134.0
70	40035.0
71	24936.0
72	24936.0
73	24936.0
74	17527.0
75	10118.0
76	10118.0
77	10118.0
78	6861.0
79	3604.0
80	3604.0
81	3604.0
82	2435.0
83	1266.0
84	1266.0
85	1266.0
86	798.5
87	331.0
88	331.0
89	331.0
90	210.5
91	90.0
92	90.0
93	90.0
94	58.5
95	27.0
96	27.0
97	27.0
98	14.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08131756238271351
2	0.021851291388893072
3	0.03412261778470839
4	0.04149182162553743
5	0.051360146768908495
6	0.019800730320140642
7	0.024190212607938813
8	0.03527605838588163
9	0.03620521887016007
10	0.02729809422776671
11	0.026016493559796447
12	0.01992889038693767
13	0.02787481452835333
14	0.038640260139303585
15	0.03671785913734818
16	0.026945654044074886
17	0.026433013776886782
18	0.028483574845639206
19	0.020922130904614627
20	0.0174618091010949
21	0.013905367247477408
22	0.019736650286742128
23	0.021627011271998276
24	0.019127889969456253
25	0.10861565661048021
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3121097.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.2584404674655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.94020604815312	74.88281290891737
2	7.856740566921521	13.082799335187131
3	1.2054413008408011	3.010894883492341
4	0.35070948335007973	1.167980985635128
5	0.17935360015631552	0.7466350520620104
6	0.10184732119284529	0.5087789476983209
7	0.07010982608605226	0.4086064346958959
8	0.049034576002012796	0.32660338615287965
9	0.033587438853851945	0.2516794000441263
>10	0.1932783909280211	2.835096954927102
>50	0.011529806181033475	0.6592924870093755
>100	0.007275712732558538	1.09886447797616
>500	5.007422536698083E-4	0.2835630699215396
>1k	3.851863482552505E-4	0.7363916762805613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4815	0.15427268040692102	No Hit
ATTCCATTCCATTCCATTCCATTCC	3242	0.10387373413899023	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3238	0.1037455740721932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.20400166992567E-5	0.0
5	0.0	0.0	0.0	3.20400166992567E-5	0.0
6	0.0	0.0	0.0	3.20400166992567E-5	0.0
7	0.0	0.0	0.0	9.612005009777011E-5	0.0
8	0.0	0.0	0.0	9.612005009777011E-5	0.0
9	0.0	0.0	0.0	9.612005009777011E-5	0.0
10	0.0	0.0	0.0	9.612005009777011E-5	0.0
11	0.0	0.0	0.0	9.612005009777011E-5	0.0
12	0.0	0.0	0.0	1.281600667970268E-4	0.0
13	0.0	0.0	0.0	1.6020008349628353E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACCG	45	6.7557336E-4	14.780458	5
GTCTTAC	305	0.0	10.287811	1
AGGATCG	85	6.585916E-4	10.060649	5
GGTATCA	2715	0.0	9.981226	1
GCGTCAG	135	6.370974E-7	9.860596	1
CCTAGAC	335	0.0	9.64354	3
GTGGTAT	1350	0.0	9.2971325	1
TATTCCG	165	1.0636904E-7	9.213793	5
TGGTATC	1320	0.0	9.141664	2
TCTATAC	380	0.0	9.001632	3
CCGAGAC	85	0.007427201	8.942799	3
TCCGTAC	140	1.0885973E-5	8.819635	8
GTATTAA	555	0.0	8.737477	1
GTCCTAA	340	0.0	8.669453	1
GTGTAGG	480	0.0	8.319877	1
GTGTAGA	420	0.0	8.1500845	1
CTAGCAC	270	1.3278623E-10	8.094061	3
GTCCAGT	285	4.9112714E-11	8.0071	1
GTATAGC	250	2.0390871E-9	7.987082	1
GTCTTGC	560	0.0	7.980291	1
>>END_MODULE