FastQCFastQC Report
Wed 25 May 2016
SRR1295337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121097
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113350.36317358928607474No Hit
GGTATCAACGCAGAGTACTTTTTTT97270.31165324243366993No Hit
TATCAACGCAGAGTACTTTTTTTTT66510.21309815106675634No Hit
GTGGTATCAACGCAGAGTACATGGG53790.1723432498253018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45010.14421211516335442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG350.00214071616.319841
TGGACCG456.7252875E-414.7892675
GGACCGC751.4854595E-512.6618936
CGTCCAA658.053907E-411.68771410
TTAGACG752.0601673E-411.4088644
GGTATCA35350.011.0414761
GTGGTAT15700.010.7326341
AGCGTCA1951.8189894E-1210.7262823
TATTCCG909.446486E-510.5637625
TGGTATC15500.010.4274562
CGTAGAC750.00263182710.1412123
ATCCGTA750.002657499210.12935311
CAGCGTC2105.456968E-129.9601182
GTCTAAG2550.09.3332411
GACTGTC2401.0913936E-119.100597
TCCGTAC1151.1018758E-49.0940213
CTAGGAC3150.09.0546533
CTGTGCG850.00746227858.9376639
TGTCCGT850.00746227858.93766310
ATTATAC4450.08.7596153
Produced by FastQC (version 0.11.5)