FastQCFastQC Report
Wed 25 May 2016
SRR1295336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054164
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59710.14728067241483078No Hit
GTATCAACGCAGAGTACTTTTTTTT52440.1293484920688951No Hit
GGTATCAACGCAGAGTACTTTTTTT42670.10524981229175731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAT500.001501544313.2985067
GTCGTAG1053.443054E-610.8642351
GGTATCA33850.09.99766351
TACACGG1054.0981206E-59.9547025
TGGTATC16400.09.5596842
GTGGTAT17050.09.4783291
GCGTCAG1951.6661943E-99.2624561
ACACCGT1502.708579E-68.865676
TAGACTG4100.08.80693155
CGTCAGA2054.132744E-98.8064972
GTTCTAG4000.08.793241
TAGGGTG4400.08.6383785
GAACCGT1000.00290944648.5490396
TCTAGAC3950.08.4195813
CTAGACT3750.08.361954
TACTGCG1252.7484586E-48.359067
TAGACAG7050.08.3565595
TTAGGAC3300.08.3503293
TAGGACT4600.08.2627964
TATACAG7450.08.1629775
Produced by FastQC (version 0.11.5)