##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4054164 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.378687196669894 31.0 30.0 33.0 27.0 34.0 2 30.207974813056403 31.0 30.0 34.0 26.0 34.0 3 30.58481427983673 31.0 30.0 34.0 27.0 34.0 4 33.97015192281319 35.0 35.0 37.0 28.0 37.0 5 34.27650756111494 35.0 35.0 37.0 30.0 37.0 6 34.64788498935909 37.0 35.0 37.0 32.0 37.0 7 34.61977734497174 37.0 35.0 37.0 31.0 37.0 8 34.60147714793975 37.0 35.0 37.0 31.0 37.0 9 35.721686887851604 39.0 35.0 39.0 30.0 39.0 10 35.63100308719628 39.0 35.0 39.0 29.0 39.0 11 36.038389912198916 39.0 35.0 39.0 30.0 39.0 12 36.04154543328785 39.0 35.0 39.0 30.0 39.0 13 35.97143504801483 39.0 35.0 39.0 30.0 39.0 14 36.23983489567763 39.0 36.0 41.0 27.0 41.0 15 36.87105726359368 40.0 36.0 41.0 30.0 41.0 16 36.96739821082719 40.0 36.0 41.0 30.0 41.0 17 36.75977834147805 40.0 36.0 41.0 30.0 41.0 18 36.726089275125524 39.0 36.0 41.0 29.0 41.0 19 36.791351311885755 40.0 36.0 41.0 29.0 41.0 20 36.78268368028526 40.0 36.0 41.0 29.0 41.0 21 36.77995734755673 40.0 36.0 41.0 29.0 41.0 22 36.53604639575508 39.0 36.0 41.0 27.0 41.0 23 36.226170672918016 39.0 35.0 41.0 27.0 41.0 24 36.55172410390897 39.0 36.0 41.0 28.0 41.0 25 35.420463750356426 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3187721758697215 1101 2 0.5516216185551421 1101 3 0.391344969249694 1101 4 0.26030495383961494 1101 5 0.23570157127138458 1101 6 0.22804846969570747 1101 7 0.23988057412390873 1101 8 0.2469315606361917 1101 9 0.2698600817198553 1101 10 0.616963253558815 1101 11 0.5383456741658676 1101 12 0.5620038231928177 1101 13 0.5344456320971176 1101 14 1.0798559854588134 1101 15 0.8087101105438848 1101 16 0.5814995383181696 1101 17 0.9686643250024289 1101 18 0.9482179382531442 1101 19 0.8956957876148977 1101 20 0.976088357900565 1101 21 0.9687934225500356 1101 22 0.7541300655270149 1101 23 -1.5366224722208983 1101 24 0.19382999190928984 1101 25 0.7127256144567795 1102 1 0.19218212848398863 1102 2 0.22117274263746722 1102 3 0.2008620600041624 1102 4 0.2814219653494163 1102 5 0.11641129420497975 1102 6 0.21609782687975354 1102 7 0.19690556518472135 1102 8 0.2630438367941821 1102 9 0.5036443204073606 1102 10 0.8814473901302264 1102 11 0.6000621264531674 1102 12 0.4652631626273447 1102 13 0.5369275040751091 1102 14 1.06496958558251 1102 15 0.7107305273192708 1102 16 0.5739779795820894 1102 17 0.9517595216806001 1102 18 0.9156373486857419 1102 19 0.957943436850961 1102 20 0.9400640078839757 1102 21 0.9650652993922364 1102 22 0.810125439700343 1102 23 0.9106211986753721 1102 24 0.8595604601937126 1102 25 0.9695661845029449 1103 1 -0.3683890149337685 1103 2 -1.5253223915745906 1103 3 -0.4865834720093858 1103 4 -0.10278797318502342 1103 5 -0.2268162414614423 1103 6 0.021500519602838608 1103 7 0.07472840074314036 1103 8 0.25104753490610676 1103 9 0.8486971909677905 1103 10 0.9452847467152949 1103 11 0.6426882382899564 1103 12 0.4804400104110229 1103 13 0.32891849063410916 1103 14 -0.8536353101748375 1103 15 -0.032505037886814137 1103 16 0.07882502250620149 1103 17 0.5681649738511183 1103 18 0.7117247293028797 1103 19 0.6986605539069473 1103 20 0.6925503661484598 1103 21 0.8482606320809438 1103 22 0.44965486343950545 1103 23 1.1493607871331974 1103 24 0.9482922170230594 1103 25 0.8811107682391679 1104 1 -0.00963980622463012 1104 2 -0.0540641084289426 1104 3 -0.30075698685081065 1104 4 -0.3252256426954929 1104 5 -0.17480252962489118 1104 6 0.00979493207299953 1104 7 0.03865259362645901 1104 8 0.09943742496749763 1104 9 -1.912757593655904 1104 10 -0.3898957256780875 1104 11 -0.3638775883984735 1104 12 0.18321472627226143 1104 13 0.19759132888555087 1104 14 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0.23853655472289148 2214 19 -0.8788688011677408 2214 20 0.0355134373022139 2214 21 0.37955535128838847 2214 22 0.5663342383630479 2214 23 1.0604446091976243 2214 24 0.7650514617181727 2214 25 0.8174488927601118 2215 1 -0.2072745254304067 2215 2 0.21826197891820343 2215 3 0.13311853500611903 2215 4 0.3646255025710303 2215 5 0.31990833453641443 2215 6 0.09418094714655467 2215 7 0.004937644650610196 2215 8 -0.013120521563628529 2215 9 0.15520467586365783 2215 10 0.43007387763067584 2215 11 0.17314130261001992 2215 12 -0.026470067503545636 2215 13 -0.15029030173087676 2215 14 0.732141690468346 2215 15 0.29144203206595165 2215 16 0.22658304834495624 2215 17 0.3326963059515293 2215 18 0.15017204945151263 2215 19 -0.7042588780137891 2215 20 -1.8533891396849569 2215 21 -2.066458237321825 2215 22 -0.3881406322833669 2215 23 0.28073837997965967 2215 24 0.43873846749823997 2215 25 -0.17142385053936238 2216 1 0.08624999816647261 2216 2 -0.1772702003365474 2216 3 -0.02983553741321643 2216 4 0.3116016934292176 2216 5 0.31489108413708067 2216 6 -0.08670829029012594 2216 7 -0.026960399262847545 2216 8 -0.10493368483687959 2216 9 0.15008626086819277 2216 10 -1.7088911765058583 2216 11 -0.604638533687968 2216 12 -0.34272279758943824 2216 13 -0.14199465629481267 2216 14 -0.6475160722707756 2216 15 -0.637706609815794 2216 16 -0.1953729966036235 2216 17 0.15842410744261315 2216 18 0.05209094681131177 2216 19 -1.5154307557925222 2216 20 -1.218413264268463 2216 21 -1.0928123043253422 2216 22 -0.8352073555869239 2216 23 -4.686355856189227 2216 24 -2.22302300017828 2216 25 -1.3957564591886964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 13.0 5 39.0 6 57.0 7 264.0 8 194.0 9 495.0 10 1298.0 11 2641.0 12 4494.0 13 7364.0 14 10496.0 15 13350.0 16 15792.0 17 17693.0 18 18567.0 19 19367.0 20 19790.0 21 20585.0 22 21539.0 23 23310.0 24 25946.0 25 28923.0 26 33523.0 27 39554.0 28 46691.0 29 58686.0 30 73375.0 31 93385.0 32 127150.0 33 174395.0 34 244742.0 35 349838.0 36 513699.0 37 799745.0 38 1247154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54432066464396 21.416505657476613 14.06619236531325 22.97298131256617 2 15.85679080692525 21.41819741900563 40.289495309985526 22.435516464083598 3 18.055514775820708 26.43866586691781 30.439342906468614 25.06647645079287 4 12.6078087420543 18.23825469820708 38.85171305879616 30.302223500942464 5 13.49832897738055 37.59844079017666 35.53657926875764 13.366650963685148 6 30.055636429390976 38.905758504889064 18.121248586763645 12.917356478956316 7 25.523242015868842 32.97257318283213 24.057291047342556 17.44689375395648 8 23.29623388861185 36.84585056113044 21.73531342891003 18.12260212134768 9 24.18722020810854 17.480332679544876 22.61018276549803 35.722264346848554 10 14.81029643487213 30.078381054141424 34.260866733458286 20.850455777528165 11 31.255424412979217 25.922588106775642 24.37358052456263 18.448406955682515 12 20.716344712831816 27.603817314583086 31.78554556589333 19.894292406691772 13 25.939775610439668 24.483631641118812 27.307448632080266 22.269144116361254 14 20.712032004477035 23.886496920681928 28.45692594264096 26.944545132200076 15 22.27804331246149 31.233170706190666 24.755473275581082 21.73331270576676 16 21.578639500995596 31.383662554760246 26.168002721698663 20.869695222545495 17 20.035994518175503 30.38963634188131 28.701569918549758 20.87279922139342 18 20.164785617094875 30.10894009466723 29.503040231721293 20.223234056516606 19 20.871443528166104 29.190945520964597 29.723386346345322 20.214224604523984 20 21.92157514781689 28.79269224089614 29.133199002186664 20.15253360910031 21 22.33197593186088 29.08648722786189 28.107732353451954 20.47380448682528 22 21.46203478793746 28.696890617776262 29.362517188959398 20.47855740532689 23 21.71394245001875 28.89609524561369 28.768872486135503 20.621089818232054 24 21.33791242814224 28.38220557375933 29.100030738913148 21.179851259185288 25 21.873455154604297 28.615561719984257 28.54321171783598 20.967771407575462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2495.0 1 2495.0 2 2869.5 3 3244.0 4 3244.0 5 3244.0 6 4999.5 7 6755.0 8 6755.0 9 6755.0 10 11557.0 11 16359.0 12 16359.0 13 16359.0 14 28296.0 15 40233.0 16 40233.0 17 40233.0 18 64347.5 19 88462.0 20 88462.0 21 88462.0 22 126148.5 23 163835.0 24 163835.0 25 163835.0 26 212563.0 27 261291.0 28 261291.0 29 261291.0 30 311448.5 31 361606.0 32 361606.0 33 361606.0 34 401640.0 35 441674.0 36 441674.0 37 441674.0 38 464814.0 39 487954.0 40 487954.0 41 487954.0 42 485779.0 43 483604.0 44 483604.0 45 483604.0 46 464049.5 47 444495.0 48 444495.0 49 444495.0 50 421003.5 51 397512.0 52 397512.0 53 397512.0 54 359689.0 55 321866.0 56 321866.0 57 321866.0 58 277610.0 59 233354.0 60 233354.0 61 233354.0 62 192175.5 63 150997.0 64 150997.0 65 150997.0 66 116672.0 67 82347.0 68 82347.0 69 82347.0 70 60820.0 71 39293.0 72 39293.0 73 39293.0 74 27975.5 75 16658.0 76 16658.0 77 16658.0 78 11603.5 79 6549.0 80 6549.0 81 6549.0 82 4509.5 83 2470.0 84 2470.0 85 2470.0 86 1629.0 87 788.0 88 788.0 89 788.0 90 525.5 91 263.0 92 263.0 93 263.0 94 156.0 95 49.0 96 49.0 97 49.0 98 30.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07777189082632079 2 0.018869488259478404 3 0.03021584721289025 4 0.03702366258493737 5 0.04575542577951953 6 0.012949648805524395 7 0.016920874439218542 8 0.03361975489891381 9 0.03391574687161151 10 0.018770824268579168 11 0.01800617833911011 12 0.013048312796423628 13 0.021114094052435965 14 0.03653034263044119 15 0.03292910696261918 16 0.020053456150269204 17 0.018968152250377637 18 0.02513465168157973 19 0.014010286707691154 20 0.012332998862404186 21 0.008386439226434846 22 0.017019538430117775 23 0.01588490253477659 24 0.016526218475621607 25 0.10867838597550569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4054164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.12312489524673 #Duplication Level Percentage of deduplicated Percentage of total 1 88.49302603805947 71.78830803643818 2 8.74385887397461 14.186583109997075 3 1.476328116155023 3.592930504596248 4 0.4744001982379131 1.5393930612793614 5 0.21212188017290667 0.8603994889140635 6 0.13987284408287096 0.680815331999287 7 0.09110466295343084 0.517348646591737 8 0.061193359430927026 0.3971357231899854 9 0.04335915568255982 0.31656871816098725 >10 0.24545340547936795 3.489421517353329 >50 0.01275641745103228 0.7066543746719327 >100 0.0055796846691972945 0.8044335394504132 >500 6.09912090006745E-4 0.35490548866711813 >1k 2.744603679144895E-4 0.48765478920373817 >5k 6.099119286988365E-5 0.27744766948659666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 5971 0.14728067241483078 No Hit GTATCAACGCAGAGTACTTTTTTTT 5244 0.1293484920688951 No Hit GGTATCAACGCAGAGTACTTTTTTT 4267 0.10524981229175731 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.466599772480837E-5 0.0 0.0 0.0 6 0.0 2.466599772480837E-5 0.0 0.0 0.0 7 0.0 2.466599772480837E-5 0.0 7.399799317442511E-5 0.0 8 0.0 2.466599772480837E-5 0.0 7.399799317442511E-5 0.0 9 0.0 2.466599772480837E-5 0.0 7.399799317442511E-5 0.0 10 0.0 2.466599772480837E-5 0.0 9.866399089923348E-5 0.0 11 0.0 2.466599772480837E-5 0.0 1.4799598634885022E-4 0.0 12 0.0 2.466599772480837E-5 0.0 1.4799598634885022E-4 0.0 13 0.0 2.466599772480837E-5 0.0 1.4799598634885022E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGAT 50 0.0015015443 13.298506 7 GTCGTAG 105 3.443054E-6 10.864235 1 GGTATCA 3385 0.0 9.9976635 1 TACACGG 105 4.0981206E-5 9.954702 5 TGGTATC 1640 0.0 9.559684 2 GTGGTAT 1705 0.0 9.478329 1 GCGTCAG 195 1.6661943E-9 9.262456 1 ACACCGT 150 2.708579E-6 8.86567 6 TAGACTG 410 0.0 8.8069315 5 CGTCAGA 205 4.132744E-9 8.806497 2 GTTCTAG 400 0.0 8.79324 1 TAGGGTG 440 0.0 8.638378 5 GAACCGT 100 0.0029094464 8.549039 6 TCTAGAC 395 0.0 8.419581 3 CTAGACT 375 0.0 8.36195 4 TACTGCG 125 2.7484586E-4 8.35906 7 TAGACAG 705 0.0 8.356559 5 TTAGGAC 330 0.0 8.350329 3 TAGGACT 460 0.0 8.262796 4 TATACAG 745 0.0 8.162977 5 >>END_MODULE