FastQCFastQC Report
Wed 25 May 2016
SRR1295336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054164
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142730.3520577855261899No Hit
GGTATCAACGCAGAGTACTTTTTTT120240.29658395664309584No Hit
TATCAACGCAGAGTACTTTTTTTTT82150.20263117130930075No Hit
GTGGTATCAACGCAGAGTACATGGG63010.15542045166401752No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55520.13694561936813607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3200.013.367623
CAGCGTC3350.013.0528282
CTGTCCG907.4964773E-611.6072449
GGTATCA43150.011.3596571
GCAGCGT4050.010.3403791
GTGGTAT18000.010.2581261
TGGTATC17300.010.0003882
CCGTGCA1554.0905434E-89.8031869
GAACCGT1106.8660294E-59.4969536
ACACGGT2054.2564352E-109.2653216
ATACCGT1054.5098478E-49.0447186
TATACCG950.00181496619.0053335
GGACCGT950.0018301088.9971146
AGAACCG1304.341943E-58.7744265
GCGTCAG5000.08.7453944
GTCTAGG3550.08.5794691
CTAACAC5750.08.4312883
TAGACAG6900.08.4035275
GTACTAG3200.08.3281171
GTATCAA74950.08.2543051
Produced by FastQC (version 0.11.5)