FastQCFastQC Report
Wed 25 May 2016
SRR1295335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417494
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA621352.5702235455393065No Hit
GCTTATGTACTCTGCGTTGATACCA369611.5288972795795976No Hit
CTTATGTACTCTGCGTTGATACCAC235510.9741906288081791No Hit
GAGTACATAAGCAGTGGTATCAACG160790.665110233986103No Hit
TATGTACTCTGCGTTGATACCACTG154830.6404566050629288No Hit
CCCATGTACTCTGCGTTGATACCAC141570.5856064172237863No Hit
GCGTTGATACCACTGCTTATGTACT125540.5192980830562558No Hit
GTACATGATAAGCAGTGGTATCAAC111590.46159369992231625No Hit
CATGTACTCTGCGTTGATACCACTG108640.4493909809083291No Hit
ATCATGTACTCTGCGTTGATACCAC106440.4402906480843386No Hit
CATAAGCAGTGGTATCAACGCAGAG102080.4222554430331575No Hit
ACTCTGCGTTGATACCACTGCTTAT101560.4201044552747597No Hit
GTATCAACGCAGAGTACTTTTTTTT97960.40521300156277534No Hit
CTGCTTATGTACTCTGCGTTGATAC86830.35917359050322356No Hit
ACGCAGAGTACATAAGCAGTGGTAT84370.3489977638000343No Hit
GTATCAACGCAGAGTACATAAGCAG82820.34258616567404093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA81110.3355127251608484No Hit
GTGGTATCAACGCAGAGTACATGGG71200.2945198623036913No Hit
TATCAACGCAGAGTACATAAGCAGT69640.2880668990284981No Hit
GGTATCAACGCAGAGTACATAAGCA66680.27582281486531096No Hit
GTACATGGGAAGCAGTGGTATCAAC63680.26341327010532395No Hit
GGTATCAACGCAGAGTACTTTTTTT62130.25700167197933066No Hit
AAGCAGTGGTATCAACGCAGAGTAC61720.25530570086213245No Hit
TATCAACGCAGAGTACTTTTTTTTT52780.2183252574773712No Hit
GTACTGGTTCACTATCGGTCAGTCA49050.20289605682578737No Hit
GCAGTGGTATCAACGCAGAGTACAT48910.202316944736988No Hit
GTACTCTGCGTTGATACCACTGCTT47570.1967740147441938No Hit
GTGGTATCAACGCAGAGTACATAAG46690.1931338816145976No Hit
GAGTACATGATAAGCAGTGGTATCA43600.180352050511811No Hit
ACATAAGCAGTGGTATCAACGCAGA39540.16355779993662858No Hit
GTACATGGGATAAGCAGTGGTATCA36040.14907999771664376No Hit
GCTTATCATGTACTCTGCGTTGATA34710.14357843287304953No Hit
GATACCACTGCTTATGTACTCTGCG33640.13915236190865418No Hit
CCACTGCTTATGTACTCTGCGTTGA32840.13584314997265762No Hit
GCTTATCCCATGTACTCTGCGTTGA32640.1350158469886585No Hit
GGATACCACGTGTCCCGCCCTACTC32550.1346435606458589No Hit
ATACAGGGTGACAGCCCCGTACACA32340.1337748925126598No Hit
GCGTTGATACCACTGCTTATCATGT30250.12512957632986887No Hit
GTTGATACCACTGCTTATGTACTCT30150.1247159248378693No Hit
ATACCACTGCTTATGTACTCTGCGT29440.12177899924467236No Hit
GATAAGCAGTGGTATCAACGCAGAG29360.12144807805107272No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28130.11636016469947805No Hit
CAGTGGTATCAACGCAGAGTACATA26970.11156180739228308No Hit
GAGTACATGGGAAGCAGTGGTATCA26720.11052767866228416No Hit
GGTATCAACGCAGAGTACATGGGAA25490.1054397653106895No Hit
GTATCAACGCAGAGTACATGGGAAG25100.1038265244918912No Hit
ACGCAGAGTACATGATAAGCAGTGG25050.1036196987458914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGT2950.014.16680710
TAGGACT751.474722E-512.6705594
ACAGTCC1301.9826984E-1012.42097958
CACCCGA1155.34601E-912.388700513
GCCTAGT1001.4275065E-712.3607041
CTAGTTC1850.012.3278563
GTGCTAC752.0580967E-411.4098811
GCTTACG1251.8100764E-811.4098811
TAGTTCC1501.7644197E-1011.4035024
CGTTAGC752.0692099E-411.4032662
TATACTT2350.011.3226265
TAGAACT1950.011.2085714
GTACTAG1201.261069E-711.0929391
GTCTTAG1201.261069E-711.0929391
TCTAACC600.00586473711.086513
CCGAGTT1553.1468517E-1011.02879816
CCTCTAG951.3479446E-511.0095331
GTACAAA5600.010.6967621
GAACAAA13950.010.5647041
CGCAAAA1951.4551915E-1110.2337022
Produced by FastQC (version 0.11.5)