##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295335_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2417494
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.098295176740873	31.0	30.0	33.0	26.0	34.0
2	29.73215817702133	31.0	30.0	34.0	25.0	34.0
3	30.269520420733205	31.0	30.0	34.0	26.0	34.0
4	33.41564860140294	35.0	35.0	37.0	27.0	37.0
5	33.9686365302251	35.0	35.0	37.0	28.0	37.0
6	34.00755534450137	37.0	35.0	37.0	29.0	37.0
7	33.96086815520535	37.0	35.0	37.0	29.0	37.0
8	33.980596849464774	37.0	35.0	37.0	28.0	37.0
9	34.810199322107934	38.0	35.0	39.0	26.0	39.0
10	34.736207411476514	38.0	34.0	39.0	27.0	39.0
11	35.21281790151289	39.0	35.0	39.0	27.0	39.0
12	35.23607049283266	39.0	35.0	39.0	27.0	39.0
13	35.02461929584934	38.0	35.0	39.0	27.0	39.0
14	35.1701410634318	39.0	34.0	41.0	23.0	41.0
15	35.77805446466465	39.0	34.0	41.0	27.0	41.0
16	35.79348076975579	39.0	35.0	41.0	27.0	41.0
17	35.76073487669463	39.0	35.0	41.0	27.0	41.0
18	35.71106608744427	39.0	34.0	41.0	27.0	41.0
19	35.79396556930441	39.0	34.0	41.0	26.0	41.0
20	35.82549449967611	39.0	34.0	41.0	26.0	41.0
21	35.835760916056046	39.0	34.0	41.0	27.0	41.0
22	35.596765079871965	39.0	34.0	41.0	25.0	41.0
23	35.28870971344706	39.0	34.0	40.0	24.0	41.0
24	35.628834239092214	39.0	34.0	41.0	26.0	41.0
25	34.47114698113005	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6153079706684252
1101	2	0.7560480053493386
1101	3	0.6063943199678086
1101	4	0.48618299280028054
1101	5	0.33580799837638153
1101	6	0.3662222533401831
1101	7	0.4256916109614721
1101	8	0.3804483822416529
1101	9	0.4157353738934546
1101	10	0.7966443804696581
1101	11	0.7474784985012448
1101	12	0.6572657941089801
1101	13	0.6469410465315875
1101	14	1.3064298128348568
1101	15	0.9547566851926135
1101	16	0.7770532618053281
1101	17	0.9748212269876575
1101	18	1.1214649162490176
1101	19	1.0280891052205519
1101	20	1.153707435593759
1101	21	1.1995536697130333
1101	22	1.0637098972696606
1101	23	-1.3487605366681734
1101	24	0.43732624982786206
1101	25	0.7914677721686019
1102	1	0.5078017334199885
1102	2	0.5173143257337003
1102	3	0.4066336044177028
1102	4	0.6221293483228223
1102	5	0.38174508288179965
1102	6	0.28945318422346844
1102	7	0.24444691056035595
1102	8	0.4257658338798649
1102	9	0.6265179509023326
1102	10	1.0360670120156215
1102	11	0.734006407053819
1102	12	0.7284631234404628
1102	13	0.7204861597104468
1102	14	1.343624152773586
1102	15	0.8975970856468152
1102	16	0.712080888679921
1102	17	1.0746813042089585
1102	18	0.9778761177140183
1102	19	1.0942422500073405
1102	20	1.0185542388339783
1102	21	1.1426538047189183
1102	22	0.9311170063015055
1102	23	1.0347807962415487
1102	24	1.0337646846203725
1102	25	0.8721532053250414
1103	1	-0.051544499008034705
1103	2	-1.3885409777739568
1103	3	-0.32606000759048115
1103	4	0.05829399809788782
1103	5	-0.17432642060254722
1103	6	-0.13316987890782173
1103	7	-0.054755008505665614
1103	8	0.20243890517611618
1103	9	0.7719271804255357
1103	10	0.864992527307038
1103	11	0.533321879908442
1103	12	0.35496995003116183
1103	13	0.23242854798940016
1103	14	-0.9770296371873073
1103	15	-0.06786731016796921
1103	16	-0.004211442290404932
1103	17	0.47443562782368787
1103	18	0.5396891890074684
1103	19	0.6462386907292554
1103	20	0.5793861158382256
1103	21	0.6808332751174788
1103	22	0.152898400075955
1103	23	1.106201091766863
1103	24	0.9407406233911573
1103	25	0.7939293377039931
1104	1	0.18662049120899127
1104	2	0.2721997884969731
1104	3	-0.059506871707622366
1104	4	-0.03294097763173909
1104	5	0.0011223853800004235
1104	6	0.002049585464902748
1104	7	0.1472710181466752
1104	8	0.1904346597021771
1104	9	-2.132722721155588
1104	10	-0.3814932913606697
1104	11	-0.25533794427497014
1104	12	0.1164100765352103
1104	13	0.32328837723957093
1104	14	-1.6292657576513037
1104	15	-0.33254058954619126
1104	16	0.07519485710682972
1104	17	0.4511987868192193
1104	18	0.5676272178137651
1104	19	0.448305840623469
1104	20	0.6023927432787346
1104	21	0.7011228522609727
1104	22	0.8084953734524873
1104	23	1.2533633818718855
1104	24	0.9033843117099778
1104	25	0.7687546081128716
1105	1	-0.0389940166976217
1105	2	0.13712440236628964
1105	3	-1.27601425157507
1105	4	-0.21939402320255397
1105	5	-0.20052333768826713
1105	6	0.1911652280419176
1105	7	-0.08017988796611775
1105	8	0.026040941699335463
1105	9	0.4799920815127834
1105	10	0.6454790327834559
1105	11	0.1519537111171516
1105	12	0.08790170811548847
1105	13	-0.0167790174922402
1105	14	0.7547597426679928
1105	15	0.47453387297360905
1105	16	0.10955877422718174
1105	17	0.5874088433543037
1105	18	0.459797450845123
1105	19	0.5428577991475692
1105	20	-0.10844308849419804
1105	21	0.33112108441201116
1105	22	0.48271418264386057
1105	23	0.7377134087504089
1105	24	0.6379697511286793
1105	25	0.8454521940970778
1106	1	-1.3225149845011792
1106	2	-0.8819992381313675
1106	3	-0.9963509507297097
1106	4	-1.0850634547370532
1106	5	-0.7155874445547283
1106	6	-0.7039617655420614
1106	7	-0.6293190203971903
1106	8	-0.6410080325667025
1106	9	-0.2240129498376504
1106	10	-1.7571126392955776
1106	11	-1.175637436074112
1106	12	-0.8955948739186184
1106	13	-0.9571373970931418
1106	14	-0.01930033822323196
1106	15	-0.7520236927586907
1106	16	-1.4216209359865815
1106	17	-0.5547560622708758
1106	18	-0.5520416939423285
1106	19	-0.4709840936424712
1106	20	-0.22491155409753105
1106	21	-0.21865849714401975
1106	22	-0.1810705059841098
1106	23	-0.5729316077586049
1106	24	-0.7113609567332446
1106	25	-1.3812645589093293
1107	1	0.22309251275820685
1107	2	0.36199847434425436
1107	3	0.12320817598213907
1107	4	0.21965237276334904
1107	5	0.09062667615665987
1107	6	0.19736426621773262
1107	7	0.021348811239150223
1107	8	0.06457488750768192
1107	9	0.6040602980583429
1107	10	0.31555528256608767
1107	11	0.2759957423884458
1107	12	0.12129104321495277
1107	13	0.29262712363452437
1107	14	-0.8930063640967489
1107	15	-0.2782399577236916
1107	16	0.0013278021690439346
1107	17	0.13101614988491406
1107	18	0.4516126719255098
1107	19	0.537810551364565
1107	20	0.488389477075323
1107	21	0.5547804345369016
1107	22	-0.037737167575933483
1107	23	0.861216688853851
1107	24	0.6341992163038555
1107	25	0.28412642642729935
1108	1	-6.874759332475833E-5
1108	2	0.3030077141800973
1108	3	-0.03238538157340187
1108	4	-0.33455200848992206
1108	5	-0.32257459769649444
1108	6	-0.20722472010731963
1108	7	-0.12181424778913197
1108	8	-0.14425084213991113
1108	9	0.40595292424058727
1108	10	0.3219534785523166
1108	11	0.08685493897008456
1108	12	0.05766759991387005
1108	13	-0.12955662902869136
1108	14	0.23753144666731885
1108	15	-0.008683100614966577
1108	16	-0.07338117334342797
1108	17	0.22682506626684074
1108	18	0.3493249911549725
1108	19	0.33076316392971705
1108	20	0.4328135085770555
1108	21	0.3789557406038213
1108	22	0.2835060516243857
1108	23	0.16166254772136313
1108	24	0.13269292074277672
1108	25	-0.6297182400021981
1109	1	-0.7421749616335163
1109	2	-0.7344430790952536
1109	3	-0.7743779630277992
1109	4	-0.7134800308305813
1109	5	-1.0113739127399768
1109	6	-1.1912530742987286
1109	7	-0.8314305347994306
1109	8	-1.1369899379079271
1109	9	-0.9759934466289266
1109	10	-1.0752131188084633
1109	11	-1.1710192269749058
1109	12	-1.1762742354622162
1109	13	-1.1669104122307985
1109	14	-0.5224330683558875
1109	15	-1.2387730093328457
1109	16	-1.4106931005139316
1109	17	-1.3264157514299768
1109	18	-1.9025846902868366
1109	19	-2.2347606005407243
1109	20	-1.9057805320614492
1109	21	-1.5803116098930303
1109	22	-1.5866540040964594
1109	23	-1.200909331020405
1109	24	-2.0822473390371172
1109	25	-1.1048780749978704
1110	1	-0.008186568091772983
1110	2	0.13266916227156855
1110	3	0.00790555157781725
1110	4	0.14557291780756998
1110	5	0.14216564909290952
1110	6	0.21196666056540892
1110	7	0.07148838233444366
1110	8	0.04140545203260615
1110	9	0.4009808431678863
1110	10	0.24295962993574705
1110	11	0.07183704569916216
1110	12	0.2699702050292956
1110	13	0.20197551596534424
1110	14	-2.567870351605478
1110	15	-0.7180333751260974
1110	16	-0.1656577724685846
1110	17	-0.23553299847043263
1110	18	-2.8076838421972923
1110	19	-0.44406535914975365
1110	20	-1.8158744654502996
1110	21	-0.1496929021937916
1110	22	-1.4763349985488077
1110	23	-0.4239054589634037
1110	24	0.2268520365440878
1110	25	0.07092845091532496
1111	1	-0.3013889711142035
1111	2	-1.421660379393554
1111	3	-0.4125013574949712
1111	4	-0.1502537983130452
1111	5	-0.10228470807686563
1111	6	-0.0292001749652826
1111	7	-0.05325822579837336
1111	8	-0.1588293818269122
1111	9	-1.5173623260159843
1111	10	-0.25886256838003874
1111	11	-0.09093035251288484
1111	12	-0.15610510534619237
1111	13	-0.04067621571078206
1111	14	0.846239803106343
1111	15	0.2618636071693743
1111	16	0.27499799916762413
1111	17	-0.06805550764083534
1111	18	0.10595044814576937
1111	19	-0.3437300373519889
1111	20	0.18624874949934167
1111	21	-0.7584131215424108
1111	22	-0.31786360343381403
1111	23	-0.011739646838158535
1111	24	-0.5788675350310513
1111	25	-2.1029296087539393
1112	1	0.019476077414534387
1112	2	0.3610296679803504
1112	3	0.040875380531954164
1112	4	0.40795553994073686
1112	5	0.10740230593000177
1112	6	0.1592630217211095
1112	7	0.14410130775087282
1112	8	0.04192074777125754
1112	9	-0.2573948153786674
1112	10	0.5393176680275502
1112	11	0.07878345366984973
1112	12	0.1553033829489081
1112	13	0.05753199337084425
1112	14	0.45566197491501725
1112	15	0.17628518872896848
1112	16	0.2156358787876016
1112	17	-3.707553693266469
1112	18	-0.9979493018965755
1112	19	-1.0550784742184334
1112	20	-0.029173689204576192
1112	21	-0.33961599785186536
1112	22	-1.7593848036411757
1112	23	-1.4867492704645429
1112	24	-0.2229296292348053
1112	25	-0.018487034857322726
1113	1	-0.3421301590382235
1113	2	-0.16972969148351424
1113	3	-0.08234749726300095
1113	4	-0.4573564573777844
1113	5	-0.1702588774527598
1113	6	-0.1664331818359983
1113	7	-0.23429958479117374
1113	8	-0.23452032941580825
1113	9	0.25669406313627263
1113	10	0.3998826560555955
1113	11	-0.04146643582566867
1113	12	-0.007783765468360571
1113	13	-0.4794025793679424
1113	14	-1.6821590133925213
1113	15	-1.9416542330427191
1113	16	-0.5900053434096932
1113	17	-0.3245990793435638
1113	18	-0.6258891894832601
1113	19	-0.15868838858441592
1113	20	0.31851898225964703
1113	21	-1.5714276941627645
1113	22	-2.089951811888632
1113	23	-0.6551963293238146
1113	24	-2.523301698967309
1113	25	-0.5315888412575163
1114	1	-0.03547720812611033
1114	2	0.2186701919835059
1114	3	-0.06300808293891436
1114	4	0.3388660154009173
1114	5	0.05082725399553567
1114	6	0.1596521192021001
1114	7	0.21096373289670822
1114	8	0.02113115681804345
1114	9	0.5862992763588082
1114	10	0.35598952771876924
1114	11	0.23402108259553245
1114	12	0.2236168622365824
1114	13	0.15038301949057598
1114	14	1.0299675807007205
1114	15	0.3151434196644516
1114	16	0.14217346507316364
1114	17	-0.4515844548555563
1114	18	-0.12897310500136427
1114	19	-0.4989869805443945
1114	20	-1.6873794351513638
1114	21	-0.8188157848029789
1114	22	-1.4936727008759405
1114	23	-2.9612145111738783
1114	24	-2.0804426880898106
1114	25	-1.534533227822358
1115	1	0.12325556300314133
1115	2	0.11660927884394923
1115	3	-0.0648956627910664
1115	4	0.2665842739180846
1115	5	0.1693310353429851
1115	6	0.12390732765305046
1115	7	0.31091335065382
1115	8	0.11998688409509839
1115	9	0.3149317639874738
1115	10	0.5781142983417737
1115	11	0.2467167954098599
1115	12	0.23289470952543923
1115	13	0.08592219943505341
1115	14	0.8404120786446683
1115	15	0.3943938329353571
1115	16	0.20792159034722602
1115	17	0.15456374803956408
1115	18	0.47230343227263205
1115	19	-0.3107219732639095
1115	20	0.3298985228789135
1115	21	-0.6937686909933873
1115	22	0.4132078625886564
1115	23	0.48505044737017045
1115	24	0.62135694929583
1115	25	0.7132040047316934
1116	1	-0.5068855247981681
1116	2	-0.21157635280370712
1116	3	-0.2424492030598948
1116	4	0.05827841095105413
1116	5	-0.04099321716210369
1116	6	-0.1711780873391504
1116	7	-0.1343380343425622
1116	8	-0.15652394405840653
1116	9	-0.07704460743818942
1116	10	-0.29402139980865627
1116	11	-0.25563249831127166
1116	12	-0.2431637289371622
1116	13	-0.8094649066678912
1116	14	0.06614346227488
1116	15	-0.11433229514135945
1116	16	-0.2503927411913267
1116	17	-0.06618552597289096
1116	18	-0.07544167408283187
1116	19	0.18378003413747734
1116	20	-0.1532390114817872
1116	21	-0.6903797226445647
1116	22	-0.6715489606304033
1116	23	-1.7250891762781109
1116	24	-1.2852812489728862
1116	25	-2.0140202270848846
1201	1	0.5495038711537461
1201	2	0.7542699156019417
1201	3	0.5901136053983116
1201	4	0.282493597937453
1201	5	0.3979197601072144
1201	6	0.24440132288751215
1201	7	0.30152656038467995
1201	8	0.36229088083557315
1201	9	0.5954889254625826
1201	10	0.9366155514097372
1201	11	0.5725091855210209
1201	12	0.5395773544746234
1201	13	0.5421744718943842
1201	14	1.1908080362731184
1201	15	0.7403489535093897
1201	16	0.5382906097348652
1201	17	0.542334051986856
1201	18	0.5782810522464104
1201	19	0.809183009525114
1201	20	0.8976152838693423
1201	21	0.8377429919345474
1201	22	0.8982147980363564
1201	23	0.4218803434991045
1201	24	0.8684452878345326
1201	25	0.8504147371977737
1202	1	0.57749941372359
1202	2	0.8506687798752957
1202	3	0.6980904940845747
1202	4	0.5841340012171443
1202	5	0.4253860022962428
1202	6	0.3098827301418794
1202	7	0.272616541838417
1202	8	0.32650533851598595
1202	9	0.827818597909669
1202	10	0.021381931162402168
1202	11	0.2767211691565308
1202	12	0.5010778834508329
1202	13	0.4787200179846849
1202	14	1.2623574017026442
1202	15	0.7787039107471827
1202	16	0.6353999190808537
1202	17	-1.4177710663945078
1202	18	0.15023750698156846
1202	19	0.5513383875839395
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2210	24	1.1178293688311456
2210	25	1.074431878463038
2211	1	0.033780285847210934
2211	2	-0.7611140674301495
2211	3	0.019002057933992234
2211	4	0.3397417104500846
2211	5	0.32410522528571306
2211	6	0.0991894032762417
2211	7	0.14589261709189572
2211	8	0.3565269925960166
2211	9	0.6382055021054285
2211	10	0.7012831879233588
2211	11	0.44874032493274996
2211	12	0.282802061832264
2211	13	0.0962417513256284
2211	14	-0.9036549661694195
2211	15	-0.0038818337029695726
2211	16	0.25332073109300524
2211	17	0.1992485490949747
2211	18	0.2799059744560637
2211	19	0.5665212107940718
2211	20	0.6560754713142174
2211	21	0.5875296204099172
2211	22	-0.08763261807733613
2211	23	0.5768531337596983
2211	24	-0.17147135571580918
2211	25	0.47740007936621964
2212	1	-0.12093339533921466
2212	2	-1.0496436504723121
2212	3	-0.38870350926843145
2212	4	-0.01041629871211569
2212	5	0.08576975669343057
2212	6	-0.0696464923678306
2212	7	0.0402858153215746
2212	8	0.09725336886747016
2212	9	-2.1578638743989558
2212	10	-0.05337458830150155
2212	11	-0.05156598766915721
2212	12	-0.053799770608144115
2212	13	-0.026558155499436964
2212	14	0.5591264997829057
2212	15	0.14483235218102664
2212	16	0.20963197420194035
2212	17	0.38534199579115125
2212	18	0.08947309923282631
2212	19	-0.4447411154734979
2212	20	0.3441199623461273
2212	21	0.3297103021398655
2212	22	-1.6424394741997048
2212	23	-0.2019414155065462
2212	24	0.3308205535939237
2212	25	-0.3059497536953586
2213	1	0.04082761095009246
2213	2	0.40910905008940546
2213	3	-0.006546900693845004
2213	4	0.23278116636061696
2213	5	0.1418435000320457
2213	6	0.2325427629972836
2213	7	0.14290043916333417
2213	8	0.20478901628212753
2213	9	0.4846364856759351
2213	10	0.5881156416663273
2213	11	-0.0637019519497315
2213	12	-0.048810572855011
2213	13	-0.016584560991773856
2213	14	-1.8832567159259597
2213	15	-0.5210008611273409
2213	16	-0.24726081949914658
2213	17	-2.4057694353865386
2213	18	-1.0288734368466734
2213	19	-0.42122774469237356
2213	20	0.22546808275517094
2213	21	-0.18162581577266224
2213	22	0.37702473435567185
2213	23	0.8072390540311147
2213	24	0.6121168232180025
2213	25	-1.0844268769369876
2214	1	-0.020041954267298934
2214	2	0.0248300097515326
2214	3	-0.00826013942736381
2214	4	0.3098604116582848
2214	5	0.2043251827209005
2214	6	0.23516667536144098
2214	7	0.23233355841460934
2214	8	0.08617804588678268
2214	9	0.49662158727272043
2214	10	0.3984567949145372
2214	11	-0.10778635811948334
2214	12	-0.1966677143257911
2214	13	-0.05751421669425838
2214	14	0.7745528150926475
2214	15	0.30845395746577253
2214	16	0.3022967507180212
2214	17	0.25784049662348707
2214	18	0.07002354562499136
2214	19	-0.9396863538771356
2214	20	0.07388983411332362
2214	21	0.34927614318491607
2214	22	0.638667751068823
2214	23	1.0077900775684938
2214	24	0.8102496705481883
2214	25	0.7863663524025384
2215	1	-0.5609130330882017
2215	2	-0.05973807220080474
2215	3	-0.17636267146032836
2215	4	0.25616957579730126
2215	5	-2.840719023993188E-4
2215	6	0.02488525796469787
2215	7	-0.03160311188337772
2215	8	-0.09193033476325496
2215	9	-0.006811770026004638
2215	10	0.36016302438877545
2215	11	0.08611501467906635
2215	12	-0.09465794096136193
2215	13	-0.26378790409131625
2215	14	0.5287112083892396
2215	15	0.01820016997172047
2215	16	0.04386256027350299
2215	17	0.19633793976211678
2215	18	0.009230722761081722
2215	19	-0.6850422797344606
2215	20	-2.140004050331015
2215	21	-2.4856937407522324
2215	22	-0.592792365146714
2215	23	0.28446119874374176
2215	24	0.38882275403072697
2215	25	-0.6361220117704676
2216	1	-0.5207624009841751
2216	2	-0.8655195894949514
2216	3	-0.5544481020396219
2216	4	-0.2251871486087964
2216	5	-0.17793956675992462
2216	6	-0.36211750975999735
2216	7	-0.2548225890717646
2216	8	-0.3814051430035832
2216	9	-0.2030605646743453
2216	10	-2.330773090715205
2216	11	-1.0038647289414655
2216	12	-0.696830486196653
2216	13	-0.4909328188196582
2216	14	-1.2869380492004296
2216	15	-1.2111224673412906
2216	16	-0.5654988783968378
2216	17	-0.236154170173144
2216	18	-0.43010246340129754
2216	19	-1.905004916529741
2216	20	-1.6297583635304491
2216	21	-1.431252545733706
2216	22	-1.4451136513153529
2216	23	-4.954753555551481
2216	24	-2.8329111834225813
2216	25	-2.3312919762409052
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	16.0
4	32.0
5	42.0
6	52.0
7	271.0
8	119.0
9	383.0
10	861.0
11	1751.0
12	3553.0
13	6375.0
14	10205.0
15	15075.0
16	19593.0
17	22395.0
18	22722.0
19	21864.0
20	21319.0
21	20208.0
22	19304.0
23	19107.0
24	19671.0
25	20485.0
26	22471.0
27	25793.0
28	30108.0
29	37276.0
30	46136.0
31	58278.0
32	78547.0
33	107456.0
34	147958.0
35	207501.0
36	298002.0
37	442549.0
38	670014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.74306977545236	19.928261339370067	12.97195452245849	20.35671436271908
2	13.649291060368473	23.666171560494664	39.44079205954514	23.243745319591724
3	19.31607804191753	30.735936109904205	30.702832433327128	19.24515341485114
4	12.195240044180494	20.55758631149566	35.07532327367697	32.17185037064687
5	10.175834318008272	44.19457038856549	33.448831737504634	12.180763555921605
6	27.90082644628099	36.22424725121277	21.826001510152153	14.048924792354079
7	23.02654635112317	38.10801339088994	21.014354534196546	17.851085723790344
8	22.141298752034007	37.91369187042607	23.058898896380253	16.88611048115967
9	26.416120336397636	21.126945331659236	19.359293435842854	33.09764089610027
10	15.07345274231295	30.406257551332512	29.89098772256777	24.62930198378677
11	28.42401563041775	29.429011438969955	21.4912037523595	20.6557691782528
12	23.581674208425614	29.852994658225303	26.26104319137836	20.304287941970724
13	27.426844906111477	23.975472835615115	28.877885125844678	19.71979713242873
14	25.274029464323405	26.527947570422448	24.935708710590287	23.26231425466386
15	28.209272885807774	27.42762812944481	24.11358347786395	20.249515506883466
16	22.098398451497154	29.069888686875878	28.277555397954142	20.55415746367283
17	20.02890590767074	33.18593499605058	26.701883931981328	20.08327516429736
18	20.457003774640473	27.221563368194207	33.3326574677219	18.988775389443422
19	21.579483708300526	33.10227239345841	25.78938810500929	19.528855793231763
20	21.70947455523376	29.659702110053786	29.032809267687217	19.59801406702524
21	22.204276096546664	31.2883851838	25.884289136907658	20.623049582745683
22	21.397392018165508	29.867416280770975	24.198101029091955	24.53709067197156
23	23.285296989556954	26.96841434553068	25.61388047597769	24.132408188934676
24	19.831537171590373	30.784641400000744	24.465092454713925	24.918728973694957
25	23.002761608466116	30.472686826808765	24.898246177787293	21.626305386937826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9664.0
1	9664.0
2	9016.5
3	8369.0
4	8369.0
5	8369.0
6	11693.0
7	15017.0
8	15017.0
9	15017.0
10	15531.0
11	16045.0
12	16045.0
13	16045.0
14	20022.0
15	23999.0
16	23999.0
17	23999.0
18	33490.0
19	42981.0
20	42981.0
21	42981.0
22	57925.0
23	72869.0
24	72869.0
25	72869.0
26	94013.5
27	115158.0
28	115158.0
29	115158.0
30	131930.5
31	148703.0
32	148703.0
33	148703.0
34	163516.5
35	178330.0
36	178330.0
37	178330.0
38	212106.5
39	245883.0
40	245883.0
41	245883.0
42	375071.5
43	504260.0
44	504260.0
45	504260.0
46	433826.0
47	363392.0
48	363392.0
49	363392.0
50	306713.5
51	250035.0
52	250035.0
53	250035.0
54	204757.0
55	159479.0
56	159479.0
57	159479.0
58	133713.5
59	107948.0
60	107948.0
61	107948.0
62	92991.0
63	78034.0
64	78034.0
65	78034.0
66	59209.0
67	40384.0
68	40384.0
69	40384.0
70	31520.5
71	22657.0
72	22657.0
73	22657.0
74	16079.5
75	9502.0
76	9502.0
77	9502.0
78	6327.5
79	3153.0
80	3153.0
81	3153.0
82	2150.0
83	1147.0
84	1147.0
85	1147.0
86	756.5
87	366.0
88	366.0
89	366.0
90	227.5
91	89.0
92	89.0
93	89.0
94	57.0
95	25.0
96	25.0
97	25.0
98	15.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08521220735191071
2	0.02084803519677815
3	0.03491218592476341
4	0.043143850615554785
5	0.053733328810743684
6	0.0214685124347775
7	0.025935948548372816
8	0.044757091434353094
9	0.0441779793455537
10	0.029038334738369566
11	0.02763191966557104
12	0.02097213064437802
13	0.030817036153967702
14	0.04748719128155023
15	0.04347477180915444
16	0.02928652563356931
17	0.028417857500370222
18	0.034746725327963586
19	0.021799433628377156
20	0.020434383704778587
21	0.015842852143583396
22	0.02506728041517373
23	0.023743595640775116
24	0.024033151685174812
25	0.10742529247228741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2417494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.02623293809878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4134658846857	26.764758556844654
2	11.321556575794	7.931029556912492
3	4.248809793859647	4.4645940464821035
4	2.148497646247646	3.0101511609770797
5	1.2254932223486499	2.1462205535022547
6	0.7975837786734808	1.6761813131679817
7	0.5981274813634064	1.4665106742239107
8	0.42606855417515277	1.1938861140910286
9	0.33917034781907696	1.0691873647566275
>10	2.1922807392052293	14.68698335907781
>50	0.1703075693468507	4.078630780050616
>100	0.09110504185167027	6.454958630554673
>500	0.013287841190750337	3.350207149662671
>1k	0.011372476694786325	8.203437146469373
>5k	0.0014365233719730096	3.693697725007309
>10k+	0.0014365233719730096	9.809565868219416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	62135	2.5702235455393065	No Hit
GCTTATGTACTCTGCGTTGATACCA	36961	1.5288972795795976	No Hit
CTTATGTACTCTGCGTTGATACCAC	23551	0.9741906288081791	No Hit
GAGTACATAAGCAGTGGTATCAACG	16079	0.665110233986103	No Hit
TATGTACTCTGCGTTGATACCACTG	15483	0.6404566050629288	No Hit
CCCATGTACTCTGCGTTGATACCAC	14157	0.5856064172237863	No Hit
GCGTTGATACCACTGCTTATGTACT	12554	0.5192980830562558	No Hit
GTACATGATAAGCAGTGGTATCAAC	11159	0.46159369992231625	No Hit
CATGTACTCTGCGTTGATACCACTG	10864	0.4493909809083291	No Hit
ATCATGTACTCTGCGTTGATACCAC	10644	0.4402906480843386	No Hit
CATAAGCAGTGGTATCAACGCAGAG	10208	0.4222554430331575	No Hit
ACTCTGCGTTGATACCACTGCTTAT	10156	0.4201044552747597	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9796	0.40521300156277534	No Hit
CTGCTTATGTACTCTGCGTTGATAC	8683	0.35917359050322356	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	8437	0.3489977638000343	No Hit
GTATCAACGCAGAGTACATAAGCAG	8282	0.34258616567404093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8111	0.3355127251608484	No Hit
GTGGTATCAACGCAGAGTACATGGG	7120	0.2945198623036913	No Hit
TATCAACGCAGAGTACATAAGCAGT	6964	0.2880668990284981	No Hit
GGTATCAACGCAGAGTACATAAGCA	6668	0.27582281486531096	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6368	0.26341327010532395	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6213	0.25700167197933066	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	6172	0.25530570086213245	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5278	0.2183252574773712	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4905	0.20289605682578737	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4891	0.202316944736988	No Hit
GTACTCTGCGTTGATACCACTGCTT	4757	0.1967740147441938	No Hit
GTGGTATCAACGCAGAGTACATAAG	4669	0.1931338816145976	No Hit
GAGTACATGATAAGCAGTGGTATCA	4360	0.180352050511811	No Hit
ACATAAGCAGTGGTATCAACGCAGA	3954	0.16355779993662858	No Hit
GTACATGGGATAAGCAGTGGTATCA	3604	0.14907999771664376	No Hit
GCTTATCATGTACTCTGCGTTGATA	3471	0.14357843287304953	No Hit
GATACCACTGCTTATGTACTCTGCG	3364	0.13915236190865418	No Hit
CCACTGCTTATGTACTCTGCGTTGA	3284	0.13584314997265762	No Hit
GCTTATCCCATGTACTCTGCGTTGA	3264	0.1350158469886585	No Hit
GGATACCACGTGTCCCGCCCTACTC	3255	0.1346435606458589	No Hit
ATACAGGGTGACAGCCCCGTACACA	3234	0.1337748925126598	No Hit
GCGTTGATACCACTGCTTATCATGT	3025	0.12512957632986887	No Hit
GTTGATACCACTGCTTATGTACTCT	3015	0.1247159248378693	No Hit
ATACCACTGCTTATGTACTCTGCGT	2944	0.12177899924467236	No Hit
GATAAGCAGTGGTATCAACGCAGAG	2936	0.12144807805107272	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2813	0.11636016469947805	No Hit
CAGTGGTATCAACGCAGAGTACATA	2697	0.11156180739228308	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2672	0.11052767866228416	No Hit
GGTATCAACGCAGAGTACATGGGAA	2549	0.1054397653106895	No Hit
GTATCAACGCAGAGTACATGGGAAG	2510	0.1038265244918912	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2505	0.1036196987458914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.27302983999133E-5	0.0	0.0	0.0	0.0
6	2.0682574599978324E-4	0.0	0.0	0.0	0.0
7	2.0682574599978324E-4	0.0	0.0	0.0	0.0
8	2.481908951997399E-4	0.0	0.0	0.0	0.0
9	2.481908951997399E-4	0.0	0.0	0.0	0.0
10	2.481908951997399E-4	0.0	0.0	0.0	0.0
11	2.481908951997399E-4	0.0	0.0	0.0	0.0
12	2.481908951997399E-4	0.0	0.0	4.136514919995665E-5	0.0
13	2.481908951997399E-4	0.0	0.0	8.27302983999133E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTGT	295	0.0	14.166807	10
TAGGACT	75	1.474722E-5	12.670559	4
ACAGTCC	130	1.9826984E-10	12.4209795	8
CACCCGA	115	5.34601E-9	12.3887005	13
GCCTAGT	100	1.4275065E-7	12.360704	1
CTAGTTC	185	0.0	12.327856	3
GTGCTAC	75	2.0580967E-4	11.409881	1
GCTTACG	125	1.8100764E-8	11.409881	1
TAGTTCC	150	1.7644197E-10	11.403502	4
CGTTAGC	75	2.0692099E-4	11.403266	2
TATACTT	235	0.0	11.322626	5
TAGAACT	195	0.0	11.208571	4
GTACTAG	120	1.261069E-7	11.092939	1
GTCTTAG	120	1.261069E-7	11.092939	1
TCTAACC	60	0.005864737	11.08651	3
CCGAGTT	155	3.1468517E-10	11.028798	16
CCTCTAG	95	1.3479446E-5	11.009533	1
GTACAAA	560	0.0	10.696762	1
GAACAAA	1395	0.0	10.564704	1
CGCAAAA	195	1.4551915E-11	10.233702	2
>>END_MODULE