FastQCFastQC Report
Wed 25 May 2016
SRR1295335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417494
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA651712.695808138510375No Hit
GCTTATGTACTCTGCGTTGATACCA421231.7424241797497737No Hit
CTTATGTACTCTGCGTTGATACCAC278091.1503234341015944No Hit
GTATCAACGCAGAGTACTTTTTTTT277171.1465178403751985No Hit
GGTATCAACGCAGAGTACTTTTTTT186880.7730319082487899No Hit
GAGTACATAAGCAGTGGTATCAACG179740.7434971917200208No Hit
CCCATGTACTCTGCGTTGATACCAC169580.7014702001328649No Hit
TATCAACGCAGAGTACTTTTTTTTT169520.7012220092376651No Hit
TATGTACTCTGCGTTGATACCACTG153330.6342518326829353No Hit
GCGTTGATACCACTGCTTATGTACT143020.59160436385778No Hit
CATAAGCAGTGGTATCAACGCAGAG127840.5288120673722458No Hit
CATGTACTCTGCGTTGATACCACTG127080.5256683160330491No Hit
ACTCTGCGTTGATACCACTGCTTAT124820.5163197923138588No Hit
ATCATGTACTCTGCGTTGATACCAC122570.5070126337438686No Hit
GTACTTTTTTTTTTTTTTTTTTTTT119240.4932380390602831No Hit
GTACATGATAAGCAGTGGTATCAAC110840.4584913137323195No Hit
ACGCAGAGTACATAAGCAGTGGTAT102190.422710459674357No Hit
CTGCTTATGTACTCTGCGTTGATAC102150.42254499907755716No Hit
ACGCAGAGTACTTTTTTTTTTTTTT93330.3860609374831954No Hit
GTATCAACGCAGAGTACATAAGCAG90740.3753473638404066No Hit
TATCAACGCAGAGTACATAAGCAGT79090.32715696502245717No Hit
GTGGTATCAACGCAGAGTACATGGG76530.3165674868272682No Hit
GGTATCAACGCAGAGTACATAAGCA70600.2920379533516939No Hit
AAGCAGTGGTATCAACGCAGAGTAC70000.28955604439969657No Hit
GTACATGGGAAGCAGTGGTATCAAC61610.25485068422093293No Hit
GCAGTGGTATCAACGCAGAGTACAT58080.24024878655334822No Hit
GTACTGGTTCACTATCGGTCAGTCA52400.21675338180777284No Hit
GTGGTATCAACGCAGAGTACATAAG52050.21530560158577436No Hit
GAGTACTTTTTTTTTTTTTTTTTTT51690.21381645621457593No Hit
ACATAAGCAGTGGTATCAACGCAGA49740.20575025212058437No Hit
GTACTCTGCGTTGATACCACTGCTT49500.20475748853978543No Hit
GAGTACATGATAAGCAGTGGTATCA45300.18738412587580364No Hit
GCTTATCATGTACTCTGCGTTGATA39850.16484011956182726No Hit
ATACAGGGTGACAGCCCCGTACACA39460.16322687874302894No Hit
CCACTGCTTATGTACTCTGCGTTGA39220.16223411516223No Hit
GATACCACTGCTTATGTACTCTGCG39060.16157227277503067No Hit
GTACATGGGATAAGCAGTGGTATCA37600.155532960991837No Hit
GGATACCACGTGTCCCGCCCTACTC37310.15433337166503824No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36310.15019685674504257No Hit
GCTTATCCCATGTACTCTGCGTTGA36100.14932818861184352No Hit
GCGTTGATACCACTGCTTATCATGT35510.14688764480904606No Hit
ATACCACTGCTTATGTACTCTGCGT33820.1398969345942534No Hit
GATAAGCAGTGGTATCAACGCAGAG33130.13704273929945637No Hit
CAGTGGTATCAACGCAGAGTACATA30750.12719783378986668No Hit
GTTGATACCACTGCTTATGTACTCT30400.12575005356786823No Hit
ACGCAGAGTACATGATAAGCAGTGG29790.12322677946667085No Hit
GAGTACATGGGAAGCAGTGGTATCA29660.12268903252707142No Hit
GGATAAGCAGTGGTATCAACGCAGA27960.11565695716307879No Hit
ACTCTGCGTTGATACCACTGCTTCC27360.11317504821108139No Hit
ACCCTGTATCGCGCGCCTTTCCAGA27170.11238911037628223No Hit
GGTATCAACGCAGAGTACATGGGAA26810.11089996500508378No Hit
TGATACCACTGCTTATGTACTCTGC26180.10829396060548652No Hit
CCCCATGTACTCTGCGTTGATACCA26070.10783894396428698No Hit
CAGTGGTATCAACGCAGAGTACATG25870.10701164098028786No Hit
GTATCAACGCAGAGTACATGGGAAG25840.10688754553268799No Hit
GTGGTATCAACGCAGAGTACTTTTT25810.10676345008508811No Hit
TACCACTGCTTATGTACTCTGCGTT25740.10647389404068841No Hit
ATCAACGCAGAGTACTTTTTTTTTT25520.10556386075828937No Hit
TATCAACGCAGAGTACATGATAAGC25160.10407471538709094No Hit
GCAGAGTACATAAGCAGTGGTATCA25020.10349560329829154No Hit
GCGTTGATACCACTGCTTCCCATGT24380.1008482337494943No Hit
CCCATATTCAGACAGGATACCACGT24290.1004759474066947No Hit
ATCATTAACTGAATCCATAGGTTAA24200.1001036610638951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG456.785001E-414.77180910
CGTATAC653.345509E-614.6273323
ACGCCCC400.005295847514.2436558
GACACGC500.001504540313.2946299
GCGGTCG655.475646E-513.1485349
GTGTAAC701.0707981E-412.2392241
ACTCGAG701.09550696E-412.209352514
GGACTTA1701.8189894E-1211.7307976
GACTGCG1751.8189894E-1211.3949247
GTCTAGC855.2159525E-511.199291
GGACTGC1701.2732926E-1111.1721886
ACCGATT600.00589677111.0783998
TCTGTCG1553.1650416E-1011.0273478
CCTGGAC1408.252755E-910.8660183
CAGGACT2400.010.6962384
TAGGACC909.440638E-510.5641844
ACAGCGA909.562248E-510.5508568
CTCTGTC5000.010.4453477
GGGGCGA1106.0807743E-610.3590228
GTACAAA2850.010.3544311
Produced by FastQC (version 0.11.5)