FastQCFastQC Report
Wed 25 May 2016
SRR1295334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950524
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG64840.13097603405215286No Hit
GTATCAACGCAGAGTACTTTTTTTT59820.12083569335286526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT456.773991E-414.7754848
TTACGCT855.3156782E-511.1790284
GACCGCT1002.4053988E-510.4483787
TAAGCGT951.6416784E-410.0022874
CGTCAGA1856.475602E-109.7582982
GGTATCA42700.09.5752241
GGACCGT1106.854438E-59.4985256
TATACTG10500.09.0496885
GCGTCAG1901.043918E-89.0079771
CTTACGC850.0074274488.9429513
GTATTAG8250.08.6440181
TTAGACT4900.08.3386424
GTATTAT8250.08.2982581
CCAGTAC5400.08.2701593
GGACTGT4850.08.2255276
GTAGGAC2701.3278623E-108.0941983
TAGGACT5700.08.001834
TCTAGAC4400.07.99022153
TAGAACG1553.890502E-57.96956444
GTGGTAT23000.07.93746381
Produced by FastQC (version 0.11.5)