##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295334_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4950524
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.032065898478624	31.0	30.0	33.0	26.0	34.0
2	30.023160578556936	31.0	30.0	34.0	25.0	34.0
3	30.405818252774857	31.0	30.0	34.0	26.0	34.0
4	33.79709885256591	35.0	35.0	37.0	28.0	37.0
5	33.97553248908601	35.0	35.0	37.0	28.0	37.0
6	34.141055775105826	36.0	35.0	37.0	30.0	37.0
7	34.06213705862248	36.0	35.0	37.0	29.0	37.0
8	34.082431677939546	36.0	35.0	37.0	29.0	37.0
9	35.113756442752326	38.0	35.0	39.0	27.0	39.0
10	35.004831811743564	38.0	35.0	39.0	27.0	39.0
11	35.409493015284845	38.0	35.0	39.0	28.0	39.0
12	35.4072879961798	38.0	35.0	39.0	28.0	39.0
13	35.319739486163485	38.0	35.0	39.0	27.0	39.0
14	35.48457355221387	39.0	34.0	41.0	25.0	41.0
15	36.10629703037497	39.0	35.0	41.0	27.0	41.0
16	36.20932248788209	39.0	36.0	41.0	27.0	41.0
17	36.018140705913154	39.0	35.0	41.0	27.0	41.0
18	35.99144454203232	39.0	35.0	41.0	27.0	41.0
19	36.04973352315836	39.0	35.0	41.0	27.0	41.0
20	36.01619727527834	39.0	35.0	41.0	27.0	41.0
21	36.004124613879256	39.0	34.0	41.0	27.0	41.0
22	35.750980098268386	39.0	34.0	41.0	26.0	41.0
23	35.423325490392536	39.0	34.0	40.0	25.0	41.0
24	35.754194303471714	39.0	34.0	41.0	27.0	41.0
25	34.42653403962893	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18241292182521818
1101	2	0.39426582993490555
1101	3	0.23257220687921176
1101	4	0.24739223656225562
1101	5	0.1430038131115552
1101	6	0.17311223441453905
1101	7	0.09662022651517788
1101	8	0.06947840843778863
1101	9	0.017702002904314895
1101	10	0.36042026225690904
1101	11	0.2957502092873199
1101	12	0.24893899151528132
1101	13	0.28689511697825054
1101	14	0.7520461667207314
1101	15	0.5803381849390092
1101	16	0.37767362691148065
1101	17	0.5518334788641113
1101	18	0.686134820542847
1101	19	0.6024411525892504
1101	20	0.7442848774011139
1101	21	0.6898600977677773
1101	22	0.46584121281553337
1101	23	-1.7256309174663187
1101	24	-0.09087558839341625
1101	25	0.24721074756235595
1102	1	0.16712754194465163
1102	2	0.14894499016727636
1102	3	0.16099397544119043
1102	4	0.3060774374762971
1102	5	0.0693388252408198
1102	6	0.07088787848596212
1102	7	0.040501955834656655
1102	8	0.07923762864201223
1102	9	0.21371917556584918
1102	10	0.7461464197584107
1102	11	0.4399122781453073
1102	12	0.323311289863625
1102	13	0.27204517376241455
1102	14	0.7416250921325656
1102	15	0.5387425160833246
1102	16	0.259156559622177
1102	17	0.6705605177177532
1102	18	0.6246894695428082
1102	19	0.6037384037178128
1102	20	0.7247422800176366
1102	21	0.7652813732147195
1102	22	0.5755151197146589
1102	23	0.6269131903571861
1102	24	0.6983443974413248
1102	25	0.35741644941730044
1103	1	-0.4276685670931748
1103	2	-1.670242021532367
1103	3	-0.4543121070032825
1103	4	-0.11215585727944699
1103	5	-0.3392047663413038
1103	6	-0.17235979153110748
1103	7	-0.09553832924264327
1103	8	0.04304075215457459
1103	9	0.7034950943298455
1103	10	0.7122450044345143
1103	11	0.45305797847988316
1103	12	0.2603707525020269
1103	13	0.07445631205946057
1103	14	-1.3387646553898875
1103	15	-0.418286718726975
1103	16	-0.2223596898661171
1103	17	0.2874661551383326
1103	18	0.47321382759461983
1103	19	0.4019225494152181
1103	20	0.4744933023089217
1103	21	0.5301886851868076
1103	22	0.11685259632785261
1103	23	0.9142552567429121
1103	24	0.6063128302094043
1103	25	0.343585116900023
1104	1	-0.1562059537068663
1104	2	-0.01097145624024165
1104	3	-0.47850034472384095
1104	4	-0.2853803630125995
1104	5	-0.29542046892407825
1104	6	-0.16302300676795056
1104	7	-0.17789393851226976
1104	8	-0.15677990599475322
1104	9	-2.289022816904847
1104	10	-0.7215883103855205
1104	11	-0.5678192856889837
1104	12	-0.1376878679481024
1104	13	0.12921486497429413
1104	14	-1.8537668922860675
1104	15	-0.628826041739984
1104	16	-0.22483775619636504
1104	17	0.14876543379286744
1104	18	0.27758616401278857
1104	19	0.2801457760476822
1104	20	0.3198271552386629
1104	21	0.5148854924229624
1104	22	0.6330749897088452
1104	23	1.0119291677707452
1104	24	0.6848036352196871
1104	25	0.5080758971239021
1105	1	-0.26353487347743965
1105	2	-0.19687258981121047
1105	3	-1.586425565510428
1105	4	-0.6750927130828046
1105	5	-0.5476438562185351
1105	6	-0.2965473725648735
1105	7	-0.3197214662949932
1105	8	-0.23907424999052296
1105	9	0.20550467583129262
1105	10	0.35430714208670366
1105	11	-0.2729177828198033
1105	12	-0.31326744566509035
1105	13	-0.3234235387938966
1105	14	0.47745331754329356
1105	15	0.17279331637624296
1105	16	-0.3319558452710325
1105	17	0.21141300188298828
1105	18	0.16329123805080314
1105	19	0.1250667275509585
1105	20	-0.4893414767059099
1105	21	-0.0206092600056067
1105	22	0.09787315338419944
1105	23	0.20569086431938643
1105	24	0.1071942455523498
1105	25	0.37937813035225787
1106	1	-1.782616042508213
1106	2	-1.1749192992465893
1106	3	-1.3342980673024165
1106	4	-1.4469134996006972
1106	5	-1.1027968858289157
1106	6	-0.9483766361589758
1106	7	-0.7576839845687431
1106	8	-0.9116882851340833
1106	9	-0.5153121454485543
1106	10	-2.1697530005117756
1106	11	-1.3900160983097578
1106	12	-0.9706368986076441
1106	13	-1.1106531765151004
1106	14	-0.34642003371521923
1106	15	-1.0457641308286014
1106	16	-1.5010357830695114
1106	17	-0.9093108739223581
1106	18	-0.7814596836563936
1106	19	-0.6630550091599829
1106	20	-0.38203914267477046
1106	21	-0.4667324822359973
1106	22	-0.39853148080663203
1106	23	-0.759467209155531
1106	24	-1.0170219327059655
1106	25	-1.6439024830660927
1107	1	-0.07119497333815517
1107	2	0.1196036641541447
1107	3	-0.028974085373800307
1107	4	0.21949992179779088
1107	5	-0.13372298388711812
1107	6	-0.05303807320963472
1107	7	-0.12899829932666051
1107	8	-0.12140676647861426
1107	9	0.4173911167273445
1107	10	-0.04540607399817986
1107	11	-0.05204088334568979
1107	12	-0.19292533765086262
1107	13	0.022065293079513992
1107	14	-1.2161893922312856
1107	15	-0.6277543274223021
1107	16	-0.2987332901643214
1107	17	-0.06265695063062537
1107	18	0.26263738468990283
1107	19	0.2848173852347955
1107	20	0.3338201229263902
1107	21	0.3882309993173294
1107	22	-0.34195091197375405
1107	23	0.4945771266426888
1107	24	0.3007394431656891
1107	25	-0.012199707083006217
1108	1	-0.1665998697438411
1108	2	0.10409037755220396
1108	3	-0.12121929540167287
1108	4	-0.4008873027955673
1108	5	-0.3682465823249217
1108	6	-0.27362424266970464
1108	7	-0.25215987303026566
1108	8	-0.3780274413240008
1108	9	0.23101096138702815
1108	10	0.21846669476222758
1108	11	-0.10991539208977485
1108	12	-0.10605672866476112
1108	13	-0.2261389583479314
1108	14	0.06379477839300307
1108	15	-0.19230492933994014
1108	16	-0.17357647510944219
1108	17	0.10234184660351531
1108	18	0.14270923443685035
1108	19	0.1198727850791883
1108	20	0.26002910065594875
1108	21	0.20202726503960378
1108	22	0.14635725657559817
1108	23	-0.07028370568976783
1108	24	0.031406978878003144
1108	25	-0.7619571900096034
1109	1	-0.9170885862982523
1109	2	-0.763389761191064
1109	3	-0.8634622141200516
1109	4	-0.7115376835113736
1109	5	-1.0373753934750454
1109	6	-1.2650040756386574
1109	7	-0.8957107360889509
1109	8	-1.3671295966837675
1109	9	-0.9665811403363804
1109	10	-1.1070703589244673
1109	11	-1.176332195116231
1109	12	-1.2837645688610095
1109	13	-1.2719992185673377
1109	14	-0.691318307762387
1109	15	-1.3161191675212933
1109	16	-1.4963317755493506
1109	17	-1.571176482263283
1109	18	-2.1709570071373037
1109	19	-2.2268257698408362
1109	20	-1.9885316788222624
1109	21	-1.7518410858306055
1109	22	-1.8378635520997548
1109	23	-1.3689430404127734
1109	24	-2.2745694366069813
1109	25	-1.2347087555535126
1110	1	0.15083471895512446
1110	2	0.22129236890667414
1110	3	0.09647044712276909
1110	4	0.2810951188602502
1110	5	0.2495760551548969
1110	6	0.1874178842081946
1110	7	0.15799824927299255
1110	8	0.13738409662150985
1110	9	0.4585250551352047
1110	10	0.3202966750062899
1110	11	0.31182777126403494
1110	12	0.333894901461818
1110	13	0.36809856526218
1110	14	-2.4972865885322406
1110	15	-0.5604255767121913
1110	16	-0.044731524045410254
1110	17	-0.08247322765955545
1110	18	-2.7681672912117037
1110	19	-0.4840473168476791
1110	20	-1.8745316552068658
1110	21	-0.3013863821675571
1110	22	-1.7794111600616844
1110	23	-0.4625250742509479
1110	24	0.15845489288356873
1110	25	0.10903214547571594
1111	1	-0.09882064419933201
1111	2	-1.278166678603224
1111	3	-0.28541565948460956
1111	4	0.05838751657126551
1111	5	0.061229695758058256
1111	6	0.1269753893319745
1111	7	0.08502360089828898
1111	8	0.07085847197282646
1111	9	-1.6257842784240495
1111	10	-0.2301507956077984
1111	11	-0.08806994453389194
1111	12	0.027614245763551537
1111	13	0.17835514920756879
1111	14	1.0791908206113021
1111	15	0.47772393908577726
1111	16	0.4325769068045844
1111	17	0.1522113887952159
1111	18	0.2852447355135297
1111	19	9.549829954238476E-4
1111	20	0.44060243932338494
1111	21	-0.3740776796217773
1111	22	-0.13601439893483303
1111	23	0.12464256075391944
1111	24	-0.2789341556643308
1111	25	-1.8026319430654922
1112	1	0.24075565582514358
1112	2	0.4682072316083037
1112	3	0.07031491246896593
1112	4	0.4328874676356236
1112	5	0.2175774276478677
1112	6	0.26729467074579105
1112	7	0.21305488365858594
1112	8	0.16757511662568447
1112	9	-0.20545225319234817
1112	10	0.619356196662558
1112	11	0.16765000300441812
1112	12	0.20439430077515652
1112	13	0.19850433219551178
1112	14	0.6642529353820521
1112	15	0.32482144672339786
1112	16	0.33426704134159024
1112	17	-3.7214960241252584
1112	18	-0.9171823595556177
1112	19	-1.1335813526189398
1112	20	-0.035299372811529395
1112	21	-0.3476218047638966
1112	22	-1.5151667447528965
1112	23	-1.3708507248789985
1112	24	-0.23680511418677241
1112	25	0.22854378145942178
1113	1	-0.2316567750327252
1113	2	-0.06190528502477122
1113	3	-0.07371916145725876
1113	4	-0.5120571857715106
1113	5	-0.10074096824235568
1113	6	-0.21760693693276068
1113	7	-0.22679484035079867
1113	8	-0.1884165174710759
1113	9	0.2767007667975747
1113	10	0.40414471662837315
1113	11	-0.05814532706822462
1113	12	0.027582374122694375
1113	13	-0.4053427874118114
1113	14	-1.4088680727685627
1113	15	-1.7969528479863186
1113	16	-0.46007687706148204
1113	17	-0.19859609989023852
1113	18	-0.5301042878545275
1113	19	-0.04260753459593758
1113	20	0.2858006442337384
1113	21	-1.5447809673087534
1113	22	-2.059151473214385
1113	23	-0.7070547317847513
1113	24	-2.3702317137009814
1113	25	-0.6028360833519528
1114	1	0.31746453604979763
1114	2	0.3856129720838055
1114	3	0.1452890517891241
1114	4	0.3712213742444703
1114	5	0.20845619141158522
1114	6	0.3375451406562462
1114	7	0.3560517868202382
1114	8	0.19588805222374361
1114	9	0.6693849759694928
1114	10	0.4547579241519486
1114	11	0.4398111573379566
1114	12	0.3888573895698073
1114	13	0.36701938811332013
1114	14	1.2556311391881962
1114	15	0.7081059466479473
1114	16	0.6399379183760345
1114	17	-0.2348885800118552
1114	18	0.1378755757884491
1114	19	-0.3295907510006657
1114	20	-1.42208452082793
1114	21	-0.6177199870404309
1114	22	-1.2521212032054336
1114	23	-2.8153424389048993
1114	24	-1.7812854978953467
1114	25	-1.1363903793006145
1115	1	0.47150044500390464
1115	2	0.5161032256312978
1115	3	0.3627359341659293
1115	4	0.5083578319823943
1115	5	0.5508866147375571
1115	6	0.34796965978932803
1115	7	0.569930164627074
1115	8	0.547897139633811
1115	9	0.8198630355308154
1115	10	1.0501970674136487
1115	11	0.6499797236018097
1115	12	0.48848027522475945
1115	13	0.43179120320737496
1115	14	1.2853240425571428
1115	15	0.7252053151157369
1115	16	0.6908919010199881
1115	17	0.7155404058585901
1115	18	0.8796604750046271
1115	19	0.19739556045787054
1115	20	0.818016207202362
1115	21	-0.18483637657541863
1115	22	0.8964470893496426
1115	23	0.9131335452510854
1115	24	0.9799677028082883
1115	25	1.1083486439753614
1116	1	0.6173805332699551
1116	2	0.7208130067830751
1116	3	0.5449854114953858
1116	4	0.8701685372378094
1116	5	0.7449409493446311
1116	6	0.5930721423542806
1116	7	0.5839087991760579
1116	8	0.5844621796274723
1116	9	0.8481372652929764
1116	10	0.8133388950232927
1116	11	0.7151656958544166
1116	12	0.6709927442404506
1116	13	0.07973895117107332
1116	14	1.2160470290290561
1116	15	0.8983228853295486
1116	16	0.9148384639148759
1116	17	1.1514584150950498
1116	18	1.068730750404093
1116	19	1.1709649896874694
1116	20	1.0011121756367203
1116	21	0.48408125067525276
1116	22	0.4831026358012025
1116	23	-0.40553710030703627
1116	24	-0.0049663055263309275
1116	25	-0.4735950171767698
1201	1	0.28241912711549233
1201	2	0.44687635232398293
1201	3	0.37523370361143904
1201	4	0.27396617151514135
1201	5	0.3155197629140929
1201	6	0.23534652257903588
1201	7	0.2811715394474277
1201	8	0.35138798790193704
1201	9	0.5481065833271757
1201	10	0.9242244528362349
1201	11	0.5136212651566368
1201	12	0.5229024671226767
1201	13	0.4953124086095073
1201	14	1.1194421846535647
1201	15	0.7362901165450069
1201	16	0.47196734464498746
1201	17	0.5179452088531136
1201	18	0.4358071064646154
1201	19	0.763211415541349
1201	20	0.8552855241382602
1201	21	0.7887733073717698
1201	22	0.9762371634718576
1201	23	0.5898985284372813
1201	24	0.8778519821615873
1201	25	0.9852732686087862
1202	1	0.13299881856245932
1202	2	0.48337181021225106
1202	3	0.2842677746446718
1202	4	0.2424349052077588
1202	5	0.06164208691556183
1202	6	0.04302005984992263
1202	7	0.03376457596326787
1202	8	0.1433427035935111
1202	9	0.6466341780196885
1202	10	-0.26958960776604357
1202	11	0.07573625574165277
1202	12	0.09686237346922866
1202	13	-0.013018049284532651
1202	14	0.6357055538227385
1202	15	0.45660340665943977
1202	16	0.2337022035990941
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2210	17	0.768438074556606
2210	18	0.783298307429618
2210	19	0.8891484540864596
2210	20	1.0230120731424321
2210	21	1.0406426247738736
2210	22	1.2897045105835403
2210	23	1.6218644434590104
2210	24	1.2597192538998527
2210	25	1.1229819677805182
2211	1	0.14934059318976622
2211	2	-0.4430943117487942
2211	3	0.08243246728546438
2211	4	0.37736038866025723
2211	5	0.387730855049071
2211	6	0.21442793410810168
2211	7	0.22835873566420872
2211	8	0.3842619421369662
2211	9	0.8291808303239208
2211	10	0.9649855288548039
2211	11	0.5132356665103472
2211	12	0.36450995200466707
2211	13	0.34406213048286816
2211	14	-0.755726794073432
2211	15	0.1268444009155445
2211	16	0.33708344177406957
2211	17	0.4606622928817288
2211	18	0.40305487265133166
2211	19	0.7107045698974588
2211	20	0.7581209421095707
2211	21	0.8246599424744829
2211	22	0.005452023064790978
2211	23	0.6096906004306959
2211	24	-0.11168297402031158
2211	25	0.4062606712983552
2212	1	0.17879517848152204
2212	2	-0.9742325496646558
2212	3	-0.18279767356795773
2212	4	0.3181346775709599
2212	5	0.33081159465099574
2212	6	0.3103450245451427
2212	7	0.3304728662634133
2212	8	0.256925032233589
2212	9	-2.173222521457589
2212	10	0.10481890542845917
2212	11	0.20689463456721313
2212	12	0.1611697674494934
2212	13	0.23047961374977888
2212	14	0.7569469120556036
2212	15	0.4339190972631002
2212	16	0.4509084098633238
2212	17	0.4539362998900245
2212	18	0.2957321785748874
2212	19	-0.3742347821211922
2212	20	0.5257723573208111
2212	21	0.4192182021541271
2212	22	-1.6323277593523784
2212	23	-0.21925298516634228
2212	24	0.44521141173648004
2212	25	-0.27238327265853
2213	1	0.1968203568561151
2213	2	0.4790858605933437
2213	3	0.14798840071491526
2213	4	0.30143668474642027
2213	5	0.3382437504064768
2213	6	0.21767231008495713
2213	7	0.2309270459545374
2213	8	0.15660530306300302
2213	9	0.6414038977423431
2213	10	0.6333903850150691
2213	11	0.0709735502972677
2213	12	0.021479604775677785
2213	13	0.07407009139135567
2213	14	-1.7444725259543148
2213	15	-0.5310181234500178
2213	16	-0.1425414274152388
2213	17	-2.2525692840620977
2213	18	-0.9734732956265617
2213	19	-0.5283315650696068
2213	20	0.17970231211115362
2213	21	-0.20985602357990985
2213	22	0.3445224678862573
2213	23	0.8337029622009524
2213	24	0.6463434386956237
2213	25	-0.712474882327065
2214	1	0.38367320878631617
2214	2	0.3364150924021878
2214	3	0.2539560399466012
2214	4	0.5637507725362454
2214	5	0.5901633232736643
2214	6	0.5452493227357564
2214	7	0.49339220264384664
2214	8	0.40204968232530547
2214	9	0.8265980085150701
2214	10	0.7923572176177345
2214	11	0.16161918303694023
2214	12	0.11934684143542995
2214	13	0.25321007540728857
2214	14	1.1961602972565544
2214	15	0.7917071641378257
2214	16	0.667165364326884
2214	17	0.5891650425975925
2214	18	0.5859293858348025
2214	19	-0.5436307139785228
2214	20	0.4407243082998491
2214	21	0.6602663770371748
2214	22	0.8974953228239144
2214	23	1.3908857894496052
2214	24	1.1027230481535142
2214	25	1.2342631201104624
2215	1	0.11918077646993908
2215	2	0.49623831048403133
2215	3	0.41009317556748215
2215	4	0.6595253658288911
2215	5	0.5942995825609501
2215	6	0.5022504916359125
2215	7	0.49248066429702675
2215	8	0.4893706389928951
2215	9	0.5540295154969357
2215	10	0.9757825879876378
2215	11	0.6217396578462768
2215	12	0.47808751203440636
2215	13	0.3568570142845573
2215	14	1.2841393863750596
2215	15	0.781664447143946
2215	16	0.8214864243604012
2215	17	0.9583146791206474
2215	18	0.7790413559506675
2215	19	0.1228942988682249
2215	20	-1.2289526822854242
2215	21	-1.5201640282096207
2215	22	0.1649464909613414
2215	23	0.8485451477202375
2215	24	0.9297573021190431
2215	25	0.41476419099512896
2216	1	0.31053965542103157
2216	2	0.031724204740619655
2216	3	0.15139524762406964
2216	4	0.4637402365122938
2216	5	0.5898487791172684
2216	6	0.23122803449777507
2216	7	0.4108483756599668
2216	8	0.3106175335865302
2216	9	0.6726934262665836
2216	10	-1.304703964737861
2216	11	-0.18512806132901005
2216	12	0.19472601706443982
2216	13	0.3308530723994636
2216	14	-0.12724855449220485
2216	15	-0.011545064272119987
2216	16	0.4281025860892953
2216	17	0.7008689581641505
2216	18	0.551823822374331
2216	19	-0.879029769621468
2216	20	-0.46682736783861856
2216	21	-0.4090490729297045
2216	22	-0.2533899710293781
2216	23	-4.331216865989312
2216	24	-1.7199216105813733
2216	25	-0.7173548066142956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	22.0
4	53.0
5	88.0
6	114.0
7	382.0
8	213.0
9	629.0
10	1712.0
11	3794.0
12	7295.0
13	11809.0
14	16981.0
15	22089.0
16	26213.0
17	28491.0
18	30610.0
19	32011.0
20	33332.0
21	34150.0
22	36552.0
23	38871.0
24	42073.0
25	46627.0
26	53562.0
27	61637.0
28	71892.0
29	88435.0
30	108983.0
31	134817.0
32	178449.0
33	235936.0
34	316783.0
35	435765.0
36	608840.0
37	884447.0
38	1356866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35808534020363	21.707434820685624	14.096631401384574	22.83784843772617
2	15.410346204954998	21.649017964964713	40.74194251623457	22.198693313845713
3	17.695889856246204	26.96255595712806	30.52045332295522	24.821100863670512
4	12.476422195772061	17.990526441292428	39.21226793519666	30.320783427738856
5	13.523008469677874	37.599706871700185	35.42945801983992	13.447826638782024
6	29.92778534727846	39.14202268910687	17.99014402523957	12.940047938375104
7	25.155654350361296	33.13003037926807	24.35321819006156	17.36109708030908
8	22.913426289756735	37.30483222922237	22.051773148390453	17.729968332630445
9	24.00485307407162	17.667626665718252	22.813024894233056	35.51449536597707
10	14.975329645239894	30.156184965025073	33.99136436558193	20.877121024153105
11	31.168488214943302	26.359806851834005	24.2930349998717	18.178669933350992
12	20.50344508790106	27.858016799551322	31.959352326707148	19.67918578584047
13	25.640621536699644	24.918810389603042	27.45523180737478	21.98533626632253
14	20.749395721947828	23.891338469065268	28.504747025695025	26.854518783291876
15	22.25493173741076	31.31432234216834	24.705821492131832	21.724924428289068
16	21.60993285531403	31.733824822520784	26.090151520049652	20.56609080211553
17	19.88537487036179	30.65787817433033	28.945714296682535	20.511032658625343
18	19.952936812032753	30.491094223161387	29.63552296775307	19.92044599705279
19	20.657154540598334	29.222553202149985	29.879412770132284	20.240879487119397
20	21.853837959187057	28.918836960147303	29.150585897922582	20.076739182743058
21	22.37384086487072	29.329651656926103	28.091873826622216	20.204633651580966
22	21.189551571472762	28.950835523945813	29.65001316286863	20.209599741712797
23	21.532258292625666	29.216865157374922	28.87596327561593	20.374913274383484
24	21.15543176455447	28.408761908379347	29.227056702604532	21.208749624461646
25	21.873197069811884	28.70282048300582	28.522115438087393	20.901867009094907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4400.0
1	4400.0
2	4680.0
3	4960.0
4	4960.0
5	4960.0
6	7249.0
7	9538.0
8	9538.0
9	9538.0
10	15535.5
11	21533.0
12	21533.0
13	21533.0
14	36530.5
15	51528.0
16	51528.0
17	51528.0
18	81880.5
19	112233.0
20	112233.0
21	112233.0
22	158541.5
23	204850.0
24	204850.0
25	204850.0
26	263235.0
27	321620.0
28	321620.0
29	321620.0
30	384308.5
31	446997.0
32	446997.0
33	446997.0
34	496615.0
35	546233.0
36	546233.0
37	546233.0
38	579486.0
39	612739.0
40	612739.0
41	612739.0
42	606059.0
43	599379.0
44	599379.0
45	599379.0
46	568940.0
47	538501.0
48	538501.0
49	538501.0
50	506037.5
51	473574.0
52	473574.0
53	473574.0
54	425849.0
55	378124.0
56	378124.0
57	378124.0
58	325525.0
59	272926.0
60	272926.0
61	272926.0
62	225390.0
63	177854.0
64	177854.0
65	177854.0
66	137489.0
67	97124.0
68	97124.0
69	97124.0
70	71504.5
71	45885.0
72	45885.0
73	45885.0
74	32610.5
75	19336.0
76	19336.0
77	19336.0
78	13437.5
79	7539.0
80	7539.0
81	7539.0
82	5102.5
83	2666.0
84	2666.0
85	2666.0
86	1701.5
87	737.0
88	737.0
89	737.0
90	462.5
91	188.0
92	188.0
93	188.0
94	112.5
95	37.0
96	37.0
97	37.0
98	30.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08112272559430073
2	0.021351275137742994
3	0.03324900555981549
4	0.04068256208837691
5	0.05090370231514886
6	0.016887101244231924
7	0.022139070530715538
8	0.03831917590945928
9	0.038541374610041285
10	0.02553265068505879
11	0.02349246261607862
12	0.017533497464106828
13	0.0264012456055157
14	0.041551157008833814
15	0.038642374019396734
16	0.02448225682776207
17	0.023694461434789527
18	0.02894643072127314
19	0.018422292266434825
20	0.01557410892261102
21	0.012220928532009944
22	0.020098882461735364
23	0.020321081162317362
24	0.01945248624186046
25	0.11006915631557386
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4950524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.19604822578806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09434004483211	68.97527554383707
2	9.730880377029523	15.412945432372096
3	1.770615527730688	4.206772581704662
4	0.5437029177682667	1.7223648998430934
5	0.24616339456815092	0.9747584033821487
6	0.14082750871646332	0.6691789303095971
7	0.0914318380774093	0.5068728180425667
8	0.06267237633242473	0.3970723630757958
9	0.04555845864975983	0.32472448994870556
>10	0.24879222975131465	3.4928148271829245
>50	0.015620040130591747	0.8594014089133999
>100	0.008344879044290158	1.1707983648504596
>500	6.661127357688294E-4	0.3774915729933091
>1k	3.330553488460543E-4	0.6565964122687079
>5k	5.123928443607851E-5	0.25293195127539586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6484	0.13097603405215286	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5982	0.12083569335286526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.019988187109082E-5	0.0
2	0.0	0.0	0.0	2.019988187109082E-5	0.0
3	0.0	0.0	0.0	2.019988187109082E-5	0.0
4	0.0	0.0	0.0	6.059964561327245E-5	0.0
5	0.0	0.0	0.0	6.059964561327245E-5	2.019988187109082E-5
6	0.0	0.0	0.0	6.059964561327245E-5	2.019988187109082E-5
7	0.0	0.0	0.0	8.079952748436327E-5	4.039976374218164E-5
8	0.0	0.0	0.0	8.079952748436327E-5	4.039976374218164E-5
9	0.0	0.0	0.0	1.211992912265449E-4	4.039976374218164E-5
10	0.0	0.0	0.0	1.4139917309763573E-4	4.039976374218164E-5
11	0.0	2.019988187109082E-5	0.0	1.4139917309763573E-4	4.039976374218164E-5
12	0.0	2.019988187109082E-5	0.0	1.6159905496872655E-4	4.039976374218164E-5
13	0.0	2.019988187109082E-5	0.0	1.6159905496872655E-4	4.039976374218164E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	45	6.773991E-4	14.775484	8
TTACGCT	85	5.3156782E-5	11.179028	4
GACCGCT	100	2.4053988E-5	10.448378	7
TAAGCGT	95	1.6416784E-4	10.002287	4
CGTCAGA	185	6.475602E-10	9.758298	2
GGTATCA	4270	0.0	9.575224	1
GGACCGT	110	6.854438E-5	9.498525	6
TATACTG	1050	0.0	9.049688	5
GCGTCAG	190	1.043918E-8	9.007977	1
CTTACGC	85	0.007427448	8.942951	3
GTATTAG	825	0.0	8.644018	1
TTAGACT	490	0.0	8.338642	4
GTATTAT	825	0.0	8.298258	1
CCAGTAC	540	0.0	8.270159	3
GGACTGT	485	0.0	8.225527	6
GTAGGAC	270	1.3278623E-10	8.094198	3
TAGGACT	570	0.0	8.00183	4
TCTAGAC	440	0.0	7.9902215	3
TAGAACG	155	3.890502E-5	7.9695644	4
GTGGTAT	2300	0.0	7.9374638	1
>>END_MODULE