FastQCFastQC Report
Wed 25 May 2016
SRR1295334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950524
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT168540.3404488090553646No Hit
GGTATCAACGCAGAGTACTTTTTTT140650.28411133851689235No Hit
TATCAACGCAGAGTACTTTTTTTTT98030.19801944198230328No Hit
GTGGTATCAACGCAGAGTACATGGG75830.15317570422848167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65100.13150123098080124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG508.653963E-515.2135685
GGTATCA51850.011.0904361
AGGTCGC750.002658601210.1290626
AGCGTCA3400.010.0679043
CACACCG1054.0690487E-59.9612665
GTGGTAT22500.09.6474561
CGTAGAC1002.7326925E-49.5085763
CCGATTT900.00111882769.49589914
TGGTATC23050.09.2404382
GTCGTAG1054.3999503E-49.0671581
TAGACAG6850.09.0226465
CTAAGAC5450.08.8979343
TGGACCG1401.0740961E-58.8293035
TAACCCG1651.0379463E-68.6440735
TCGTAGG1000.00288307578.5577192
TAAGACA8050.08.5044784
TACACTG10800.08.2758995
TATACTG11500.08.1855615
GTCTTAG5250.08.1604421
GTATCAA90250.08.1227671
Produced by FastQC (version 0.11.5)