FastQCFastQC Report
Wed 25 May 2016
SRR1295333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856196
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1056845.693579772825714No Hit
GCTTATGTACTCTGCGTTGATACCA625313.3687714012959837No Hit
CTTATGTACTCTGCGTTGATACCAC408202.199121213492541No Hit
GAGTACATAAGCAGTGGTATCAACG274931.4811474650306327No Hit
TATGTACTCTGCGTTGATACCACTG262971.4167146141894498No Hit
CCCATGTACTCTGCGTTGATACCAC242201.3048191031550547No Hit
GCGTTGATACCACTGCTTATGTACT217621.1723977424797811No Hit
CATAAGCAGTGGTATCAACGCAGAG186111.002641962378973No Hit
ACTCTGCGTTGATACCACTGCTTAT167420.9019521645343488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA160120.8626244211279412No Hit
CATGTACTCTGCGTTGATACCACTG159700.8603617290415452No Hit
ACGCAGAGTACATAAGCAGTGGTAT158210.8523345594969497No Hit
CTGCTTATGTACTCTGCGTTGATAC155080.8354721160911887No Hit
GTATCAACGCAGAGTACATAAGCAG146200.7876323405502437No Hit
GTACATGATAAGCAGTGGTATCAAC124580.6711575717219518No Hit
TATCAACGCAGAGTACATAAGCAGT121220.6530560350307834No Hit
ATCATGTACTCTGCGTTGATACCAC120240.6477764201625259No Hit
GTACATGGGAAGCAGTGGTATCAAC120160.6473454311936886No Hit
GGTATCAACGCAGAGTACATAAGCA115210.620677988746878No Hit
AAGCAGTGGTATCAACGCAGAGTAC98870.5326484918618508No Hit
GTATCAACGCAGAGTACTTTTTTTT90680.48852599617712783No Hit
GTGGTATCAACGCAGAGTACATAAG87380.47074770121258747No Hit
GCAGTGGTATCAACGCAGAGTACAT75070.4044292736327414No Hit
ACATAAGCAGTGGTATCAACGCAGA74290.40022713118657727No Hit
GTACTCTGCGTTGATACCACTGCTT73390.39537850528715723No Hit
GTGGTATCAACGCAGAGTACATGGG70440.37948578706128017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63770.3435520817844667No Hit
CCACTGCTTATGTACTCTGCGTTGA63000.3394038129594073No Hit
GATACCACTGCTTATGTACTCTGCG61510.3313766434148118No Hit
GGTATCAACGCAGAGTACTTTTTTT58650.31596878777887677No Hit
ATACCACTGCTTATGTACTCTGCGT56080.30212326715497717No Hit
TATCAACGCAGAGTACTTTTTTTTT54860.2955506853802077No Hit
GTTGATACCACTGCTTATGTACTCT54060.2912407956918343No Hit
GTACATGGGATAAGCAGTGGTATCA51300.276371676266946No Hit
GAGTACATGGGAAGCAGTGGTATCA51030.27491708849711993No Hit
GCTTATCCCATGTACTCTGCGTTGA47150.25401412350850877No Hit
CAGTGGTATCAACGCAGAGTACATA46580.25094332710554274No Hit
GAGTACATGATAAGCAGTGGTATCA46460.2502968436522867No Hit
GTATCAACGCAGAGTACATGGGAAG43460.23413475732088637No Hit
TACCACTGCTTATGTACTCTGCGTT40780.21969662686483538No Hit
TGATACCACTGCTTATGTACTCTGC40650.2189962697904747No Hit
GCGTTGATACCACTGCTTCCCATGT40480.21808041823169538No Hit
GCTTATCATGTACTCTGCGTTGATA40360.21743393477843934No Hit
GCAGAGTACATAAGCAGTGGTATCA38290.2062820952097731No Hit
GGTATCAACGCAGAGTACATGGGAA38100.20525849640878444No Hit
GAACAAAAAAAAAAAAAAAAAAAAA37050.19960176619279432No Hit
CCCCATGTACTCTGCGTTGATACCA36570.19701583237977022No Hit
GATAAGCAGTGGTATCAACGCAGAG36390.19604610719988624No Hit
TATCAACGCAGAGTACATGGGAAGC36070.19432215132453684No Hit
GCGTTGATACCACTGCTTATCATGT35500.19125135492157078No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA35410.19076649233162876No Hit
GGATAAGCAGTGGTATCAACGCAGA33160.1786449275830785No Hit
ACGCAGAGTACATGGGAAGCAGTGG32940.17745970791877583No Hit
ATCAACGCAGAGTACATAAGCAGTG30900.16646948921342358No Hit
ACTCTGCGTTGATACCACTGCTTCC30560.16463778609586488No Hit
CAGTGGTATCAACGCAGAGTACATG30350.16350644005266685No Hit
ACGCAGAGTACATGATAAGCAGTGG30010.16167473693510814No Hit
GTACTGGTTCACTATCGGTCAGTCA28920.15580251223469935No Hit
TATCAACGCAGAGTACATGATAAGC28780.155048281539234No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27710.14928380408103453No Hit
GGGATAAGCAGTGGTATCAACGCAG26910.1449739143926611No Hit
GAGTACATGGGATAAGCAGTGGTAT26710.14389644197056775No Hit
GTATCAACGCAGAGTACATGATAAG26150.14087951918870636No Hit
AAAAAGTACTCTGCGTTGATACCAC25860.139317184176671No Hit
ATGTACTCTGCGTTGATACCACTGC24910.13419919017172754No Hit
GTACACAAGCAGTGGTATCAACGCA23940.12897344892457477No Hit
ATGATAAGCAGTGGTATCAACGCAG23550.12687237770149273No Hit
GGTATCAACGCAGAGTACATGATAA22950.12363996043521265No Hit
GTACATAAGCAGTTGTATCAACGCA22410.1207307848955606No Hit
GTATCAACGCAGAGTACATGGGATA21890.11792935659811787No Hit
TACATAAGCAGTGGTATCAACGCAG21450.11555891726951249No Hit
CCATGTACTCTGCGTTGATACCACT21300.11475081295294247No Hit
GCTTCCCATGTACTCTGCGTTGATA21240.11442757122631447No Hit
GCGTTGATACCACTGCTTATCCCAT20620.11108740671782506No Hit
CTGCTTATCATGTACTCTGCGTTGA20530.11060254412788303No Hit
GGTATCAACGCAGAGTACATGGGAT19600.10559229736514894No Hit
ATACAGGGTGACAGCCCCGTACACA19560.10537680288073027No Hit
CATGTAAGCAGTGGTATCAACGCAG19090.10284474268881089No Hit
AAAGTACTCTGCGTTGATACCACTG18580.1000971880124728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA250.00599553219.0202371
GTAGTAC250.00599553219.0202371
GTAGTAG453.5073142E-516.9068781
CCGGCTT402.7663235E-416.6211812
GTTGTAT3950.016.35067412
AGTTGTA4200.015.60355811
GTACAAA8650.015.1722111
TGTATCA4200.015.1512814
CAGTTGT4400.015.11097910
TCTTCGT2050.014.82586211
ATCATAA902.2537279E-914.7935171
TAGACAG456.7459804E-414.7827475
CATACCG456.7459804E-414.7827475
GCTGAAC456.7487394E-414.7819513
ATACCGT456.772227E-414.7751796
GTAACTC653.3819742E-614.61163218
TTCGTTG2050.014.36255413
TATACCG400.00526784814.2547925
GACCTAT400.005287667714.24672611
GCAGTTG4750.014.1975039
Produced by FastQC (version 0.11.5)