FastQCFastQC Report
Wed 25 May 2016
SRR1295333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856196
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1114056.0017907591655195No Hit
GCTTATGTACTCTGCGTTGATACCA708673.8178619068244948No Hit
CTTATGTACTCTGCGTTGATACCAC475102.559535738682768No Hit
GAGTACATAAGCAGTGGTATCAACG314321.6933556585619192No Hit
CCCATGTACTCTGCGTTGATACCAC295201.5903492950097944No Hit
GTATCAACGCAGAGTACTTTTTTTT254511.371137530734901No Hit
GCGTTGATACCACTGCTTATGTACT252641.361063163588328No Hit
TATGTACTCTGCGTTGATACCACTG248601.339298220662042No Hit
CATAAGCAGTGGTATCAACGCAGAG230021.2392010326495693No Hit
ACTCTGCGTTGATACCACTGCTTAT193361.041700337679857No Hit
ACGCAGAGTACATAAGCAGTGGTAT188861.0174572081827566No Hit
CATGTACTCTGCGTTGATACCACTG185030.9968236112996689No Hit
CTGCTTATGTACTCTGCGTTGATAC183330.9876650957118753No Hit
GTACTTTTTTTTTTTTTTTTTTTTT177680.957226499787738No Hit
GGTATCAACGCAGAGTACTTTTTTT170050.9161209268848765No Hit
TATCAACGCAGAGTACTTTTTTTTT161280.8688737611760826No Hit
GTATCAACGCAGAGTACATAAGCAG158250.8525500539813683No Hit
ATCATGTACTCTGCGTTGATACCAC139070.7492204487026154No Hit
TATCAACGCAGAGTACATAAGCAGT137610.741354900021334No Hit
GGTATCAACGCAGAGTACATAAGCA127020.6843027352714908No Hit
GTACATGATAAGCAGTGGTATCAAC123350.6645311163260776No Hit
GTACATGGGAAGCAGTGGTATCAAC120630.649877491385608No Hit
AAGCAGTGGTATCAACGCAGAGTAC116960.6301058724401949No Hit
ACATAAGCAGTGGTATCAACGCAGA96010.5172406362259158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT95950.5169173944992879No Hit
GTGGTATCAACGCAGAGTACATAAG95510.5145469551706824No Hit
GCAGTGGTATCAACGCAGAGTACAT84500.4552320983344431No Hit
GTACTCTGCGTTGATACCACTGCTT76360.41137897075524354No Hit
GTGGTATCAACGCAGAGTACATGGG73130.3939777911384358No Hit
CCACTGCTTATGTACTCTGCGTTGA72920.3928464450952378No Hit
GATACCACTGCTTATGTACTCTGCG71060.38282595156976956No Hit
ATACCACTGCTTATGTACTCTGCGT62520.3368178791463832No Hit
GAGTACATGGGAAGCAGTGGTATCA56250.30303911871375655No Hit
CAGTGGTATCAACGCAGAGTACATA55020.29641266331788235No Hit
GAGTACTTTTTTTTTTTTTTTTTTT53240.28682315876125153No Hit
GTACATGGGATAAGCAGTGGTATCA53200.2866076642768329No Hit
GTTGATACCACTGCTTATGTACTCT53110.2861228016868908No Hit
GCTTATCCCATGTACTCTGCGTTGA52800.2844527194326461No Hit
GAGTACATGATAAGCAGTGGTATCA51900.279604093533226No Hit
TACCACTGCTTATGTACTCTGCGTT49530.26683604533141975No Hit
GCGTTGATACCACTGCTTCCCATGT47550.25616906835269554No Hit
TGATACCACTGCTTATGTACTCTGC46500.25051233813670537No Hit
GCTTATCATGTACTCTGCGTTGATA46280.2493271184724027No Hit
GTATCAACGCAGAGTACATGGGAAG45660.2459869539639133No Hit
GCAGAGTACATAAGCAGTGGTATCA45340.24426299808856392No Hit
CCCCATGTACTCTGCGTTGATACCA45150.24323939928757524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44600.2402763501268185No Hit
GATAAGCAGTGGTATCAACGCAGAG40790.21975050048594005No Hit
ACGCAGAGTACATGGGAAGCAGTGG40080.21592547338750864No Hit
GCGTTGATACCACTGCTTATCATGT40000.2154944844186713No Hit
GGATAAGCAGTGGTATCAACGCAGA39880.2148480009654153No Hit
TATCAACGCAGAGTACATGGGAAGC39860.21474025372320596No Hit
GGTATCAACGCAGAGTACATGGGAA39560.2131240450900659No Hit
CAGTGGTATCAACGCAGAGTACATG36740.1979316839385496No Hit
ACTCTGCGTTGATACCACTGCTTCC36370.1959383599576769No Hit
ACGCAGAGTACATGATAAGCAGTGG36200.19502250839889754No Hit
ATCAACGCAGAGTACATAAGCAGTG36040.19416053046122284No Hit
GCAGAGTACTTTTTTTTTTTTTTTT35710.1923827009647688No Hit
GGGATAAGCAGTGGTATCAACGCAG32560.17541251031679844No Hit
GTACTGGTTCACTATCGGTCAGTCA30660.16517652230691157No Hit
AAAAAGTACTCTGCGTTGATACCAC30640.16506877506470222No Hit
TATCAACGCAGAGTACATGATAAGC29840.1607588853763288No Hit
GAGTACATGGGATAAGCAGTGGTAT29700.16000465468086345No Hit
ATGATAAGCAGTGGTATCAACGCAG27830.14993028753429058No Hit
ATGTACTCTGCGTTGATACCACTGC27110.1460513868147545No Hit
GTATCAACGCAGAGTACATGATAAG26960.14524328249818447No Hit
ATCAACGCAGAGTACTTTTTTTTTT26080.1405024038409737No Hit
GTGGTATCAACGCAGAGTACTTTTT25980.139963667629927No Hit
CCATGTACTCTGCGTTGATACCACT25880.13942493141888035No Hit
TACATAAGCAGTGGTATCAACGCAG25630.13807809089126363No Hit
GTACACAAGCAGTGGTATCAACGCA25610.1379703436490543No Hit
GCTTCCCATGTACTCTGCGTTGATA24690.13301397050742486No Hit
GCGTTGATACCACTGCTTATCCCAT23900.1287579544401561No Hit
GGTATCAACGCAGAGTACATGATAA23810.1282730918502141No Hit
GTATCAACGCAGAGTACATGGGATA23790.12816534460800477No Hit
CTGCTTATCATGTACTCTGCGTTGA23480.12649526235376007No Hit
ATACAGGGTGACAGCCCCGTACACA22690.1222392462864913No Hit
CATGTAAGCAGTGGTATCAACGCAG22490.12116177386439794No Hit
GGTATCAACGCAGAGTACATGGGAT21360.11507405467957048No Hit
ACGCAGAGTACATGGGATAAGCAGT21290.11469693933183779No Hit
CATGATAAGCAGTGGTATCAACGCA21190.11415820312079111No Hit
GAGTACATGTAAGCAGTGGTATCAA21090.11361946690974445No Hit
CTGCGTTGATACCACTGCTTATGTA20790.11200325827660441No Hit
AAAGTACTCTGCGTTGATACCACTG20580.11087191223340638No Hit
GCTTACATGTACTCTGCGTTGATAC20230.10898633549474301No Hit
GGATACCACGTGTCCCGCCCTACTC19750.10640040168171895No Hit
ATACCACTGCTTCCCATGTACTCTG19110.10295248993102023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCT250.006034635518.99884219
GCGCCTA250.00604121218.99525816
GTCCCCA307.736474E-418.99525816
CTAGGGC350.002164662816.2921853
ATCCGGA350.002174034216.2816512
CAATGCG350.002174034216.281659
CTTATAC2250.016.072481
GTCTTAT456.674268E-414.8035991
TTCTATA801.282624E-714.255662
TCTAGTA400.00526572114.255662
TAGCCCT400.00526572114.255664
AATCCGG400.005288362514.24644511
TCGGCTT400.005288362514.24644510
CTAGATG551.9538813E-413.8236714
TGGACGG551.9543282E-413.8232985
CGTCTAT551.9646296E-413.81473310
AGATAGT500.00150256213.296686
TTACTCT1301.4551915E-1113.1590714
CGCAGAA1750.013.0337472
TATACAC3300.012.9596913
Produced by FastQC (version 0.11.5)