FastQCFastQC Report
Wed 25 May 2016
SRR1295332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186690
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA974184.4550439248361675No Hit
GCTTATGTACTCTGCGTTGATACCA577712.641938272000146No Hit
CTTATGTACTCTGCGTTGATACCAC374381.712085389332736No Hit
GAGTACATAAGCAGTGGTATCAACG260621.19184703821758No Hit
CCCATGTACTCTGCGTTGATACCAC247671.1326251091832862No Hit
TATGTACTCTGCGTTGATACCACTG245881.1244392209229475No Hit
GTACATGATAAGCAGTGGTATCAAC218520.9993186048319607No Hit
ATCATGTACTCTGCGTTGATACCAC212480.9716969483557341No Hit
GCGTTGATACCACTGCTTATGTACT196360.8979782227933544No Hit
CATGTACTCTGCGTTGATACCACTG190190.8697620604658183No Hit
ACTCTGCGTTGATACCACTGCTTAT172710.7898238890743543No Hit
CATAAGCAGTGGTATCAACGCAGAG167660.7667296233119464No Hit
CTGCTTATGTACTCTGCGTTGATAC144150.6592155266635874No Hit
ACGCAGAGTACATAAGCAGTGGTAT136930.6261975863062437No Hit
GTATCAACGCAGAGTACATAAGCAG130740.5978899615400445No Hit
GTACATGGGAAGCAGTGGTATCAAC126920.5804206357554111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA120730.552113010989212No Hit
TATCAACGCAGAGTACATAAGCAGT107090.48973562782104463No Hit
GGTATCAACGCAGAGTACATAAGCA105320.4816412019993689No Hit
AAGCAGTGGTATCAACGCAGAGTAC95150.4351325519392324No Hit
GTATCAACGCAGAGTACTTTTTTTT87540.40033109402796013No Hit
GTGGTATCAACGCAGAGTACATAAG83300.38094105703140363No Hit
GTGGTATCAACGCAGAGTACATGGG81480.37261797511307043No Hit
GCAGTGGTATCAACGCAGAGTACAT81340.3719777380424294No Hit
GAGTACATGATAAGCAGTGGTATCA78820.3604534707708912No Hit
GTACTCTGCGTTGATACCACTGCTT74400.3402402718263677No Hit
GCTTATCATGTACTCTGCGTTGATA67970.31083509779621254No Hit
ACATAAGCAGTGGTATCAACGCAGA66250.30296932807119437No Hit
GCGTTGATACCACTGCTTATCATGT60180.27521047793697323No Hit
GGTATCAACGCAGAGTACTTTTTTT56700.2592960136096109No Hit
GATACCACTGCTTATGTACTCTGCG56560.2586557765389698No Hit
CCACTGCTTATGTACTCTGCGTTGA54840.2507900068139517No Hit
GTACTGGTTCACTATCGGTCAGTCA54670.25001257608531613No Hit
GAGTACATGGGAAGCAGTGGTATCA54620.24978391998865865No Hit
GATAAGCAGTGGTATCAACGCAGAG52680.24091206343834745No Hit
GTACATGGGATAAGCAGTGGTATCA52230.2388541585684299No Hit
GTTGATACCACTGCTTATGTACTCT50820.23240605664268826No Hit
ATACCACTGCTTATGTACTCTGCGT50690.23181155079137875No Hit
ACGCAGAGTACATGATAAGCAGTGG48390.22129337034513352No Hit
TATCAACGCAGAGTACTTTTTTTTT47420.21685744206997792No Hit
GCTTATCCCATGTACTCTGCGTTGA45480.20798558551966673No Hit
TATCAACGCAGAGTACATGATAAGC44980.20569902455309166No Hit
GTATCAACGCAGAGTACATGGGAAG44900.20533317479843965No Hit
GCGTTGATACCACTGCTTCCCATGT42690.19522657532617793No Hit
GTATCAACGCAGAGTACATGATAAG42500.19435768215887939No Hit
CAGTGGTATCAACGCAGAGTACATA41100.18795531145246927No Hit
GGTATCAACGCAGAGTACATGGGAA39490.1805925851400976No Hit
ATACAGGGTGACAGCCCCGTACACA38980.17826029295419105No Hit
GGTATCAACGCAGAGTACATGATAA38460.17588226954895297No Hit
CAGTGGTATCAACGCAGAGTACATG38040.17396155833702995No Hit
GGATACCACGTGTCCCGCCCTACTC37800.17286400907307392No Hit
ATGATAAGCAGTGGTATCAACGCAG37620.1720408471251069No Hit
TGATACCACTGCTTATGTACTCTGC37170.16998294225518937No Hit
CTGCTTATCATGTACTCTGCGTTGA36560.1671933378759678No Hit
TACCACTGCTTATGTACTCTGCGTT36300.16600432617334876No Hit
TATCAACGCAGAGTACATGGGAAGC36150.16531835788337626No Hit
GAACAAAAAAAAAAAAAAAAAAAAA35110.16056231107290014No Hit
CCCCATGTACTCTGCGTTGATACCA34130.15608065157841303No Hit
ACGCAGAGTACATGGGAAGCAGTGG34090.15589772670108704No Hit
GCAGAGTACATAAGCAGTGGTATCA33220.15191911061924643No Hit
ACTCTGCGTTGATACCACTGCTTCC33080.15127887354860542No Hit
GGATAAGCAGTGGTATCAACGCAGA31130.14236128577896273No Hit
GTTAATGATAGTGTGTCGAAACACA28330.12955654436614242No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28180.12887057607616992No Hit
GTGGTATCAACGCAGAGTACATGAT27620.12630962779360586No Hit
CATGATAAGCAGTGGTATCAACGCA27120.12402306682703082No Hit
GGGATAAGCAGTGGTATCAACGCAG26810.12260539902775429No Hit
ATCAACGCAGAGTACATAAGCAGTG26190.1197700634292012No Hit
ACCCTGTATCGCGCGCCTTTCCAGA26170.1196786009905382No Hit
TACCACTGCTTATCATGTACTCTGC26080.11926702001655469No Hit
GGTATCAACGCAGAGTACATGGGGG25840.11816947075259869No Hit
GAGTACATGGGATAAGCAGTGGTAT25520.11670607173399064No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA24620.11259026199415556No Hit
ATGTACTCTGCGTTGATACCACTGC24530.11217868102017205No Hit
AAAAAGTACTCTGCGTTGATACCAC23470.10733117177103293No Hit
GCTTCCCATGTACTCTGCGTTGATA23180.1060049664104194No Hit
CCCATATTCAGACAGGATACCACGT22980.10509034202378936No Hit
GTATCAACGCAGAGTACATGGGGGT22620.10344401812785534No Hit
ATCATTAACTGAATCCATAGGTTAA22460.10271231861855132No Hit
CCATGTACTCTGCGTTGATACCACT22220.1016147693545953No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22080.1009745322839543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTG508.6933585E-515.2047435
CCGATTT456.774883E-414.77461114
TGTCAGG400.00528592114.2475986
CGTACCA400.005286723414.24727210
TCGTACC400.005286723414.2472729
CAGTTGT3450.014.04079110
CTTCGTT2250.013.930346512
GTACAAA5850.013.8159471
CGATACG551.9662E-413.81364116
AGTTGTA3550.013.64496211
ACCGACC707.2784405E-613.569148
CGTAAGG707.2818802E-613.56852114
AAATCGC1550.013.47974917
GCAGTTG3400.013.4091979
GCCTTAA500.001489667913.3120721
GCCGTCT500.001503088713.29623818
GTCTAGA655.4082342E-513.1657841
CCTACCG655.4654458E-513.15102714
GGTTTAG604.0620012E-412.6781631
GGTATAT604.0620012E-412.6781631
Produced by FastQC (version 0.11.5)