##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295332_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2186690
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.801036269430053	31.0	30.0	33.0	25.0	34.0
2	29.664803881665897	31.0	30.0	34.0	24.0	34.0
3	30.12945410643484	31.0	30.0	34.0	25.0	34.0
4	33.227896043792214	35.0	33.0	37.0	26.0	37.0
5	33.84623975049047	35.0	35.0	37.0	28.0	37.0
6	33.88322167293946	37.0	35.0	37.0	28.0	37.0
7	33.99065573995399	37.0	35.0	37.0	29.0	37.0
8	33.96222738476876	37.0	35.0	37.0	28.0	37.0
9	34.78646035789252	39.0	35.0	39.0	25.0	39.0
10	34.74692709071702	38.0	34.0	39.0	26.0	39.0
11	35.18251283903983	39.0	35.0	39.0	27.0	39.0
12	35.203486548161834	39.0	35.0	39.0	27.0	39.0
13	34.92980212101395	38.0	35.0	39.0	27.0	39.0
14	35.11766825658872	39.0	34.0	41.0	23.0	41.0
15	35.65182993474155	39.0	34.0	41.0	26.0	41.0
16	35.646706666239844	39.0	34.0	40.0	27.0	41.0
17	35.68574649355876	39.0	35.0	40.0	27.0	41.0
18	35.62374090520375	39.0	34.0	41.0	26.0	41.0
19	35.69020986056551	39.0	34.0	41.0	26.0	41.0
20	35.735411512377155	39.0	34.0	41.0	26.0	41.0
21	35.750016691895055	39.0	34.0	41.0	26.0	41.0
22	35.52205388052262	39.0	34.0	41.0	25.0	41.0
23	35.20484293612721	39.0	33.0	40.0	24.0	41.0
24	35.54541155810838	39.0	34.0	41.0	25.0	41.0
25	34.429156396196994	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6573969348069006
1101	2	0.7946745028885793
1101	3	0.6022989969167014
1101	4	0.5616591083274329
1101	5	0.5029445245478925
1101	6	0.37055944603664415
1101	7	0.42867547764465286
1101	8	0.5336505938506733
1101	9	0.5400296905829194
1101	10	0.743801602793738
1101	11	0.712261471294859
1101	12	0.6855036972143296
1101	13	0.7317486937518609
1101	14	1.1587328968836772
1101	15	0.8636250763453077
1101	16	0.8127321547686535
1101	17	0.9567628302715505
1101	18	0.8696195674918528
1101	19	0.8893420636141727
1101	20	0.9196599392398923
1101	21	1.0864344694159414
1101	22	0.8568006606684477
1101	23	-1.524070216148857
1101	24	0.3349486220855624
1101	25	0.8827842714919711
1102	1	0.513731944083009
1102	2	0.5413242081622407
1102	3	0.37949774613385756
1102	4	0.5247911339403188
1102	5	0.24040730045987857
1102	6	0.30986805531769335
1102	7	0.3518803546169167
1102	8	0.3684079667123896
1102	9	0.668582054318847
1102	10	0.9979159533108088
1102	11	0.7801972360132581
1102	12	0.6527540619276238
1102	13	0.6470568212673768
1102	14	1.3054018211346232
1102	15	0.9384123511321363
1102	16	0.7386069387050185
1102	17	1.0333496247499525
1102	18	0.8871768041238468
1102	19	1.0779212693631877
1102	20	0.973152513419933
1102	21	0.9483943495242002
1102	22	0.8173134089646794
1102	23	0.9044442721841435
1102	24	0.908602447542755
1102	25	0.7852732304938499
1103	1	-0.1957577489334632
1103	2	-1.6137459128374374
1103	3	-0.37880802523698875
1103	4	-0.04685527479598761
1103	5	-0.0430772090049274
1103	6	0.06001038532187408
1103	7	0.18915634113692903
1103	8	0.1755936719285458
1103	9	0.8030143994261962
1103	10	0.8609372584313704
1103	11	0.6410438681740516
1103	12	0.39572027002021315
1103	13	0.2765291089306885
1103	14	-0.8249836199728335
1103	15	-0.07514593284269466
1103	16	0.03643193700403202
1103	17	0.678921864088295
1103	18	0.6935056901862282
1103	19	0.6969558241494553
1103	20	0.7506183191033884
1103	21	0.7478127963494643
1103	22	0.3217706915838008
1103	23	1.0052081372683475
1103	24	0.8898065190326179
1103	25	0.8094243534290158
1104	1	0.024411230268427175
1104	2	0.25698740063727143
1104	3	-0.18814494748468036
1104	4	-0.0608819115890995
1104	5	0.1006500901243399
1104	6	-0.0025034677245940884
1104	7	0.1255240941033975
1104	8	0.06002881357037637
1104	9	-2.2697776372937497
1104	10	-0.3885587496126135
1104	11	-0.265515132442836
1104	12	0.12092875448553997
1104	13	0.33294451430552385
1104	14	-1.6311112962778367
1104	15	-0.4436454619944783
1104	16	0.06808513833690455
1104	17	0.36841558935737595
1104	18	0.42248406762627866
1104	19	0.561167485856302
1104	20	0.5774458129935525
1104	21	0.672895106749678
1104	22	0.8549352473396468
1104	23	1.161619958976587
1104	24	0.8529670478243716
1104	25	0.7515870817220431
1105	1	-0.17151007100547844
1105	2	-0.0057068618981581665
1105	3	-1.4958348116230837
1105	4	-0.4187342720621743
1105	5	-0.41462660162947884
1105	6	-0.010803405821228296
1105	7	-0.06544819599532303
1105	8	-0.05883297902076379
1105	9	0.4475145791725481
1105	10	0.6015358973752996
1105	11	-0.15203119473716953
1105	12	-0.14405226724579734
1105	13	-0.23513371200014888
1105	14	0.657227657229484
1105	15	0.18454093229738078
1105	16	-0.3543438356962625
1105	17	0.2115227310509198
1105	18	0.15511406469401123
1105	19	0.09378445063654794
1105	20	-0.4570494302215167
1105	21	-0.16618913069859786
1105	22	-0.051898558414109175
1105	23	0.25760357087506236
1105	24	0.1671987130249093
1105	25	0.4000867396244985
1106	1	-1.4939527878630905
1106	2	-0.9541929502778643
1106	3	-1.0095418492662915
1106	4	-1.1503422917021098
1106	5	-0.8697343484925426
1106	6	-0.6119490686690199
1106	7	-0.476216062880205
1106	8	-0.6428891710556783
1106	9	-0.19632066359886835
1106	10	-1.7980412984784877
1106	11	-1.0909087179231989
1106	12	-0.8104666153090818
1106	13	-0.859970045879038
1106	14	0.03290277997907509
1106	15	-0.7072621228035487
1106	16	-1.234915303235816
1106	17	-0.5523275144482653
1106	18	-0.30760415680079944
1106	19	-0.40515853899793086
1106	20	-0.10559689339663691
1106	21	-0.08385825235708211
1106	22	-0.041942202713073584
1106	23	-0.48673630910311516
1106	24	-0.763850114034561
1106	25	-1.5649705435178873
1107	1	-0.1586690999198268
1107	2	0.08085433104601236
1107	3	-0.16456548992822917
1107	4	-0.011216996193830653
1107	5	-0.3396516061757566
1107	6	-0.13493961240424568
1107	7	-0.19678343876672955
1107	8	-0.2973217951691538
1107	9	0.19091337895432048
1107	10	-0.15661103843120827
1107	11	-0.25361557371516597
1107	12	-0.3516013670821323
1107	13	-0.24418990403506058
1107	14	-1.5403241703755413
1107	15	-0.8109855270588682
1107	16	-0.4853290148197402
1107	17	-0.2626703040463738
1107	18	0.015800098518347738
1107	19	-0.09913017020360826
1107	20	0.07655440683976877
1107	21	0.22961692494788366
1107	22	-0.46493274644829796
1107	23	0.45026132683069164
1107	24	0.17474326658010142
1107	25	-0.12720458607108043
1108	1	-0.27794312614113537
1108	2	0.03674933261311608
1108	3	-0.22188763369721798
1108	4	-0.4173011459577225
1108	5	-0.4795817670932223
1108	6	-0.2887757563786906
1108	7	-0.1923795230408203
1108	8	-0.26283615132590654
1108	9	0.18875951377479794
1108	10	0.24923420193078982
1108	11	-0.10884375550419634
1108	12	-0.1931506974160513
1108	13	-0.18384119282082878
1108	14	0.046124145693504204
1108	15	-0.2381852275101508
1108	16	-0.20495709479623514
1108	17	0.0748048071720504
1108	18	0.11498733216830459
1108	19	-0.05437453010961946
1108	20	0.22574658062251274
1108	21	0.2201294162862908
1108	22	-0.04291887678012074
1108	23	-0.18320650243570213
1108	24	-0.11281425652238397
1108	25	-1.1137138522552519
1109	1	-0.9770160441110285
1109	2	-0.8436612279907258
1109	3	-0.8713640349646177
1109	4	-0.7807619170183031
1109	5	-1.0897962393745573
1109	6	-1.3473747503889513
1109	7	-0.8949262842920547
1109	8	-1.2757878557674331
1109	9	-1.0171048287781872
1109	10	-1.1099983087123135
1109	11	-1.1941684755814492
1109	12	-1.1672106241775424
1109	13	-1.2165524793120923
1109	14	-0.6904753664617758
1109	15	-1.3788370445839178
1109	16	-1.4288066817417828
1109	17	-1.5787355748154113
1109	18	-2.1604080791247497
1109	19	-2.2052884413678484
1109	20	-1.9734230510685862
1109	21	-1.7671804573160586
1109	22	-1.7579908165884461
1109	23	-1.3764203060727738
1109	24	-2.1942877159554897
1109	25	-1.3698775145883957
1110	1	-0.07556044385052019
1110	2	0.11586775644666147
1110	3	-0.035625271300844474
1110	4	0.16045917742402338
1110	5	0.12844211039882936
1110	6	0.03850165049317411
1110	7	0.05889051504625087
1110	8	-0.046800797348673484
1110	9	0.22179602431710066
1110	10	0.14699162819386657
1110	11	0.19813989814283417
1110	12	0.1484064308272437
1110	13	0.11471228678757939
1110	14	-2.7859510053210883
1110	15	-0.7899833202505135
1110	16	-0.32426725797675005
1110	17	-0.2718125405127765
1110	18	-3.018581858678928
1110	19	-0.6875643110104548
1110	20	-1.99871133941366
1110	21	-0.4220748314922176
1110	22	-1.9123473820844836
1110	23	-0.5992940623694594
1110	24	0.013618801549171167
1110	25	-0.05299821829827778
1111	1	-0.28611810650627234
1111	2	-1.2782974775359506
1111	3	-0.4003128486710601
1111	4	-0.02002810302550273
1111	5	-0.05826856261236202
1111	6	0.1374553111429222
1111	7	0.08539308422747638
1111	8	-0.05672635004908244
1111	9	-1.6019024145121392
1111	10	-0.388435901716079
1111	11	-0.1787547203430293
1111	12	-0.06960380247232223
1111	13	-0.07520115507087866
1111	14	0.8101400515639838
1111	15	0.2865589499352552
1111	16	0.3176467194607753
1111	17	-0.029164463908081473
1111	18	0.12514075358384247
1111	19	-0.10753926907032962
1111	20	0.36715007889982587
1111	21	-0.4253581181382984
1111	22	-0.10082748649827522
1111	23	0.031286356365306744
1111	24	-0.2133540880778142
1111	25	-1.8955881833680337
1112	1	0.13160896475918804
1112	2	0.2103644184935085
1112	3	-0.03958844202719902
1112	4	0.21904993671718387
1112	5	0.11231825771829307
1112	6	0.05572634274735577
1112	7	0.017290788321332684
1112	8	-0.12552246537044454
1112	9	-0.3016099748063752
1112	10	0.4526208695767764
1112	11	0.024291309619314916
1112	12	-0.06304757883820145
1112	13	-0.010345312697182862
1112	14	0.48502929194859234
1112	15	0.04696106340683315
1112	16	0.18312504655417428
1112	17	-3.766666838279427
1112	18	-0.9917855701910554
1112	19	-0.9483520940485448
1112	20	-0.03514772081980766
1112	21	-0.39618352950368063
1112	22	-1.5964052383576828
1112	23	-1.2875109424433333
1112	24	-0.350931525129603
1112	25	0.14226166286738362
1113	1	-0.42161540738130654
1113	2	-0.331176246793774
1113	3	-0.2533404662598855
1113	4	-0.5932035869003371
1113	5	-0.3731243197583467
1113	6	-0.3481450259497336
1113	7	-0.579288795286736
1113	8	-0.432490878498335
1113	9	0.028134687661506064
1113	10	0.15149336249670142
1113	11	-0.4068348837621656
1113	12	-0.36536499368147446
1113	13	-0.622700016369734
1113	14	-1.8411052257345517
1113	15	-1.9009646823065935
1113	16	-0.9149918170409634
1113	17	-0.7448007041659608
1113	18	-0.9696099590054175
1113	19	-0.44917252408272645
1113	20	0.0076573793623424535
1113	21	-1.8173723509391948
1113	22	-2.2845072975802267
1113	23	-0.856556540416193
1113	24	-2.7897687502950177
1113	25	-0.8013724569166669
1114	1	0.09251523106414794
1114	2	0.2189063294724214
1114	3	0.02920107783737791
1114	4	0.3084485049845256
1114	5	0.03045205610469992
1114	6	0.18950963002835408
1114	7	0.0033643613281242324
1114	8	0.08132633030650283
1114	9	0.3864865455162061
1114	10	0.3177688997280512
1114	11	0.2054392961508995
1114	12	0.22699739977976208
1114	13	0.12283275129291127
1114	14	0.9732687506617097
1114	15	0.44265294729120086
1114	16	0.30896367716412954
1114	17	-0.2653041421795308
1114	18	-0.024582968443922937
1114	19	-0.31646248879002314
1114	20	-1.5917400830109258
1114	21	-0.7348531455329095
1114	22	-1.4048195617239116
1114	23	-3.104699535557124
1114	24	-2.2310511773128567
1114	25	-1.4541861000293679
1115	1	0.21795275944572268
1115	2	0.339984849491227
1115	3	0.23520772465201034
1115	4	0.4292855053180702
1115	5	0.30372213619600075
1115	6	0.1813353339919317
1115	7	0.39124778889834033
1115	8	0.30966731913069623
1115	9	0.5194883418439815
1115	10	0.7423677399513267
1115	11	0.3483787635216302
1115	12	0.22305914201677268
1115	13	0.16915469984640197
1115	14	0.9722122527373287
1115	15	0.3738720791866683
1115	16	0.4674982434132602
1115	17	0.5478649095442591
1115	18	0.7905568196135135
1115	19	0.0791973904099521
1115	20	0.5919840621259311
1115	21	-0.32521566617862163
1115	22	0.7949509744819352
1115	23	0.7706775124373806
1115	24	0.8131662145156611
1115	25	0.8200231339794968
1116	1	0.18482384062057378
1116	2	0.388310852120874
1116	3	0.12094328630115214
1116	4	0.4359912418067111
1116	5	0.3776735661092303
1116	6	0.15298701548699967
1116	7	0.09526416932335025
1116	8	0.06438851723554961
1116	9	0.27642245139352895
1116	10	0.23163873878782226
1116	11	0.18168183341993682
1116	12	0.061411161658014635
1116	13	-0.2957643216086012
1116	14	0.6448800086150186
1116	15	0.470508202958662
1116	16	0.39998126964770364
1116	17	0.5134129196893298
1116	18	0.47327169511424216
1116	19	0.7119677829553339
1116	20	0.4599869548740898
1116	21	0.06992787043093074
1116	22	0.08358494164338026
1116	23	-0.9128749240758651
1116	24	-0.39468714687269113
1116	25	-0.8997306416225825
1201	1	0.581664344195822
1201	2	0.7985453968073877
1201	3	0.6565168994474639
1201	4	0.5569933084078258
1201	5	0.5024980642823635
1201	6	0.3165664057029858
1201	7	0.3599882367518674
1201	8	0.3901647564969437
1201	9	0.6317987639974305
1201	10	1.0183926188054642
1201	11	0.636400400952617
1201	12	0.6694856204697714
1201	13	0.5305707422519177
1201	14	1.147192207298417
1201	15	0.8298877677989154
1201	16	0.5539892088000471
1201	17	0.744006017014982
1201	18	0.6268481844342944
1201	19	0.8798233203980033
1201	20	0.8319962329868105
1201	21	0.8500037469654416
1201	22	0.9869588793318869
1201	23	0.5852685738404588
1201	24	1.016766709112126
1201	25	1.2462151681200027
1202	1	0.46392496497303526
1202	2	0.7845409337844202
1202	3	0.5789675599184179
1202	4	0.3676179626596294
1202	5	0.20657158424261723
1202	6	0.1950088978171607
1202	7	0.3633029441688649
1202	8	0.327051788385738
1202	9	0.9049585844426389
1202	10	0.08154248499058525
1202	11	0.2971846256743049
1202	12	0.43815990464028687
1202	13	0.4046225462343713
1202	14	1.129431721281371
1202	15	0.8114716103150172
1202	16	0.619370312474409
1202	17	-1.301552418437275
1202	18	0.21053744566087573
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2210	19	0.6752130702225116
2210	20	0.8651044549651701
2210	21	0.8442644840969749
2210	22	0.9811608353704457
2210	23	1.4071616127040727
2210	24	1.0460564880112457
2210	25	0.9643376285086589
2211	1	0.1058911362624606
2211	2	-0.3856339749748656
2211	3	-0.05726217159216773
2211	4	0.34075900817141047
2211	5	0.25745729526254024
2211	6	0.3546062566160657
2211	7	0.23292204595920651
2211	8	0.3797750846989345
2211	9	0.7393528181500528
2211	10	0.9296556866938062
2211	11	0.4433840317239728
2211	12	0.3861119877475119
2211	13	0.27879763760661547
2211	14	-0.7509991366762137
2211	15	0.16358137970930642
2211	16	0.2891150370432456
2211	17	0.4755284167318763
2211	18	0.458752238549188
2211	19	0.7050040095221703
2211	20	0.7293730097907911
2211	21	0.7683869837163328
2211	22	-0.09327414254715904
2211	23	0.6205534338319083
2211	24	0.06336320417614161
2211	25	0.5130625441716106
2212	1	-0.36476400615203985
2212	2	-1.5436469586777335
2212	3	-0.7593422369526337
2212	4	-0.3539399286615392
2212	5	-0.20437227713979667
2212	6	-0.31808551272372654
2212	7	-0.3115874417820734
2212	8	-0.33338662494100646
2212	9	-2.5193314634327137
2212	10	-0.40338929312472516
2212	11	-0.3329707631225034
2212	12	-0.3916988271212105
2212	13	-0.3239136775154705
2212	14	0.10624665161181213
2212	15	-0.25047491952975065
2212	16	-0.2557030603472441
2212	17	-0.21477809213168086
2212	18	-0.3694958969278801
2212	19	-0.9268347439793914
2212	20	-0.16029835952792837
2212	21	-0.1762960610061839
2212	22	-2.121265597737768
2212	23	-0.7738192001355557
2212	24	-0.1776487479017277
2212	25	-1.06934009167869
2213	1	0.11953029931129677
2213	2	0.5287777679573402
2213	3	0.2057740404224795
2213	4	0.37580116092730265
2213	5	0.39708594118021523
2213	6	0.31642287054506113
2213	7	0.08499755886793281
2213	8	0.08607171988151663
2213	9	0.6383179887881028
2213	10	0.6609996822690789
2213	11	0.11274297335936012
2213	12	0.11246781282641649
2213	13	0.2659073301790329
2213	14	-1.8089326146852898
2213	15	-0.34866819262767734
2213	16	-0.09362616605961449
2213	17	-1.9545028238385171
2213	18	-0.8301227926437562
2213	19	-0.41623207028766274
2213	20	0.2759544321259355
2213	21	-0.21966222863891005
2213	22	0.5036875467823165
2213	23	0.8860924317409768
2213	24	0.7058694763295037
2213	25	-0.9355529767462016
2214	1	0.3402151738845056
2214	2	0.2586277918873634
2214	3	0.10975858342117917
2214	4	0.4378661362000855
2214	5	0.5100266112462748
2214	6	0.3736267112678462
2214	7	0.42205281455482435
2214	8	0.11663591624581926
2214	9	0.740110615121182
2214	10	0.6111155758856128
2214	11	0.05065872131979887
2214	12	0.04378963555274851
2214	13	0.0535161256381258
2214	14	0.994476703943846
2214	15	0.5601310935811838
2214	16	0.5647988472469976
2214	17	0.48162745773078797
2214	18	0.5369266884864103
2214	19	-0.8753737274089133
2214	20	0.09601944213926572
2214	21	0.5336823610081538
2214	22	0.7891324722122945
2214	23	1.3077002468387917
2214	24	0.9853985526842806
2214	25	0.9066068091503467
2215	1	-0.24920350330417307
2215	2	0.20891404351221965
2215	3	0.22963547832089048
2215	4	0.2353189115962735
2215	5	0.3323016893777577
2215	6	0.10659527736692809
2215	7	0.003930305620023944
2215	8	0.03283164290701279
2215	9	0.18746059806593252
2215	10	0.48374973616808603
2215	11	0.14419370609299875
2215	12	-0.005079032877830514
2215	13	-0.08825909538868615
2215	14	0.8272584305497261
2215	15	0.246601824621429
2215	16	0.2682326375381052
2215	17	0.5452450759574745
2215	18	0.4194021888505617
2215	19	-0.28499780511939576
2215	20	-1.6138292731855728
2215	21	-2.0215028958807864
2215	22	-0.15917782885127707
2215	23	0.5171310317183142
2215	24	0.6504027166236455
2215	25	-0.16697660638621414
2216	1	-0.049074781753301266
2216	2	-0.32112223400233475
2216	3	-0.11156713432884047
2216	4	0.19936240829731844
2216	5	0.05894147324315213
2216	6	-0.112757586997418
2216	7	-0.014671197851150453
2216	8	-0.16629852106885323
2216	9	0.15340249265059214
2216	10	-1.790308706471201
2216	11	-0.5175366649153119
2216	12	-0.28403226668974924
2216	13	-0.1120986667165269
2216	14	-0.6311411766564277
2216	15	-0.5948515442962332
2216	16	-0.15578201642760803
2216	17	0.14210177408252633
2216	18	0.1939975957739435
2216	19	-1.1946008910931951
2216	20	-0.9425149687942636
2216	21	-0.917043455058618
2216	22	-0.7318486525957013
2216	23	-4.5692755430533865
2216	24	-2.0685071450006873
2216	25	-1.1561845033640097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	33.0
5	36.0
6	43.0
7	177.0
8	112.0
9	235.0
10	651.0
11	1502.0
12	3242.0
13	5668.0
14	9234.0
15	14328.0
16	19765.0
17	23237.0
18	23400.0
19	22018.0
20	21478.0
21	20115.0
22	18676.0
23	18225.0
24	18453.0
25	18723.0
26	20378.0
27	22826.0
28	25918.0
29	31901.0
30	39615.0
31	49609.0
32	68305.0
33	93985.0
34	129522.0
35	183724.0
36	267408.0
37	401080.0
38	613059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.13200189486259	18.886196109123947	12.117325863730088	19.86447613228338
2	12.094303797757856	24.807395077602298	38.63314199773032	24.465159126909526
3	20.86312603559662	32.06788115673912	30.117642860867967	16.951349946796295
4	12.64118028940844	21.814793786534626	31.332267956952446	34.21175796710448
5	9.177368670535676	46.631819953769536	32.29836399807465	11.892447377620138
6	28.102010161497184	32.45266414550298	24.24863650418151	15.196689188818327
7	21.68741823637578	40.18090916575945	19.58740817316049	18.54426442470428
8	20.776217374536273	38.85451345506583	24.218478006762645	16.15079116363525
9	27.21047510077403	23.555434981020948	17.47806669408568	31.75602322411934
10	14.801449327734975	30.461086940909382	28.078826522093742	26.658637209261904
11	26.500171304387997	31.093964916678367	19.635828369927825	22.770035409005807
12	25.819254288206444	29.669480722338832	23.504919527948527	21.0063454615062
13	28.043424662108542	22.942769669338205	29.95625605235917	19.057549616194088
14	28.033074064364765	27.66304275719074	22.693560732394804	21.610322446049683
15	31.99912893181604	24.554332420480716	23.189079440137395	20.25745920756585
16	22.651934459824165	26.468204232116882	30.580017748177152	20.2998435598818
17	20.433255403227687	34.44041045730228	25.742916647416664	19.383417492053372
18	20.90247493976532	24.693405817752982	36.29565518910282	18.108464053378885
19	22.195377762083783	35.2728887774859	22.72985289469628	19.801880565734038
20	20.61458074147798	30.777016890359032	29.009036122535605	19.599366245627387
21	21.38559810026134	33.33726672905215	24.467413645955965	20.80972152473054
22	19.95764388386374	30.955551235577307	20.61100755869135	28.475797321867603
23	23.67154752386302	26.39953380891654	23.703977810182653	26.224940857037783
24	19.078053923508115	32.34687786592994	20.394832235179496	28.180235975382452
25	23.273397136283357	32.02468007731065	22.23949087040396	22.46243191600204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13910.0
1	13910.0
2	12318.5
3	10727.0
4	10727.0
5	10727.0
6	14473.0
7	18219.0
8	18219.0
9	18219.0
10	17419.5
11	16620.0
12	16620.0
13	16620.0
14	18293.5
15	19967.0
16	19967.0
17	19967.0
18	24392.5
19	28818.0
20	28818.0
21	28818.0
22	36227.5
23	43637.0
24	43637.0
25	43637.0
26	54451.0
27	65265.0
28	65265.0
29	65265.0
30	74026.0
31	82787.0
32	82787.0
33	82787.0
34	94843.5
35	106900.0
36	106900.0
37	106900.0
38	160569.0
39	214238.0
40	214238.0
41	214238.0
42	415157.5
43	616077.0
44	616077.0
45	616077.0
46	496769.0
47	377461.0
48	377461.0
49	377461.0
50	301664.5
51	225868.0
52	225868.0
53	225868.0
54	176311.5
55	126755.0
56	126755.0
57	126755.0
58	105762.5
59	84770.0
60	84770.0
61	84770.0
62	76100.5
63	67431.0
64	67431.0
65	67431.0
66	50766.5
67	34102.0
68	34102.0
69	34102.0
70	27122.5
71	20143.0
72	20143.0
73	20143.0
74	14619.0
75	9095.0
76	9095.0
77	9095.0
78	6001.5
79	2908.0
80	2908.0
81	2908.0
82	1816.0
83	724.0
84	724.0
85	724.0
86	464.5
87	205.0
88	205.0
89	205.0
90	130.0
91	55.0
92	55.0
93	55.0
94	30.5
95	6.0
96	6.0
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0839167874733044
2	0.021905254059788996
3	0.03438987693728878
4	0.04211845300431245
5	0.05112750321261816
6	0.018201025293937412
7	0.02332292185906553
8	0.03928311740575939
9	0.03928311740575939
10	0.02620398867695009
11	0.02391742771037504
12	0.018429681390594917
13	0.02556375160630908
14	0.04248430275896446
15	0.039694698379742904
16	0.02515217063232557
17	0.024832052097005064
18	0.030045411100796178
19	0.019801617970539948
20	0.016188851643351366
21	0.012987666290146293
22	0.02126501698914798
23	0.02112782333115348
24	0.02035039260251796
25	0.10316963081186634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2186690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.09609703133176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48122976704335	12.904344281416202
2	8.423297879816255	2.8801103575429954
3	3.701019941376778	1.8981898809801352
4	2.1745456537400143	1.4870497398160016
5	1.4265532788590651	1.2194246637869524
6	1.0951969880694095	1.123415638587415
7	0.8455233313355737	1.0118604240337485
8	0.6960902511550288	0.9520341181048373
9	0.5598138671813292	0.8613568973535367
>10	4.699587889793901	16.28320289295312
>50	0.5192018931166639	6.115338125315151
>100	0.3038070929924164	9.991627957024486
>500	0.03019200923004465	3.6339435149699186
>1k	0.03369643887281769	12.122423739593124
>5k	0.005121858708668288	5.807968161856781
>10k+	0.005121858708668288	21.707709606665624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	97418	4.4550439248361675	No Hit
GCTTATGTACTCTGCGTTGATACCA	57771	2.641938272000146	No Hit
CTTATGTACTCTGCGTTGATACCAC	37438	1.712085389332736	No Hit
GAGTACATAAGCAGTGGTATCAACG	26062	1.19184703821758	No Hit
CCCATGTACTCTGCGTTGATACCAC	24767	1.1326251091832862	No Hit
TATGTACTCTGCGTTGATACCACTG	24588	1.1244392209229475	No Hit
GTACATGATAAGCAGTGGTATCAAC	21852	0.9993186048319607	No Hit
ATCATGTACTCTGCGTTGATACCAC	21248	0.9716969483557341	No Hit
GCGTTGATACCACTGCTTATGTACT	19636	0.8979782227933544	No Hit
CATGTACTCTGCGTTGATACCACTG	19019	0.8697620604658183	No Hit
ACTCTGCGTTGATACCACTGCTTAT	17271	0.7898238890743543	No Hit
CATAAGCAGTGGTATCAACGCAGAG	16766	0.7667296233119464	No Hit
CTGCTTATGTACTCTGCGTTGATAC	14415	0.6592155266635874	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	13693	0.6261975863062437	No Hit
GTATCAACGCAGAGTACATAAGCAG	13074	0.5978899615400445	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12692	0.5804206357554111	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	12073	0.552113010989212	No Hit
TATCAACGCAGAGTACATAAGCAGT	10709	0.48973562782104463	No Hit
GGTATCAACGCAGAGTACATAAGCA	10532	0.4816412019993689	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9515	0.4351325519392324	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8754	0.40033109402796013	No Hit
GTGGTATCAACGCAGAGTACATAAG	8330	0.38094105703140363	No Hit
GTGGTATCAACGCAGAGTACATGGG	8148	0.37261797511307043	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8134	0.3719777380424294	No Hit
GAGTACATGATAAGCAGTGGTATCA	7882	0.3604534707708912	No Hit
GTACTCTGCGTTGATACCACTGCTT	7440	0.3402402718263677	No Hit
GCTTATCATGTACTCTGCGTTGATA	6797	0.31083509779621254	No Hit
ACATAAGCAGTGGTATCAACGCAGA	6625	0.30296932807119437	No Hit
GCGTTGATACCACTGCTTATCATGT	6018	0.27521047793697323	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5670	0.2592960136096109	No Hit
GATACCACTGCTTATGTACTCTGCG	5656	0.2586557765389698	No Hit
CCACTGCTTATGTACTCTGCGTTGA	5484	0.2507900068139517	No Hit
GTACTGGTTCACTATCGGTCAGTCA	5467	0.25001257608531613	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5462	0.24978391998865865	No Hit
GATAAGCAGTGGTATCAACGCAGAG	5268	0.24091206343834745	No Hit
GTACATGGGATAAGCAGTGGTATCA	5223	0.2388541585684299	No Hit
GTTGATACCACTGCTTATGTACTCT	5082	0.23240605664268826	No Hit
ATACCACTGCTTATGTACTCTGCGT	5069	0.23181155079137875	No Hit
ACGCAGAGTACATGATAAGCAGTGG	4839	0.22129337034513352	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4742	0.21685744206997792	No Hit
GCTTATCCCATGTACTCTGCGTTGA	4548	0.20798558551966673	No Hit
TATCAACGCAGAGTACATGATAAGC	4498	0.20569902455309166	No Hit
GTATCAACGCAGAGTACATGGGAAG	4490	0.20533317479843965	No Hit
GCGTTGATACCACTGCTTCCCATGT	4269	0.19522657532617793	No Hit
GTATCAACGCAGAGTACATGATAAG	4250	0.19435768215887939	No Hit
CAGTGGTATCAACGCAGAGTACATA	4110	0.18795531145246927	No Hit
GGTATCAACGCAGAGTACATGGGAA	3949	0.1805925851400976	No Hit
ATACAGGGTGACAGCCCCGTACACA	3898	0.17826029295419105	No Hit
GGTATCAACGCAGAGTACATGATAA	3846	0.17588226954895297	No Hit
CAGTGGTATCAACGCAGAGTACATG	3804	0.17396155833702995	No Hit
GGATACCACGTGTCCCGCCCTACTC	3780	0.17286400907307392	No Hit
ATGATAAGCAGTGGTATCAACGCAG	3762	0.1720408471251069	No Hit
TGATACCACTGCTTATGTACTCTGC	3717	0.16998294225518937	No Hit
CTGCTTATCATGTACTCTGCGTTGA	3656	0.1671933378759678	No Hit
TACCACTGCTTATGTACTCTGCGTT	3630	0.16600432617334876	No Hit
TATCAACGCAGAGTACATGGGAAGC	3615	0.16531835788337626	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3511	0.16056231107290014	No Hit
CCCCATGTACTCTGCGTTGATACCA	3413	0.15608065157841303	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3409	0.15589772670108704	No Hit
GCAGAGTACATAAGCAGTGGTATCA	3322	0.15191911061924643	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3308	0.15127887354860542	No Hit
GGATAAGCAGTGGTATCAACGCAGA	3113	0.14236128577896273	No Hit
GTTAATGATAGTGTGTCGAAACACA	2833	0.12955654436614242	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2818	0.12887057607616992	No Hit
GTGGTATCAACGCAGAGTACATGAT	2762	0.12630962779360586	No Hit
CATGATAAGCAGTGGTATCAACGCA	2712	0.12402306682703082	No Hit
GGGATAAGCAGTGGTATCAACGCAG	2681	0.12260539902775429	No Hit
ATCAACGCAGAGTACATAAGCAGTG	2619	0.1197700634292012	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2617	0.1196786009905382	No Hit
TACCACTGCTTATCATGTACTCTGC	2608	0.11926702001655469	No Hit
GGTATCAACGCAGAGTACATGGGGG	2584	0.11816947075259869	No Hit
GAGTACATGGGATAAGCAGTGGTAT	2552	0.11670607173399064	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2462	0.11259026199415556	No Hit
ATGTACTCTGCGTTGATACCACTGC	2453	0.11217868102017205	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2347	0.10733117177103293	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2318	0.1060049664104194	No Hit
CCCATATTCAGACAGGATACCACGT	2298	0.10509034202378936	No Hit
GTATCAACGCAGAGTACATGGGGGT	2262	0.10344401812785534	No Hit
ATCATTAACTGAATCCATAGGTTAA	2246	0.10271231861855132	No Hit
CCATGTACTCTGCGTTGATACCACT	2222	0.1016147693545953	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2208	0.1009745322839543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8292487732600413E-4	0.0
2	0.0	0.0	0.0	1.8292487732600413E-4	0.0
3	4.5731219331501034E-5	0.0	0.0	1.8292487732600413E-4	0.0
4	4.5731219331501034E-5	0.0	0.0	1.8292487732600413E-4	0.0
5	4.5731219331501034E-5	0.0	0.0	1.8292487732600413E-4	0.0
6	9.146243866300207E-5	0.0	0.0	1.8292487732600413E-4	0.0
7	9.146243866300207E-5	0.0	0.0	1.8292487732600413E-4	0.0
8	9.146243866300207E-5	0.0	0.0	1.8292487732600413E-4	0.0
9	9.146243866300207E-5	0.0	0.0	1.8292487732600413E-4	0.0
10	1.371936579945031E-4	0.0	0.0	2.743873159890062E-4	0.0
11	1.371936579945031E-4	0.0	0.0	2.743873159890062E-4	0.0
12	1.371936579945031E-4	0.0	0.0	2.743873159890062E-4	0.0
13	1.371936579945031E-4	0.0	0.0	3.2011853532050726E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTTG	50	8.6933585E-5	15.204743	5
CCGATTT	45	6.774883E-4	14.774611	14
TGTCAGG	40	0.005285921	14.247598	6
CGTACCA	40	0.0052867234	14.247272	10
TCGTACC	40	0.0052867234	14.247272	9
CAGTTGT	345	0.0	14.040791	10
CTTCGTT	225	0.0	13.9303465	12
GTACAAA	585	0.0	13.815947	1
CGATACG	55	1.9662E-4	13.813641	16
AGTTGTA	355	0.0	13.644962	11
ACCGACC	70	7.2784405E-6	13.56914	8
CGTAAGG	70	7.2818802E-6	13.568521	14
AAATCGC	155	0.0	13.479749	17
GCAGTTG	340	0.0	13.409197	9
GCCTTAA	50	0.0014896679	13.312072	1
GCCGTCT	50	0.0015030887	13.296238	18
GTCTAGA	65	5.4082342E-5	13.165784	1
CCTACCG	65	5.4654458E-5	13.151027	14
GGTTTAG	60	4.0620012E-4	12.678163	1
GGTATAT	60	4.0620012E-4	12.678163	1
>>END_MODULE