FastQCFastQC Report
Wed 25 May 2016
SRR1295332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186690
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1023424.680224448824479No Hit
GCTTATGTACTCTGCGTTGATACCA649882.9719804819155895No Hit
CTTATGTACTCTGCGTTGATACCAC434151.9854208872771173No Hit
GAGTACATAAGCAGTGGTATCAACG296281.3549245663537128No Hit
CCCATGTACTCTGCGTTGATACCAC290301.3275772971934752No Hit
GTATCAACGCAGAGTACTTTTTTTT261981.1980664840466642No Hit
TATGTACTCTGCGTTGATACCACTG247301.1309330540680207No Hit
ATCATGTACTCTGCGTTGATACCAC242201.107610132208955No Hit
GCGTTGATACCACTGCTTATGTACT224361.0260256369215572No Hit
CATGTACTCTGCGTTGATACCACTG219811.0052179321257242No Hit
GTACATGATAAGCAGTGGTATCAAC216470.989943704869003No Hit
CATAAGCAGTGGTATCAACGCAGAG204500.9352034353291963No Hit
ACTCTGCGTTGATACCACTGCTTAT198090.9058897237377039No Hit
GGTATCAACGCAGAGTACTTTTTTT176990.8093968509482368No Hit
TATCAACGCAGAGTACTTTTTTTTT168170.769061915497853No Hit
ACGCAGAGTACATAAGCAGTGGTAT167300.7650832994160123No Hit
CTGCTTATGTACTCTGCGTTGATAC166890.7632083194234208No Hit
GTACTTTTTTTTTTTTTTTTTTTTT162130.7414402590216262No Hit
GTATCAACGCAGAGTACATAAGCAG143100.6544137486337799No Hit
TATCAACGCAGAGTACATAAGCAGT124900.571182929450448No Hit
GTACATGGGAAGCAGTGGTATCAAC124780.5706341548184699No Hit
GGTATCAACGCAGAGTACATAAGCA116900.5345979539852471No Hit
AAGCAGTGGTATCAACGCAGAGTAC113400.5185920272192218No Hit
ACGCAGAGTACTTTTTTTTTTTTTT98270.4494006923706607No Hit
GCAGTGGTATCAACGCAGAGTACAT93500.4275869007495347No Hit
GAGTACATGATAAGCAGTGGTATCA90470.4137303412920899No Hit
GTGGTATCAACGCAGAGTACATAAG89170.40778528277899473No Hit
GTGGTATCAACGCAGAGTACATGGG88100.4028920423105241No Hit
ACATAAGCAGTGGTATCAACGCAGA81610.3732124809643799No Hit
GTACTCTGCGTTGATACCACTGCTT77490.35437121859980153No Hit
GCTTATCATGTACTCTGCGTTGATA76090.3479688478933914No Hit
GCGTTGATACCACTGCTTATCATGT66880.30585039488907895No Hit
GATACCACTGCTTATGTACTCTGCG66590.3045241895284654No Hit
CCACTGCTTATGTACTCTGCGTTGA65260.2984419373573758No Hit
GAGTACATGGGAAGCAGTGGTATCA61410.28083541791474786No Hit
GATAAGCAGTGGTATCAACGCAGAG61240.28005798718611236No Hit
ACGCAGAGTACATGATAAGCAGTGG59830.2736098852603707No Hit
GTACTGGTTCACTATCGGTCAGTCA58990.2697684628365246No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56370.2577868833716713No Hit
ATACCACTGCTTATGTACTCTGCGT53920.2465827346354536No Hit
GTTGATACCACTGCTTATGTACTCT52480.23999743905171741No Hit
TATCAACGCAGAGTACATGATAAGC52240.23889988978776142No Hit
GTACATGGGATAAGCAGTGGTATCA51290.2345554239512688No Hit
GCTTATCCCATGTACTCTGCGTTGA50560.23121704494006926No Hit
GCGTTGATACCACTGCTTCCCATGT49030.22422016838234957No Hit
CAGTGGTATCAACGCAGAGTACATA48840.22335127521505108No Hit
GTATCAACGCAGAGTACATGGGAAG48140.220150089861846No Hit
GTATCAACGCAGAGTACATGATAAG46590.2130617508654633No Hit
ATGATAAGCAGTGGTATCAACGCAG46210.21132396453086627No Hit
ATACAGGGTGACAGCCCCGTACACA45890.20986056551225823No Hit
CAGTGGTATCAACGCAGAGTACATG44000.20121736505860455No Hit
TGATACCACTGCTTATGTACTCTGC43780.20021127823331153No Hit
GGTATCAACGCAGAGTACATGGGAA42670.19513511288751492No Hit
TACCACTGCTTATGTACTCTGCGTT42620.1949064567908574No Hit
GGATACCACGTGTCCCGCCCTACTC42020.19216258363096736No Hit
CCCCATGTACTCTGCGTTGATACCA41720.19079064705102233No Hit
ACGCAGAGTACATGGGAAGCAGTGG41190.18836689242645277No Hit
CTGCTTATCATGTACTCTGCGTTGA40840.18676629974985023No Hit
GGTATCAACGCAGAGTACATGATAA40230.18397669537062866No Hit
TATCAACGCAGAGTACATGGGAAGC40010.18297060854533564No Hit
ACTCTGCGTTGATACCACTGCTTCC39390.18013527294678258No Hit
GCAGAGTACATAAGCAGTGGTATCA38800.17743713100622402No Hit
GGATAAGCAGTGGTATCAACGCAGA37460.17130914761580288No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36990.16915978030722234No Hit
CATGATAAGCAGTGGTATCAACGCA34190.15635503889440203No Hit
GGGATAAGCAGTGGTATCAACGCAG33000.1509130237939534No Hit
GTTAATGATAGTGTGTCGAAACACA32170.14711733258943885No Hit
ATCAACGCAGAGTACATAAGCAGTG31240.14286432919160924No Hit
ACCCTGTATCGCGCGCCTTTCCAGA30350.13879425067110565No Hit
TACCACTGCTTATCATGTACTCTGC29830.1364162272658676No Hit
GGTATCAACGCAGAGTACATGGGGG28590.13074555606876145No Hit
GTGGTATCAACGCAGAGTACATGAT28060.12832180144419192No Hit
GAGTACATGGGATAAGCAGTGGTAT27550.12598950925828536No Hit
GCTTCCCATGTACTCTGCGTTGATA27500.12576085316162786No Hit
AAAAAGTACTCTGCGTTGATACCAC27370.12516634731031834No Hit
CCCATATTCAGACAGGATACCACGT26990.12342856097572129No Hit
CCATGTACTCTGCGTTGATACCACT26460.12100480635115175No Hit
ATCAACGCAGAGTACTTTTTTTTTT25910.11848958928791918No Hit
ATGTACTCTGCGTTGATACCACTGC25880.11835239562992467No Hit
GTGGTATCAACGCAGAGTACTTTTT25850.11821520197193018No Hit
GTATCAACGCAGAGTACATGGGGGT25160.1150597478380566No Hit
ATCATTAACTGAATCCATAGGTTAA24820.11350488638078558No Hit
ACTTAGATGTTTCAGTTCCCCCGGT24280.11103540053688452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23740.10856591469298345No Hit
GCGTTGATACCACTGCTTATCCCAT23700.10838298981565746No Hit
TACATAAGCAGTGGTATCAACGCAG23380.10691959079704941No Hit
TGATAAGCAGTGGTATCAACGCAGA23370.10687385957771792No Hit
GTATCAACGCAGAGTACATGGGATA23210.1061421600684139No Hit
GTACACAAGCAGTGGTATCAACGCA22750.10403852397916485No Hit
GTATTTAGCCTTGGAGGATGGTCCC22580.10326109325052935No Hit
GATACCACTGCTTATCATGTACTCT22300.1019806191092473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCC250.00600387119.0158424
GACCGCT359.948236E-518.99844619
CGCTAAA401.29031905E-518.99192814
TCCCGAT453.5528737E-516.88171412
CCTAGTT402.744256E-416.6388652
TCCGATC402.7707266E-416.61793714
TAGTACA350.002158527516.2992954
CGCTAGG350.002176748816.27879518
TTCGCTA551.1332813E-515.53885112
CCGATTT508.748171E-515.19354314
CGGACCG508.748171E-515.19354317
AATTCAC456.722442E-414.7897625
GGTGTCC456.785712E-414.77158
ACTTAGC456.785712E-414.77158
AAATCGC1850.014.37227117
GACCCTT400.005294915314.24394711
CCTATTG400.005294915314.2439478
TCGCATA602.5795185E-514.24394611
GGTGACG551.9678053E-413.81231211
AATCGCC2050.013.43331518
Produced by FastQC (version 0.11.5)