FastQCFastQC Report
Wed 25 May 2016
SRR1295331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934751
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA918781.5481357179096478No Hit
GCTTATGTACTCTGCGTTGATACCA539540.9091198602940544No Hit
CTTATGTACTCTGCGTTGATACCAC335480.565280666366626No Hit
GAGTACATAAGCAGTGGTATCAACG237200.3996797843751153No Hit
GTATCAACGCAGAGTACTTTTTTTT221940.37396682691489497No Hit
TATGTACTCTGCGTTGATACCACTG219080.36914775362942776No Hit
GCGTTGATACCACTGCTTATGTACT184900.31155477289611644No Hit
CCCATGTACTCTGCGTTGATACCAC151160.25470318805287706No Hit
CATAAGCAGTGGTATCAACGCAGAG146950.2476093773774165No Hit
GTGGTATCAACGCAGAGTACATGGG140150.2361514408944874No Hit
GGTATCAACGCAGAGTACTTTTTTT136990.2308268704112439No Hit
CTGCTTATGTACTCTGCGTTGATAC124950.21053958287382235No Hit
TATCAACGCAGAGTACTTTTTTTTT124510.20979818698375047No Hit
GTATCAACGCAGAGTACATAAGCAG121890.20538351145650424No Hit
ACTCTGCGTTGATACCACTGCTTAT120040.20226627873688383No Hit
ACGCAGAGTACATAAGCAGTGGTAT118230.19921644564363356No Hit
TATCAACGCAGAGTACATAAGCAGT97340.16401699077181164No Hit
CATGTACTCTGCGTTGATACCACTG91580.154311444574507No Hit
GGTATCAACGCAGAGTACATAAGCA90490.15247480475591985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA89710.1511605120417015No Hit
GTACATGGGAAGCAGTGGTATCAAC75180.12667759776273682No Hit
GTACATGATAAGCAGTGGTATCAAC74940.12627320000451578No Hit
ATCATGTACTCTGCGTTGATACCAC71560.12057793157623631No Hit
GTGGTATCAACGCAGAGTACATAAG69910.11779769698846675No Hit
AAGCAGTGGTATCAACGCAGAGTAC67730.11412441735129242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4050.011.027223
TGGACCG3150.010.5580665
CCGCTAG951.6300939E-410.0093841
TACATAA208300.09.999162
GTACATA210850.09.9711691
TATACCG2400.09.8981875
GTACAAA11900.09.8285421
ACATAAG213400.09.6713853
TAGACCG800.0045248389.4984156
CGCCCTA8100.09.49737516
CATAAGC218450.09.3956884
CCCCGTA9200.09.1882215
GTCCTAA4250.09.1733061
GTATTGG4800.09.112711
TGTCCCG7700.09.00468711
TGTAGGA5950.08.9430022
CTAGAAC4300.08.8392363
GTCCCGC8600.08.83551212
GCTTATG129650.08.7938211
GTACTGG18650.08.7696161
Produced by FastQC (version 0.11.5)