##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295331_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5934751
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.036796489018663	31.0	30.0	33.0	26.0	34.0
2	29.90571162968758	31.0	30.0	34.0	25.0	34.0
3	30.315254001389444	31.0	30.0	34.0	26.0	34.0
4	33.66451549525835	35.0	35.0	37.0	28.0	37.0
5	34.04931900260011	35.0	35.0	37.0	29.0	37.0
6	34.162784420104565	37.0	35.0	37.0	30.0	37.0
7	34.169350660204614	37.0	35.0	37.0	30.0	37.0
8	34.08647035065161	37.0	35.0	37.0	29.0	37.0
9	35.03263793207162	39.0	35.0	39.0	27.0	39.0
10	34.96793884023104	38.0	35.0	39.0	27.0	39.0
11	35.354656833959844	39.0	35.0	39.0	28.0	39.0
12	35.35813549717587	39.0	35.0	39.0	27.0	39.0
13	35.19980130590146	39.0	35.0	39.0	27.0	39.0
14	35.38337008578793	39.0	34.0	41.0	25.0	41.0
15	35.97889970446949	39.0	35.0	41.0	27.0	41.0
16	36.051497358524394	39.0	35.0	41.0	27.0	41.0
17	35.95428974189482	39.0	35.0	41.0	27.0	41.0
18	35.92900965853496	39.0	35.0	41.0	27.0	41.0
19	35.98629327498323	39.0	35.0	41.0	27.0	41.0
20	35.9937183548223	39.0	35.0	41.0	27.0	41.0
21	35.998938792882804	39.0	35.0	41.0	27.0	41.0
22	35.76286081758106	39.0	34.0	41.0	26.0	41.0
23	35.41785106064265	39.0	34.0	41.0	25.0	41.0
24	35.760081593987685	39.0	34.0	41.0	26.0	41.0
25	34.54000108850396	38.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4363064184323626
1101	2	0.6532844877727833
1101	3	0.4262096611419999
1101	4	0.3153382076525517
1101	5	0.27325632651921694
1101	6	0.23916403404052744
1101	7	0.24087522402555095
1101	8	0.23493255586135575
1101	9	0.26582755507530464
1101	10	0.6041858327726999
1101	11	0.4262220332335289
1101	12	0.48920291405060823
1101	13	0.48621580069061565
1101	14	1.0889434764493018
1101	15	0.7694160792241505
1101	16	0.5345032891131325
1101	17	0.8296292669635079
1101	18	0.8520046119723617
1101	19	0.781878763700476
1101	20	0.8236505917670058
1101	21	0.8603853613657151
1101	22	0.5919615654949126
1101	23	-1.5759669741916866
1101	24	0.09211786808731404
1101	25	0.6086629469751372
1102	1	0.44306571050736565
1102	2	0.573816982387676
1102	3	0.4689369949010427
1102	4	0.6295096401197426
1102	5	0.4309977143807018
1102	6	0.38459547494902324
1102	7	0.36202871689025073
1102	8	0.36082546365280876
1102	9	0.6112363054129304
1102	10	1.1452789770456988
1102	11	0.7898452229969593
1102	12	0.627191821692179
1102	13	0.6896336980345126
1102	14	1.2276559843142394
1102	15	0.9283510119304736
1102	16	0.7277676243756872
1102	17	1.071453505593368
1102	18	1.0030974543983149
1102	19	1.0288270380461313
1102	20	1.1054462746256846
1102	21	1.0250509903030647
1102	22	0.9460789933625762
1102	23	1.026177649053757
1102	24	1.0336276393445019
1102	25	0.9708427354011775
1103	1	-0.33083598770571854
1103	2	-1.5567796644522431
1103	3	-0.38457657071151274
1103	4	0.07031960399675796
1103	5	-0.026856399425533084
1103	6	0.03857383737719289
1103	7	0.027666913930360693
1103	8	0.12415673868323296
1103	9	0.8132164899041783
1103	10	0.7355342232430857
1103	11	0.5581833259911448
1103	12	0.3453925894907144
1103	13	0.1755633280753628
1103	14	-0.966746620880329
1103	15	-0.049563959677243474
1103	16	0.1372199966181995
1103	17	0.5228255743492554
1103	18	0.7013939530551525
1103	19	0.7087225623323192
1103	20	0.6308024146207174
1103	21	0.7776570807905543
1103	22	0.3288759065573146
1103	23	1.0340004955833209
1103	24	0.9264423676186553
1103	25	0.8135201693893634
1104	1	-0.005437572576138194
1104	2	0.009175830449386524
1104	3	-0.37900201022203817
1104	4	-0.18502889529070643
1104	5	-0.1711947135521612
1104	6	-0.024442637087574326
1104	7	0.03390026810364333
1104	8	-0.04977658235295479
1104	9	-2.292411938037361
1104	10	-0.616166073417105
1104	11	-0.49913332627640017
1104	12	0.014957979277603783
1104	13	0.10574968827110354
1104	14	-1.6839806170005431
1104	15	-0.49941695596539404
1104	16	-0.13815976765430094
1104	17	0.2034157788527935
1104	18	0.3353784842810228
1104	19	0.36345106464123234
1104	20	0.44553777758994784
1104	21	0.5414712482255055
1104	22	0.7038517113065268
1104	23	1.1522826322156163
1104	24	0.787500929230383
1104	25	0.6938080400431872
1105	1	-0.1114427291849509
1105	2	-0.03653949210514895
1105	3	-1.3083646429996492
1105	4	-0.37388487539545423
1105	5	-0.35716118183012213
1105	6	-0.10757694578066435
1105	7	-0.007029708698880199
1105	8	-0.0323990567675736
1105	9	0.4508224630227673
1105	10	0.5026458831364735
1105	11	-0.13575431982326336
1105	12	-0.11313927028142245
1105	13	-0.20679382212988173
1105	14	0.6400182418459366
1105	15	0.29907727858299893
1105	16	-0.10581119541210882
1105	17	0.46014404776816065
1105	18	0.23558855651626942
1105	19	0.35480235041281105
1105	20	-0.3410839675127164
1105	21	0.08946152744307767
1105	22	0.1709094512741629
1105	23	0.42477002149465193
1105	24	0.3076915171749661
1105	25	0.5395280178389825
1106	1	-1.7011144131688845
1106	2	-1.1167641020779193
1106	3	-1.1365972976895549
1106	4	-1.336007545807881
1106	5	-1.0124705068672668
1106	6	-0.8086337974318667
1106	7	-0.6601995946662029
1106	8	-0.7725281492686804
1106	9	-0.3741277438945403
1106	10	-1.9168976964106506
1106	11	-1.2018909617821336
1106	12	-0.8586715569211236
1106	13	-1.0065694168938535
1106	14	-0.19879717711742728
1106	15	-0.9579094150554326
1106	16	-1.4655972838319826
1106	17	-0.8025113392610521
1106	18	-0.6382885829415059
1106	19	-0.6283784814870401
1106	20	-0.37299414902590655
1106	21	-0.2648410735186957
1106	22	-0.16290706874781336
1106	23	-0.6036287902300685
1106	24	-0.8870647857907485
1106	25	-1.6682752685867897
1107	1	-0.06943851982417826
1107	2	0.14275703466347167
1107	3	0.003477971241583333
1107	4	0.0986303568252822
1107	5	-0.1237843393933602
1107	6	-0.04506620626148816
1107	7	-0.09909995453299558
1107	8	-0.16221196978032282
1107	9	0.46382365989420293
1107	10	-0.019335836567570652
1107	11	-0.04366088944329505
1107	12	-0.14423548091691885
1107	13	0.04830198182460066
1107	14	-1.1584615432106702
1107	15	-0.4426390877154205
1107	16	-0.23358794607380418
1107	17	4.6225779590969296E-4
1107	18	0.2733132042153841
1107	19	0.11682946626022783
1107	20	0.20694102633386535
1107	21	0.3618896758688095
1107	22	-0.2795399703380852
1107	23	0.517278184694284
1107	24	0.4142937483231037
1107	25	0.03813215542733417
1108	1	-0.022633388829920165
1108	2	0.20818156670524246
1108	3	-0.02561286407290808
1108	4	-0.256470050100738
1108	5	-0.26224854010695253
1108	6	-0.1440377804027193
1108	7	-0.04623357492286573
1108	8	-0.10230561390648774
1108	9	0.37521430344935425
1108	10	0.43529528397506567
1108	11	0.14640462873425975
1108	12	0.14098050779846716
1108	13	-0.04434849153908971
1108	14	0.2799232174133408
1108	15	0.08295356471874271
1108	16	0.04724763808513899
1108	17	0.33007288509303123
1108	18	0.3400308032436925
1108	19	0.3613166361563458
1108	20	0.5133180514396471
1108	21	0.37930737168452566
1108	22	0.37142703057119775
1108	23	0.1756453723963105
1108	24	0.26571445994178333
1108	25	-0.6191260498236417
1109	1	-0.8258506747379712
1109	2	-0.7450906269021829
1109	3	-0.8152445373086508
1109	4	-0.5959858277824281
1109	5	-1.0215551837929553
1109	6	-1.1091620143122896
1109	7	-0.8001103504499554
1109	8	-1.2275189867956726
1109	9	-0.8861356391255129
1109	10	-1.1905738530894183
1109	11	-1.194145877829989
1109	12	-1.0914389203570636
1109	13	-1.1604797171968784
1109	14	-0.5889949544027573
1109	15	-1.2890141000567397
1109	16	-1.418594488067761
1109	17	-1.2995651818272052
1109	18	-1.9845131948365022
1109	19	-2.1952138627409994
1109	20	-1.889020982477028
1109	21	-1.5886903177975071
1109	22	-1.7154731100500058
1109	23	-1.2082643942079159
1109	24	-2.143863985493212
1109	25	-1.262663671304857
1110	1	0.0011414144578481
1110	2	0.2133289096125317
1110	3	0.023018195555341237
1110	4	0.19624440330296267
1110	5	0.17511881854137812
1110	6	0.11946785420582984
1110	7	0.07611524851954954
1110	8	0.014426900151669031
1110	9	0.30592755185240605
1110	10	0.22573257756672405
1110	11	0.1696814860424709
1110	12	0.21981217994341762
1110	13	0.2068149203463392
1110	14	-2.5967754772532317
1110	15	-0.7873197942050112
1110	16	-0.2401807319396596
1110	17	-0.24195628195309382
1110	18	-2.951235279371396
1110	19	-0.572028678527964
1110	20	-1.968614143389857
1110	21	-0.352728314834998
1110	22	-1.9051324656335495
1110	23	-0.5452039032953024
1110	24	0.09222188289663791
1110	25	0.12451052005310004
1111	1	-0.14093091689583304
1111	2	-1.4205945939564728
1111	3	-0.3726094528101349
1111	4	0.04438485648473289
1111	5	-0.043743681612006924
1111	6	0.052377447307662806
1111	7	-0.013485261013578054
1111	8	-0.0106571263123314
1111	9	-1.706113391329474
1111	10	-0.20200260950548454
1111	11	-0.06267820710222338
1111	12	-0.028648142834271084
1111	13	0.04515637189748389
1111	14	0.8922599704048935
1111	15	0.36905789880622564
1111	16	0.3151376175146865
1111	17	-0.07824199020503642
1111	18	0.1629942516313605
1111	19	-0.016939827530116247
1111	20	0.340838082691981
1111	21	-0.44698524472411094
1111	22	-0.2243588465490589
1111	23	0.026871519952088363
1111	24	-0.5087802256052925
1111	25	-1.9763718256235663
1112	1	0.1597863308476093
1112	2	0.3117588789265717
1112	3	-0.006637987765383713
1112	4	0.28319271348201625
1112	5	0.19778687139366014
1112	6	0.09067540114946127
1112	7	0.12012681720472074
1112	8	-0.010689105413746347
1112	9	-0.36936909307642196
1112	10	0.39218106535171415
1112	11	-0.00394233721182502
1112	12	-0.003103544289409399
1112	13	-0.024421177448921583
1112	14	0.45435644246003903
1112	15	0.1601305643611184
1112	16	0.13450058138548826
1112	17	-3.8193148671541124
1112	18	-1.0509512140677444
1112	19	-1.2430037630144426
1112	20	-0.1227096079131158
1112	21	-0.5994913482855608
1112	22	-1.753439336337678
1112	23	-1.523110628700202
1112	24	-0.3985274903555265
1112	25	-0.010764987536077797
1113	1	-0.35190024457635616
1113	2	-0.17344386520816713
1113	3	-0.16916913051565885
1113	4	-0.4451738497218116
1113	5	-0.26975255786189223
1113	6	-0.3192926996331025
1113	7	-0.43807527727967255
1113	8	-0.2935175269967232
1113	9	0.10463811213652718
1113	10	0.25489730376683184
1113	11	-0.15607000407754867
1113	12	-0.21341573372774292
1113	13	-0.5508562886076689
1113	14	-1.7412885815901475
1113	15	-2.0378533176022984
1113	16	-0.667547268169784
1113	17	-0.448710356932871
1113	18	-0.8000963806618628
1113	19	-0.29278290376983307
1113	20	0.10620378443559275
1113	21	-1.7539472570049455
1113	22	-2.11916037166597
1113	23	-0.8260892937744586
1113	24	-2.4658530235099363
1113	25	-0.7438438562549408
1114	1	0.007880375790808358
1114	2	0.1305938944550853
1114	3	-0.06799979436650361
1114	4	0.23536919610397433
1114	5	-0.019060869105238964
1114	6	0.035215011031802135
1114	7	-0.00922854524009864
1114	8	-0.014076002739976445
1114	9	0.3528363188031918
1114	10	0.16845419915046023
1114	11	0.03746786850169315
1114	12	0.07490874388205526
1114	13	-0.07055973153565986
1114	14	0.8354903860026113
1114	15	0.25475193158691667
1114	16	0.17918921840922764
1114	17	-0.6024392931110896
1114	18	-0.3286375362763394
1114	19	-0.6749971479882007
1114	20	-1.690179533892568
1114	21	-0.8299829472767399
1114	22	-1.590078844002015
1114	23	-3.1068645310779104
1114	24	-2.18690180298443
1114	25	-1.6110434678146603
1115	1	0.19688652701560372
1115	2	0.3086933343901883
1115	3	0.21934468364304394
1115	4	0.30280686829100034
1115	5	0.27161307901289433
1115	6	0.18530635216530555
1115	7	0.21913351254356428
1115	8	0.2762591549921112
1115	9	0.45443153246905155
1115	10	0.6940735799056483
1115	11	0.3449853612833991
1115	12	0.19829485738613783
1115	13	0.14826427223648153
1115	14	0.9181934616938179
1115	15	0.3788996630015049
1115	16	0.37836440965730134
1115	17	0.34174758112612125
1115	18	0.5574115339616199
1115	19	-0.049189011437952956
1115	20	0.541033490975515
1115	21	-0.6204417069466075
1115	22	0.5982547145885846
1115	23	0.7040245488905867
1115	24	0.6912250117081982
1115	25	0.8009672572012434
1116	1	0.30791670519284153
1116	2	0.475802104275882
1116	3	0.2891379390889064
1116	4	0.45654272939908935
1116	5	0.40923486738311965
1116	6	0.21165461373524863
1116	7	0.13357422132187224
1116	8	0.20206516395536767
1116	9	0.37449849369961896
1116	10	0.34458691424503485
1116	11	0.24532981855701053
1116	12	0.2524473310561248
1116	13	-0.26252943593468103
1116	14	0.7911381414696308
1116	15	0.43535608731390596
1116	16	0.37958918952401177
1116	17	0.5718995073143489
1116	18	0.48899905558890566
1116	19	0.7461865575394881
1116	20	0.5338602866981361
1116	21	0.015327136427984556
1116	22	0.049314512932802756
1116	23	-0.8806835134802142
1116	24	-0.4485110038178206
1116	25	-1.0723578522073751
1201	1	0.5538425731773344
1201	2	0.872958012528084
1201	3	0.7214931821417103
1201	4	0.6623161844284837
1201	5	0.6014553561246601
1201	6	0.4700223249907012
1201	7	0.5529133294317745
1201	8	0.5358950109209317
1201	9	0.8855472542728435
1201	10	1.1472789144936328
1201	11	0.7631821931517138
1201	12	0.7954372994891514
1201	13	0.8499546037801196
1201	14	1.3828456294632545
1201	15	1.0029737991051206
1201	16	0.7598066222915065
1201	17	0.9138367719818419
1201	18	0.9103745653643784
1201	19	1.1050118719769273
1201	20	1.1361613322870099
1201	21	1.1251623581361159
1201	22	1.185653687476922
1201	23	0.7358267827521061
1201	24	1.0735734233584893
1201	25	1.2359373385981343
1202	1	0.44379703309511953
1202	2	0.8155491808311268
1202	3	0.5836307668972864
1202	4	0.5851705963709506
1202	5	0.3964031850435461
1202	6	0.3988378692137857
1202	7	0.3459257687059818
1202	8	0.40085605918352485
1202	9	0.9081572504052744
1202	10	0.05661694441041476
1202	11	0.4569999889575982
1202	12	0.4567024844424026
1202	13	0.4995801338196699
1202	14	1.2076206179710027
1202	15	0.9001714625353685
1202	16	0.8016423024816035
1202	17	-1.352088203325522
1202	18	0.21122336323927726
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2210	21	0.9503255894976164
2210	22	1.1190371356705384
2210	23	1.4159701550797337
2210	24	1.1249153982945685
2210	25	0.9949156781601332
2211	1	0.0387039438950616
2211	2	-0.643329143016711
2211	3	-0.1819281973363438
2211	4	0.06117582954583156
2211	5	0.058899356548188564
2211	6	0.0014668102525945415
2211	7	0.08344793201806766
2211	8	0.11328526913535342
2211	9	0.6235340061746655
2211	10	0.634966370670405
2211	11	0.2815762959151442
2211	12	0.1154145218861089
2211	13	0.046537373904882884
2211	14	-1.0860030796871527
2211	15	-0.20796032383158547
2211	16	-0.017426909709087113
2211	17	0.12763391965422954
2211	18	0.12639798796672608
2211	19	0.37741056041838306
2211	20	0.5034421330689867
2211	21	0.526309539861046
2211	22	-0.19857124589804442
2211	23	0.3962088724035553
2211	24	-0.2309468244426327
2211	25	0.24098011205536096
2212	1	-0.14650584141848455
2212	2	-1.291260699838901
2212	3	-0.41315954517006404
2212	4	-0.009749823141589786
2212	5	0.015953957085805825
2212	6	0.07239333619433097
2212	7	0.002722276398998247
2212	8	-2.928373810888729E-4
2212	9	-2.263773616680517
2212	10	-0.11912655668700012
2212	11	-0.05580993542177026
2212	12	-0.1450450219570243
2212	13	-0.10469873316482392
2212	14	0.444677119926304
2212	15	0.16142219492700605
2212	16	0.2879567343000602
2212	17	0.2391466861644247
2212	18	-0.0056092775091372005
2212	19	-0.600834741083581
2212	20	0.2782353043450314
2212	21	0.21449740306076848
2212	22	-1.8370242509376595
2212	23	-0.43586370001963815
2212	24	0.11535352451603131
2212	25	-0.4991926114096401
2213	1	-0.02157045524917578
2213	2	0.2779267916197874
2213	3	-0.04105681293093966
2213	4	0.08791557515912984
2213	5	0.06998106398090442
2213	6	0.008993207056704478
2213	7	-0.02598233159708485
2213	8	0.006740400849807315
2213	9	0.4163084359313558
2213	10	0.37792534787281085
2213	11	-0.21872294720763108
2213	12	-0.10576261414666277
2213	13	-0.10622131253974487
2213	14	-1.9804830465399732
2213	15	-0.67737697726362
2213	16	-0.3547090031519886
2213	17	-2.353138271383628
2213	18	-1.1602354917259206
2213	19	-0.7234925509983654
2213	20	-0.010962035508015333
2213	21	-0.3630898163565348
2213	22	0.155557459021324
2213	23	0.6221492853744479
2213	24	0.37907843857268375
2213	25	-1.2729840371438002
2214	1	0.13104399905169117
2214	2	0.12180406363012608
2214	3	0.09086134840144311
2214	4	0.3408314286788823
2214	5	0.28579000991981474
2214	6	0.14731617143994669
2214	7	0.16311993672567127
2214	8	0.08046962781260447
2214	9	0.5147601054492412
2214	10	0.5390732118698622
2214	11	-0.1286778290701136
2214	12	-0.14566201862444217
2214	13	-0.06488157382695192
2214	14	0.7498244071013076
2214	15	0.4073885196877143
2214	16	0.34905409964044054
2214	17	0.23031299070818534
2214	18	0.15334883104944197
2214	19	-1.0643557443583234
2214	20	0.010750055850415663
2214	21	0.3791090696996804
2214	22	0.6154582995294788
2214	23	1.0548912831509227
2214	24	0.7673450252182192
2214	25	0.8011505215918646
2215	1	-0.24563939896280473
2215	2	0.15964834855062193
2215	3	0.11924844282134828
2215	4	0.24271642462762344
2215	5	0.1886338337843796
2215	6	0.06369558692769317
2215	7	0.07468011771783978
2215	8	0.09836365063297592
2215	9	0.19432202231348583
2215	10	0.5470082300713983
2215	11	0.19249510844845474
2215	12	0.01411130747234779
2215	13	-0.031327368737201766
2215	14	0.844725353058962
2215	15	0.2504737122975129
2215	16	0.23365119640562426
2215	17	0.46282418482340404
2215	18	0.3208938057573292
2215	19	-0.5225348274647388
2215	20	-1.7295341763595218
2215	21	-1.9486575992266495
2215	22	-0.32034859536899774
2215	23	0.401471083230895
2215	24	0.5097111249615267
2215	25	-0.2545632245696865
2216	1	0.0029199636950352215
2216	2	-0.23781209619644628
2216	3	-0.13067269334580445
2216	4	0.11860872677267764
2216	5	0.13404415808163606
2216	6	-0.03880960024359581
2216	7	-0.01189953636859542
2216	8	-0.03964120354127232
2216	9	0.1800804283138291
2216	10	-1.716025434542182
2216	11	-0.5934583930646511
2216	12	-0.2923525362858399
2216	13	-0.06311911339199838
2216	14	-0.6619817116793456
2216	15	-0.5240327674496186
2216	16	-0.18692735127502402
2216	17	0.19265753031731947
2216	18	0.04043620215731636
2216	19	-1.4295521417876813
2216	20	-1.0086952259654467
2216	21	-0.9126882797197453
2216	22	-0.8194979034315679
2216	23	-4.468402258827602
2216	24	-2.155231479603721
2216	25	-1.274784763245357
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	18.0
4	32.0
5	78.0
6	100.0
7	341.0
8	207.0
9	545.0
10	1725.0
11	4205.0
12	8988.0
13	16231.0
14	24940.0
15	33674.0
16	41909.0
17	46878.0
18	47865.0
19	47547.0
20	46050.0
21	44934.0
22	44323.0
23	45097.0
24	47033.0
25	50881.0
26	56540.0
27	64670.0
28	75932.0
29	94344.0
30	116045.0
31	146545.0
32	196032.0
33	263741.0
34	359676.0
35	501228.0
36	711280.0
37	1062235.0
38	1732882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49398005651307	19.519251070328348	12.931950928472318	22.054817944686267
2	15.641195164482328	21.454150605096974	39.1318401726282	23.772814057792495
3	19.12666736333267	28.107911316665035	29.222183643405884	23.543237676596405
4	12.643408272054952	18.30097132382377	35.83878974315754	33.21683066096374
5	12.241041026326565	40.73074672597369	32.984376951785286	14.04383529591446
6	29.446334512088416	36.3854461720569	19.757753022273214	14.41046629358147
7	25.48395686326721	35.3062091211371	20.760943021964408	18.44889099363128
8	23.95793775244487	35.85510618971263	21.09802382747507	19.08893223036743
9	26.594931669262984	18.6030722284157	19.354678310035023	35.44731779228629
10	15.926676585408932	28.457391766275425	29.938394363709538	25.677537284606107
11	31.544723120118917	27.14265490543715	21.326761740724887	19.985860233719045
12	23.34568377258115	27.50114089612082	27.15538364780722	21.99779168349081
13	28.07544613175518	22.91139891177306	26.835251676707106	22.177903279764656
14	24.447484151104323	23.57272287483207	25.20376429181858	26.776028682245023
15	26.805015146438045	27.631091022237925	23.168353012802836	22.395540818521198
16	23.353271312180347	28.653910876880634	25.69290950957493	22.299908301364088
17	20.785203908542705	30.35998413770334	25.964499937390105	22.89031201636385
18	20.885964584068596	27.181691959667383	30.315061091183903	21.617282365080122
19	22.48640142314666	29.38192515436325	26.34809896302478	21.783574459465314
20	23.39378631699554	27.699643733022718	27.622562934853917	21.284007015127823
21	24.291966881583953	28.329244900046696	25.50769511280419	21.871093105565162
22	23.254385786037243	27.114915624482304	25.509528426358212	24.121170163122237
23	24.37478417951815	26.066328027583225	25.64396450070116	23.914923292197464
24	22.147453300882162	27.255631178336852	25.309841623583253	25.287073897197732
25	23.545124866721174	28.37516136416119	25.493218108681305	22.586495660436334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11420.0
1	11420.0
2	11377.5
3	11335.0
4	11335.0
5	11335.0
6	16270.5
7	21206.0
8	21206.0
9	21206.0
10	24543.5
11	27881.0
12	27881.0
13	27881.0
14	37082.5
15	46284.0
16	46284.0
17	46284.0
18	68519.5
19	90755.0
20	90755.0
21	90755.0
22	127459.0
23	164163.0
24	164163.0
25	164163.0
26	214375.5
27	264588.0
28	264588.0
29	264588.0
30	308376.0
31	352164.0
32	352164.0
33	352164.0
34	390161.5
35	428159.0
36	428159.0
37	428159.0
38	476569.0
39	524979.0
40	524979.0
41	524979.0
42	712241.5
43	899504.0
44	899504.0
45	899504.0
46	817439.0
47	735374.0
48	735374.0
49	735374.0
50	709989.5
51	684605.0
52	684605.0
53	684605.0
54	630929.0
55	577253.0
56	577253.0
57	577253.0
58	516225.0
59	455197.0
60	455197.0
61	455197.0
62	390029.5
63	324862.0
64	324862.0
65	324862.0
66	251449.0
67	178036.0
68	178036.0
69	178036.0
70	132577.0
71	87118.0
72	87118.0
73	87118.0
74	60780.5
75	34443.0
76	34443.0
77	34443.0
78	23137.0
79	11831.0
80	11831.0
81	11831.0
82	7406.0
83	2981.0
84	2981.0
85	2981.0
86	1734.0
87	487.0
88	487.0
89	487.0
90	282.0
91	77.0
92	77.0
93	77.0
94	54.0
95	31.0
96	31.0
97	31.0
98	24.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08079530211124275
2	0.01809679968039097
3	0.029167188311691593
4	0.035822901415746004
5	0.0451914494811998
6	0.013597874620182042
7	0.017877750894687915
8	0.03403681131693646
9	0.03351446421256764
10	0.020270437630828992
11	0.019057244356165914
12	0.013800073499292556
13	0.020994983614308335
14	0.036412648146485004
15	0.03375036290486324
16	0.01995028940557068
17	0.01995028940557068
18	0.025089510916296236
19	0.014457219856401726
20	0.012098232933445733
21	0.008930450494047686
22	0.016512908460691946
23	0.016849906592542804
24	0.01538396471899158
25	0.10767090312634853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5934751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.625200517746475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.61586987000531	14.405815436029707
2	9.500258595521395	4.1088999419718935
3	4.68567839893572	3.039862048159747
4	3.0502100092924294	2.6384561228874452
5	2.1711111098271463	2.3475356548159567
6	1.7182641153305378	2.2294683621882685
7	1.3401046199380788	2.028602178464357
8	1.1083429825924944	1.9174511392799913
9	0.9523350009866913	1.853499182076494
>10	7.932265804802195	34.375222615514105
>50	0.6930114613878617	10.162203042728382
>100	0.21074877700137307	7.230255543447749
>500	0.011099255388561132	1.6712205020584916
>1k	0.008464178305834482	3.8221594223445523
>5k	9.582088648113936E-4	1.5353910284721952
>10k+	0.0012776118197485246	6.633957779560704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	91878	1.5481357179096478	No Hit
GCTTATGTACTCTGCGTTGATACCA	53954	0.9091198602940544	No Hit
CTTATGTACTCTGCGTTGATACCAC	33548	0.565280666366626	No Hit
GAGTACATAAGCAGTGGTATCAACG	23720	0.3996797843751153	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22194	0.37396682691489497	No Hit
TATGTACTCTGCGTTGATACCACTG	21908	0.36914775362942776	No Hit
GCGTTGATACCACTGCTTATGTACT	18490	0.31155477289611644	No Hit
CCCATGTACTCTGCGTTGATACCAC	15116	0.25470318805287706	No Hit
CATAAGCAGTGGTATCAACGCAGAG	14695	0.2476093773774165	No Hit
GTGGTATCAACGCAGAGTACATGGG	14015	0.2361514408944874	No Hit
GGTATCAACGCAGAGTACTTTTTTT	13699	0.2308268704112439	No Hit
CTGCTTATGTACTCTGCGTTGATAC	12495	0.21053958287382235	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12451	0.20979818698375047	No Hit
GTATCAACGCAGAGTACATAAGCAG	12189	0.20538351145650424	No Hit
ACTCTGCGTTGATACCACTGCTTAT	12004	0.20226627873688383	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	11823	0.19921644564363356	No Hit
TATCAACGCAGAGTACATAAGCAGT	9734	0.16401699077181164	No Hit
CATGTACTCTGCGTTGATACCACTG	9158	0.154311444574507	No Hit
GGTATCAACGCAGAGTACATAAGCA	9049	0.15247480475591985	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8971	0.1511605120417015	No Hit
GTACATGGGAAGCAGTGGTATCAAC	7518	0.12667759776273682	No Hit
GTACATGATAAGCAGTGGTATCAAC	7494	0.12627320000451578	No Hit
ATCATGTACTCTGCGTTGATACCAC	7156	0.12057793157623631	No Hit
GTGGTATCAACGCAGAGTACATAAG	6991	0.11779769698846675	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	6773	0.11412441735129242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6849906592542805E-5	0.0
2	0.0	0.0	0.0	1.6849906592542805E-5	0.0
3	0.0	0.0	0.0	1.6849906592542805E-5	0.0
4	0.0	0.0	0.0	1.6849906592542805E-5	0.0
5	6.739962637017122E-5	0.0	0.0	1.6849906592542805E-5	0.0
6	8.424953296271403E-5	0.0	0.0	1.6849906592542805E-5	0.0
7	8.424953296271403E-5	0.0	0.0	1.6849906592542805E-5	0.0
8	8.424953296271403E-5	0.0	0.0	1.6849906592542805E-5	0.0
9	8.424953296271403E-5	0.0	0.0	5.054971977762841E-5	0.0
10	8.424953296271403E-5	0.0	0.0	5.054971977762841E-5	0.0
11	8.424953296271403E-5	0.0	0.0	5.054971977762841E-5	0.0
12	8.424953296271403E-5	0.0	0.0	6.739962637017122E-5	0.0
13	8.424953296271403E-5	0.0	0.0	8.424953296271403E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	405	0.0	11.02722	3
TGGACCG	315	0.0	10.558066	5
CCGCTAG	95	1.6300939E-4	10.009384	1
TACATAA	20830	0.0	9.99916	2
GTACATA	21085	0.0	9.971169	1
TATACCG	240	0.0	9.898187	5
GTACAAA	1190	0.0	9.828542	1
ACATAAG	21340	0.0	9.671385	3
TAGACCG	80	0.004524838	9.498415	6
CGCCCTA	810	0.0	9.497375	16
CATAAGC	21845	0.0	9.395688	4
CCCCGTA	920	0.0	9.18822	15
GTCCTAA	425	0.0	9.173306	1
GTATTGG	480	0.0	9.11271	1
TGTCCCG	770	0.0	9.004687	11
TGTAGGA	595	0.0	8.943002	2
CTAGAAC	430	0.0	8.839236	3
GTCCCGC	860	0.0	8.835512	12
GCTTATG	12965	0.0	8.793821	1
GTACTGG	1865	0.0	8.769616	1
>>END_MODULE