FastQCFastQC Report
Wed 25 May 2016
SRR1295331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934751
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA942281.5877329984021236No Hit
GCTTATGTACTCTGCGTTGATACCA625021.0531528618471104No Hit
GTATCAACGCAGAGTACTTTTTTTT605061.0195204482883948No Hit
CTTATGTACTCTGCGTTGATACCAC394330.6644423666637405No Hit
GGTATCAACGCAGAGTACTTTTTTT391900.6603478393617526No Hit
TATCAACGCAGAGTACTTTTTTTTT351970.5930661623377291No Hit
GAGTACATAAGCAGTGGTATCAACG248710.4190740268631321No Hit
TATGTACTCTGCGTTGATACCACTG210330.3544040853609528No Hit
GCGTTGATACCACTGCTTATGTACT205310.3459454322514963No Hit
ACGCAGAGTACTTTTTTTTTTTTTT192650.32461345050533713No Hit
GTACTTTTTTTTTTTTTTTTTTTTT186350.31399800935203515No Hit
CCCATGTACTCTGCGTTGATACCAC184630.3110998254181178No Hit
CATAAGCAGTGGTATCAACGCAGAG184460.3108133770060446No Hit
GTGGTATCAACGCAGAGTACATGGG148080.24951341682237382No Hit
ACGCAGAGTACATAAGCAGTGGTAT146390.2466657826082341No Hit
CTGCTTATGTACTCTGCGTTGATAC145730.2455536887731263No Hit
ACTCTGCGTTGATACCACTGCTTAT145220.24469434353690658No Hit
GTATCAACGCAGAGTACATAAGCAG132530.22331181207096978No Hit
TATCAACGCAGAGTACATAAGCAGT110370.18597241906189493No Hit
CATGTACTCTGCGTTGATACCACTG106270.1790639573589524No Hit
GAGTACTTTTTTTTTTTTTTTTTTT102530.17276209229334138No Hit
GGTATCAACGCAGAGTACATAAGCA95110.1602594616016746No Hit
ATCATGTACTCTGCGTTGATACCAC79950.13471500320737972No Hit
AAGCAGTGGTATCAACGCAGAGTAC77010.12976113066917214No Hit
GCAGAGTACTTTTTTTTTTTTTTTT74750.12595305177925747No Hit
GTACATGATAAGCAGTGGTATCAAC74740.1259362018726649No Hit
GTGGTATCAACGCAGAGTACATAAG72120.1215215263454187No Hit
GTACATGGGAAGCAGTGGTATCAAC69370.11688780203246943No Hit
ACATAAGCAGTGGTATCAACGCAGA65270.10997934032952689No Hit
GGTATCAACGCAGAGTACATGGGGG62160.10473901937924607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG1909.094947E-1210.5067545
TAGCCCG1002.3845223E-510.4567215
GCTTTAG3200.09.8165871
CTAAGAC3100.09.50635053
CGGGCCT1106.794828E-59.506194
TATACCG2002.6193447E-109.506115
AGTCCGC900.00111816469.49657910
TACAGGG8450.09.3375992
GTACATA210200.09.2655141
TACATAA209200.09.2109812
GGACCGT2600.09.1314036
TGTCCCG8600.08.94445211
ACATAAG214000.08.9155363
CGTAATA1606.686296E-78.9122042
GCTTATG140250.08.9048691
CGCCCTA8900.08.85628616
TATTCCG1401.0776166E-58.8271025
GCGAACT1201.7723037E-48.70519814
GTCCCGC9300.08.67966912
GCGATAA3200.08.60627611
Produced by FastQC (version 0.11.5)