FastQCFastQC Report
Wed 25 May 2016
SRR1295330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119124
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG60520.11822335227667857No Hit
GTATCAACGCAGAGTACTTTTTTTT58620.11451177974981658No Hit
ATTCCATTCCATTCCATTCCATTCC53130.10378728860641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG957.37291E-813.0036124
GACGGAT1001.9350955E-611.3985997
CTGTCCG700.001494433310.8553849
GTTTACG803.74857E-410.695281
TACACCG1502.438901E-810.1361495
GCGTCAG1909.822543E-1110.0072791
ACGGATC1201.7045393E-59.4989258
CTTAGAC4000.09.2649833
TAATACG1252.7478589E-59.1225344
TTAGACT5000.09.1225344
TTATACT8350.09.1043254
GGTATCA45350.09.0771661
TATACTG11950.08.667685
CCTACAC5850.08.2842713
TCTAGAC4500.08.2355413
CTTACAC6500.08.186813
AGGACTG7900.08.1794875
GTATCGA1050.00448600588.1487851
TAGACTG7950.08.1280445
GTCCTAG4800.08.1204911
Produced by FastQC (version 0.11.5)