FastQCFastQC Report
Wed 25 May 2016
SRR1295330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119124
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT151700.2963397643815621No Hit
GGTATCAACGCAGAGTACTTTTTTT123160.24058803810964535No Hit
TATCAACGCAGAGTACTTTTTTTTT90830.17743270137625108No Hit
GTGGTATCAACGCAGAGTACATGGG67250.13137013285866878No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57490.11230437082594599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG1354.6820787E-911.2678825
AGCGTCA4600.010.1275133
GGTATCA53800.09.9247851
AACGATT1151.0324793E-59.90910317
CAGCGTC4850.09.8015062
TATACCG1401.0490312E-69.5072765
GACCGCT1106.8727415E-59.4962257
GCAGCGT4250.09.40591051
TAGACTG7000.08.9640035
ATGACCG850.00739305338.9480235
GTCTTAG5050.08.858231
GTAGGAC3550.08.8379223
GGACAGT5400.08.7931446
TCTATAC7400.08.6081073
TATACTG11250.08.535425
CTAGACT4800.08.5170174
GTATAGA8600.08.4111551
CTAGGAC5450.08.3735443
CTGTCGC4100.08.338239
TAGGACA5400.08.2749324
Produced by FastQC (version 0.11.5)