FastQCFastQC Report
Wed 25 May 2016
SRR1295329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361490
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51430.11791841778841633No Hit
GTGGTATCAACGCAGAGTACATGGG48730.11172787281410711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG550.003063580712.09427455
CTAGGAC3600.09.7664953
GGTATCA32750.09.5208661
GGACCGA1054.5016405E-49.0464026
TAGACAG5950.08.94366555
GTGGTAT17300.08.9019031
GTTAGAC1951.6440026E-88.7715723
CGTCAGA2304.0381565E-108.6761292
GGACCGT1107.158427E-48.63520056
TTAGACA5200.08.5889294
TTAGACT4450.08.5417024
CTAGCAC4450.08.5416053
GCTAGAC2409.295036E-108.3147183
TGGTATC17350.08.27012352
GTATCAA56500.08.1939721
TGGACCG1401.0365432E-48.1451245
GACGGCT1050.00452070368.1417627
CTGTGCG1752.447463E-68.1416689
GACTGCG1659.770889E-68.0595217
GACCGTG1304.1945136E-48.0373797
Produced by FastQC (version 0.11.5)