##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295329_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4361490
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01622495981878	31.0	30.0	33.0	26.0	34.0
2	29.848830560198465	31.0	30.0	34.0	25.0	34.0
3	30.204105019156298	31.0	30.0	34.0	26.0	34.0
4	33.56507294525495	35.0	33.0	37.0	27.0	37.0
5	33.76851420042233	35.0	35.0	37.0	28.0	37.0
6	34.03718981357289	36.0	35.0	37.0	29.0	37.0
7	33.90096618357488	36.0	35.0	37.0	28.0	37.0
8	33.889324519831526	36.0	35.0	37.0	28.0	37.0
9	34.957467516834846	38.0	35.0	39.0	27.0	39.0
10	34.84067784174674	38.0	34.0	39.0	27.0	39.0
11	35.271798399170926	38.0	35.0	39.0	27.0	39.0
12	35.263805029932435	38.0	35.0	39.0	27.0	39.0
13	35.17544004457192	38.0	35.0	39.0	27.0	39.0
14	35.35978094641969	39.0	34.0	41.0	25.0	41.0
15	35.98798231796932	39.0	35.0	41.0	27.0	41.0
16	36.06349779547815	39.0	35.0	41.0	27.0	41.0
17	35.866377774567866	39.0	35.0	41.0	27.0	41.0
18	35.83471978612813	39.0	34.0	41.0	27.0	41.0
19	35.90372579095676	39.0	34.0	41.0	27.0	41.0
20	35.90136352485045	39.0	34.0	41.0	27.0	41.0
21	35.88629894829519	39.0	34.0	41.0	27.0	41.0
22	35.622549862546975	39.0	34.0	41.0	25.0	41.0
23	35.30423066429133	39.0	34.0	40.0	25.0	41.0
24	35.63985587494182	39.0	34.0	41.0	26.0	41.0
25	34.28846540975676	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2936938123877333
1101	2	0.45393909334906724
1101	3	0.35168173175348016
1101	4	0.35516221689076843
1101	5	0.17406934728602863
1101	6	0.19014838656269006
1101	7	0.17135454927978344
1101	8	0.21645160892283144
1101	9	0.04176038215721434
1101	10	0.501213398484154
1101	11	0.4111826355657726
1101	12	0.4060475056822881
1101	13	0.36494581833863293
1101	14	0.8756782973694257
1101	15	0.6546166893227294
1101	16	0.48804011830732463
1101	17	0.6705360332195056
1101	18	0.7026189934322602
1101	19	0.8068591270098509
1101	20	0.7769560742716379
1101	21	0.8814816529841991
1101	22	0.636305147297989
1101	23	-1.6046621522478048
1101	24	0.054180245851526365
1101	25	0.4509578918285513
1102	1	0.17210047723589383
1102	2	0.20572160277096074
1102	3	0.1629226651997584
1102	4	0.20151374291409496
1102	5	-0.10776667313472643
1102	6	0.017395782497224843
1102	7	-0.08238272997167684
1102	8	-0.0129843220137289
1102	9	0.0638288948789878
1102	10	0.6243006082129199
1102	11	0.2855731119549887
1102	12	0.2454771684767394
1102	13	0.17524159475876644
1102	14	0.6558759331523447
1102	15	0.41649128657824974
1102	16	0.22924013451810765
1102	17	0.6063904767049948
1102	18	0.5134812517902532
1102	19	0.46212376452118065
1102	20	0.5754734372835344
1102	21	0.6529233202558515
1102	22	0.5038623324844096
1102	23	0.4991958894319879
1102	24	0.5452994892893983
1102	25	0.40173431633350276
1103	1	-0.40106854030501893
1103	2	-1.6958506833035933
1103	3	-0.4915092280576765
1103	4	-0.14315882939742153
1103	5	-0.36285059768781736
1103	6	-0.17312897498254642
1103	7	-0.19363611584988405
1103	8	0.07423777643544582
1103	9	0.6639997530836439
1103	10	0.6324055160242708
1103	11	0.4132790199896377
1103	12	0.18649562625081018
1103	13	0.08221882300132677
1103	14	-1.2098258947400709
1103	15	-0.2973343260148411
1103	16	-0.16420013359230978
1103	17	0.2750375978508899
1103	18	0.44261351361728885
1103	19	0.35864161315196696
1103	20	0.3772604460469182
1103	21	0.5173254026239462
1103	22	0.05070739166549032
1103	23	0.8497940216323983
1103	24	0.576328022789454
1103	25	0.4409641207472461
1104	1	-0.19470292680925994
1104	2	-0.10116966518506842
1104	3	-0.5586656082829613
1104	4	-0.4541556148043995
1104	5	-0.3367632346694478
1104	6	-0.2601139514857138
1104	7	-0.3206045868225331
1104	8	-0.2396459303578311
1104	9	-2.2516782209937176
1104	10	-0.8195392919842845
1104	11	-0.7699035605340256
1104	12	-0.346508851309558
1104	13	-0.15294641390181596
1104	14	-1.8486489120284588
1104	15	-0.8012248522309093
1104	16	-0.45896109015664166
1104	17	-0.1372283230535274
1104	18	0.063799923515937
1104	19	0.012755531357953487
1104	20	0.25827079695336863
1104	21	0.3129198540156537
1104	22	0.46466725471854176
1104	23	0.7436621702090349
1104	24	0.5408892048180292
1104	25	0.3754728744312317
1105	1	-0.2824042301894458
1105	2	-0.14413406186586641
1105	3	-1.5665990606647568
1105	4	-0.6932514278990922
1105	5	-0.5260426509945049
1105	6	-0.2810934786083834
1105	7	-0.23481861745223398
1105	8	-0.2955112850517949
1105	9	0.17057688576193897
1105	10	0.35003099796415427
1105	11	-0.23521810014221955
1105	12	-0.33828732334190903
1105	13	-0.4385958800403884
1105	14	0.3753190216702791
1105	15	0.028485253279022515
1105	16	-0.49961431863070516
1105	17	0.1027310874201035
1105	18	0.01710708788562698
1105	19	0.0034037742542238902
1105	20	-0.4279561780165153
1105	21	-0.0904743489217239
1105	22	0.042815624614426895
1105	23	0.12985675915331996
1105	24	-0.01392171590804736
1105	25	0.09126634869202377
1106	1	-1.6977240049557878
1106	2	-1.092023454020918
1106	3	-1.2746815178568554
1106	4	-1.4722152808037237
1106	5	-1.1702971726889189
1106	6	-0.8290768727996678
1106	7	-0.7646598950979708
1106	8	-0.8026863328179843
1106	9	-0.3535556514814715
1106	10	-1.9446125644020142
1106	11	-1.3712791117190548
1106	12	-1.0227416119515595
1106	13	-1.0899302231970935
1106	14	-0.340831112643734
1106	15	-1.1258929747396493
1106	16	-1.4991460856122814
1106	17	-0.7685965538584085
1106	18	-0.694777115349126
1106	19	-0.6592792833819274
1106	20	-0.4211274301579948
1106	21	-0.386776695250461
1106	22	-0.4383029591369336
1106	23	-0.6599554875516276
1106	24	-0.9791699894931725
1106	25	-1.74407364821176
1107	1	-0.0379791137535328
1107	2	0.0546852152204238
1107	3	-0.06010819592750849
1107	4	0.25081091496389973
1107	5	-0.09486077852326957
1107	6	0.01203620640191616
1107	7	-0.10691353965930261
1107	8	-0.16666579744139653
1107	9	0.445721137289496
1107	10	0.06749527098980224
1107	11	0.07033660818414234
1107	12	-0.07726042146418877
1107	13	0.01715871432793392
1107	14	-1.134029020806551
1107	15	-0.519192852761293
1107	16	-0.2355891370634069
1107	17	-0.02127100847662433
1107	18	0.2905390150669547
1107	19	0.18969731593329442
1107	20	0.25347703708933267
1107	21	0.4646614404271716
1107	22	-0.26918124431820445
1107	23	0.5662173948205549
1107	24	0.38235056267642875
1107	25	0.08002273968703832
1108	1	-0.16025142516497937
1108	2	0.1677675800685492
1108	3	-0.06741116548227666
1108	4	-0.4177010154084826
1108	5	-0.3984728405173499
1108	6	-0.24394459134520474
1108	7	-0.24597769648575962
1108	8	-0.25559706735759846
1108	9	0.2860151443066954
1108	10	0.17510921981652672
1108	11	-0.010000965174434384
1108	12	-0.001816544454939617
1108	13	-0.19652413441237826
1108	14	-0.0017780390676804814
1108	15	-0.05400947484704233
1108	16	-0.0908343934452418
1108	17	0.22561330580298034
1108	18	0.21336336918221122
1108	19	0.23384173852408452
1108	20	0.27265769776823845
1108	21	0.2867886922469296
1108	22	0.16463433480436152
1108	23	-0.07044451090640536
1108	24	0.04196646441165797
1108	25	-0.7410748507365952
1109	1	-0.8727286286282023
1109	2	-0.7236990040411833
1109	3	-0.802423158847386
1109	4	-0.6750724644166439
1109	5	-1.048153864421792
1109	6	-1.1815104985064977
1109	7	-0.8521537957854264
1109	8	-1.2404915935734309
1109	9	-0.823394304989364
1109	10	-1.226288606302326
1109	11	-1.2790850020809543
1109	12	-1.1424878411991344
1109	13	-1.2224686291909421
1109	14	-0.6047590404121337
1109	15	-1.2547290980165897
1109	16	-1.2947318065313524
1109	17	-1.380798769679629
1109	18	-2.0371986211316653
1109	19	-2.044377285561943
1109	20	-1.7303847109664119
1109	21	-1.4679615457386035
1109	22	-1.577583564897047
1109	23	-1.176903853919626
1109	24	-1.9197759660324607
1109	25	-1.083960151810352
1110	1	0.11556602072761635
1110	2	0.20074437592858274
1110	3	0.06172920480481281
1110	4	0.4456097256086764
1110	5	0.3176626049368849
1110	6	0.21667049398480742
1110	7	0.23287111914300596
1110	8	0.06457599711255568
1110	9	0.4315809045013239
1110	10	0.43689515451436023
1110	11	0.3444837114875696
1110	12	0.3140712236667724
1110	13	0.2740781181822598
1110	14	-2.3297436435462515
1110	15	-0.5380575582677949
1110	16	-0.11515147221717825
1110	17	-0.09969678954936256
1110	18	-2.8272146089883634
1110	19	-0.518863279079703
1110	20	-1.857494392640092
1110	21	-0.2647838814587118
1110	22	-1.7051542194038163
1110	23	-0.46783245600862244
1110	24	0.2198658313261248
1110	25	0.17007507124201737
1111	1	-0.11518631675165025
1111	2	-1.277147225778691
1111	3	-0.3274302221027625
1111	4	0.11914194462432448
1111	5	0.0842496450148218
1111	6	0.09829855003782484
1111	7	-0.013540358821593657
1111	8	0.10824800774504695
1111	9	-1.7249333910420077
1111	10	-0.2837743038867444
1111	11	-0.1003278102797438
1111	12	-0.01799171381156839
1111	13	0.050120635743375885
1111	14	0.9844124749132348
1111	15	0.31000701768007133
1111	16	0.3940801052023133
1111	17	0.031965642338185773
1111	18	0.1809992474430686
1111	19	0.036723824532828075
1111	20	0.4531730047605649
1111	21	-0.5433278337137537
1111	22	-0.16696454227839297
1111	23	0.06684816898589219
1111	24	-0.4004821833209249
1111	25	-1.719252613882567
1112	1	0.3164384597518968
1112	2	0.4554919424987389
1112	3	0.18945599477912722
1112	4	0.5332279308402761
1112	5	0.38650139183307175
1112	6	0.29447088275583866
1112	7	0.3505627409849055
1112	8	0.253300254408515
1112	9	-0.15622272874708898
1112	10	0.6422056381154704
1112	11	0.3153850126965665
1112	12	0.2261082593740369
1112	13	0.25566467295637807
1112	14	0.8186825797585655
1112	15	0.43233628813174363
1112	16	0.33497226887573817
1112	17	-3.4529476249910047
1112	18	-0.7423318875927833
1112	19	-0.8940097821900252
1112	20	0.12179380258725558
1112	21	-0.24984839124616087
1112	22	-1.4278643465022256
1112	23	-1.2068139633902675
1112	24	-0.1360401453852873
1112	25	0.3045255451174924
1113	1	-0.28600099630868314
1113	2	-0.06960323097451848
1113	3	-0.027078263669384484
1113	4	-0.3540172127380572
1113	5	-0.14245701340548322
1113	6	-0.17942549549491105
1113	7	-0.3267055924504234
1113	8	-0.1901988138147459
1113	9	0.1816530854416527
1113	10	0.3947470759424263
1113	11	-0.08456899258892037
1113	12	-0.1373185709609217
1113	13	-0.4504276853005038
1113	14	-1.5671822298479015
1113	15	-1.8062885265462114
1113	16	-0.4874302190254056
1113	17	-0.39760378494786863
1113	18	-0.6474527255092752
1113	19	-0.16395219111045378
1113	20	0.27390307644087386
1113	21	-1.6508979006611852
1113	22	-2.009456121534143
1113	23	-0.7658651268254602
1113	24	-2.385011574934751
1113	25	-0.5505650202649193
1114	1	0.2003167250275908
1114	2	0.272262610034506
1114	3	0.089511577409219
1114	4	0.44189240069877656
1114	5	0.2692877346081133
1114	6	0.25434236397484256
1114	7	0.3047314076682852
1114	8	0.24703042366954975
1114	9	0.5981268107853737
1114	10	0.42129293341822205
1114	11	0.41501656120988883
1114	12	0.44694846410236977
1114	13	0.29238758875551696
1114	14	1.1252127540744183
1114	15	0.5772210250029204
1114	16	0.5778928619446475
1114	17	-0.15585143524450729
1114	18	0.10542015476823252
1114	19	-0.35263253959803365
1114	20	-1.5265355694390905
1114	21	-0.591449268828022
1114	22	-1.3457199657797574
1114	23	-2.705480184091428
1114	24	-1.9388542733606755
1114	25	-1.3428484049119476
1115	1	0.5010826914037594
1115	2	0.5714646632380216
1115	3	0.47037901352278055
1115	4	0.7032130911731969
1115	5	0.6486720661189693
1115	6	0.5149863205077025
1115	7	0.6209973858983986
1115	8	0.6291075671017339
1115	9	0.8552684573129454
1115	10	1.0446569003623694
1115	11	0.664306964107702
1115	12	0.6323244332881757
1115	13	0.5512133020222976
1115	14	1.2578412066609204
1115	15	0.7476250123661146
1115	16	0.7984219714412646
1115	17	0.725048838808533
1115	18	0.9723743425639171
1115	19	0.4541125043195535
1115	20	0.9646382885345517
1115	21	-0.13024842926632374
1115	22	0.8825543576250183
1115	23	1.0650163144560878
1115	24	0.9245920557763654
1115	25	1.2167954320726508
1116	1	0.6928181408764331
1116	2	0.8140312138070023
1116	3	0.749107904063596
1116	4	1.006794009231406
1116	5	0.9149905932590698
1116	6	0.7357693350530212
1116	7	0.8653828224742952
1116	8	0.7692210867681126
1116	9	0.9916346224205199
1116	10	0.9798814604701604
1116	11	0.7990783146805285
1116	12	0.7547589360894165
1116	13	0.36120303666913145
1116	14	1.349880851831358
1116	15	1.1008989711316772
1116	16	1.009640344300685
1116	17	1.1860778653662791
1116	18	1.1903057277097133
1116	19	1.2946515775460057
1116	20	1.200255499402445
1116	21	0.6334304642383444
1116	22	0.6176153649833864
1116	23	-0.273484767751647
1116	24	0.2604672221928368
1116	25	-0.3327381612255138
1201	1	0.39776612399548483
1201	2	0.6528627150992463
1201	3	0.4496058266598624
1201	4	0.38420289790219897
1201	5	0.330476820235873
1201	6	0.28786481383733076
1201	7	0.3015043751291202
1201	8	0.34574029048454236
1201	9	0.5852863546808038
1201	10	0.9088888049920314
1201	11	0.5672068519920046
1201	12	0.5233042627488871
1201	13	0.500319976506205
1201	14	1.0209431085134
1201	15	0.6950909919384287
1201	16	0.475143255937418
1201	17	0.5560727945382595
1201	18	0.5310222684235555
1201	19	0.793743653968292
1201	20	0.7861720106727716
1201	21	0.7674407531119698
1201	22	0.8113669598405835
1201	23	0.457853767269782
1201	24	0.8225628645522463
1201	25	1.0076840261006907
1202	1	0.29980333264384385
1202	2	0.5740441205746656
1202	3	0.37390122489636113
1202	4	0.29444290284460095
1202	5	0.1361857679081524
1202	6	0.1927679362875736
1202	7	0.15453641342424618
1202	8	0.265902005158587
1202	9	0.7107448800116956
1202	10	-0.07426787993171757
1202	11	0.2709006401119112
1202	12	0.25394686075019735
1202	13	0.22948741542381867
1202	14	0.8176242189357055
1202	15	0.6462627256364755
1202	16	0.4501316135886668
1202	17	-1.4707513900180018
1202	18	0.03367823648538604
1202	19	0.411907081441683
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2210	20	0.9841275038407105
2210	21	1.0842918823759504
2210	22	1.2141040929096647
2210	23	1.5306655799667581
2210	24	1.1916830960735254
2210	25	1.1665704071172627
2211	1	0.1122681710655371
2211	2	-0.5230220461596389
2211	3	-0.09550330854723299
2211	4	0.29153894391731683
2211	5	0.26461340878735484
2211	6	0.14077692797090435
2211	7	0.1938357744208048
2211	8	0.3212734078080288
2211	9	0.7652775635824156
2211	10	0.7576085698929376
2211	11	0.44620014563980703
2211	12	0.23550881904959198
2211	13	0.22071783650168442
2211	14	-0.723368799657294
2211	15	0.02321949267939516
2211	16	0.2299341533829704
2211	17	0.24194449473668556
2211	18	0.17276792938857
2211	19	0.5076853313098653
2211	20	0.625811324464749
2211	21	0.6293082335831741
2211	22	-0.01799581111362869
2211	23	0.5136069132271359
2211	24	-0.1479922305172039
2211	25	0.3794698749440357
2212	1	0.050074303572316126
2212	2	-0.9748716251295839
2212	3	-0.2530848655600906
2212	4	0.15021771152095198
2212	5	0.2469776513800781
2212	6	0.18069507204060642
2212	7	0.1338245405713252
2212	8	0.14594574767595958
2212	9	-2.1669801538304085
2212	10	0.011374764554737737
2212	11	0.006210244882048244
2212	12	-0.11798662944249827
2212	13	-0.021775003714012087
2212	14	0.6161070084945663
2212	15	0.29136376042492174
2212	16	0.30288446889043286
2212	17	0.3285073328754322
2212	18	0.21578243726072088
2212	19	-0.5662254601748202
2212	20	0.19472033399718214
2212	21	0.3100871245413117
2212	22	-1.7571547420280922
2212	23	-0.35697320361843765
2212	24	0.18068151840915192
2212	25	-0.5064462948265458
2213	1	0.2805028323991223
2213	2	0.5755465829605306
2213	3	0.35594467857143997
2213	4	0.49559107249598355
2213	5	0.5283790133466795
2213	6	0.306092227411078
2213	7	0.2753618177381867
2213	8	0.3460689354131148
2213	9	0.8211359646030374
2213	10	0.8660136028966718
2213	11	0.22385164358733078
2213	12	0.21589257764199488
2213	13	0.2535473797027876
2213	14	-1.4679292668412387
2213	15	-0.22234644930085068
2213	16	0.21073499322071143
2213	17	-1.7814136061823334
2213	18	-0.6293069991351317
2213	19	-0.25919103005730193
2213	20	0.31381054068457814
2213	21	0.02286670475021424
2213	22	0.571736490813322
2213	23	1.0276475936846552
2213	24	0.8287967617809215
2213	25	-0.7387516447936378
2214	1	0.3419582940636374
2214	2	0.257812700525875
2214	3	0.20029368983139761
2214	4	0.6238906556493404
2214	5	0.6081223834192002
2214	6	0.49163119337755745
2214	7	0.46427709873736234
2214	8	0.4144872285260064
2214	9	0.7938262888215633
2214	10	0.7317710459425868
2214	11	0.1344856623087125
2214	12	0.07180413561947319
2214	13	0.2311944408572586
2214	14	1.052058870700776
2214	15	0.5801035498443312
2214	16	0.5545429570282465
2214	17	0.5217891533036791
2214	18	0.5777263297433421
2214	19	-0.589951781155392
2214	20	0.30370725032722845
2214	21	0.5493032921034953
2214	22	0.8104257680488018
2214	23	1.2391846572377219
2214	24	1.017335923037983
2214	25	1.0179192465306954
2215	1	0.050857415131858374
2215	2	0.40739602578437584
2215	3	0.3455646111223558
2215	4	0.5437513388459578
2215	5	0.5196051369919843
2215	6	0.41832228659133364
2215	7	0.38324923052662996
2215	8	0.3319247810781931
2215	9	0.49718918130250955
2215	10	0.8018124784468625
2215	11	0.5062752768303724
2215	12	0.37259252196177783
2215	13	0.22853334775431478
2215	14	1.177512513725425
2215	15	0.6046046846652118
2215	16	0.5974295502088864
2215	17	0.6941722746780883
2215	18	0.6249420853925116
2215	19	-0.16597844417811558
2215	20	-1.4468605419266325
2215	21	-1.7348124496167188
2215	22	-0.004262628779947875
2215	23	0.685862151299446
2215	24	0.8161819846695124
2215	25	0.22167679964591258
2216	1	0.1850479507080287
2216	2	-0.029387310966857427
2216	3	0.12860593092427663
2216	4	0.4929663219455733
2216	5	0.6226715861622623
2216	6	0.34577952554622016
2216	7	0.4545447254575521
2216	8	0.3767808493418059
2216	9	0.5803160700389469
2216	10	-1.1504470306872534
2216	11	-0.1747147411682448
2216	12	0.08187271233704507
2216	13	0.2886277650579743
2216	14	-0.25847405340923046
2216	15	-0.18223462582822947
2216	16	0.23302460303364825
2216	17	0.5983916351179701
2216	18	0.49838646402359643
2216	19	-0.925497301093742
2216	20	-0.6234734903177426
2216	21	-0.5183354954543944
2216	22	-0.3717828681231339
2216	23	-4.2908408422599855
2216	24	-1.7137211517130027
2216	25	-0.7441249614918419
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	23.0
4	45.0
5	69.0
6	101.0
7	383.0
8	362.0
9	1028.0
10	2311.0
11	4781.0
12	8360.0
13	12970.0
14	17984.0
15	22617.0
16	25883.0
17	28487.0
18	30100.0
19	31272.0
20	31806.0
21	32763.0
22	34087.0
23	36192.0
24	39048.0
25	42957.0
26	48160.0
27	55064.0
28	63402.0
29	77541.0
30	95011.0
31	117418.0
32	152917.0
33	202077.0
34	271793.0
35	371302.0
36	520291.0
37	770805.0
38	1212078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.722757732260376	21.25165473710877	13.627415503139897	23.39817202749095
2	15.564499835686881	21.557880986851053	40.28237547462182	22.595243702840243
3	18.1843244071401	26.34996096376832	30.20778192553667	25.257932703554914
4	12.633770058627618	17.668726431972694	38.25491086583542	31.442592643564264
5	13.30494520493268	37.69681332056686	35.256096458771	13.742145015729468
6	30.53150616575038	38.68035467683873	17.878818593886947	12.909320563523947
7	25.766122531445383	32.85036086482802	23.755617765589566	17.62789883813703
8	23.74237046184107	37.29657240733316	21.297569648635285	17.663487482190483
9	24.411328456760266	17.277774987276462	22.371204411713773	35.9396921442495
10	14.948676797527568	30.002481420308108	33.96968080495257	21.079160977211757
11	31.9165511591875	25.806381333379814	23.95614415700333	18.320923350429357
12	20.773802548628097	27.439155970178174	31.77745127558678	20.009590205606944
13	26.12109700731956	24.39865222508538	27.216554000438492	22.263696767156564
14	20.918706826813665	23.47018092800518	28.273902838842353	27.337209406338797
15	22.44247850125527	31.207938859024104	24.417009822427502	21.932572817293124
16	21.998971659165097	31.277775407976566	25.949185652214396	20.774067280643937
17	20.25713495491504	30.379131985485845	28.59341524261117	20.770317816987944
18	20.40589842287962	30.155615876883335	29.34935069462282	20.089135005614224
19	21.04110391982533	29.079989607123114	29.491187037615013	20.387719435436548
20	22.06912413969755	28.76919277356259	28.913638051183483	20.248045035556377
21	22.485966937028184	29.036441821978737	27.973439738258925	20.504151502734153
22	21.501072296052776	28.660918707300787	29.321976840606723	20.516032156039714
23	21.851364580865713	28.86491739864969	28.64265659903826	20.641061421446334
24	21.427742605390275	28.23366838319333	28.990213596578933	21.348375414837466
25	22.060228715887202	28.470452462484154	28.407814157266902	21.061504664361742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3321.0
1	3321.0
2	3608.0
3	3895.0
4	3895.0
5	3895.0
6	5927.0
7	7959.0
8	7959.0
9	7959.0
10	12688.0
11	17417.0
12	17417.0
13	17417.0
14	29575.5
15	41734.0
16	41734.0
17	41734.0
18	66060.0
19	90386.0
20	90386.0
21	90386.0
22	129147.0
23	167908.0
24	167908.0
25	167908.0
26	218439.5
27	268971.0
28	268971.0
29	268971.0
30	323205.0
31	377439.0
32	377439.0
33	377439.0
34	422480.5
35	467522.0
36	467522.0
37	467522.0
38	496987.0
39	526452.0
40	526452.0
41	526452.0
42	526937.0
43	527422.0
44	527422.0
45	527422.0
46	505767.5
47	484113.0
48	484113.0
49	484113.0
50	457041.0
51	429969.0
52	429969.0
53	429969.0
54	389820.5
55	349672.0
56	349672.0
57	349672.0
58	302509.0
59	255346.0
60	255346.0
61	255346.0
62	211395.0
63	167444.0
64	167444.0
65	167444.0
66	130346.0
67	93248.0
68	93248.0
69	93248.0
70	69644.0
71	46040.0
72	46040.0
73	46040.0
74	33498.5
75	20957.0
76	20957.0
77	20957.0
78	14827.0
79	8697.0
80	8697.0
81	8697.0
82	6169.0
83	3641.0
84	3641.0
85	3641.0
86	2505.5
87	1370.0
88	1370.0
89	1370.0
90	899.5
91	429.0
92	429.0
93	429.0
94	275.0
95	121.0
96	121.0
97	121.0
98	69.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08132541860694396
2	0.02093321319090494
3	0.03297038397428402
4	0.04058246149824945
5	0.04943264801707673
6	0.018067220147243258
7	0.02276744873884842
8	0.03352065463866706
9	0.03349772669431777
10	0.02485389167463413
11	0.02343235912497793
12	0.018136003980291138
13	0.025633441782510105
14	0.0362032241275344
15	0.03365822230476282
16	0.02448704456504543
17	0.02421190923285391
18	0.026092000669495973
19	0.01859456286727701
20	0.015338794769677335
21	0.012358162004269183
22	0.017287670039367282
23	0.019419968863851576
24	0.017104246484572934
25	0.10847210471650742
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4361490.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.04751983862745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85215346451824	70.32346997317168
2	8.99868810142702	14.406453286412003
3	1.666470008649438	4.001903732335307
4	0.5543407740061221	1.7749441641846064
5	0.2747703818981579	1.099734379803002
6	0.14499225436272103	0.6963762214528337
7	0.1040781741720213	0.58318397982621
8	0.07279281373777576	0.4661507361427294
9	0.05101316632878732	0.36751296993613247
>10	0.2602731879496736	3.5979783329997876
>50	0.013335938450376578	0.7220551540301511
>100	0.0061720288077356765	0.8591788433286465
>500	5.460754041663386E-4	0.30494854396745397
>1k	3.448894964130562E-4	0.677788329484121
>5k	2.8740791367739947E-5	0.11832135292542824
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5143	0.11791841778841633	No Hit
GTGGTATCAACGCAGAGTACATGGG	4873	0.11172787281410711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2927944349293475E-5	0.0
2	0.0	2.2927944349293475E-5	0.0	2.2927944349293475E-5	0.0
3	0.0	2.2927944349293475E-5	0.0	2.2927944349293475E-5	0.0
4	0.0	2.2927944349293475E-5	0.0	2.2927944349293475E-5	0.0
5	0.0	2.2927944349293475E-5	0.0	4.585588869858695E-5	0.0
6	0.0	2.2927944349293475E-5	0.0	4.585588869858695E-5	0.0
7	0.0	2.2927944349293475E-5	0.0	4.585588869858695E-5	0.0
8	0.0	2.2927944349293475E-5	0.0	4.585588869858695E-5	0.0
9	0.0	2.2927944349293475E-5	0.0	6.878383304788043E-5	0.0
10	2.2927944349293475E-5	2.2927944349293475E-5	0.0	6.878383304788043E-5	0.0
11	2.2927944349293475E-5	6.878383304788043E-5	0.0	6.878383304788043E-5	0.0
12	2.2927944349293475E-5	6.878383304788043E-5	0.0	6.878383304788043E-5	0.0
13	2.2927944349293475E-5	6.878383304788043E-5	0.0	6.878383304788043E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	55	0.0030635807	12.0942745	5
CTAGGAC	360	0.0	9.766495	3
GGTATCA	3275	0.0	9.520866	1
GGACCGA	105	4.5016405E-4	9.046402	6
TAGACAG	595	0.0	8.9436655	5
GTGGTAT	1730	0.0	8.901903	1
GTTAGAC	195	1.6440026E-8	8.771572	3
CGTCAGA	230	4.0381565E-10	8.676129	2
GGACCGT	110	7.158427E-4	8.6352005	6
TTAGACA	520	0.0	8.588929	4
TTAGACT	445	0.0	8.541702	4
CTAGCAC	445	0.0	8.541605	3
GCTAGAC	240	9.295036E-10	8.314718	3
TGGTATC	1735	0.0	8.2701235	2
GTATCAA	5650	0.0	8.193972	1
TGGACCG	140	1.0365432E-4	8.145124	5
GACGGCT	105	0.0045207036	8.141762	7
CTGTGCG	175	2.447463E-6	8.141668	9
GACTGCG	165	9.770889E-6	8.059521	7
GACCGTG	130	4.1945136E-4	8.037379	7
>>END_MODULE