FastQCFastQC Report
Wed 25 May 2016
SRR1295329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361490
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130450.2990950340365334No Hit
GGTATCAACGCAGAGTACTTTTTTT106320.2437699043216882No Hit
TATCAACGCAGAGTACTTTTTTTTT77230.17707251420959352No Hit
GTGGTATCAACGCAGAGTACATGGG54990.12608076597676482No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53170.12190788010519342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3150.013.2788263
CAGCGTC3550.011.782622
GGTATCA39450.011.4569241
GCAGCGT3650.010.9490851
GGACGGT1054.123351E-59.9491856
CTAGGAC4550.09.6108993
TATACCG1002.7390372E-49.5063245
CTAGTAC2950.09.3453053
CTAGAAC4850.09.2124193
TGGTATC17250.09.0931092
TAGACAG6700.09.0806675
TGGACCG950.00181381859.0059915
TGGACTG6550.08.9983515
CTAATAC5850.08.9376723
GTCTAAT4050.08.9279151
GACTGCG1304.38958E-58.7663157
GTAGGAC2751.8189894E-128.6422113
GTGGTAT18950.08.5863451
GTATTAG5450.08.555021
TTATACT6450.08.5484194
Produced by FastQC (version 0.11.5)