FastQCFastQC Report
Wed 25 May 2016
SRR1295328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389789
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1408694.155686386379801No Hit
GCTTATGTACTCTGCGTTGATACCA827532.4412433930253474No Hit
CTTATGTACTCTGCGTTGATACCAC523701.5449339177158226No Hit
GAGTACATAAGCAGTGGTATCAACG362101.0682080802079421No Hit
TATGTACTCTGCGTTGATACCACTG339481.0014782631013317No Hit
CCCATGTACTCTGCGTTGATACCAC298800.8814707936098678No Hit
GCGTTGATACCACTGCTTATGTACT277740.8193430328554373No Hit
CATAAGCAGTGGTATCAACGCAGAG229290.676413782686769No Hit
CATGTACTCTGCGTTGATACCACTG207460.6120144941174804No Hit
ACTCTGCGTTGATACCACTGCTTAT201000.592957260761658No Hit
CTGCTTATGTACTCTGCGTTGATAC195820.5776760736435218No Hit
ACGCAGAGTACATAAGCAGTGGTAT186470.5500932358916735No Hit
GTATCAACGCAGAGTACATAAGCAG180080.5312425050644746No Hit
GTACATGATAAGCAGTGGTATCAAC165690.48879148525173693No Hit
ATCATGTACTCTGCGTTGATACCAC155490.45870111679517517No Hit
TATCAACGCAGAGTACATAAGCAGT152330.4493790026458874No Hit
GTATCAACGCAGAGTACTTTTTTTT146080.4309412768759353No Hit
GTACATGGGAAGCAGTGGTATCAAC140150.41344756266540483No Hit
GGTATCAACGCAGAGTACATAAGCA139900.4127100536346067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA134700.3973698657940066No Hit
AAGCAGTGGTATCAACGCAGAGTAC118450.34943177879213133No Hit
GTGGTATCAACGCAGAGTACATAAG108470.31999041828267183No Hit
GTGGTATCAACGCAGAGTACATGGG107610.3174533872167265No Hit
GGTATCAACGCAGAGTACTTTTTTT94130.2776869002760939No Hit
GCAGTGGTATCAACGCAGAGTACAT90140.2659162561445565No Hit
ACATAAGCAGTGGTATCAACGCAGA84440.24910105024236023No Hit
GTACTCTGCGTTGATACCACTGCTT84110.24812753832170678No Hit
GTACATGGGATAAGCAGTGGTATCA83960.24768503290322788No Hit
TATCAACGCAGAGTACTTTTTTTTT83360.2459150112293125No Hit
CCACTGCTTATGTACTCTGCGTTGA73950.21815517131007267No Hit
GCTTATCCCATGTACTCTGCGTTGA72630.2142611236274588No Hit
GATACCACTGCTTATGTACTCTGCG70530.20806604776875495No Hit
GTACTGGTTCACTATCGGTCAGTCA68780.20290348455316837No Hit
GTTGATACCACTGCTTATGTACTCT68710.2026969820245449No Hit
ATACCACTGCTTATGTACTCTGCGT68150.20104496179555717No Hit
GAGTACATGATAAGCAGTGGTATCA59800.17641216016690123No Hit
GAGTACATGGGAAGCAGTGGTATCA59550.17567465113610317No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58420.17234111031689583No Hit
CAGTGGTATCAACGCAGAGTACATA54940.16207498460818653No Hit
GGATACCACGTGTCCCGCCCTACTC53360.15741392753354266No Hit
GCTTATCATGTACTCTGCGTTGATA50740.14968483289077875No Hit
TACCACTGCTTATGTACTCTGCGTT50520.14903582494367645No Hit
GGATAAGCAGTGGTATCAACGCAGA49430.1458202855693968No Hit
TGATACCACTGCTTATGTACTCTGC48840.14407976425671332No Hit
GTATCAACGCAGAGTACATGGGAAG48500.14307675197482791No Hit
ATACAGGGTGACAGCCCCGTACACA48130.14198523860924678No Hit
GCAGAGTACATAAGCAGTGGTATCA46090.13596716491793442No Hit
GCGTTGATACCACTGCTTCCCATGT45920.13546565877699174No Hit
GGTATCAACGCAGAGTACATGGGAA45460.13410864216032325No Hit
GATAAGCAGTGGTATCAACGCAGAG45250.13348913457445286No Hit
GCGTTGATACCACTGCTTATCATGT45080.13298762843351017No Hit
GGGATAAGCAGTGGTATCAACGCAG43380.1279725670240832No Hit
GAGTACATGGGATAAGCAGTGGTAT41540.12254450055740931No Hit
CCCCATGTACTCTGCGTTGATACCA40610.11980096696284047No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38560.1137533929102962No Hit
TATCAACGCAGAGTACATGGGAAGC38530.11366489182660042No Hit
CAGTGGTATCAACGCAGAGTACATG38510.11360589110413657No Hit
ACGCAGAGTACATGATAAGCAGTGG36570.10788282102514345No Hit
ATCATTAACTGAATCCATAGGTTAA36040.1063193018798515No Hit
GTACACAAGCAGTGGTATCAACGCA34860.10283825925448456No Hit
ACGCAGAGTACATGGGAAGCAGTGG34140.10071423324578609No Hit
ATCAACGCAGAGTACATAAGCAGTG34050.1004487299946988No Hit
ACTCTGCGTTGATACCACTGCTTCC33990.10027172782730726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA3700.014.88960214
TGTATCA5350.014.55850914
CTTCGTT4350.013.53814912
CAGTTGT5950.013.410295510
GTACAAA8350.013.3191591
GCAGTTG6200.012.8695589
GTTGTAT6400.012.61524712
TTGTATC6250.012.61405913
AGCAGTT6150.012.3567358
TTCGTTG4700.012.3278913
GTTATGG853.9214938E-612.3013031
GGTCGCT701.09244924E-412.2133087
GTAAGAC1251.4078978E-912.1626173
ACTCTTC5100.012.1065179
CCTACGA550.003066142612.0927982
GTCCTAG1501.4551915E-1112.0403671
AGTTGTA6650.011.99815511
TAGTACC1353.7107384E-1011.9657144
CCTATAA1209.942596E-911.8819411
GGTTATA1209.942596E-911.8819411
Produced by FastQC (version 0.11.5)